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SUMMARY: This research aimed to create a multidimensional equation for predicting an optimal body composition model for elite-level European male volleyball players. The subject sample consisted of 36 elite volleyball players: national team members from two European countries - Slovenia and Serbia - in the 2017 season. Measurement of body composition was carried out using electrical multichannel bioimpedance (BIA - InBody 720), in which 11 variables were used (body height as a longitudinal one, and the other 10 as a system of body composition indicators). Based on the results of descriptive statistics, we can state that elite European male volleyball players are tall, heavy, and with nutritional status at the level of 198.5?6.5 cm, 92.3?5.9 kg and 23.44?1.44 kg•m-2, respectively. The percentage of body fat in the whole sample was 7.91?3.03 %, and the skeletal muscle mass index averaged 12.49?0.80 kg•m-2. For the evaluation and prediction of an optimal body composition model of elite male volleyball players, a multidimensional score (BC_Score) was defined, based on a simple, but sport-specific and playing position-sensitive model equation based on three dominant body characteristics of elite volleyball players: BH, PBF, and SMMI. In this manner, coaches can have a tool for managing the body composition status of players according to position, in terms of a deterministic, fully controlled system.
RESUMEN: Esta investigación tuvo como objetivo crear una ecuación multidimensional para predecir un modelo de composición corporal óptimo para los jugadores de voleibol masculinos europeos de élite. La muestra consistió en 36 jugadores de voleibol de élite, miembros del equipo nacional de dos países, Eslovenia y Serbia, en la temporada 2017. La composición corporal se realizó mediante bioimpedancia eléctrica multicanal (BIA - InBody 720), en la que se utilizaron 11 variables (la altura corporal como longitudinal y las otras 10 como sistema de indicadores de composición corporal). Con base en los resultados de las estadísticas descriptivas, observamos que los jugadores de voleibol de élite europeos son altos, pesados y con un estado nutricional de 198,5 ? 6,5 cm, 92,3 ? 5,9 kg y 23,44 ? 1,44 kg•m-2, respectivamente. El porcentaje de grasa corporal en toda la muestra fue de 7,91 ? 3,03 % y el índice de masa del músculo esquelético promedió 12,49 ? 0,80 kg•m-2. Para la evaluación y predicción de un modelo de composición corporal óptimo de jugadores de voleibol masculino de élite, se definió una puntuación multidimensional (BC_Score), basada en una ecuación de modelo simple, pero específica del deporte y sensible a la posición de juego, basada en tres características corporales dominantes de la élite. Jugadores de voleibol: BH, PBF y SMMI. De esta manera, los entrenadores pueden disponer de una herramienta para gestionar el estado de la composición corporal de los jugadores según la posición, en términos de un sistema determinista y totalmente controlado.
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Humanos , Masculino , Composição Corporal , Voleibol , Impedância Elétrica , PrevisõesRESUMO
In mid-June 2019, 3 months after cyclone Idai landfall in Mozambique, health authorities of Nhamatanda district reported an outbreak of Pellagra. Applying a mixed-method protocol, we carried out an investigation to characterize cases of pellagra, identify the associated factors for the outbreak using a case-control study, and explore the perceived impact on food security (availability, access, and usage) before and after Idai. We collected data from 121 cases and 121 controls and conducted in-depth interviews with 69 heads of households. The cases were more likely to be female (P < 0.01) and less educated (P < 0.01) than controls. Insufficient consumption of chicken and peanut before cyclone Idai arrival were statistically associated with pellagra (P < 0.05). From interviewed households' heads, 51% were experiencing food shortages even before the cyclone hit. Cyclone Idai served as a trigger to reduce niacin consumption below the threshold that protected Nhamatanda population from pellagra and caused a ≈2,300 case (707.9/100,000 inhabitants) outbreak.
