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El Schwannoma se origina de la vaina perineural de Schwannoma, se detecta con frecuencia incidentalmente en estudios imagenológicos siendo el principal método diagnóstico la Tomografía Computada. El tratamiento es la resección quirúrgica con márgenes libres. Se presenta una paciente femenina de 49 años, en control por oncología por enfermedad de base, cáncer de mama izquierda, se identifica por TAC y PECT/TC imagen voluminosa en retroperitoneo situación lateroaórtica izquierda de configuración no quística e hipermetabólica, solicita biopsia percutánea, ante la falta de ventana, se decide exeresis completa de masa. Diagnóstico definitivo patológico Schwannoma. Sin indicación de tratamiento adyuvante, cursa buena evolución postoperatoria sin recidiva.

Schwannoma, a benign tumor that arises from Schwann cells of the perineural nerve sheath, is often incidentally detected in imaging tests and mainly diagnosed by CT scan. Treatment consists of surgical resection with clear margins. We present the case of a 49-year-old female patient subject to Oncology Department follow-up due to an underlying disease, left breast cancer. A large, hypermetabolic, noncystic mass in the retroperitoneal region is identified by CT and PECT/CT scan in the left lateral aortic area. A percutaneous biopsy is requested. Due to the limited acoustic window, complete resection of the mass is decided. Final histopathology diagnosis of Schwannoma. No adjuvant treatment indication; undergoing favorable postoperative progress, without recurrence

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BACKGROUND: Bacillus cereus is associated with milk, dairy product, and dairy farm contamination. The aim of this study was to characterize strains of B. cereus in the small-scale artisanal cheese production chain in southwestern Mexico. METHODS: 130 samples were collected. B. cereus isolation was performed on Mannitol Egg Yolk Polymyxin (MYP) agar. Genotyping, enterotoxigenic profile, and determination of genes involved in the formation of B. cereus biofilm were performed by PCR. An antimicrobial susceptibility test was made by broth microdilution assay. The phylogenetic analysis was performed by amplification and sequencing of 16s rRNA. RESULTS: B. cereus sensu lato was isolated and molecularly identified in 16 samples and B. cereus sensu stricto (B. cereus) was the most frequently isolated and identified species (81.25%). Of all the isolated B. cereus sensu lato strains, 93.75% presented at least one gene for some diarrheagenic toxins, 87.5% formed biofilms, and 18.75% were amylolytic. All B. cereus sensu lato strains were resistant to beta-lactams and folate inhibitors. A close phylogenetic relationship between isolates was found between the cheese isolates and the air isolates. CONCLUSIONS: Strains of B. cereus sensu lato were found in small-scale artisanal cheeses on a farm in southwestern Mexico.

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DNA methylation is a key epigenetic modification to regulate gene expression in mammalian cells. Abnormal DNA methylation in gene promoters is common across human cancer types. DNMT3B is the main de novo methyltransferase enhanced in several primary tumors. How de novo methylation is established in genes related to cancer is poorly understood. CpG islands (CGIs), common sequences, and transcription factors (TFs) that interact with DNMT3B have been associated with abnormal de novo methylation. We initially identified cis elements associated with DNA methylation to investigate the contribution of DNMT3B overexpression to the deregulation of its possible target genes in an epithelial cell model. In a set of downregulated genes (n = 146) from HaCaT cells with DNMT3B overexpression, we found CGI, common sequences, and TFs Binding Sites that interact with DNMT3B (we called them P-down-3B). PPL1, VAV3, IRF1, and BRAF are P-down-3B genes that are downregulated and increased their methylation in DNMT3B presence. Together these findings suggest that methylated promoters aberrantly have some cis elements that could conduce de novo methylation by DNMT3B.

