RESUMO
The somatotropic axis is a major regulatory pathway of energy metabolism during postnatal growth in mammals. Genes involved in this pathway influence many economically important traits. The association of selected SNPs in these genes with carcass traits was examined in grazing Brangus steers. These traits included final live weight, ultrasound backfat thickness (UBFT), rib-eye area, kidney fat weight, hot carcass weight, and intramuscular fat percentage (%IMF). Genomic DNA (N = 246) was genotyped for a panel of 15 tag SNPs located in the growth hormone receptor (GHR), insulin-like growth factor I, insulin-like growth factor-binding protein 6, pro-melanin-concentrating hormone, suppressor of cytokine signaling 2, and signal transducer and activator of transcription 6 (STAT6) genes. Allelic and haplotype frequencies were compared with those of a sample of European breeds (N = 177 steers). Two tag SNPs in the GHR affected %IMF; one of them (ss86273136) was also strongly associated with UBFT (P < 0.003). The frequency of the most favorable GHR haplotype for %IMF was lower in Brangus steers. Moreover, the haplotype carrying two unfavorable alleles was present at a frequency of 31% in this group. Four tag SNPs on STAT6 had a significant effect on UBFT. One of these, SNP ss115492467, was also associated with %IMF. The STAT6 haplotype, including all the alleles favoring UBFT, was the most abundant variant (34%) in the European cattle, while it had a frequency of 14% in the Brangus steers. The four less favorable variants (absent in the European cattle) were found at a frequency of 38% in the Brangus steers. These results support the association of GHR and STAT6 SNP with carcass traits in composite breeds, such as Brangus, under grazing conditions.
Assuntos
Composição Corporal/genética , Bovinos/anatomia & histologia , Estudos de Associação Genética , Marcadores Genéticos , Aumento de Peso/genética , Tecido Adiposo/química , Alelos , Animais , Argentina , Pesos e Medidas Corporais , Cruzamento , Bovinos/genética , Genótipo , Haplótipos , Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina/genética , Fator de Crescimento Insulin-Like I/genética , Carne , Músculo Esquelético/química , Fenótipo , Polimorfismo de Nucleotídeo Único , Receptores da Somatotropina/genética , Fator de Transcrição STAT6/genéticaRESUMO
The PPARGC1A gene (peroxysome proliferator-activated receptor-gamma coactivator 1alpha gene) controls muscle fiber type and brown adipocyte differentiation; therefore, it is a candidate gene for beef quality traits (tenderness and fat content). Two SNPs (Single Nucleotide Polymorphisms) were identified within exon 8 by multiple alignment of DNA sequences obtained from 24 bulls: a transition G/A (SNP 1181) and a transversion A/T (SNP 1299). The SNP 1181 is a novel SNP, corresponding to a non-conservative substitution (AGT/AAT) that could be the cause of amino acid substitution ((364)Serine/(364)Asparagine). A Mismatch PCR method was designed to determine genotypes of 73 bulls and 268 steers for SNP 1181. Growth, slaughter and meat quality information were available for the group of steers. Allele A of SNP 1181 was not found in Angus. In 243 steers, no significant differences (P > 0.05) were found for either final live body weight, gain in backfat thickness in Spring, kidney fat weight, kidney fat percentage, Warner-Bratzler shear force at 7 days postmortem, intramuscular fat percentage or meat colour between genotype GG and AG. This SNP could be included in breed composition and population admixture analyses because there are marked differences in allelic frequencies between Bos taurus and Bos indicus breeds.
Assuntos
Bovinos/genética , Polimorfismo de Nucleotídeo Único , Fatores de Transcrição/genética , Animais , Sequência de Bases , Peso Corporal/genética , Bovinos/classificação , Bovinos/crescimento & desenvolvimento , Feminino , Frequência do Gene , Variação Genética , Genótipo , Masculino , Carne/normas , Dados de Sequência Molecular , Fenótipo , Reação em Cadeia da Polimerase/métodos , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
Leptin is a hormone that affects the regulation of feed intake, energy balance and body composition in mammals. Several polymorphisms in the bovine leptin gene have been associated with phenotypic variance of these traits. We evaluated two known single nucleotide polymorphisms (SNPs) in the leptin gene of 253 grazing Brangus steers. Brangus is a 5/8 Angus-3/8 Brahman composite. Data were collected during two consecutive growth/fattening cycles from two farms in southeast Buenos Aires province, Argentina. One of the markers is in the promoter region of the gene (SNP1) and the other is a non-synonymous polymorphism in exon 2 (SNP2). The traits that we evaluated were live weight gain in the spring, gain in backfat thickness in the spring, final live weight, final ultrasound backfat thickness, final ultrasound rib eye area, carcass weight and length, carcass yield, kidney fat, kidney fat percentage, backfat thickness, rib eye area, and intramuscular fat percentage. Both markers affected some meat traits; though the only significant associations were of SNP1 with ultrasound rib eye area and of SNP2 with carcass yield and backfat thickness. Under the same conditions as in the present study, leptin markers could be of help only as part of a larger genotyping panel including other relevant genes.
Assuntos
Bovinos/crescimento & desenvolvimento , Leptina/genética , Polimorfismo Genético , Animais , Argentina , Composição Corporal , Bovinos/genética , Marcadores Genéticos , Genótipo , FenótipoRESUMO
To determine the production responses to rumen undegradable protein (RUP) feeding in grazing conditions, we fed 18 multiparous Holstein cows concentrates containing either soybean meal (SBM) or blood meal (BM) during the first 8 wk of lactation. One cow from the SBM treatment was removed because of mastitis. Six additional dairy cows in late lactation fitted with ruminal cannula were used to evaluate the rumen environment and the in situ crude protein (CP) degradability of concentrates. On a dry matter (DM) basis, concentrates contained SBM (33%) or BM (13%), corn grain (64 and 84% for SBM and BM, respectively) and a mineral-vitamin complex (3%). Concentrates were offered at a rate of 6.6 kg/d per cow and herbage allowance averaged 31 kg/d of DM per cow. The BM reduced ruminal ammonia-N levels and had no effect on ruminal pH and molar volatile fatty acid concentration. The degradable fraction (63.59 vs. 22.46%) and the rate of disappearance of the CP (9.68 vs. 1.69%/h) were greater for the SBM compared with the BM concentrate. Cows fed the BM concentrate produced more milk (29.3 vs. 24.9 kg/d) and more milk protein (0.85 vs. 0.74 kg/d) than did those fed the SBM concentrate. Milk fat yield and percentages of milk fat, lactose and protein were not affected. Forage DMI was increased by BM (17.19 vs. 13.17 kg/d per cow). The in vivo responsiveness to lipolytic stimuli were increased by BM but enhanced body weight loss or higher plasma nonesterified fatty acids concentration were not observed. Results indicated that a concentrate with a high RUP content increased milk and milk protein yields when spring pasture was the sole forage. The highest milk yield was more likely caused by increased DM than by enhanced body lipid mobilization.