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Tempestades Ciclônicas , Surtos de Doenças , Pelagra/epidemiologia , Pelagra/etiologia , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Moçambique/epidemiologia , Razão de Chances , Pele/patologiaRESUMO
The uncontrollable outbreak of the novel coronavirus (COVID-19) rapidly affected almost 230 countries across the world and territories since last year'2020 and its transmission mainly due to respiratory droplets. To fight and protect against micron dimension (~1.4 µm) corona virus the usage of disposable medical masks is one and only trivial option for patients, doctors, health employers and in fact mandatory for kids to senior citizens, as well as public places in a risky environment. Ordinary medical masks unable to self-sterilize in order to recycle for other appliances resulting further destroying impact of societies high economic and environmental costs. To minimize this global pandemic issue this proposal explores novel mechanism for further commercialization of surgical mask of photo-thermal and self-cleaning functionalization. Indeed, depositing few layer ultra-thin graphene coating onto low-melting temperature non-woven mask by tempering a dual mode laser induced mechanism. Incoming aqueous droplets are bounced off due the super-hydrophobic states were treated on the mask surface. Superficial hydrophobic surface yields an advanced safety towards approaching respiratory droplets. Due to the huge absorption coefficient capability of the sunrays activated laser-induced mask may rapidly boost temperature exceeds 85ºC under sunlight illumination, causes making the mask reusable after sunlight distillation. For SARS/coronavirus/ aerosolized bacteria, laser induced graphene mask is a recent breakthrough in superior antibacterial capacity. Furthermore, cost-effective and ultra-thin layered mask formulation recycled directly utilizes solar-driven desalination with remarkable self-exclusion performance for indelible usability. Featured review article, deals with remarkable achievements from forthcoming experimentation which may be inspired with layered mask designing by more progressive materials.
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This study presents an experimental approach to study the kinetics and fast release of volatile organic compounds (VOCs) upon reconstitution of instant coffee products. A sampling setup coupled to PTR-ToF-MS (Proton Transfer Reaction Time-of-Flight Mass Spectrometry) for the automated and reproducible reconstitution of instant coffee products was developed to monitor the dynamic release of VOCs. A rapid release of aroma compounds was observed in the first seconds upon hot water addition ("aroma burst"), followed by subsequent decrease in headspace (HS) intensities over the course of analysis. Differences in time-intensity release profiles of individual VOCs were correlated to their Henry's Law constant, vapor pressure and water solubility. The setup and approach proposed here have shown to be sensitive and to respond to fast dynamic changes in aroma release. It allows studying VOCs release upon reconstitution and supports the development of novel technologies and formulations for instant products with improved aroma release properties.
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Café/química , Análise de Alimentos/métodos , Odorantes/análise , Compostos Orgânicos Voláteis/análise , Análise de Alimentos/instrumentação , Cinética , Espectrometria de Massas/instrumentação , Espectrometria de Massas/métodos , Prótons , Solubilidade , ÁguaRESUMO
BACKGROUND: In January 2016, health authorities from Zambézia province, Mozambique reported the detection of some patients presenting with fever, arthralgia, and a positive result for chikungunya in an IgM-based Rapid Diagnostic Test (RDT). We initiated a study to investigate a potential chikungunya outbreak in the city of Quelimane. METHODS/PRINCIPAL FINDINGS: From February to June 2016, we conducted a cross-sectional study enrolling febrile patients attending five outpatient health units in Quelimane. Serum from each patient was tested for CHIKV and DENV, using IgM and IgG ELISA and qRT-PCR. Patients were also tested for malaria by RDT. Entomological surveys were performed around patients' households, and we calculated the proportion of positive ovitraps and the egg density per trap. A total of 163 patients were recruited, of which 99 (60.7%) were female. The median age was 28 years. IgM and IgG anti-CHIKV antibodies were identified in 17 (10.4%) and 103 (63.2%) patients, respectively. Plaque reduction neutralization assay confirmed the presence of anti-CHIKV antibodies in a subset of 11 tested patients with positive IgG results. IgM anti-DENV antibodies were found in 1 (0.9%) of 104 tested patients. Malaria was diagnosed in 35 (21.5%) patients, 2 of whom were also IgM-positive for CHIKV. Older age and lower education level were independently associated with the prevalence of IgG anti-CHIKV antibodies. Immature forms of Aedes aegypti were collected in 16 (20.3%) of 79 surveyed households. We also found that 25.0% (16/64) of the traps were positive, with an average of 90.8 eggs per pallet. CONCLUSIONS: Our investigation demonstrated that no CHIKV outbreak was ongoing in Quelimane; rather, endemic transmission of the virus has been ongoing. Aedes aegypti mosquitoes are abundant, but dengue cases occurred only sporadically. Further population-based cohort studies are needed to improve our understanding of aspects related to the dynamics of arboviral transmission in Mozambique, as well as in other parts of Sub-Saharan Africa.
