RESUMO
Pacas (Cuniculus paca) are highly hunted animals because of the flavor of their meat, and commercial breeding is recommended. However, this species has a relatively low reproductive rate. This study aimed to collect semen from pacas through electroejaculation and obtain the sperm parameters of this species for the first time. Seven male pacas were used, submitted to an anesthetic protocol before stimulation by an electroejaculator appropriate for the species. The stimulus protocol was performed in three series: series I, 10 stimuli with 1 and 2 V; series II, 10 stimuli with 3 and 4 V; and series III, 10 stimuli with 5 V and interval between series of 2 s. The collected material was evaluated for color, volume, motility, vigor, and concentration. The sperm parameters collected showed a mean volume of 0.43±0.33 mL, concentration of 45.5±42.44×106 sperm/mL, motility of 33.33±32.14%, and mean vigor of 2.6±1.15. In this study, the anesthetic protocol did not seem to favor semen collection by electroejaculation in the pacas. The electrical stimulation protocol was able to stimulate all animals in the study; however, there were few samples with sperm cells and a low rate of motility and vigor in most ejaculates.
Pacas (Cuniculus paca) são animais altamente caçados devido ao sabor de sua carne, sendo a criação comercial delas, recomendada. Entretanto, é uma espécie com baixa taxa reprodutiva. O objetivo deste estudo foi efetuar coleta de sêmen em pacas por meio da eletroejaculação e da obtenção, pela primeira vez, dos parâmetros espermáticos dessa espécie. Foram utilizadas sete pacas machos, as quais, antes dos estímulos por eletroejaculador apropriado para a espécie, foram submetidas a protocolo anestésico. O protocolo de estímulo foi realizado em três séries: série I, 10 estímulos com 1 e 2V; série II, 10 estímulos com 3 e 4V; série III, 10 estímulos com 5V, e intervalo entre as séries de dois segundos. O material coletado foi avaliado quanto à cor, ao volume, à motilidade, ao vigor e à concentração. Os parâmetros espermáticos coletados demonstraram volume médio de 0,43±0,33mL, concentração de 45,5±42,44x106 espermatozoides/mL, motilidade de 33,33±32,14% e vigor médio de 2,6±1,15. Neste estudo, o protocolo anestésico parece não ter favorecido a coleta de sêmen por eletroejaculação em pacas. Entretanto, o protocolo de estímulos elétricos foi capaz de estimular todos os animais do estudo. Assim, chegou-se ao resultado de poucas amostras com a presença de células espermáticas, bem como baixo índice de motilidade e vigor na maioria dos ejaculados.
Assuntos
Animais , Reprodução , Sêmen , Biotecnologia , CuniculidaeRESUMO
Dentinogenesis imperfecta (DI) is the main orodental manifestation of osteogenesis imperfecta (OI) caused by COL1A1 or COL1A2 heterozygous pathogenic variants. Its prevalence varies according to the studied population. Here, we report the molecular analysis of 81 patients with OI followed at reference centers in Brazil and France presenting COL1A1 or COL1A2 variants. Patients were submitted to clinical and radiographic dental examinations to diagnose the presence of DI. In addition, a systematic literature search and a descriptive statistical analysis were performed to investigate OI/DI phenotype-genotype correlation in a worldwide sample. In our cohort, 50 patients had COL1A1 pathogenic variants, and 31 patients had COL1A2 variants. A total of 25 novel variants were identified. Overall, data from a total of 906 individuals with OI were assessed. Results show that DI was more frequent in severe and moderate OI cases. DI prevalence was also more often associated with COL1A2 (67.6%) than with COL1A1 variants (45.4%) because COL1A2 variants mainly lead to qualitative defects that predispose to DI more than quantitative defects. For the first time, 4 DI hotspots were identified. In addition, we showed that 1) glycine substitution by branched and charged amino acids in the α2(I) chain and 2) substitutions occurring in major ligand binding regions-MLRB2 in α1(I) and MLBR 3 in α2(I)-could significantly predict DI (P < 0.05). The accumulated variant data analysis in this study provides a further basis for increasing our comprehension to better predict the occurrence and severity of DI and appropriate OI patient management.
