RESUMO
Com o reconhecimento do exercício da Odontologia Hospitalar e sua aprovação como uma nova especialidade pelo Conselho Federal de Odontologia em 2023, e validação das novas Diretrizes Curriculares Nacionais para o Curso de Odontologia homologadas em 2021, os cursos de graduação em Odontologia no Brasil tendem a qualificar futuros profissionais em formação para atuar em ambiente hospitalar, de forma sistematizada. Isso visa propiciar aosestudantes uma vivência interprofissional inerente aos hospitais, favorecendo a interação positiva entre várias áreas da saúde. Este estudo descritivo tem como objetivo relatar a experiência no processo de aprendizagem colaborativa entre profissionais de saúde residentes do Programa de Residência Multiprofissional em Saúde do Idoso, na área profissional de Odontologia, de um Hospital Universitário de Curitiba e estudantes de graduação do curso de Odontologia de uma instituição de ensino superior. Durante odesenvolvimento de diferentes atividades acadêmicas, observou-se que a utilização desta metodologia de ensino-aprendizagem caracterizou-se como uma ferramenta importante, buscando fortalecer as trocas de vivências práticas, propiciando discussões entre estudantes e profissionais residentes, por meio da mediação dos docentes, os quais buscavam sempre incentivar a construção do conhecimento crítico reflexivo, oportunizando uma experiência de formação discente interprofissional ampliada (AU).
Con el reconocimiento de la práctica de la Odontología Hospitalaria y su aprobación como nueva especialidad por el Consejo Federal de Odontología en 2023, y la validación de las nuevas Directrices Curriculares Nacionales para la Carrera de Odontología aprobadas en 2021, las carreras de pregrado en Odontología en Brasil tienden a capacitar a los futuros profesionales en formación para trabajar en el entorno hospitalario, de forma sistemática. Se pretende proporcionar a los estudiantes una experienciainterprofesional inherente a los hospitales, favoreciendo la interacción positiva entre las diversas áreas de la salud. Este estudio descriptivo tiene como objetivo relatar la experiencia en el proceso de aprendizaje colaborativo entre profesionales de lasalud residentes en el Programa de Residencia Multidisciplinaria en Salud del Adulto Mayor, en el área profesional de Odontología, en un Hospital Universitario de Curitiba y estudiantes de pregrado de la carrera de Odontología en Curitiba de una institución de educación superior. Durante el desarrollo de diferentes actividades académicas, se observó que el uso de esta metodología de enseñanza-aprendizaje se caracterizó como una herramienta importante, buscando fortalecer el intercambio de experiencias prácticas, promoviendo el debate entre estudiantes y profesionales residentes, a través de la mediación de los docentes. que siempre buscó incentivar la construcción de conocimientos reflexivos críticos, brindando una experiencia ampliada de formación interprofesional de los estudiantes (AU).
With the recognition of the Hospital Dentistry practice,its approval as a new specialty by the Federal Dental Council in 2023 and validation of the new National Curricular Guidelines for the Dentistry Course approved in 2021, undergraduate Dentistry courses in Brazil tend to qualify future professionals in training to work in a hospital environment, in a systematic manner. This aims to provide students with an interprofessional experience inherent to hospitals, favoring the positive interaction between various health specialties. This descriptive study reportsthe experience in the collaborative learning process between health professionals residentsof the Multiprofessional Residency Program in Elderly Health in the professional area of Dentistry at a University Hospital in Curitiba,and undergraduate Dentistry students from a higher education institutionin Curitiba. During the development of different academic activities, it was observed that the use of this teaching-learning methodology was characterized as an important tool, seeking to strengthen the exchange of practical experiences, promoting discussions betweenstudents and resident professionals, by the mediation of professors, who always encourage the construction of critical reflective knowledge, providing an expanded interprofessional student training experience (AU).
