RESUMO
Introducción: la resistencia antimicrobiana constituye uno de los mayores problemas que afronta la salud pública mundial. La aparición de cepas resistentes no solo de origen clínico sino también ambiental agrava la situación. Entre los microorganismos que presentan esta característica se destaca la especie bacteriana Escherichia coli debido a su doble papel como indicador de contaminación fecal y como patógeno. Objetivos: aislar e identificar hasta especie aislamientos de Escherichia coli a partir de muestras de agua procedentes de ríos contaminados de La Habana y determinar la susceptibilidad antimicrobiana in vitro de estos aislados. Métodos: se estudiaron 113 aislamientos de bacterias coliformes aislados de 10 estaciones de muestreo ubicadas en la zona urbana de los ríos capitalinos Almendares, Quibú y Luyanó en el período comprendido de febrero de 2008 hasta junio de 2010. La identificación de los aislados, la determinación de la susceptibilidad antimicrobiana y la búsqueda de b-lactamasas de espectro extendido se realizó mediante el método automatizado VITEK. Resultados: se identificaron 113 cepas ambientales de Escherichia coli. Se demostró que 23 por ciento de los aislamientos resultaron resistentes al menos a uno de los antimicrobianos evaluados. Los mayores porcentajes de resistencia se observaron frente a ampicilina, sulfametoxazol-trimetropin y ciprofloxacina. Conclusiones: la presencia de aislados de E. coli con multirresistencia antimicrobiana en estos ríos indica claramente el riesgo biológico que implica el uso de sus aguas(AU)
Introduction: antimicrobial resistance is one of the biggest problems facing global public health. The emergence of resistant clinical and environmental strains worsens the situation. Among the microorganisms with antimicrobial resistance, Escherichia coli species stands out due to its dual role as fecal contamination indicator and pathogen. Objectives: to isolate and identify Escherichia coli isolates from water samples from polluted rivers located in La Habana, and to determine their antimicrobial in vitro susceptibility. Methods: one hundred thirteen isolates of coliform bacteria isolated from 10 sampling stations in the capital´s urban areas near Almendares, Quibú and Luyanó rivers were studied in the period of February 2008 to June 2010. The identification of isolates, the determination of antimicrobial susceptibility and the search for extended-spectrum b-lactamase were all performed using VITEK automated method. Results: one hundred thirteen environmental strains of Escherichia coli were identified. It showed that 23 percent of the isolates were resistant to at least one of the tested antimicrobials. The highest percentages of resistance were observed to ampicilline, sulfamethoxazole-trimethoprim and ciprofloxacin. Conclusions: the presence of E. coli isolates with multiple antimicrobial resistances in these rivers clearly indicates the biological risk involving the use of their waters(AU)
Assuntos
Escherichia coli , Água Doce/microbiologia , Ecossistema , Testes de Sensibilidade Microbiana , Rios , Microbiologia da ÁguaRESUMO
Introducción: la resistencia antimicrobiana constituye uno de los mayores problemas que afronta la salud pública mundial. La aparición de cepas resistentes no solo de origen clínico sino también ambiental agrava la situación. Entre los microorganismos que presentan esta característica se destaca la especie bacteriana Escherichia coli debido a su doble papel como indicador de contaminación fecal y como patógeno. Objetivos: aislar e identificar hasta especie aislamientos de Escherichia coli a partir de muestras de agua procedentes de ríos contaminados de La Habana y determinar la susceptibilidad antimicrobiana in vitro de estos aislados. Métodos: se estudiaron 113 aislamientos de bacterias coliformes aislados de 10 estaciones de muestreo ubicadas en la zona urbana de los ríos capitalinos Almendares, Quibú y Luyanó en el período comprendido de febrero de 2008 hasta junio de 2010. La identificación de los aislados, la determinación de la susceptibilidad antimicrobiana y la búsqueda de b-lactamasas de espectro extendido se realizó mediante el método automatizado VITEK. Resultados: se identificaron 113 cepas ambientales de Escherichia coli. Se demostró que 23 % de los aislamientos resultaron resistentes al menos a uno de los antimicrobianos evaluados. Los mayores porcentajes de resistencia se observaron frente a ampicilina, sulfametoxazol-trimetropin y ciprofloxacina. Conclusiones: la presencia de aislados de E. coli con multirresistencia antimicrobiana en estos ríos indica claramente el riesgo biológico que implica el uso de sus aguas.
Introduction: antimicrobial resistance is one of the biggest problems facing global public health. The emergence of resistant clinical and environmental strains worsens the situation. Among the microorganisms with antimicrobial resistance, Escherichia coli species stands out due to its dual role as fecal contamination indicator and pathogen. Objectives: to isolate and identify Escherichia coli isolates from water samples from polluted rivers located in La Habana, and to determine their antimicrobial in vitro susceptibility. Methods: one hundred thirteen isolates of coliform bacteria isolated from 10 sampling stations in the capital´s urban areas near Almendares, Quibú and Luyanó rivers were studied in the period of February 2008 to June 2010. The identification of isolates, the determination of antimicrobial susceptibility and the search for extended-spectrum b-lactamase were all performed using VITEK automated method. Results: one hundred thirteen environmental strains of Escherichia coli were identified. It showed that 23 % of the isolates were resistant to at least one of the tested antimicrobials. The highest percentages of resistance were observed to ampicilline, sulfamethoxazole-trimethoprim and ciprofloxacin. Conclusions: the presence of E. coli isolates with multiple antimicrobial resistances in these rivers clearly indicates the biological risk involving the use of their waters.
Assuntos
Escherichia coli/efeitos dos fármacos , Água Doce/microbiologia , Ecossistema , Testes de Sensibilidade Microbiana , Rios , Microbiologia da ÁguaRESUMO
INTRODUCTION: Antimicrobial resistance is one of the biggest problems facing global public health. The emergence of resistant clinical and environmental strains worsens the situation. Among the microorganisms with antimicrobial resistance, Escherichia coil species stands out due to its dual role as fecal contamination indicator and pathogen. OBJECTIVES: To isolate and identify Escherichia coil isolates from water samples from polluted rivers located in La Habana, and to determine their antimicrobial in vitro susceptibility. METHODS: One hundred thirteen isolates of coliform bacteria isolated from 10 sampling stations in the capital's urban areas near Almendares, Quibú and Luyanó rivers were studied in the period of February 2008 to June 2010. The identification of isolates, the determination of antimicrobial susceptibility and the search for extended-spectrum beta-lactamase were all performed using VITEK automated method. RESULTS: One hundred thirteen environmental strains of Escherichia coli were identified. It showed that 23% of the isolates were resistant to at least one of the tested antimicrobials. The highest percentages of resistance were observed to ampicilline, sulfamethoxazole-trimethoprim and ciprofloxacin. CONCLUSIONS: The presence of E. coil isolates with multiple antimicrobial resistances in these rivers clearly indicates the biological risk involving the use of their waters.