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Febre de Chikungunya/epidemiologia , Dengue/epidemiologia , Surtos de Doenças , Adolescente , Adulto , Febre de Chikungunya/transmissão , Dengue/transmissão , Feminino , Humanos , Masculino , Moçambique/epidemiologia , Adulto JovemRESUMO
Background: In January 2016, health authorities from Zambézia province, Mozambique reported the detection of some patients presenting with fever, arthralgia, and a positive result for chikungunya in an IgM-based Rapid Diagnostic Test (RDT). We initiated a study to investigate a potential chikungunya outbreak in the city of Quelimane. Methods/principal findings: From February to June 2016, we conducted a cross-sectional study enrolling febrile patients attending five outpatient health units in Quelimane. Serum from each patient was tested for CHIKV and DENV, using IgM and IgG ELISA and qRT-PCR. Patients were also tested for malaria by RDT. Entomological surveys were performed around patients' households, and we calculated the proportion of positive ovitraps and the egg density per trap. A total of 163 patients were recruited, of which 99 (60.7%) were female. The median age was 28 years. IgM and IgG anti-CHIKV antibodies were identified in 17 (10.4%) and 103 (63.2%) patients, respectively. Plaque reduction neutralization assay confirmed the presence of anti-CHIKV antibodies in a subset of 11 tested patients with positive IgG results. IgM anti-DENV antibodies were found in 1 (0.9%) of 104 tested patients. Malaria was diagnosed in 35 (21.5%) patients, 2 of whom were also IgM-positive for CHIKV. Older age and lower education level were independently associated with the prevalence of IgG anti-CHIKV antibodies. Immature forms of Aedes aegypti were collected in 16 (20.3%) of 79 surveyed households. We also found that 25.0% (16/64) of the traps were positive, with an average of 90.8 eggs per pallet. Conclusions: Our investigation demonstrated that no CHIKV outbreak was ongoing in Quelimane; rather, endemic transmission of the virus has been ongoing. Aedes aegypti mosquitoes are abundant, but dengue cases occurred only sporadically. Further population-based cohort studies are needed to improve our understanding of aspects related to the dynamics of arboviral transmission in Mozambique, as well as in other parts of Sub-Saharan Africa.
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Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Surtos de Doenças , Dengue/epidemiologia , Febre de Chikungunya/epidemiologia , Dengue/transmissão , Febre de Chikungunya/transmissão , Moçambique/epidemiologiaRESUMO
An anthropometric analysis was conducted on 64 competitive young male road cyclists of different age categories (U17; U19; & U23 years of age). The purpose of the study is to find asymmetries between the left and right side of upper and lower limbs with the NX-16 (TC2) 3D body scanner, which includes measurement of left and right upper arm girth, elbow girth, forearm girth, wrist girth, thigh girth, knee girth, thigh length, calf girth, and shin length. Body composition was measured by the bioelectrical impedance machine InBody 720 (Biospace Ltd.). Results of body composition measurements of male road cyclists showed that U17 and U19 youth road cyclist differed statistically in five (from 11) paired variables, and the U23 age group differed statistically in six (from 11) paired variables. All of the age groups differed statistically in elbow, forearm, and calf girth. The main finding of study was that as the age of a cyclist increases, there is a tendency to increase asymmetries between the left and right side of several body segments.