Assuntos
Humanos , Masculino , Feminino , Aprendizagem Baseada em Problemas , Equipe Hospitalar de Odontologia , Práticas Interdisciplinares/métodos , Educação InterprofissionalRESUMO
The use of cocaine and its main derivative, crack, can cause some systemic effects that may lead to the development of some oral disorders. To assess the oral health of people with a crack cocaine use disorder and identify salivary protein candidates for biomarkers of oral disorders. A total of 40 volunteers hospitalized for rehabilitation for crack cocaine addiction were enrolled; nine were randomly selected for proteomic analysis. Intraoral examination, report of DMFT, gingival and plaque index, xerostomia, and non-stimulated saliva collection were performed. A list of proteins identified was generated from the UniProt database and manually revised. The mean age (n=40) was 32 (±8.88; 18-51) years; the mean DMFT index was 16±7.70; the mean plaque and gingival index were 2.07±0.65 and 2.12±0.64, respectively; and 20 (50%) volunteers reported xerostomia. We identified 305 salivary proteins (n=9), of which 23 were classified as candidate for biomarkers associated with 14 oral disorders. The highest number of candidates for biomarkers was associated with carcinoma of head and neck (n=7) and nasopharyngeal carcinoma (n=7), followed by periodontitis (n=6). People with a crack cocaine use disorder had an increased risk of dental caries and gingival inflammation; less than half had oral mucosal alterations, and half experienced xerostomia. As possible biomarkers for 14 oral disorders, 23 salivary proteins were identified. Oral cancer and periodontal disease were the most often associated disorders with biomarkers.
Assuntos
Cocaína Crack , Cárie Dentária , Xerostomia , Humanos , Cocaína Crack/efeitos adversos , Cocaína Crack/metabolismo , Proteômica , Xerostomia/induzido quimicamente , Xerostomia/metabolismo , Saliva/metabolismo , Proteínas e Peptídeos SalivaresRESUMO
BACKGROUND: Nosocomial pneumonia ranks among the top 5 diseases that lead to additional financial costs due to hospitalization. This study aimed to evaluate the cost of oral care and its clinical effectiveness in preventing pneumonia in a systematic review. METHODS: The search was conducted in the following databases: PubMed, Cochrane Library, Web of Sciences, Scopus, CINAHL, LILACS, complemented by gray literature and manual search, between January/2021 and August/2022. Two independent reviewers extracted data from the selected articles, individually analyzing each study's quality using the BMJ Drummond checklist. The data were tabulated by clinical or economic type. RESULTS: A total of 3,130 articles were identified; the eligibility criteria were verified, and 12 articles were selected for qualitative analysis. Only 2 achieved satisfactory quality assessment for economic analysis studies. There was heterogeneity between clinical and economic data. Eleven of the 12 studies reported a decrease in the incidence of nosocomial pneumonia following the application of oral care practices. Most authors reported a reduction in the estimate of individual costs, followed by a decrease in the need for antibiotic therapy. The costs of oral care were very low compared to other costs. CONCLUSIONS: Despite the low level of evidence in the literature, heterogeneity and poor quality of the selected studies, most studies concluded that oral care seemed to lead to reduced costs in hospital expenses for treating pneumonia.
Assuntos
Infecção Hospitalar , Pneumonia Associada a Assistência à Saúde , Pneumonia , Humanos , Infecção Hospitalar/prevenção & controle , Pneumonia Associada a Assistência à Saúde/prevenção & controle , Pneumonia/prevenção & controle , Antibacterianos/uso terapêutico , Resultado do TratamentoRESUMO
Abstract The use of cocaine and its main derivative, crack, can cause some systemic effects that may lead to the development of some oral disorders. Objective To assess the oral health of people with a crack cocaine use disorder and identify salivary protein candidates for biomarkers of oral disorders. Methodology A total of 40 volunteers hospitalized for rehabilitation for crack cocaine addiction were enrolled; nine were randomly selected for proteomic analysis. Intraoral examination, report of DMFT, gingival and plaque index, xerostomia, and non-stimulated saliva collection were performed. A list of proteins identified was generated from the UniProt database and manually revised. Results The mean age (n=40) was 32 (±8.88; 18-51) years; the mean DMFT index was 16±7.70; the mean plaque and gingival index were 2.07±0.65 and 2.12±0.64, respectively; and 20 (50%) volunteers reported xerostomia. We identified 305 salivary proteins (n=9), of which 23 were classified as candidate for biomarkers associated with 14 oral disorders. The highest number of candidates for biomarkers was associated with carcinoma of head and neck (n=7) and nasopharyngeal carcinoma (n=7), followed by periodontitis (n=6). Conclusions People with a crack cocaine use disorder had an increased risk of dental caries and gingival inflammation; less than half had oral mucosal alterations, and half experienced xerostomia. As possible biomarkers for 14 oral disorders, 23 salivary proteins were identified. Oral cancer and periodontal disease were the most often associated disorders with biomarkers.