Assuntos
Cadeia alfa 1 do Colágeno Tipo I , Colágeno Tipo I , Dentinogênese Imperfeita , Osteogênese Imperfeita , Humanos , Colágeno Tipo I/genética , Dentinogênese Imperfeita/genética , Estudos de Associação Genética , Mutação , Osteogênese Imperfeita/diagnóstico por imagem , Osteogênese Imperfeita/genéticaRESUMO
Studies aiming at the development and evaluation of alternative methods to minimise losses caused by the gastrointestinal nematode Haemonchus contortus are extremely important. Such research is essential, given the high morbidity rates among sheep and the significant mortality rates of lambs, allied to the low efficacy of commercial products for the control of this parasite. The purpose of this study was to evaluate the effect of the Saccharomyces cerevisiae (YT001 - YEASTECH) on the control of H. contortus and its modulation of the immune response in experimentally infected sheep. Eighteen sheep were divided into two groups. Group 1, the control group, comprised animals infected with H. contortus and supplemented with distilled water, while Group 2, the treated group, consisted of animals infected and supplemented with S. cerevisiae (400 million cfu/day of suspension for 49 days). The following parasitological parameters were evaluated: number of eggs per gram of faeces, number of infective larvae (L3) recovered per faecal culture, and parasitic load of the abomasum. The following immunological parameters were quantified: immunoglobulin (Ig)A in the mucous secretions and serum IgG; cytokines interleukin (IL)-4, IL-5 and IL-10; number of eosinophils in the abomasal mucosa and groups of cells positive for the markers: MHCII, CD4+CD25+, CD5+CD8+, WC4, CD5+CD4+, CD8+CD11b+ and CD5+WC1 by whole blood flow cytometry. The results revealed a significant decrease (P<0.05) in the number of larvae and significantly higher serum IgG levels (P<0.05) in the group supplemented with S. cerevisiae. The supplemented animals showed significantly larger numbers of eosinophils (P<0.05), as well as more cells positive for MHCII, CD4+CD25+, CD5+CD8+ than the control animals. This study confirmed the beneficial action of S. cerevisiae on the host immune response to H. contortus, as evidenced mainly by the smaller number of L3 recovered from the faeces of sheep supplemented with S. cerevisiae.
Assuntos
Suplementos Nutricionais/microbiologia , Hemoncose/veterinária , Probióticos/administração & dosagem , Saccharomyces cerevisiae/imunologia , Doenças dos Ovinos/terapia , Ovinos/imunologia , Administração Oral , Animais , Anticorpos Anti-Helmínticos/sangue , Citocinas/imunologia , Eosinófilos/imunologia , Fezes/parasitologia , Hemoncose/imunologia , Hemoncose/terapia , Haemonchus , Interações entre Hospedeiro e Microrganismos/imunologia , Imunoglobulina A/análise , Imunoglobulina G/sangue , Contagem de Leucócitos , Masculino , Contagem de Ovos de Parasitas , Ovinos/parasitologia , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/parasitologiaRESUMO
AIM: To screen and identify a potential biosurfactant-producing yeast strain isolated from Antarctic soil and to evaluate the fermentation process kinetics of the most promising strain on biosurfactant production using glycerol as carbon source. METHODS AND RESULTS: From the 68 isolated yeast strains, 11 strains were able to produce biosurfactants after Emulsification Index (E.I.) and Drop Collapse tests, reaching an E.I. higher than 10%. Strain 1_4.0 was the best producer, identified as Candida glaebosa based on molecular analysis. Yeast was cultivated in a medium composed of glycerol supplemented with yeast extract for 120 h to determine the process kinetics. The increased C/N ratio affected yeast growth and biosurfactant production. Biosurfactant release was associated with the end of exponential and beginning of the stationary growth phases. Results indicated an E.I. of 30% at the end of the fermentation. CONCLUSIONS: The feasiability of C. glaebosa to produce biosurfactant from a low-cost medium cultivation shows a great impact on the development of bioresource in the Antarctica terrestrial environment. SIGNIFICANCE AND IMPACT OF THE STUDY: Although the diversity of psychrophilic/psychrotolerant micro-organisms from Antarctica has been the preferred subject of study by microbiologists, terrestrial microfungal communities are scarcely investigated and literature about the biotechnological potential of such micro-organisms should cover important biomolecules in addition to cold-adapted enzymes. In the present study, for the first time, the Maritime Antarctica environment was screened as a novel source of biosurfactants produced by micro-organisms.