Se realizó un análisis antropométrico de 64 ciclistas hombres, competidores de carretera, de diferentes categorías de edad (M17 y M23 años). El objetivo del estudio fue encontrar asimetrías entre el lado izquierdo y el lado derecho de los miembros superiores e inferiores con escáner corporal NX-16 (TC2) 3D, que incluyó la medición de las circunferencias izquierda y derecha de la parte superior del brazo, circunferencia del codo, circunferencia del antebrazo, y de la muñeca, circunferencia del muslo, de la cintura y de la rodilla; se midió el largo del muslo, circunferencia de la pantorrilla y el largo de la tibia. La composición corporal se midió mediante la máquina de impedancia bioeléctrica InBody 720 (Biospace Ltd.). Los resultados de las mediciones de la composición corporal de los ciclistas de carretera mostraron que los ciclistas juveniles de las categorías M17 y M19 difirían estadísticamente en cinco (de 11) variables pareadas, y el grupo de edad de los 23 años difirió estadísticamente en seis variables. Todos los grupos etarios difirieron estadísticamente en el codo, el antebrazo y en la circunferencia de la pantorrilla. El principal hallazgo del estudio indicó que al aumentar de edad un ciclista, se observa un aumento de la asimetría entre los lados izquierdo y derecho de varios segmentos corporales.
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Humanos , Masculino , Adolescente , Adulto Jovem , Antropometria , Ciclismo , Extremidades/anatomia & histologia , Distribuição por Idade , Composição CorporalRESUMO
Background: In Mozambique, the majority of rabies outbreaks are unreported and data on the epidemiological features of human rabies and animal bites are scarce. An outbreak of human rabies in adjacent Maputo and Matola cities in 2014 prompted us to investigate the epidemiology, clinical features and risk factors of human rabies and animal bites in the two cities. Methodology/principal findings: We reviewed cases of human rabies and animal bites from April to July 2014, and carried out a community investigation in July and August in the neighborhoods where cases of human rabies resided. This investigation included collection of clinical, demographic and epidemiological information and a case control study to investigate the risk factors associated with human rabies. Fourteen cases of human rabies were detected in Maputo (n = 10) and Matola (n = 3) cities and neighbouring Boane district (n = 1) between April and August 2014, all of whom had been admitted to hospital. All had a recent history of dog bite. Of the 14 rabid dogs, only one had been immunized. 819 cases of animal bites were registered, of which 64.6% (529/819) were from Maputo City. Dogs were responsible for 97.8% (801/819) of all animal bites, but only 27.0% (126/467) were immunized. Factors significantly associated with human rabies were: age <15 years (p = 0.05), bite by stray dog (p = 0.002), deep wound (p = 0.02), bite in the head (p = 0.001), bite by unimmunized dog (p = 0.01), no use of soap and water (p = 0.001), and no post-exposure prophylaxis (p = 0.01). Conclusions/significance: Implementation of control measures for rabies is poor in Maputo and Matola cities, where cases of human rabies were strongly associated with bites by stray and unvaccinated dogs and irregular implementation of post-exposure measures...
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Humanos , Animais , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto , Raiva/mortalidade , Raiva/prevenção & controle , Surtos de Doenças/prevenção & controle , Profilaxia Pós-Exposição/métodos , Hepatite Infecciosa Canina/prevenção & controle , Mordeduras e Picadas de Insetos/epidemiologia , Mordeduras e Picadas/prevenção & controle , Fatores de Risco , MoçambiqueRESUMO
Monitoring prevalence of advanced human immunodeficiency virus (HIV) disease (i.e., CD4+ T-cell count <200 cells/µL) among persons starting antiretroviral therapy (ART) is important to understand ART program outcomes, inform HIV prevention strategy, and forecast need for adjunctive therapies.*,,§ To assess trends in prevalence of advanced disease at ART initiation in 10 high-burden countries during 2004-2015, records of 694,138 ART enrollees aged ≥15 years from 797 ART facilities were analyzed. Availability of national electronic medical record systems allowed up-to-date evaluation of trends in Haiti (2004-2015), Mozambique (2004-2014), and Namibia (2004-2012), where prevalence of advanced disease at ART initiation declined from 75% to 34% (p<0.001), 73% to 37% (p<0.001), and 80% to 41% (p<0.001), respectively. Significant declines in prevalence of advanced disease during 2004-2011 were observed in Nigeria, Swaziland, Uganda, Vietnam, and Zimbabwe. The encouraging declines in prevalence of advanced disease at ART enrollment are likely due to scale-up of testing and treatment services and ART-eligibility guidelines encouraging earlier ART initiation. However, in 2015, approximately a third of new ART patients still initiated ART with advanced HIV disease. To reduce prevalence of advanced disease at ART initiation, adoption of World Health Organization (WHO)-recommended "treat-all" guidelines and strategies to facilitate earlier HIV testing and treatment are needed to reduce HIV-related mortality and HIV incidence.