RESUMO
OBJECTIVE: To study the influence of black space between the maxillary central incisors on the aesthetic visual perception of the face, via eye-tracking and visual analogue scale (VAS). METHODS: Black space between the central incisors was created, for both sexes, as follows: control, 1-mm black space, 2-mm black space and 3-mm black space. Ninety raters participated in this study, divided into three groups: 30 laypeople, 30 nonorthodontists, and 30 orthodontists. After the visual calibration of each observer, eight photographs were presented in the Ogama® software concomitant with the use of the hardware The Eye Tribe®. Ogama generated information depending on the eye-tracking of each rater, regarding the time until the first fixation, time of fixation, heatmap, scanpath, and total time of fixation, to evaluate the areas deemed to be of interest according to the raters. Later on, the VAS was used, where each rater evaluated the images in an album on a scale of zero to 10 points. RESULTS: The eyes and mouth were the areas more often noticed by the raters according to the heatmaps, while no significant difference was observed in time until the first fixation between the three groups of raters (p> 0.05). However, regarding the time of fixation on the mouth, a significant difference was observed (p< 0.05) when comparing the three groups. CONCLUSION: Black space has a negative effect on the aesthetic perception of the face. The amount of attention on the mouth is correspondent to the size of the black space.
Assuntos
Estética Dentária , Incisivo , Tecnologia de Rastreamento Ocular , Feminino , Humanos , Incisivo/anatomia & histologia , Incisivo/diagnóstico por imagem , Masculino , Sorriso , Escala Visual AnalógicaRESUMO
ABSTRACT Objective: To study the influence of black space between the maxillary central incisors on the aesthetic visual perception of the face, via eye-tracking and visual analogue scale (VAS). Methods: Black space between the central incisors was created, for both sexes, as follows: control, 1-mm black space, 2-mm black space and 3-mm black space. Ninety raters participated in this study, divided into three groups: 30 laypeople, 30 nonorthodontists, and 30 orthodontists. After the visual calibration of each observer, eight photographs were presented in the Ogama® software concomitant with the use of the hardware The Eye Tribe®. Ogama generated information depending on the eye-tracking of each rater, regarding the time until the first fixation, time of fixation, heatmap, scanpath, and total time of fixation, to evaluate the areas deemed to be of interest according to the raters. Later on, the VAS was used, where each rater evaluated the images in an album on a scale of zero to 10 points. Results: The eyes and mouth were the areas more often noticed by the raters according to the heatmaps, while no significant difference was observed in time until the first fixation between the three groups of raters (p> 0.05). However, regarding the time of fixation on the mouth, a significant difference was observed (p< 0.05) when comparing the three groups. Conclusion: Black space has a negative effect on the aesthetic perception of the face. The amount of attention on the mouth is correspondent to the size of the black space.