Assuntos
Candida/metabolismo , Microbiologia do Solo , Tensoativos/metabolismo , Regiões Antárticas , Candida/isolamento & purificação , Glicerol/metabolismo , Ilhas , Cinética , Tensoativos/isolamento & purificação , Leveduras/crescimento & desenvolvimento , Leveduras/isolamento & purificação , Leveduras/metabolismoRESUMO
The objective of this study was to evaluate the cytotoxic activity of rosemary (REO, Rosmarinus officinalis L.), turmeric (CEO, Curcuma longa L.), and ginger (GEO, Zingiber officinale R.) essential oils in HeLa cells. Cytotoxicity tests were performed in vitro, using tetrazolium (MTT) and neutral red assays for evaluation of antiproliferative activity by different mechanisms, trypan blue assay to assess cell viability and evaluation of cell morphology for Giemsa to observe the cell damage, and Annexin V to evaluate cell death by apoptosis. CEO and GEO exhibited potent cytotoxic activity against HeLa cells. IC50 obtained was 36.6 µg/mL for CEO and 129.9 µg/mL for GEO. The morphology of HeLa cells showed condensation of chromatin, loss of cell membrane integrity with protrusions (blebs), and cell content leakage for cells treated with CEO and GEO, from the lowest concentrations studied, 32.81 µg/mL of CEO and 32.12 µg/mL of GEO. The Annexin V assay revealed a profile of cell death by apoptosis for both CEO and GEO. The results indicate cytotoxic activity in vitro for CEO and GEO, suggesting potential use as anticancer agents for cervical cancer cells.
Assuntos
Curcuma/química , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Rosmarinus/química , Zingiber officinale/química , Antineoplásicos Fitogênicos/farmacologia , Células HeLa , HumanosRESUMO
These experiments were performed to determine the chemical composition, coefficients of nutrient and energy metabolizability, amino acid composition, and cytotoxicity of different castor oil meals subjected to different detoxification processes and added to the diet of Japanese quails. In the trial, 180 46-d-old female Japanese quails were distributed according to a completely randomized design into five treatments and with replicates of six bird each. The treatments consisted of following detoxification methods of castor oil meal: Castor oil meal A (CMA) - recovery in alcohol at 80 °C for 20 minutes and drying at 80 °C; castor oil meal B (CMB) and C (CMC) - recovery in alcohol at 80 °C for 6 minutes, neutralization with 5% NaOH, and drying under direct sunlight sun for two days (CMB) or pelleted (CMC); castor oil meal D (CMD) - recovery in alcohol at 110 °C for 15 minutes and drying at 110 °C. Castor oil meal was added replacing 20% of the reference diet. There was slight chemical composition variation (1.21% in crude protein, 6% in dry matter, 2.2% in ether extract and 64 kcal/kg in gross energy) among the castor oil meals submitted to the different treatments. The castor oil meal submitted to treatment C showed the highest amino acid values. In the cytotoxicity test, treatment D presented lower ricin activity. Castor oil meals A, C, and D may be included in Japanese quail diets; however, castor oil meal D is recommended due to the simplicity its industrial process, its low toxicity, and metabolizability coefficients obtained.