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Antirretrovirais/uso terapêutico , Infecções por HIV/tratamento farmacológico , Infecções por HIV/epidemiologia , África/epidemiologia , Contagem de Linfócito CD4/estatística & dados numéricos , Infecções por HIV/imunologia , Haiti/epidemiologia , Humanos , Prevalência , Vietnã/epidemiologiaRESUMO
Although Kokernot et al. found neutralizing antibodies against ZIKV in Mozambique in 1957 [20], the country has repeatedly been excluded from the list of countries with a past history of ZIKV. The current Centers for Disease Control and Prevention map, the recent World Health Organization Risk Assessment map on ZIKV in the Africa region, and most of the recent literature mapping countries with a current and past history of ZIKV have consistently excluded Mozambique [10, 21, 22] from the list of countries with past serological evidence of ZIKV. This might result in errors in the calculation and interpretation of the risk of Zika in Mozambique as well as in the region. In this regard, in this manuscript we revisit findings of the study conducted by Kokernot et al. in an attempt to discuss the current risk of Zika in the country. The survey, conducted in 1957, was published in Portuguese in 1960 [20]. This study was part of a larger study on arboviruses, in which blood samples were screened for antibodies against 13 arboviruses, including ZIKV, chikungunya, Rift Valley fever, Sindbis, Middleburg, and Wesselsbron. Samples were collected in 29 localities situated widely apart from each other throughout the country between July and August 1957. In each locality, they selected an average of 30 local residents who had been born in the area with no history of travel outside in their lifetime. The samples were analyzed in South Africa, using confirmatory neutralization testing (NT). NT was performed using an in vivo system. For this purpose, previously titrated virus strains for each arbovirus being tested were incubated with each participant's serum and inoculated into Swiss mice to assess the neutralization profile of each serum against each virus strain. For ZIKV, the prototype ZIKV strain was used [18], and both adult and newborn mice were used for inoculation. An amount of 0.03 mL of the preparation virus and serum was inoculated intracerebrally, as previously described [18], and each mouse was observed daily between 10 17 days to assess the viral effect. The authors found neutralizing antibodies to all of the viruses and concluded that the whole length of Mozambique was a "tropical corridor" of arbovirus activity. The viruses with highest prevalence rates of neutralizing antibodies were chikungunya (21.0%), Wesselsbron (15.9%), Bunyamwera (24.1%), Pongola (23.2%) and Bwamba fever (24.7%)...