RESUMO Objetivo: Avaliar a percepção visual e estética do espaço negro entre os incisivos centrais superiores, via rastreamento do olhar e escala visual analógica (EVA). Métodos: Manipulou-se o espaço negro entre os incisivos centrais superiores, para ambos os sexos, da seguinte forma: imagem controle, espaço negro de 1 mm, espaço negro de 2 mm e espaço negro de 3 mm. Participaram desse estudo noventa avaliadores, divididos em três grupos: 30 leigos, 30 cirurgiões-dentistas e 30 ortodontistas. Após a calibração visual de cada observador, foram projetadas oito fotografias no software OGAMA® em conjunto com o hardware The Eye Tribe®. O OGAMA gerou informações do rastreamento do olhar de cada avaliador com relação ao tempo até a primeira fixação, mapa de calor, trajetória do olhar e tempo total de fixação para avaliar as áreas consideradas de interesse, de acordo com os avaliadores. Posteriormente, utilizou-se a EVA para avaliar as imagens a partir de um álbum, em uma escala de 0 a 10 pontos. Resultados: Os olhos e a boca foram as áreas com maior fixação pelos avaliadores, de acordo com os mapas de calor. Os resultados estatísticos não apresentaram diferença estatisticamente significativa entre os três grupos de avaliadores (p> 0,05), quanto ao tempo até a primeira fixação. Porém, em relação ao tempo de fixação na boca, observou-se diferença estatisticamente significativa (p< 0,05) na comparação dos três grupos. Conclusão: O espaço negro apresentou um efeito negativo na percepção estética da face. O aumento de fixação na boca correspondeu ao aumento do tamanho do espaço negro.
Assuntos
Humanos , Masculino , Feminino , Negro ou Afro-Americano , Estética Dentária , Tecnologia de Rastreamento Ocular , Incisivo , Sorriso , Escala Visual Analógica , Incisivo/anatomia & histologia , Incisivo/diagnóstico por imagemRESUMO
The profiles of polytraumatized patients in intensive care units were characterized. Serum and salivary markers were compared with normality between Classes I and II of APACHE II and between periods of hospitalization; these results were correlated. This was a prospective study on saliva charts and collection (n = 70). Profile: male, 27 years old, blunt traumas and collisions. Serum parameters with normality: decrease in pH, creatinine at admission to Class I, and at 48 and 72 hours in both classes; K+ at 48 h in Class II; Ca+ on admission in both classes and at 72 h in Class I. Increase in urea at 72 h in Class II, glucose at all times and in all classes, and Ca+ at 48 h in both classes. Class II had high Na+ at 48 and 72 h compared to Class I. In Class I, creatinine reduction occurred in 48 h and 72 h compared to admission and an increase of Ca+ at 48 h with admission. In Class II, pH and Na+ increased at 48 h and 72 h compared to admission. K+ decreased from admission to 48 h and increased from 48 h to 72 h. Urea increased from 48 to 72 hours. Creatinine decreased from admission to 48 and 72 hours. Ca+ increased from admission to 48 hours and decreased from 48 to 72 hours. There was an increase in the saliva levels in both classes and times in relation to normality. There was an increase in urea at admission, glucose at 72 h, and Ca+ at 48 h in Class II compared with Class I. Class I urea increased from admission to 48 h and Ca+ decreased from admission to 48 h. Class II urea decreased from 48 h to 72 h. Strong or very strong positive correlation was identified between blood and creatinine saliva at all times and regular and negative Ca+ at 72 h. This study provides evidence that salivary and serum biomarkers can be used together to monitor the evolution of the clinical symptoms of ICU patients.
Assuntos
Biomarcadores/sangue , Traumatismo Múltiplo/sangue , Ácido N-Acetilneuramínico/sangue , APACHE , Adolescente , Adulto , Idoso , Feminino , Hospitais Universitários , Humanos , Unidades de Terapia Intensiva , Masculino , Pessoa de Meia-Idade , Saliva/metabolismo , Adulto JovemRESUMO
Ao longo da história, o ensino e o aprendizado nos Cursos de Odontologia no Brasil vem sendo exercidos com ênfase no desenvolvimento de conhecimentos científicos e habilidades técnicas que são indispensáveis para a profissão. Porém, considerando-se as atuais necessidades apresentadas pela sociedade moderna, a importância em propiciar ao estudante de graduação em Odontologia uma formação integral, holística e baseada em competências, que vão além do tecnicismo, torna-se emergente. O presente artigo tem como objetivo relatar a experiência da utilização de metodologias ativas, com atividades lúdicas, como estratégia pedagógica para a promoção do ensino-aprendizagem, baseada em competências, na disciplina de Estomatologia, do Curso de Odontologia, da Pontifícia Universidade Católica do Paraná. De acordo com os relatos apresentados pelos estudantes desta disciplina, conclui-se que as metodologias ativas podem ser utilizadas como estratégia pedagógica complementar para o ensino e aprendizagem em Odontologia (AU).