Assuntos
Animais , Citotoxicidade Imunológica , Coturnix/classificação , Coturnix/metabolismo , Ração Animal/análise , Ração Animal/classificaçãoRESUMO
These experiments were performed to determine the chemical composition, coefficients of nutrient and energy metabolizability, amino acid composition, and cytotoxicity of different castor oil meals subjected to different detoxification processes and added to the diet of Japanese quails. In the trial, 180 46-d-old female Japanese quails were distributed according to a completely randomized design into five treatments and with replicates of six bird each. The treatments consisted of following detoxification methods of castor oil meal: Castor oil meal A (CMA) - recovery in alcohol at 80 °C for 20 minutes and drying at 80 °C; castor oil meal B (CMB) and C (CMC) - recovery in alcohol at 80 °C for 6 minutes, neutralization with 5% NaOH, and drying under direct sunlight sun for two days (CMB) or pelleted (CMC); castor oil meal D (CMD) - recovery in alcohol at 110 °C for 15 minutes and drying at 110 °C. Castor oil meal was added replacing 20% of the reference diet. There was slight chemical composition variation (1.21% in crude protein, 6% in dry matter, 2.2% in ether extract and 64 kcal/kg in gross energy) among the castor oil meals submitted to the different treatments. The castor oil meal submitted to treatment C showed the highest amino acid values. In the cytotoxicity test, treatment D presented lower ricin activity. Castor oil meals A, C, and D may be included in Japanese quail diets; however, castor oil meal D is recommended due to the simplicity its industrial process, its low toxicity, and metabolizability coefficients obtained.(AU)
Assuntos
Animais , Coturnix/classificação , Coturnix/metabolismo , Citotoxicidade Imunológica , Ração Animal/análise , Ração Animal/classificaçãoRESUMO
An experiment was carried out to evaluate the effect of environmental enrichment in a free-range chicken production system on live performance as a function of microclimate, physiological parameters, and performance parameters. Four production modules were divided into four pens with 10 birds each, totaling 60 birds. The following treatments were applied: access to a paddock (TEST), access to a paddock with perches (PER), access to a paddock with artificial shade (SHA), and access to the paddock with perches and artificial shade (PESH). The PESH production module presented the best globe temperature (Tbg,ºC) and enthalpy (h, kJ/kg), and thereby, the best thermal environmental conditions, which ensured the longest permanence time of the birds in the paddock. The SHA and PESH modules promoted the lowest respiratory rate and shank and comb temperatures. Live performance was influenced by the presence of environmental enrichment (modules SHA and PESH), with the highest live weight (LW) and weight gain (WG) and the lowest feed conversion ratio (FCR) and metabolizable energy intake (MEI). Parts yield, such as giblets, were not influenced by production modules, except for PESH, which promoted higher offal weight. In general, chickens reared in enriched production modules presented greatest performance and comfort results and were considered close to optimal rearing conditions.(AU)
Assuntos
Animais , Galinhas/crescimento & desenvolvimento , Comportamento Animal , Produtos Avícolas/análise , Galinhas/fisiologiaRESUMO
An experiment was carried out to evaluate the effect of the inclusion of 20% whole-grain or ground pearl millet (PM) in mash and pelleted diets on the performance, carcass traits, and organ weights of broilers reared until 21 days of age. A randomized block experimental design in a 3 x 2 factorial arrangement (diets containing corn and soybean meal, whole-grain PM, or ground PM x mash or pelleted diets), with five replicates per treatment and 10 birds per experimental unit, was applied. Diets were analyzed for mean geometric diameter, geometric standard deviation, pellet hardness, and density. Broiler performance, carcass yield, and organ weights were evaluated. On day 21, one bird with the average weight of each experimental unit was sacrificed for carcass evaluation. It was concluded that both as whole-grain and ground PM can be added to the diet of broilers up to 21 days of age. The dietary inclusion of PM results in higher abdominal fat deposition. Broilers fed the pelleted diets presented lower feed intake, better feed conversion ratio, lower gizzard and heart percentages, and higher carcass weight.
