RESUMO

PURPOSE: To determine levels of Peptidyl arginine deiminase 2 (PAD2) and its product protein-bound citrulline in cadaver eyes that suffered from normal tension glaucoma (NTG) compared to primary open angle glaucoma (POAG), and controls. METHODS: Western analysis, ELISA, and immunohistochemical analysis were performed with human tissues. RESULTS: We report over expression of PAD2 and higher levels of its product protein-bound citrulline in the optic nerve of normal tension glaucoma patients (NTG). CONCLUSIONS: This is the first report demonstrating that like in POAG, NTG also possesses elevated levels of both PAD2 and protein-bound citrulline.

Assuntos

Glaucoma/enzimologia , Glaucoma/patologia , Hidrolases/metabolismo , Nervo Óptico/enzimologia , Nervo Óptico/patologia , Idoso , Idoso de 80 Anos ou mais , Citrulina/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Proteína-Arginina Desiminase do Tipo 2 , Desiminases de Arginina em Proteínas , Doadores de Tecidos