Traditionally, teaching and learning of Dentistry courses in Brazil have focused on the development of scientific knowledge and technical skills inherent to the profession. However, when considering the demands of today's society, the importance of providing undergraduate Dentistry students with integral, holistic, and competence-based training that goes beyond the profession's technical requirements becomes clear. This article reports the experience of applying active methodologies, including playful activities, as a pedagogic strategy for the promotion of teaching and learning, based on competence-based training, in the Stomatology course at the Dental School of the Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil. According to undergraduate students' reports, it was concluded that such active methodologies could be used as a complementary pedagogic strategy for teaching and learning in Dentistry (AU).
Assuntos
Humanos , Masculino , Feminino , Estudantes de Odontologia , Educação Baseada em Competências , Aprendizagem Baseada em Problemas/métodos , Educação em OdontologiaRESUMO
OBJECTIVE: To report a case of a challenging oral diagnosis involving a primary diffuse large B cell lymphoma of the oral cavity mimicking a hyperplastic reactive lesion. CASE REPORT: A 72-year-old male patient was referred to the Department of Stomatology presenting a proliferative nodular lesion in the anterior region of the mandible involving the anterior teeth. The clinical examination revealed anterior teeth affected by periodontal disease, suggesting the nodular cession hyperplastic reaction. Incisional biopsy was performed under local anesthesia. The histopathological examination revealed a diffuse proliferation of atypical large lymphoid cells. The tumor cells showed immunopositivity for CD20 and Ki67 (100%) and negativity for CD3, CD30, and CD15. The diagnosis of diffuse large B cell lymphoma was established. The patient underwent chemotherapy and progressed to death after nine months. CONCLUSION: Lymphomas of the oral cavity are rare and may have nonspecific clinical features, mimicking inflammatory and reactive lesions. Therefore, a detailed clinical evaluation associated with histopathological and immunohistochemical analysis should be performed to enable early and accurate diagnoses in suspected oral lesions.
RESUMO
BACKGROUND: The oral mucosa is an important defense barrier to penetration of microorganisms. Thus, changes in the oral epithelium might indicate risk for infection in intensive care patients receiving mechanical ventilation. OBJECTIVE: To evaluate the oral mucosa of intensive care patients who did or did not receive mechanical ventilation by using liquid-base exfoliative cytology. METHODS: The sample consisted of 3 groups: 27 patients admitted to intensive care during a 7- to 14-day period who received mechanical ventilation, 29 patients admitted during the same period who did not receive mechanical ventilation, and 27 healthy patients who had no lesions in the mouth. For all 3 groups, samples were collected from the buccal mucosa by using cytology brushes. Smears were applied to glass slides before Papanicolaou staining and were codified for blind analyses by a cytopathologist. Kruskal-Wallis and Dunn tests were used to analyze the results. RESULTS: Patients receiving mechanical ventilation had higher prevalence and intensity of karyomegaly, perinuclear halos, cell keratinization, deep cells, and leukocyte infiltrates than did patients in the other 2 groups (P < .05). No significant differences were observed between the control group and the group who did not receive mechanical ventilation. CONCLUSIONS: Liquid-base exfoliative cytology can be used to detect preclinical alterations in the oral mucosa. Patients treated with mechanical ventilation are vulnerable to infections, and oral care may be valuable in their prognosis.
Assuntos
Mucosa Bucal/citologia , Mucosa Bucal/patologia , Respiração Artificial/efeitos adversos , Idoso , Núcleo Celular/patologia , Cuidados Críticos , Feminino , Humanos , Leucócitos/patologia , Biópsia Líquida , Masculino , Pessoa de Meia-Idade , Teste de PapanicolaouRESUMO
OBJECTIVE: Investigate the association of clinical, cytological and genetic characteristics with benign migratory glossitis (BMG). STUDY DESIGN: Sample consisted of 175 patients, 44 with BMG and 131 control patients. Clinical examination and DMFT index were assessed. Cytological evaluation determined cell morphology and morphometry. Genetic evaluation was performed by analysing IL6 polymorphisms by real-time PCR. Univariate and multivariate analyses were performed (p<0.05). RESULTS: There was a higher level of anxiety, DMFT score and a prevalence of fissured tongue in BMG group. A high mean nuclear/cytoplasmic area ratio was observed in patients with BMG. There was predominance of Papanicolaou class II I BMG group. IL6 allele G rs2069843 polymorphism was associated with BMG in the dominant model. In multivariate analysis, DMFT and anxiety scale remained associated with BMG.