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Humanos , Animais , Masculino , Feminino , Controle de Mosquitos/história , Infecção por Zika virus/epidemiologia , Mosquitos Vetores , Infecção por Zika virus/história , Moçambique/epidemiologiaRESUMO
Background: In Sub-Saharan Africa, including Mozambique, acute bacterial meningitis (ABM) represents a main cause of childhood mortality. The burden of ABM is seriously underestimated because of the poor performance of culture sampling, the primary method of ABM surveillance in the region. Low quality cerebrospinal fluid (CSF) samples and frequent consumption of antibiotics prior to sample collection lead to a high rate of false-negative results. To our knowledge, this study is the first to determine the frequency of ABM in Mozambique using real-time polymerase chain reaction (qPCR) and to compare results to those of culture sampling. Method: Between March 2013 and March 2014, CSF samples were collected at 3 regional hospitals from patients under 5 years of age, who met World Health Organization case definition criteria for ABM. Macroscopic examination, cytochemical study, culture, and qPCR were performed on all samples. Results: A total of 369 CSF samples were collected from children clinically suspected of ABM. qPCR showed a significantly higher detection rate of ABM-causing pathogens when compared to culture (52.3% [193/369] versus 7.3% [27/369], p = 0.000). The frequency of Streptococcus pneumoniae, Haemophilus influenzae, group B Streptococci, and Neisseria meningitidis were 32.8% (121/369), 12.2%, (45/369), 3.0% (16/369) and 4.3% (11/369), respectively, significantly higher compared to that obtained on culture (p < 0.001 for each). Conclusion: Our findings demonstrate that culture is less effective for the diagnosis of ABM than qPCR. The common use of culture rather than qPCR to identify ABM results in serious underestimation of the burden of the disease, and our findings strongly suggest that qPCR should be incorporated into surveillance activities for ABM. In addition, our data showed that S. pneumoniae represents the most common cause of ABM in children under 5 years of age.
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Humanos , Masculino , Feminino , Recém-Nascido , Pré-Escolar , DNA Bacteriano/líquido cefalorraquidiano , Meningites Bacterianas/microbiologia , Reação em Cadeia da Polimerase em Tempo Real/métodos , Estações do Ano , Streptococcus agalactiae/isolamento & purificação , Streptococcus agalactiae/efeitos dos fármacos , Streptococcus agalactiae/genética , Streptococcus pneumoniae/isolamento & purificação , Streptococcus pneumoniae/efeitos dos fármacos , Streptococcus pneumoniae/genética , Índice de Gravidade de Doença , DNA Bacteriano/genética , Testes de Sensibilidade Microbiana , Haemophilus influenzae/isolamento & purificação , Haemophilus influenzae/efeitos dos fármacos , Haemophilus influenzae/genética , Análise Multivariada , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/epidemiologia , Farmacorresistência Bacteriana , Moçambique/epidemiologia , Neisseria meningitidis/isolamento & purificação , Neisseria meningitidis/efeitos dos fármacos , Neisseria meningitidis/genéticaRESUMO
The Beijing genotype is a lineage of Mycobacterium tuberculosis that is distributed worldwide and responsible for large epidemics, associated with multidrug-resistance. However, its distribution in Africa is less understood due to the lack of data. Our aim was to investigate the prevalence and possible transmission of Beijing strains in Mozambique by a multivariate analysis of genotypic, geographic and demographic data. A total of 543 M. tuberculosis isolates from Mozambique were spoligotyped. Of these, 33 were of the Beijing lineage. The genetic relationship between the Beijing isolates were studied by identification of genomic deletions within some Regions of Difference (RD), Restriction Fragment Length Polymorphism (RFLP) and Mycobacterial Interspersed Repetivie Unit variable number tandem repeat (MIRU-VNTR). Beijing strains from South Africa, representing different sublineages were included as reference strains. The association between Beijing genotype, Human Immunodeficiency Virus (HIV) serology and baseline demographic data was investigated. HIV positive serostatus was significantly (p=0.023) more common in patients with Beijing strains than in patients with non-Beijing strains in a multivariable analysis adjusted for age, sex and province (14 (10.9%) of the 129 HIV positive patients had Beijing strains while 6/141 (4.3%) of HIV negative patients had Beijing strains). The majority of Beijing strains were found in the Southern region of Mozambique, particularly in Maputo City (17%). Only one Beijing strain was drug resistant (multi-drug resistant). By combined use of RD and spoligotyping, three genetic sublineages could be tentatively identified where a distinct group of four isolates had deletion of RD150, a signature of the "sublineage 7" recently emerging in South Africa. The same group was very similar to South African "sublineage 7" by RFLP and MIRU-VNTR, suggesting that this sublineage could have been recently introduced in Mozambique from South Africa, in association with HIV infection.