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SARS-CoV-2 contains four structural proteins, two of which, the spike and nucleocapsid, are commonly used for the standardization of novel methods for antibody detection; however, some limitations in their use have been observed due to the homology of this virus with other phylogenetically-related viruses. We performed in silico analysis to search for novel immunogenic and antigenic peptides. A total of twenty-five peptides were preliminarily selected, located in the 3D structure of both proteins. Finally, eight peptides were selected: one located in the N protein and seven in the S1 domain of the spike protein. Additionally, the localization of selected peptides in 2D structures and possible changes in the sequences of these peptides in SARS-CoV-2 variants of concern were analyzed. All peptides were synthetized in MAP8 format, and recombinant S (trimer and RBD) and N proteins were used as antigens to search for antibodies in serum samples derived from COVID-19 patients, and for antibody response in New Zealand rabbits. Results showed high recognition of the serum derived from COVID-19 patients to all selected peptides; however, only the RBD3 peptide induced antibody production. In conclusion, this work provides evidence for a new strategy in peptide selection and its use for antibody detection or antibody production in animals.

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Helicobacter pylori promotes the secretion of cytokines that regulate inflammation and carcinogenesis. Immune cells secrete cytokines into the extracellular medium or packaged in exosomes. The objective of this study was to analyze the profile of soluble and exosomal cytokines that were secreted by human peripheral blood mononuclear cells (PBMCs) that were infected with H. pylori and to build a network of interaction between cytokines and cellular proteins. PBMCs were obtained by density gradient centrifugation and infected with H. pylori for 24 h. The infection was verified by immunofluorescence and Western blot for CagA. The exosomes were obtained from culture supernatant by ultracentrifugation and characterized by transmission electron microscopy, particle size analysis, and Western blot for CD9 and CD81. Cytokines were quantified using a multiplex immunoassay in the culture supernatant, intact exosomes, and lysed exosomes. H. pylori adheres to lymphocytes and translocates CagA. In PBMCs, H. pylori induces an increase in the soluble and exosomal IL-1ß, IL-6, TNF-α, IL-10, IL-17A, IL-21, and IL-22. The protein-protein interaction (PPI) network shows that soluble and exosomal cytokines interact with proteins that participate in signaling pathways such as NF-κB, MAPK, PI3K-Akt, Jak-STAT, FoxO, and mTOR, that are related to carcinogenesis; moreover, TNF-α had the highest number of interactions. Cytokine-loaded exosomes represent another means of intercellular communication that is activated by H. pylori to stimulate inflammation, carcinogenesis, or cancer progression. Cytokine-loaded exosomes are likely to be associated with extragastrointestinal diseases of inflammatory origin.

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Literature reports indicate that during bracket removal there can be enamel damage. We compare the shear bond strength (SBS) and tooth enamel loss of four adhesive systems and identify the Ca/P ratio. Then a total of 20 premolars were divided into four groups of five each. After prophylaxis, photographs were taken at 35× with a scanning electron microscope (SEM) and analyzed with X-ray spectroscopy (EDS) at 250×. Brackets were bonded with Transbond™ MIP(G1), Transbond™ PLUS SEP(G2), Enlight(G3) and Stylus®(G4) adhesives, 24 h after were debonded with a Instron universal testing machine at 1 mm/min. All the brackets were photographed with the SEM. The amount of lost enamel was measured with AutoCad. All the results were measured with a significance level p < .05. The SBS general average at debonding was 7.94 ± 2.26 MPa, meanwhile the SBS for G1, G2, G3 and G4 was 9.38 ± 1.46, 6.28 ± 0.69, 9.08 ± 2.45 and 7.04 ± 2.64 MPa respectively. 90% of the samples had no enamel loss, 10% had enamel loss. Only two samples in G1 presented an enamel loss area of 0.34mm2 and 0.80mm2 respectively. From EDS analysis, the Ca/P ratio was 1.6 ± 0.05, 1.61 ± 0.03, 1.64 ± 0.83 and 1.59 ± 0.07 for G1, G2, G3 and G4 respectively; no statistically significant differences were found. We conclude that no association was found between the Ca/P ratio and enamel damage when brackets are removed. HIGHLIGHTS: Where enamel is lost, we observe fractures, steps, horizontal and vertical enamel loss. There is a loss of tooth enamel from 0.34 to 0.80 mm2 with Transbond PLUS SEP. Structural loss of enamel is almost inevitable during the separation of the bracket.

