RESUMO
Ocean contamination mostly comes from anthropogenic contamination in watercourses. However, what happens in desert areas where watercourses are few or nonexistent? Are these coastal areas exempt from contamination? Do the remote locations of desert areas enable pristine coastal areas? Atacama is widely known for its desert aridity and mining resources; however, human impacts in its coastal areas have not been widely studied. Coastal zone uses of this region of Chile were analyzed per province in relation to the population settlements and economic activities on the coastal edge. This study includes a review of the contamination in this desert coastal area in relation to the territorial organization, activities, and land uses. The results determined that most of the coastal edge was used for fishing and aquaculture (52%) and for conservation and protected areas (39%). However, 2% of the coastal edge was susceptible to conflicts due to shared uses. A strong lack of scientific research was detected despite environmental interest (Humboldt Current, diversity hot spots, desert blooming, algae kelps, protected areas, etc.) and economic development (impact of mining, agriculture and tourism) in the area. Most studies focused on metal concentrations in aquatic environments in the north part of the region. Studies on emerging contaminants have not been carried out in the area despite intense human settlement.
Assuntos
Desenvolvimento Econômico , Efeitos Antropogênicos , Aquicultura , Chile , Conservação dos Recursos Naturais , Clima Desértico , Humanos , CaçaRESUMO
Two marine bacterial populations (Roseobacter sp. and Pseudomonas litoralis) were exposed to different concentrations of zinc (300, 625, 1250, 2000, 2500 and 5000â¯mgâ¯L-1) and cadmium (75, 250, 340, 500 and 1000â¯mgâ¯L-1) using two culture media (full nutrient Marine Broth 2216 "MB" and 1:10 (vol/vol) dilution with seawater of Marine Broth 2216 "MBSW"), in order to assess population responses depending on the culture medium and also potential adverse effects associated with these two metals. Different responses were found depending on the culture medium (Bacterial abundance (cells·mL-1), growth rates (µ, hours-1), and production of Extracellular Polysaccharides Substances (EPS) (µg glucose·cells-1). Results showed negative effects in both strains after the exposure to Zn treatments. Both strains showed highest metal sensitivity at low concentrations using both culture media. However, different results were found when exposing the bacterial populations to Cd treatments depending on the culture medium. Highest toxicity was observed using MB at low levels of Cd concentrations, whereas MBSW showed toxicity to bacteria at higher concentrations of Cd. Results not only showed adverse effects on Roseobacter sp. and Pseudomonas litoralis associated with the concentration of Zn and Cd, but also confirm that depending on the culture medium results can differ. This work suggests MBSW as an adequate culture medium to study metal toxicity bioassays in order to predict realistic effects on marine bacterial populations.
Assuntos
Bactérias/efeitos dos fármacos , Cádmio/toxicidade , Meios de Cultura/farmacologia , Água do Mar/química , Testes de Toxicidade/métodos , Poluentes Químicos da Água/análise , Zinco/toxicidade , Bactérias/crescimento & desenvolvimento , Exposição Ambiental/análise , Medição de RiscoRESUMO
In the retina information decoding is dependent on excitatory neurotransmission and is critically modulated by AMPA glutamate receptors. The Src-tyrosine kinase has been implicated in modulating neurotransmission in CNS. Thus, our main goal was to correlate AMPA-mediated excitatory neurotransmission with the modulation of Src activity in retinal neurons. Cultured retinal cells were used to access the effects of AMPA stimulation on nitric oxide (NO) production and Src phosphorylation. 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate fluorescence mainly determined NO production, and immunocytochemistry and Western blotting evaluated Src activation. AMPA receptors activation rapidly up-regulated Src phosphorylation at tyrosine 416 (stimulatory site) and down-regulated phosphotyrosine 527 (inhibitory site) in retinal cells, an effect mainly mediated by calcium-permeable AMPA receptors. Interestingly, experiments confirmed that neuronal NOS was activated in response to calcium-permeable AMPA receptor stimulation. Moreover, data suggest NO pathway as a key regulatory signaling in AMPA-induced Src activation in neurons but not in glial cells. The NO donor SNAP (S-nitroso-N-acetyl-DL-penicillamine) and a soluble guanylyl cyclase agonist (YC-1) mimicked AMPA effect in Src Tyr-416 phosphorylation, reinforcing that Src activation is indeed modulated by the NO pathway. Gain and loss-of-function data demonstrated that ERK is a downstream target of AMPA-induced Src activation and NO signaling. Furthermore, AMPA stimulated NO production in organotypic retinal cultures and increased Src activity in the in vivo retina. Additionally, AMPA-induced apoptotic retinal cell death was regulated by both NOS and Src activity. Because Src activity is pivotal in several CNS regions, the data presented herein highlight that Src modulation is a critical step in excitatory retinal cell death.