Assuntos
Glossite Migratória Benigna/genética , Glossite Migratória Benigna/patologia , Adulto , Alelos , Ansiedade/genética , Ansiedade/patologia , Brasil/epidemiologia , Feminino , Predisposição Genética para Doença , Glossite Migratória Benigna/epidemiologia , Glossite Migratória Benigna/psicologia , Humanos , Interleucina-6/genética , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Prevalência , Fatores Socioeconômicos , Língua Fissurada/epidemiologia , Língua Fissurada/genéticaRESUMO
Introduction: Few studies succeeded on demonstrating that dentitions are unique. Methodological limitations may have influenced these outcomes. Objective: The present study aims to validate software packages for comparing human dentitions. Material and methods: A pool of 40 dental casts were laser scanned (XCAD 3D®, XCADCAM Technology®, São Paulo, Brazil) and implemented in Geomagic Studio® (GS) (3D Systems®, Rock Hill, USA), Cloud Compare® (CC) (Telecom Paris Tech® and EDF®, Paris, France), and Maestro 3D Ortho Studio® (MS) (AGE Solutions®, Pontedera, Italy) software packages to evaluate metric and superimposition tools. Results: Software performances did not significantly differ (p>0.05) considering cropping, landmarking and superimposition functions. GS was more precise for detecting identical models (p>0.05. Inter and intra examiner reproducibility reached optimal outcomes. Calibration was assured for software measuring tools and scanning process. Conclusion: Both GS and CC may be used for comparing 3D anterior dentitions. However, more practical and less operator-depending procedures are available in GS.
Introdução: Poucos estudos obtiveram êxito em demonstrar a unicidade da dentição humana. Falhas metodológicas podem ter influenciado os resultados destes estudos. Objetivo: O presente estudo objetivou validar ferramentas contidas em pacotes de software existentes para comparar dentições humanas. Material e método: Uma amostra de 40 modelos odontológicos digitalizados (XCAD 3D®, XCADCAM Technology®, São Paulo, Brazil) foi selecionada e analisada utilizando os seguintes softwares: Geomagic Studio® (GS) (3D Systems®, Rock Hill, USA), Cloud Compare® (CC) (Telecom Paris Tech® and EDF®, Paris, France), and Maestro 3D Ortho Studio® (MS) (AGE Solutions®, Pontedera, Italy). Resultados: Os softwares não apresentaram performances com diferença estatisticamente significante (p>0.05) considerando os procedimentos de recorte, colocação de pontos de referência (landmarks) e sobreposição de modelos. O software GS apresentou maior precisão para detectar modelos idênticos ((p>0.05). Testes intra e interexaminador resultaram em ótima concordância. Os softwares apresentaram ótima calibração de ferramentas métricas. Conclusão: Ambos os softwares GS e CC podem ser utilizados para comparar modelos odontológicos digitalizados. Contudo, performances mais práticas e independentes do operador podem ser alcançadas por meio do software GS.
Assuntos
Odontologia Legal , Ciências Forenses , National Institute of Biomedical Imaging and Bioengineering (U.S.) , DenteRESUMO
The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.