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Insulin is the hormone responsible for maintaining glucose homeostasis in the body, in addition to participating in lipid metabolism, protein synthesis, and the inhibition of gluconeogenesis. These functions are well characterized in the classic organ target cells that are responsible for general energy regulation: the liver, skeletal muscle, and adipose tissue. However, these actions are not restricted to these tissues because insulin has been shown to affect most cells in the body. This review describes the role of insulin in leukocyte signaling pathways, metabolism and functions, and how insulin resistance could affect this signaling and deteriorate leukocyte metabolism and function, in addition to showing evidence that suggests leukocytes may substantially contribute to the development of systemic insulin resistance.

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Objetivos: Presentar una resección quirúrgica R0, con fines curativos de un cáncer de vesícula biliar estadio 1B. Caso Clínico: Femenina de 60 años de edad, sin antecedentes de relevancia, que presenta por estudios complementarios alta sospecha de cáncer de vesícula biliar (T2N0Mx), se realiza laparotomía exploradora con colecistectomía convencional, bisegmentectomía 4 b y 5 del hígado y vaciamiento ganglionar. Sin complicaciones con alta sanatorial al 4 día post operatorio. Conclusión: El diagnostico precoz y una cirugía temprana en el cáncer de vesicula biliar puede ser una herramienta fundamental para poder ofrecer al paciente una cirugía curativa en este tipo de patología

Objects: This paper introduces an R0 surgical resection in the treatment of stage 1B gallbladder cancer. Discussion: 60-year female patient without medical history with highly suspect gallbladder cancer (T2N0Mx), revealed by clinical exams. The patient underwent an exploratory laparotomy, a conventional cholecystectomy, a double segmentectomy for 4b-5 liver, and the lymph node was removed. Complications were not reported, and the patient was discharged four days after surgery. Conclusion: Early diagnosis and early surgery on gallbladder cancer patients may prove essential to cure such pathology

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The clinically adequate shear bond strengths (SBS) should be from 2.8 to 10 MPa. The aim of this research is to observe tooth enamel loss through a scanning electron microscope (SEM) during the debonding of braces of four adhesive systems. Then, 100 premolars were used in 4 groups of 25 specimens each, for Transbond MIP (G1), Enlight (G2), Stylus (G3), and Transbond Plus SEP (G4). The research was done under the NOM ISO/TS 11405:2015. Gemini 3M were placed under the manufacturer's recommendations. The SBS test was done at 24 hr in an Instron electromechanical universal testing machine at 1 mm/1 min. Adhesive remnant index (ARI) was measured, all of the brackets where examined in the SEM. For the shear bonding strength G1 = 10.09 ± 2.73 MPa, G2 = 9.27 ± 3.99 MPa, G3 = 7.83 ± 4.46 MPa, and G4 = 6.40 ± 2.85 MPa statistically significant differences were found when comparing the four groups (p = .002). In the Tukey post hoc test, G1 versus G4 and G2 versus G4, statistically significant differences were found. For the ARI a value of 1 in 46%, followed by a value of 2 in 38%, a value of 3 in 13% and a value of 0 in 3% of the total samples, finding statistically significant differences (p < .001). In relation to the tooth enamel loss due to SBS, statistically significant differences were found (p = .326). G1 and G4 had not statistically significant differences. Even though our results concur with the appropriate clinical values, we observed tooth enamel loss with Transbond Plus SEP and Stylus.

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Resumen Se reflexiona en torno al diseño normativo que regula la investigación en Colombia y las prácticas de los investigadores desde un enfoque ético, abordando asuntos pro blemáticos en las ciencias de la salud, las ciencias sociales y las disciplinas ambien tales, para concluir que se necesita fortalecer el control del Estado en estos temas, la actualización de la normatividad y una mayor formación de los investigadores y miembros de Comités de Ética en investigación, especialmente en: la protección de los participantes, el cuidado de los animales, los conflictos de interés, el manejo de comunidades y de grupos vulnerables, los protocolos comunitarios, el consentimien to informado, la consulta previa, el cuidado de la biodiversidad y el acceso a recursos genéticos.