Assuntos
Animais , Aumento de Peso , Composição Corporal , Galinhas/fisiologia , Galinhas/metabolismo , Milhetes , Ração AnimalRESUMO
An experiment was carried out to evaluate the effect of the inclusion of 20% whole-grain or ground pearl millet (PM) in mash and pelleted diets on the performance, carcass traits, and organ weights of broilers reared until 21 days of age. A randomized block experimental design in a 3 x 2 factorial arrangement (diets containing corn and soybean meal, whole-grain PM, or ground PM x mash or pelleted diets), with five replicates per treatment and 10 birds per experimental unit, was applied. Diets were analyzed for mean geometric diameter, geometric standard deviation, pellet hardness, and density. Broiler performance, carcass yield, and organ weights were evaluated. On day 21, one bird with the average weight of each experimental unit was sacrificed for carcass evaluation. It was concluded that both as whole-grain and ground PM can be added to the diet of broilers up to 21 days of age. The dietary inclusion of PM results in higher abdominal fat deposition. Broilers fed the pelleted diets presented lower feed intake, better feed conversion ratio, lower gizzard and heart percentages, and higher carcass weight.(AU)
Assuntos
Animais , Galinhas/metabolismo , Galinhas/fisiologia , Ração Animal , Milhetes , Composição Corporal , Aumento de PesoRESUMO
Aspergillus flavus, a haploid organism found worldwide in a variety of crops, including maize, cottonseed, almond, pistachio, and peanut, causes substantial and recurrent worldwide economic liabilities. This filamentous fungus produces aflatoxins (AFLs) B1 and B2, which are among the most carcinogenic compounds from nature, acutely hepatotoxic and immunosuppressive. Recent efforts to reduce AFL contamination in crops have focused on the use of nonaflatoxigenic A. flavus strains as biological control agents. Such agents are applied to soil to competitively exclude native AFL strains from crops and thereby reduce AFL contamination. Because the possibility of genetic recombination in A. flavus could influence the stability of biocontrol strains with the production of novel AFL phenotypes, this article assesses the diversity of vegetative compatibility reactions in isolates of A. flavus to identify heterokaryon self-incompatible (HSI) strains among nonaflatoxigenic isolates, which would be used as biological controls of AFL contamination in crops. Nitrate nonutilizing (nit) mutants were recovered from 25 A. flavus isolates, and based on vegetative complementation between nit mutants and on the microscopic examination of the number of hyphal fusions, five nonaflatoxigenic (6, 7, 9 to 11) and two nontoxigenic (8 and 12) isolates of A. flavus were phenotypically characterized as HSI. Because the number of hyphal fusions is reduced in HSI strains, impairing both heterokaryon formation and the genetic exchanges with aflatoxigenic strains, the HSI isolates characterized here, especially isolates 8 and 12, are potential agents for reducing AFL contamination in crops.
Assuntos
Aflatoxinas/análise , Aspergillus flavus/fisiologia , Contaminação de Alimentos/prevenção & controle , Microbiologia de Alimentos , Aflatoxinas/biossíntese , Aflatoxinas/genética , Arachis/microbiologia , Aspergillus flavus/genética , Aspergillus flavus/metabolismo , Variação Genética , Recombinação Genética , Zea mays/microbiologiaRESUMO
In this Letter, low-frequency photorefractive holographic moiré fringe patterns are proposed as secure numerical code generators that could be useful for storage or data transmission. These dynamic moiré patterns are holographically obtained by the superposition of two or more sinusoidal gratings with slightly different pitches. The Bi(12)TiO(20) photorefractive crystal sample is used as holographic medium. An optical numerical base was defined with patterns representing the 0, 1 and -1 digits as bits. Then, the complete set of these optical bits is combined to form bytes, where a numerical sequence is represented. The results show that the proposed numerical code is simple, robust and extremely secure, then could be used efficiently as standard numerical identification in robotic vision or eventually in storage or transmission of secure numerical data.
RESUMO
The use of efficient breeding methods depends on knowledge of genetic control of traits to be improved. We estimated genetic parameters, selection gain, and genetic diversity in physic nut half-sib families, in order to provide information for breeding programs of this important biofuel species. The progeny test included 20 half-sib families in 4 blocks and 10 plants per plot. The mean progeny heritability values were: 50% for number of bunches, 47% for number of fruits, 35% for number of seeds, 6% for stem diameter, 26% for number of primary branches, 14% for number of secondary branches, 66% for plant height, and 25% for survival of the plants, demonstrating good potential for early selection in plant height, number of branches, and number of fruits per plant. In the analysis of genetic diversity, genotypes were divided into 4 groups. Genotypes 18, 19, 20, and 8 clustered together and presented the highest means for the vegetative and production. Lower means were observed in the 17, 12, 13, and 9 genotypes from the same group. We detected genetic variability in this population, with high heritability estimates and accuracy, demonstrating the possibility of obtaining genetic gains for vegetative characters and production at 24 months after planting.