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Humanos , Masculino , Feminino , Tuberculose Pulmonar/microbiologia , Polimorfismo de Fragmento de Restrição , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Coinfecção/microbiologia , Mycobacterium tuberculosis , Mycobacterium tuberculosis/genética , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Tuberculose Resistente a Múltiplos Medicamentos/genética , Coinfecção/epidemiologia , MoçambiqueRESUMO
BACKGROUND: Human T-Cell Lymphotropic Virus Type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). It has been estimated that 10-20 million people are infected worldwide, but no successful treatment is available. Recently, the epidemiology of this virus was addressed in blood donors from Maputo, showing rates from 0.9 to 1.2%. However, the origin and impact of HTLV endemic in this population is unknown. OBJECTIVE: To assess the HTLV-1 molecular epidemiology in Mozambique and to investigate their relationship with HTLV-1 lineages circulating worldwide. METHODS: Blood donors and HIV patients were screened for HTLV antibodies by using enzyme immunoassay, followed by Western Blot. PCR and sequencing of HTLV-1 LTR region were applied and genetic HTLV-1 subtypes were assigned by the neighbor-joining method. The mean genetic distance of Mozambican HTLV-1 lineages among the genetic clusters were determined. Human mitochondrial (mt) DNA analysis was performed and individuals classified in mtDNA haplogroups. RESULTS: LTR HTLV-1 analysis demonstrated that all isolates belong to the Transcontinental subgroup of the Cosmopolitan subtype. Mozambican HTLV-1 sequences had a high inter-strain genetic distance, reflecting in three major clusters. One cluster is associated with the South Africa sequences, one is related with Middle East and India strains and the third is a specific Mozambican cluster. Interestingly, 83.3% of HIV/HTLV-1 co-infection was observed in the Mozambican cluster. The human mtDNA haplotypes revealed that all belong to the African macrohaplogroup L with frequencies representatives of the country. CONCLUSIONS: The Mozambican HTLV-1 genetic diversity detected in this study reveals that although the strains belong to the most prevalent and worldwide distributed Transcontinental subgroup of the Cosmopolitan subtype, there is a high HTLV diversity that could be correlated with at least 3 different HTLV-1 introductions in the country. The significant rate of HTLV-1a/HIV-1C co-infection, particularly in the Mozambican cluster, has important implications for the controls programs of both viruses.
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Infecções por HTLV-I/epidemiologia , Infecções por HTLV-I/virologia , Vírus Linfotrópico T Tipo 1 Humano/classificação , Vírus Linfotrópico T Tipo 1 Humano/genética , Doadores de Sangue , Western Blotting , Análise por Conglomerados , Genótipo , Infecções por HIV/complicações , Vírus Linfotrópico T Tipo 1 Humano/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas , Epidemiologia Molecular , Dados de Sequência Molecular , Moçambique/epidemiologia , Filogenia , Reação em Cadeia da Polimerase , RNA Viral/genética , Análise de Sequência de DNARESUMO
Introdução: O espaço perineural é reconhecido como rota de propagação do carcinoma espinocelular (CEC) e, quando acometido, tem sido relacionado com pior prognóstico, maior recorrência e menor tempo de sobrevivência. Contudo, seu papel nos tumores precoces de cavidade oral ainda não está totalmente esclarecido. Objetivos: comparar a sobrevivência global e livre de doença entre pacientes com carcinoma espinocelular precoce de língua oral e soalho de boca com e sem invasão perineural; comparar a ocorrência de fatores clínicos e anátomo-patológicos entre os grupos; e descrever os casos de óbito para análise crítica da realização do tratamento radioterápico adjuvante. Métodos: Foi realizado estudo retrospectivo longitudinal de caso-controle, de 42 pacientes operados por CEC precoce (T1/T2 N0) de língua oral e soalho de boca, divididos em dois grupos: com invasão perineural (n=16) e sem invasão (n=26). Variáveis clínicas, anátomo-patológicas e de seguimento, incluindo realização de tratamento adjuvante foram comparadas. Resultados: No grupo com invasão perineural houve maior freqüência de homens (p=0,008), óbito relacionado (p=0,015), quantidade de cigarro consumida (p=0,042) e menor freqüência de não etilistas. As médias do maior diâmetro (p=0,046) e da espessura (p=0,002) do CEC primário foram maiores nesse grupo. Conclusões: Houve menor sobrevivência global no grupo de doentes com CEC precoce de língua oral e soalho com invasão perineural (p=0,019), apesar da maior freqüência de radioterapia adjuvante. Todos os óbitos relacionados ocorreram no grupo com invasão perineural, por recidiva local, sendo que, em dois casos, nenhuma outra característica de mau prognóstico foi observada. À análise multivariada, a presença de invasão perineural foi indicada como fator associado ao óbito correlacionado, de maneira não significante (p=0,275). Os resultados favorecem a indicação de tratamento radioterápico adjuvante.
