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1.
An. acad. bras. ciênc ; 90(1): 495-507, Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-886920

RESUMO

ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.


Assuntos
Floroglucinol/farmacologia , Criopreservação/métodos , Crioprotetores/farmacologia , Musa/citologia , Rizoma/citologia , Valores de Referência , Sacarose/farmacologia , Fatores de Tempo , Reprodutibilidade dos Testes , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/fisiologia , Musa/efeitos dos fármacos , Rizoma/efeitos dos fármacos , Glicerol/farmacologia
2.
An Acad Bras Cienc ; 90(1): 495-507, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29069135

RESUMO

The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.


Assuntos
Criopreservação/métodos , Crioprotetores/farmacologia , Musa/citologia , Floroglucinol/farmacologia , Rizoma/citologia , Glicerol/farmacologia , Musa/efeitos dos fármacos , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/fisiologia , Valores de Referência , Reprodutibilidade dos Testes , Rizoma/efeitos dos fármacos , Sacarose/farmacologia , Fatores de Tempo
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