Assuntos
Variação Genética , Jatropha/genética , Seleção Genética , Cruzamento , Jatropha/anatomia & histologia , Componentes Aéreos da Planta/anatomia & histologia , Característica Quantitativa HerdávelRESUMO
Imidocarb dipropionate (IMD) is a chemotherapeutic agent prescribed for the treatment and control of babesiosis; it is known to be a nucleic acid synthesis inhibitor. Although it is an effective babesicide, there are reports of persistent IMD residues retained at high levels in edible tissues of cattle, swine and sheep, raising concerns about potential effects on humans. Since the carcinogenic potential of a chemical compound can be assessed through its effect on the homologous recombination, we investigated whether IMD is recombinogenic in Aspergillus nidulans diploid cells and whether it is capable of inducing homozygosis in genes that were previously heterozygous. This analysis was done with a homozygotization assay applied to a heterozygous diploid strain of A. nidulans. IMD used at non-toxic concentrations (2.5 to 10.0 µM) was recombinogenic, demonstrated by homozygotization indices higher than 2.0 for diploid markers. A diploid homozygous for genetic markers from chromosomes I and II was also produced. Since DNA replication blockers that induce DNA strand breaks have been classified as potent inducers of homologous recombination, the recombinogenic potential of IMD may be due to induction of recombinational repair.
Assuntos
Antiprotozoários/farmacologia , Aspergillus nidulans/citologia , Aspergillus nidulans/genética , Diploide , Imidocarbo/análogos & derivados , Mitose/efeitos dos fármacos , Recombinação Genética/efeitos dos fármacos , Animais , Aspergillus nidulans/efeitos dos fármacos , Babesia/efeitos dos fármacos , Bovinos , Cromossomos Fúngicos/genética , Troca Genética/efeitos dos fármacos , Genótipo , Imidocarbo/farmacologiaRESUMO
AIM: To evaluate the association of the polar and lateral flagella with biofilm formation on plastic surfaces in 76 Aeromonas caviae strains isolated from environment (lagoon water), food (vegetables, fish and cheese) and human source (faeces). METHODS AND RESULTS: Both polar (flaA) and lateral (lafA) flagellin genes have been investigated by means of PCR and colony blot hybridization assays. The ability to form biofilm in polystyrene microtitre plates was evaluated and correlated with the presence and absence from these genes. The flaA and lafA genes had a frequency of 94% and 71%, respectively. All lafA(+) strains were also flaA(+) . Biofilm formation was observed in 72% of strains. Ninety-four per cent of flaA(+) lafA(+) strains could form biofilm and those that presented an intense biofilm production harboured both genes. All flaA(-) lafA(-) isolates, as well as 76% of flaA(+) lafA(-) strains, were incapable of forming biofilm. All the fish strains were flaA(+) lafA(+) and displayed higher biofilm formation (88%). Lagoon water samples exhibited lower positivity rate for the lafA gene (57%) and decreased ability to produce biofilm (39%). CONCLUSIONS: Both polar and lateral flagellar function contribute to biofilm formation in Aer. caviae strains. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides evidence for the association of both flagella with biofilm formation, a factor required for pathogenicity of Aer. caviae strains of varied sources, especially food and human.
Assuntos
Aeromonas caviae/crescimento & desenvolvimento , Aeromonas caviae/genética , Biofilmes/crescimento & desenvolvimento , Flagelina/genética , Flagelina/metabolismo , Aeromonas caviae/patogenicidade , Aderência Bacteriana/genética , Flagelos/genética , Flagelos/metabolismo , Genótipo , Humanos , PlásticosRESUMO
Foram avaliadas possíveis alterações cardiorrespiratórias e no índice biespectral em cães anestesiados pelo isofluorano, associado ou não ao tramadol. Utilizaram-se 16 animais, distribuídos em dois grupos denominados GC (grupo-controle) e GT (grupo tramadol). Todos os cães foram induzidos e mantidos sob anestesia com isofluorano. Os animais do GC receberam 0,05ml/kg de solução salina a 0,9 por cento e os do GT 2mg/kg de tramadol, ambos por via intramuscular. Foram avaliados: freqüência cardíaca, pressão arterial sistólica, diastólica e média, eletrocardiografia, freqüência respiratória, saturação de oxiemoglobina, concentração de dióxido de carbono ao final da expiração, índice biespectral e recuperação da anestesia. Concluiu-se que a administração de tramadol em cães anestesiados pelo isofluorano não produz alterações nas variáveis cardiorrespiratórias, no índice biespectral e no tempo de recuperação da anestesia, porém proporciona boa qualidade de recuperação anestésica.