Abstract This paper reflects on the normative design, which regulates research in Colombia and the practices of researchers from an ethical approach, by addressing critical issues in the health sciences, social sciences, and environmental disciplines, to conclude that it is necessary to strengthen state control in these issues, the updating of the regulations and greater training of researchers and members of Ethics Committees in research , especially in the protection of participants, animal care, conflicts of interest, the management of communities, and vulnerable groups, community pro tocols, informed consent, prior consultation, biodiversity care, and access to genetic resources.

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In Brazil's food industry, dairy production is one of the most important sectors, whose most relevant byproduct is whey. Due to the difficulties of reuse and environmental impacts caused when discarded as effluent in water bodies, an alternative for its final destination would be the application of this residue in the soil. The purpose of this study was to determine chemical changes and mobility and distribution of solutes in the soil after applications of whey rates, as well as to analyze the leachate collected after each application. The test was carried out in a laboratory, in PVC columns filled with soil. The treatments consisted of 2 x 2 samples of a typical dystrophic Red-Yellow Oxisol (Oxisol) and a typical dystrophic fluvic Inceptisol (Inceptisol), sampled in the layers 0- 20 and 20-40 cm. Each experimental unit consisted of 11 PVC rings (diameter of 6.6 cm, height of 7 cm). The columns were arranged in a randomized complete block design with five replications. Four whey rates were applied, corresponding to a soil pore volume of 0.2, at intervals of six days. The leachate was collected 24 and 120 hours after each application to measure pH, electrical conductivity (EC), chemical oxygen demand (COD), contents of total N, N-NH4+, N-NO3-, Na, K, Ca, and Mg. Six days after the last whey application, the columns were opened and the soil of each ring was analyzed for pH, EC, total N, N-NH4+, N-NO3-, Na, K, Ca, and Mg. The high electrolyte concentrations of whey resulted in a general increase in soil EC. The increase of N-NH4+ and N-NO3- in the soil was high due to mineralization. High concentrations of K, Na and Ca caused displacement of Mg from the exchange complex. It was concluded that from an environmental standpoint, whey soil application is a viable alternative, given that problems of salinization and leaching of undesirable elements are avoided by an adequate management.

Entre os setores da indústria alimentícia, o segmento de laticínios é um dos mais importantes do Brasil, sendo o soro de leite o seu maior subproduto. Devido às dificuldades de reaproveitamento e aos impactos ambientais causados, quando descartado como efluente em corpos de água, uma alternativa para a sua destinação final seria a aplicação desse resíduo no solo. Objetivou-se com este trabalho determinar alterações químicas e a mobilidade e distribuição de solutos no solo após aplicação fracionada de soro de leite, assim como a caracterização dos percolados recolhidos após cada aplicação. O ensaio, em laboratório, foi conduzido em colunas de PVC preenchidas com solo. Os tratamentos corresponderam a um fatorial 2 x 2, sendo amostras de um Latossolo Vermelho-Amarelo distrófico típico (LVAd) e de um Cambissolo Flúvico Tb distrófico (CYbd), coletados em duas profundidades: 0­20 e 20­40 cm. Cada unidade experimental foi constituída de 11 anéis de PVC, com 6,6 cm de diâmetro interno e 7 cm de altura. As colunas foram dispostas em um delineamento experimental em blocos casualizados, com cinco repetições. Foram realizadas quatro aplicações de soro de leite, correspondentes a 0,2 volume de poros de solo, a cada intervalo de seis dias. O percolado foi recolhido após 24 e 120 horas de cada aplicação sendo realizadas as seguintes determinações: pH, condutividade elétrica (CE), demanda química de oxigênio (DQO), teores de N total, N-NH4+, N-NO3-, Na, K, Ca e Mg. Após seis dias da última aplicação de soro de leite, as colunas foram desmontadas e o solo de cada anel analisado. As análises compreenderam: pH em água, CE (1:5), N total, N-NH4+, N-NO3-, Na, K, Ca e Mg. As elevadas concentrações eletrolíticas do soro de leite provocaram aumento generalizado da CE no solo. Houve incremento maior do nitrogênio assimilável (N-NH4+ e N-NO3-) no solo em decorrência de processos de mineralização. Elevadas concentrações de K, Na e Ca causaram deslocamento de Mg do complexo de troca. Conclui-se que a aplicação de soro de leite no solo é uma alternativa viável do ponto de vista ambiental, desde que seja feito um correto manejo, a fim de evitar problemas de salinização e perdas por lixiviação de elementos indesejáveis.