O propósito deste estudo foi avaliar o potencial proliferativo celular das lesões de líquen plano bucal (LPB) de pacientes sem vírus da hepatite C (VHC) por meio do método AgNOR, comparando-o ao potencial proliferativo celular da mucosa bucal normal de portadores de VHC, tratados ou não com interferon e ribavirina. Um estudo transversal foi realizado para investigar 4 grupos: 10 pacientes VHC+ sem sinais clínicos de LPB que nunca haviam sido tratados para a infecção por VHC - Grupo 1; 10 pacientes VHC+ que estavam sob tratamento com interferon e ribavirina - Grupo 2; 15 pacientes com LPB reticular histopatologicamente confirmado, sem VHC - Grupo 3; e 15 doadores de sangue sem infecção por VHC e sem sinais clínicos de LPB (Grupo 4 - Grupo de Controle). O material celular de todos os grupos foi coletado pela técnica da citologia em base líquida. Então, o material sedimentado de cada paciente foi submetido ao método da impregnação das regiões organizadoras nucleolares pela prata (AgNOR). A contagem das NORs foi realizada em 100 núcleos celulares epiteliais por paciente por meio do programa Image Tool(r). O teste Tukey HSD foi utilizado para comparar o valor médio de NORs entre os grupos e mostrou que a mucosa bucal dos pacientes VHC+ previamente tratados com fármacos anti-VHC (Grupo 2) apresentou maior número médio de NORs por núcleo em relação aos outros (p<0,05). O tratamento anti-VHC pode estar relacionado ao aumento da atividade proliferativa celular da mucosa bucal, aventando uma possível relação entre LPB e pacientes VHC+ tratados com interferon e ribavirina.
Assuntos
Animais , Bovinos , Humanos , Ratos , Genes , RNA Polimerase II/metabolismo , Fatores Genéricos de Transcrição , Transcrição Gênica , Fatores de Elongação da Transcrição , Fatores de Transcrição/metabolismo , Núcleo Celular/metabolismo , Detergentes/farmacologia , Genes/efeitos dos fármacos , Células HeLa/metabolismo , Heparina/farmacologia , Histonas/genética , Fígado/metabolismo , Plasmídeos , Regiões Promotoras Genéticas , Sarcosina/análogos & derivados , Sarcosina/farmacologia , Moldes Genéticos , Timo/enzimologia , Fatores de Transcrição/isolamento & purificação , Transcrição Gênica/efeitos dos fármacosRESUMO
The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.
Assuntos
Hepatite C/complicações , Líquen Plano Bucal/patologia , Antivirais/uso terapêutico , Brasil , Proliferação de Células , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Feminino , Hepatite C/tratamento farmacológico , Humanos , Interferons/uso terapêutico , Masculino , Mucosa Bucal/patologia , Ribavirina/uso terapêutico , Coloração pela PrataRESUMO
Several studies described tooth development as a reliable pathway for age estimations. Depending on the considered life span, the dental age indicators vary. In children, combinations of developing teeth provide the best information about age. In sub adults third molar mineralization is almost exclusively considered. The aim of this study was, firstly, to verify the Willems model in a Brazilian sample. Secondly, to observe differences between the Willems model and a new developed Brazilian model. Thirdly, the information of permanent teeth (PM) and third molar (TM), development was combined for age estimation in children. A sample of 1357 panoramic radiographs of Brazilian males (M) and females (F), with age between 5 and 23 years was collected. The technique of Gleiser and Hunt modified by Kohler (1955) [34] was applied for third molar staging in the entire sample. The Demirjian staging technique was used on the mandibular left permanent teeth (except third molars) of all individuals from 5 to 15 years. Kappa and weighted Kappa statistics were performed to verify inter- and intra-observer reliabilities. Based on the obtained Demirjian scores the Willems model was verified. Next the data were split to develop a new Brazilian model based on the Willems method and to verify the established model. The accuracy in age prediction between the Willems model and the new Brazilian model was compared. Additionally, regression models including PM, TM and PM plus TM information were compared. The Kappa and weighted Kappa statistics revealed high agreement between observers (0.88 Kappa; 0.93 weighted Kappa). The differences between predicted and chronological age for the verified Willems model were expressed in mean errors of -0.17 and -0.38 year for F and M respectively. The differences in mean error between the new developed Brazilian model and the Willems model were 0.02 (F) and 0.20 (M) year. The regression models combining PT and TM information provided only in the age range between 14 and 15.99 years a small decrease in root mean squared error (0.2 year) in females. The new developed Brazilian model provided similar age predicting performances as the Willems et al. model. Added TM was only providing more accurate age estimations in the ages of 14 and 15 year in F.