It was studied fortuitous cardiorespiratory and bispectral index changes in dogs anesthetized with isoflurane associated or not to tramadol. Sixteen dogswere distributed in two groups named CG (control group) and TG (tramadol group). General anesthesia was induced in all animals with isoflurane via mask. After 10 minutes, the animals of CG received 0.05ml/kg of saline solution at 0.9 percent, and TG received 2mg/kg of tramadol, both via intramuscular. It was evaluated heart rate, systolic, diastolic and mean arterial pressures; electrocardiography; respiratory rate; oxihemoglobin saturation; end tidal carbon dioxide; bispectral index and recovery of anesthesia. The administration of tramadol in dogs anesthetized with isoflurane did not produce changes in cardiorespiratory variables, bispectral index and anesthetic recovery time. In addition, this association promoted good quality of anesthetic recovery.
Assuntos
Animais , Análise Espectral/métodos , Anestésicos/administração & dosagem , Cães , Eletrocardiografia/métodos , Sistema Cardiovascular/metabolismo , Tramadol/administração & dosagem , Tramadol/análise , Tramadol/efeitos adversosRESUMO
Foram avaliadas possíveis alterações cardiorrespiratórias e no índice biespectral em cães anestesiados pelo isofluorano, associado ou não ao tramadol. Utilizaram-se 16 animais, distribuídos em dois grupos denominados GC (grupo-controle) e GT (grupo tramadol). Todos os cães foram induzidos e mantidos sob anestesia com isofluorano. Os animais do GC receberam 0,05ml/kg de solução salina a 0,9 por cento e os do GT 2mg/kg de tramadol, ambos por via intramuscular. Foram avaliados: freqüência cardíaca, pressão arterial sistólica, diastólica e média, eletrocardiografia, freqüência respiratória, saturação de oxiemoglobina, concentração de dióxido de carbono ao final da expiração, índice biespectral e recuperação da anestesia. Concluiu-se que a administração de tramadol em cães anestesiados pelo isofluorano não produz alterações nas variáveis cardiorrespiratórias, no índice biespectral e no tempo de recuperação da anestesia, porém proporciona boa qualidade de recuperação anestésica.(AU)
It was studied fortuitous cardiorespiratory and bispectral index changes in dogs anesthetized with isoflurane associated or not to tramadol. Sixteen dogswere distributed in two groups named CG (control group) and TG (tramadol group). General anesthesia was induced in all animals with isoflurane via mask. After 10 minutes, the animals of CG received 0.05ml/kg of saline solution at 0.9 percent, and TG received 2mg/kg of tramadol, both via intramuscular. It was evaluated heart rate, systolic, diastolic and mean arterial pressures; electrocardiography; respiratory rate; oxihemoglobin saturation; end tidal carbon dioxide; bispectral index and recovery of anesthesia. The administration of tramadol in dogs anesthetized with isoflurane did not produce changes in cardiorespiratory variables, bispectral index and anesthetic recovery time. In addition, this association promoted good quality of anesthetic recovery.(AU)
Assuntos
Animais , Sistema Cardiovascular/metabolismo , Tramadol/efeitos adversos , Tramadol/análise , Tramadol/administração & dosagem , Anestésicos/administração & dosagem , Análise Espectral/métodos , Eletrocardiografia/métodos , CãesRESUMO
We present a study of spatial structures created by superposition of spiral zone plates used for generating optical beams with phase singularities. Moiré fringes are observed that show topological defects similar to those appearing in interference patterns of optical vortices. A brief theoretical discussion is included that supports the similarities between the two phenomena. Our results may lead to interesting applications to digital information processing by optical means.
RESUMO
The aim of the present study is to assess the behaviour of different motivation methods on levels of oral hygiene among schoolchildren aged from 7 to 9 years in Araraquara, SP, Brazil. The methods tested were: indirect instruction using 'The Smiling Robot' (group I), indirect instruction through class presentation (group II) and direct instruction with macromodels (group III). A control group was also constituted, which received no kind of motivation (group IV). The O'Leary Plaque Index was used as the evaluation method, applied before the instruction and 30 days after application of the different methods. It was noted that the plaque index had not decreased in group IV only. In conclusion, all the motivation methods promoted significant decrease of plaque index and among these methods, the 'The Smiling Robot' was the one that provided the best results.