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BACKGROUND: HPV16 infection is one of the main risk factors involved in the development of cervical cancer, mainly due to the high oncogenic potential of the viral proteins E6 and E7, which are involved in the different processes of malignant transformation. There is a broad spectrum of intratypical variation of E6, which is reflected in its high diversity, biological behavior, global distribution and risk of causing cervical cancer. Experimental studies have shown that the intratypical variants of the protein E6 from the European variants (E-G350, E-A176/G350, E-C188/G350) and Asian-American variants (AAa and AAc), are capable of inducing the differential expression of genes involved in the development of cervical cancer. RESULTS: An in silico analysis was performed to characterize the molecular effects of these variations using the structure of the HPV16 E6 oncoprotein (PDB: 4XR8; chain H) as a template. In particular, we evaluated the 3D structures of the intratypical variants by structural alignment, ERRAT, Ramachandran plots and prediction of protein disorder, which was further validated by molecular dynamics simulations. Our results, in general, showed no significant changes in the protein 3D structure. However, we observed subtle changes in protein physicochemical features and structural disorder in the N- and C-termini. CONCLUSIONS: Our results showed that mutations in the viral oncogene E6 of six high-risk HPV16 variants are effectively neutral and do not cause significant structural changes except slight variations of structural disorder. As structural disorder is involved in rewiring protein-protein interactions, these results suggest a differential pattern of interaction of E6 with the target protein P53 and possibly different patterns of tumor aggressiveness associated with certain types of variants of the E6 oncoprotein.

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Epithelial-mesenchymal transition (EMT) is a reversible cellular process, characterized by changes in gene expression and activation of proteins, favoring the trans-differentiation of the epithelial phenotype to a mesenchymal phenotype. This process increases cell migration and invasion of tumor cells, progression of the cell cycle, and resistance to apoptosis and chemotherapy, all of which support tumor progression. One of the signaling pathways involved in tumor progression is the MAPK pathway. Within this family, the ERK subfamily of proteins is known for its contributions to EMT. The ERK subfamily is divided into typical (ERK 1/2/5), and atypical (ERK 3/4/7/8) members. These kinases are overexpressed and hyperactive in various types of cancer. They regulate diverse cellular processes such as proliferation, migration, metastasis, resistance to chemotherapy, and EMT. In this context, in vitro and in vivo assays, as well as studies in human patients, have shown that ERK favors the expression, function, and subcellular relocalization of various proteins that regulate EMT, thus promoting tumor progression. In this review, we discuss the mechanistic roles of the ERK subfamily members in EMT and tumor progression in diverse biological systems.

