RESUMO
OBJECTIVES: Few studies have been conducted in South America regarding the detection and genotyping of human papillomavirus (HPV) in viral warts of renal transplant recipients (RTRs). The characterization of the population most susceptible to the development of warts and the knowledge of the main HPV types in this environment prompted this study, which focuses on the detection and typing of HPV in RTRs in Brazil. METHODS: Fifty-eight patients with viral warts from the Hospital São Paulo/Federal University of São Paulo were included in this study. HPV was detected by polymerase chain reaction (PCR) using combinations of the following primers: PGMY 09/11, RK 91, CP 65/70, and CP 66/69. Restriction fragment length polymorphism and automated sequencing techniques were used for HPV typing. RESULTS: HPV was detected by PCR in 89.7% of viral wart samples. The most frequently detected HPV types included 57, 27, 1a, 2a, and 20. Other types of HPV-epidermodysplasia verruciformis were also detected, including 14, 15, 19, 20, 21, 23, 36, and 38. Rare HPV types were also detected in our environment, including RTR X1, RTR X7, and 100. The time after transplant was correlated with an increased number of lesions and beta papillomavirus genus infection. CONCLUSIONS: The HPV types detected in the RTR population were similar to those described in immunocompetent populations. However, the diversity of the HPV types identified and the number of lesions were increased in the RTR population.
Assuntos
Transplante de Rim/efeitos adversos , Papillomaviridae/classificação , Verrugas/virologia , Adolescente , Adulto , Idoso , Brasil , Estudos Transversais , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/genética , Papillomaviridae/isolamento & purificação , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Transplantados , Adulto JovemRESUMO
Studies were conducted to characterize the labor behavior in Curraleiro Pé-duro cows, a Locally adapted breed found in the semiarid region of Brazil, which is considered rustic and fertile. Eleven Curraleiro Pé-duro cows were kept in pasture and were Observed every 4 h to assess the beginning of birth, and subsequently were constantly monitored until the complete expulsion of the placenta, accordingly to the three different stages of labor. The cows were evaluated according to the following events: staring into the flank (SIF); licking vulva and lifting the tail (LVUT); head-Butts to the flank (HF); dripping colostrum (DC); lying down and standing (restlessness; LDS); duration of the first phase (Dur1P); amount of initial contractions (IC); time to onset of appearing of feet (TOAF); duration of calving (DC) and duration time from end of calving til l the expulsion of the placenta (DurExpP). The results are presented as mean ± standard deviation: SIF (4.00 ± 2.37); LVUT (2.38 ± 1.06); HF (1.80 ± 0.45); DC (1.20 ± 0.45); LDS (2.56 ± 1.33);IC (6.00 ± 2.9); Dur1F (71 ± 40 min); TOAF (7± 8 min); DC (25 ± 24 min); and DurExpP (228 ± 76 min). These results allow us to characterize the events that precede and accompany the moment of labor in Curraleiro Pé-duro cows.
Assuntos
Feminino , Animais , Bovinos , Bovinos/crescimento & desenvolvimento , Bovinos/embriologia , Bovinos/fisiologia , Comportamento Animal/classificação , Parto , Seleção GenéticaRESUMO
Studies were conducted to characterize the labor behavior in Curraleiro Pé-duro cows, a Locally adapted breed found in the semiarid region of Brazil, which is considered rustic and fertile. Eleven Curraleiro Pé-duro cows were kept in pasture and were Observed every 4 h to assess the beginning of birth, and subsequently were constantly monitored until the complete expulsion of the placenta, accordingly to the three different stages of labor. The cows were evaluated according to the following events: staring into the flank (SIF); licking vulva and lifting the tail (LVUT); head-Butts to the flank (HF); dripping colostrum (DC); lying down and standing (restlessness; LDS); duration of the first phase (Dur1P); amount of initial contractions (IC); time to onset of appearing of feet (TOAF); duration of calving (DC) and duration time from end of calving til l the expulsion of the placenta (DurExpP). The results are presented as mean ± standard deviation: SIF (4.00 ± 2.37); LVUT (2.38 ± 1.06); HF (1.80 ± 0.45); DC (1.20 ± 0.45); LDS (2.56 ± 1.33);IC (6.00 ± 2.9); Dur1F (71 ± 40 min); TOAF (7± 8 min); DC (25 ± 24 min); and DurExpP (228 ± 76 min). These results allow us to characterize the events that precede and accompany the moment of labor in Curraleiro Pé-duro cows.(AU)