Assuntos
Determinação da Idade pelos Dentes/métodos , Dentição Permanente , Dente Serotino/crescimento & desenvolvimento , Adolescente , Adulto , Brasil , Criança , Pré-Escolar , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Masculino , Dente Serotino/diagnóstico por imagem , Radiografia Dentária Digital , Radiografia Panorâmica , Análise de Regressão , Adulto JovemRESUMO
Dentin dysplasia is a genetic disorder of the teeth that affects the dentin and the pulp. Type I is sometimes called "rootless teeth," because of the loss of organization of the root dentin, which often leads to a shortened root length. The purpose of this article was to present a rare clinical case of a girl who was diagnosed with dentin dysplasia type I when she was referred for an orthodontic evaluation. Panoramic and periapical radiographs showed defective root formation and areas with periapical radiolucencies in several teeth. Her Angle Class I malocclusion was successfully treated, providing esthetic and functional results, without clinical symptoms or signs of periodontitis or odontogenic infections.
Assuntos
Displasia da Dentina/complicações , Má Oclusão Classe I de Angle/terapia , Ortodontia Corretiva/métodos , Raiz Dentária/anormalidades , Criança , Displasia da Dentina/patologia , Feminino , Humanos , Má Oclusão Classe I de Angle/complicações , Ortodontia Corretiva/instrumentação , Resultado do TratamentoRESUMO
Nowadays, technological advances are becoming more and more important in forensic sciences. Yet autopsy is still one of the very traditional methods. This also applies for dentalautopsies, in which visual, photographic and radiological evidences are collected. In this context, Virtual Autopsy appears as a helpful and complementary tool for dental and medical cadaveric examination. Usinghigh-tech radiological approaches, Virtual Autopsy may provide, through images, an efficient and more accurate view on the individual case. This critical review aims to update the dental professionalspresenting the first national paper with explanations on Virtual Autopsy.
Atualmente os avanços tecnológicos se fazem cada vez mais importantes nas ciências forenses. Por outro lado a autópsia ainda é uma abordagem tradicional na prática médica-odontológica, na qual evidências são coletadas por meio fotográfico e radiológico. Neste contexto, a Autópsia Virtual surge como uma ferramenta útil e complementar para o exame cadavérico. Através da alta tecnologia radiológica a Autópsia Virtual fornece uma visão eficiente e precisa do caso a ser analisado. Esta revisão crítica tem o objetivo de atualizar os Cirurgiões-dentistas apresentando o primeiro trabalho com o conteúdo voltado a Autópsia Virtual.
Assuntos
Autopsia , Odontologia Legal , Radiologia , Medicina LegalRESUMO
As doenças nas quais o sistema imunológico causa lesões no próprio organismo chamam-se doenças autoimunes, a exemplo do pênfigo, que é considerado um conjunto de doenças vesicobolhosas que podem acometer membranas mucosas e pele.Objetivo: apresentar uma revisão de literatura atualizada acerca do pênfigo, englobando sua conceituação e etiologia, características clínicas, formas de diagnóstico, tratamento e prognóstico.Conclusão: o pênfigo é uma enfermidade crônica autoimune que com frequência se manifesta como lesões bolhosas iniciais na mucosa bucal, afetando posteriormente outras mucosas e a pele. Além disso, apresenta uma diversidade de lesões que podem ser incluídas no seu diagnóstico diferencial, as quais devem ser detalhadamente conhecidas pelo cirurgião dentista...
Diseases in which the immune system causes lesions in the body are called autoimmune diseases, such as pemphigus, which is considered a set of vesiculobullous diseases that can affect the mucous membranes and skin.Aim: to present a review of current literature on pemphigus, including its concept and etiology, clinical features, methods of diagnosis, treatment and prognosis.Conclusion: the pemphigus is a chronic autoimmune disease that often manifests itself as early bullous lesions in the oral mucosa, subsequently affecting other mucous membranes and skin. Moreover, it presents a variety of lesions that may be included in their differential diagnosis, which must be thoroughly known by the surgeon-dentist...