Introduction: perineural space is known as a pathway for spreading of the squamous cell carcinoma (SCC). Its invasion has been associated with worse prognosis, greater rates of recurrence and lower survival rates. However, its impact on the initial oral cavity tumors is not well established. Objectives: to compare global survival and disease free survival between patients with initial SCC of tongue and floor of mouth, with and without perineural invasion; to compare clinical and pathological features between the two groups; and to describe the cases of death for a critical analysis of the use of adjuvant radiotherapy. Methods: retrospective longitudinal case-control study of 42 patients surgically treated for initial SCC (T1/T2 N0 ? UICC-2002) of oral tongue and floor of mouth. The patients were divided into two groups: with (n=16) and without (n=26) perineural invasion. Clinical and pathological features, as well as the follow-up and treatment information were compared. Results: the group with perineural invasion presented a higher frequency of male patients (p=0.008) and of tobacco use (p=0.042) and lower frequency of non users of alcohol. The mean of greater tumor diameter (p=0.046) and thickness (p=0,002) were higher in this group. Those patients also had higher disease specific death (p=0.015) and a lower survival (p=0.019), regardless of a higher frequency of adjuvant treatment with radiotherapy. All disease specific deaths occurred in this group and, in two of these cases, no other known features of worse prognosis were observed. At the multivariate analysis, the perineural invasion was related to disease-specific death in a non significant manner (p=0.275). Conclusion: These results point out the perineural invasion as a feature of worse prognosis, even in initial tumors and despite more aggressive treatment.
RESUMO
The effect of the addition of (2,6-O-dimethyl)-beta-cyclodextrin (Me beta CD) during growth of Bordetella pertussis in synthetic Stainer-Scholte liquid medium (SS) on lipopolysaccharide (LPS; endotoxin) release was investigated. The Me beta CD concentration used (3 mg/ml) was chosen according to the optimal level found in previous studies to enhance major soluble antigen production. The profiles in SDS-PAGE (sodium dodecyl sulphate/polyacrylamide gel electrophoresis) of LPS extracted from cells grown in SS and SS + Me beta CD media revealed similar patterns. Although the LPS content of whole cells decreased during cell growth, yields obtained at different growth periods in cyclodextrin medium were lower than those corresponding to SS medium alone. Consequently, the level of LPS released in supernatants of both media increased during cellular growth. This amount of free LPS was higher in the cyclodextrin liquid medium and became significant at the beginning of the stationary growth phase. Binding of cyclodextrin to pertussis cells could account for the data obtained. Similar results were obtained with all species of the genus Bordetella.
Assuntos
Bordetella pertussis/crescimento & desenvolvimento , Ciclodextrinas/farmacologia , Lipopolissacarídeos/análise , Proteínas da Membrana Bacteriana Externa/análise , Bordetella pertussis/efeitos dos fármacos , Bordetella pertussis/metabolismo , Meios de Cultura , Eletroforese em Gel de Poliacrilamida , Técnicas In VitroRESUMO
The production of Bordetella pertussis extracytoplasmic filamentous haemagglutinin (FHA) and pertussis toxin (PT) in a bioreactor under stirring conditions was studied in order to investigate the effect of hydromechanical forces on yields of both antigens. It was shown that FHA loses its haemagglutinin activity when the power transmitted by the agitator and the aerator per unit volume increases, whereas PT production is not affected. The loss of FHA activity can be explained by the action of shear forces on the filamentous structure of this antigen.