RESUMO
Background: Hemoplasmas are eperythrocytic procaryotes, including Mycoplasma species which were recently transferred from the genera Eperythrozoon and Haemobartonella, based on their similarity of the 16S rRNA sequences, and newly identified hemotropic mycoplasmas. Eperythrozoonosis of pigs is caused by the haemotrophic bacterium M. suis, and the disease has a worldwide distribution. The disease manifests as a severe and often fatal acute febrile icteroanemia, mainly in piglets, pregnant sows before parturition and fattening pigs exposed to stress. The aim of this study was to determine the seroprevalence of IgG to M. suis in swine populations in Serbia, using a Western Blot test with the recombinant protein MSG1. Materials, Methods & Results: Four farms were chosen to represent the main swine-producing geographic regions of Serbia, including South Baka District (farms 1 and 2), North Baka District (farm 3) and Branievo District (farm 4). A total of forty-six clinically healthy pigs, age 8-20 weeks, were included in the study. Blood samples from pigs randomly selected from the four farms were collected by jugular vein puncture into serum vacutainer tubes with clot activator. After clotting at room temperature for 1 h, blood samples were centrifuged at 1500 g, for 15 min at room temperature. Sera were carefully harvested and stored at -20C until assayed. The sera samples
Background: Hemoplasmas are eperythrocytic procaryotes, including Mycoplasma species which were recently transferred from the genera Eperythrozoon and Haemobartonella, based on their similarity of the 16S rRNA sequences, and newly identified hemotropic mycoplasmas. Eperythrozoonosis of pigs is caused by the haemotrophic bacterium M. suis, and the disease has a worldwide distribution. The disease manifests as a severe and often fatal acute febrile icteroanemia, mainly in piglets, pregnant sows before parturition and fattening pigs exposed to stress. The aim of this study was to determine the seroprevalence of IgG to M. suis in swine populations in Serbia, using a Western Blot test with the recombinant protein MSG1. Materials, Methods & Results: Four farms were chosen to represent the main swine-producing geographic regions of Serbia, including South Baka District (farms 1 and 2), North Baka District (farm 3) and Branievo District (farm 4). A total of forty-six clinically healthy pigs, age 8-20 weeks, were included in the study. Blood samples from pigs randomly selected from the four farms were collected by jugular vein puncture into serum vacutainer tubes with clot activator. After clotting at room temperature for 1 h, blood samples were centrifuged at 1500 g, for 15 min at room temperature. Sera were carefully harvested and stored at -20C until assayed. The sera samples
RESUMO
Background: Hemoplasmas are eperythrocytic procaryotes, including Mycoplasma species which were recently transferred from the genera Eperythrozoon and Haemobartonella, based on their similarity of the 16S rRNA sequences, and newly identified hemotropic mycoplasmas. Eperythrozoonosis of pigs is caused by the haemotrophic bacterium M. suis, and the disease has a worldwide distribution. The disease manifests as a severe and often fatal acute febrile icteroanemia, mainly in piglets, pregnant sows before parturition and fattening pigs exposed to stress. The aim of this study was to determine the seroprevalence of IgG to M. suis in swine populations in Serbia, using a Western Blot test with the recombinant protein MSG1. Materials, Methods & Results: Four farms were chosen to represent the main swine-producing geographic regions of Serbia, including South Baka District (farms 1 and 2), North Baka District (farm 3) and Branievo District (farm 4). A total of forty-six clinically healthy pigs, age 8-20 weeks, were included in the study. Blood samples from pigs randomly selected from the four farms were collected by jugular vein puncture into serum vacutainer tubes with clot activator. After clotting at room temperature for 1 h, blood samples were centrifuged at 1500 g, for 15 min at room temperature. Sera were carefully harvested and stored at -20C until assayed. The sera samples
Background: Hemoplasmas are eperythrocytic procaryotes, including Mycoplasma species which were recently transferred from the genera Eperythrozoon and Haemobartonella, based on their similarity of the 16S rRNA sequences, and newly identified hemotropic mycoplasmas. Eperythrozoonosis of pigs is caused by the haemotrophic bacterium M. suis, and the disease has a worldwide distribution. The disease manifests as a severe and often fatal acute febrile icteroanemia, mainly in piglets, pregnant sows before parturition and fattening pigs exposed to stress. The aim of this study was to determine the seroprevalence of IgG to M. suis in swine populations in Serbia, using a Western Blot test with the recombinant protein MSG1. Materials, Methods & Results: Four farms were chosen to represent the main swine-producing geographic regions of Serbia, including South Baka District (farms 1 and 2), North Baka District (farm 3) and Branievo District (farm 4). A total of forty-six clinically healthy pigs, age 8-20 weeks, were included in the study. Blood samples from pigs randomly selected from the four farms were collected by jugular vein puncture into serum vacutainer tubes with clot activator. After clotting at room temperature for 1 h, blood samples were centrifuged at 1500 g, for 15 min at room temperature. Sera were carefully harvested and stored at -20C until assayed. The sera samples