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High-risk human papillomavirus (HPV) is the primary cause of cervical carcinoma (CC). Viral integration into the host chromosomes is associated with neoplastic progression, and epigenetic changes may occur as a result. The objective of the present study was to analyze HPV L1 gene methylation and to compare the use of quantitative polymerase chain reaction (qPCR), in situ hybridization (ISH) and L1 methylation analysis as methods for detecting HPV integration. Cervical scrapes or biopsy samples positive for HPV 16 or 18, from 187 female patients with CC, squamous intraepithelial lesions (SILs) or no intraepithelial lesion (non-IL) were analyzed. Methylation of the L1 gene was determined using bisulfite modification followed by PCR, and HPV integration was subsequently analyzed. HPV 16 L1 gene methylation was revealed to increase with histological grade, with statistically significant differences observed as follows: Low-grade SIL vs. CC, P<0.0001 and non-IL vs. CC, P<0.0001. HPV 18 L1 gene methylation also increased according to histological grade, however, no statistically significant differences were observed. Methylation at CpG site 5608 of the HPV 16 L1 gene was associated with all grades of cervical lesions, whereas methylation at CpG site 5617 demonstrated the strongest association with CC (odds ratio, 42.5; 95% confidence interval, 4.7-1861; P<0.0001). The concordance rates between the various methods for the detection of the physical status of HPV 16 and HPV 18 were 96.1% for qPCR and ISH, 76.7% for qPCR and L1 gene methylation, and 84.8% for ISH and L1 gene methylation. In conclusion, methylation of the HPV 16 L1 gene increases significantly according to the grade of the cervical lesion, and methylation at CpG sites 5608 and 5617 of this gene may be used as prognostic biomarkers. ISH and L1 gene methylation have good concordance with qPCR with regards to the detection of HPV integration. Therefore, these are useful methods in determining the physical state of HPV.

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90% of cases of intestinal obstruction caused by intestinal malrotation occur in children under 1 year of age, being very uncommon in adults. A female patient of 27 years of age consults with abdominal pain, vomiting, abdominal distention, dehydration and signs of severe malnutrition. After EGDS, CT scan, and 48 hours of clinical expectation, surgical intervention was decided with diagnosis of high intestinal obstruction. Intraoperative finding was no intestinal rotation at all. Ladd´s technique was performed together with prophylactic appendectomy. Postoperative evolution was favorable. The rare presentation in adults motivates the presentation of this case.

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Introducción: Las complicaciones de la cirugía laparoscópica son una nueva entidad nosológica de fines del siglo XX, desde el inicio de la misma se han publicado múltiples complicaciones desde leves hasta graves, incluso con muerte de los pacientes. Objetivo: Presentación de un caso clínico. Caso Clínico: Paciente femenino de 48 años, se realizó colecistectomía laparoscópica en sanatorio privado con técnica americana sin complicaciones. 14 meses después consulta a nuestro hospital por dolores abdominales de tipo punzante, sin otro tipo de sintomatología, antecedente de 4 cesáreas y eventración media infraumbilical. Se solicita radiografía de abdomen y TAC. Se evidencia oblito quirúrgico, se decide la intervención quirúrgica, laparotomía media infraumbilical. Hallazgo: mandril de aguja de Verres. Buena evolución con alta a las 72 hs. Discusión: No se halló reporte sobre la posibilidad que el mandril de una aguja descartable se desprenda y quede dentro de la cavidad abdominal. Numerosos trabajos avalan la no utilización de la aguja de Verres para el neumoperitoneo, y aconsejan la utilización de la técnica de Hasson o mini-laparotomía, o los sistemas tipo optic-view para el ingreso de la cavidad.

Introduction: Complications of laparoscopic surgery are a new entity of the late twentieth century, from the beginning of the century have been published multiple complications, from mild to severe, even death of the patients. Objective: Presentation of a clinical case. Case report: Female aged 48, with a history of laparoscopic cholecystectomy in a private hospital 14 months ago with no complications, consulted our hospital with sharp abdominal pain, no other symptoms, with history of two caesarean sections and a infraumbilical eventration. CT and radiography is requested. Evidenced surgical oblito, deciding surgical intervention, infraumbilical laparotomy. Finding: Verres needle mandrel. Patient is discharged 72 hs later. Discusion: We found no report on the possibility that verres needle mandrel discards and remain in the abdominal cavity. Numerous studies support the non use of the Verres needle for the pneumoperitoneum and advise the use of Hasson technique or mini-laparoscopy, as well as the optic-view systems.

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Introducción: la Cirugía Ambulatoria es un modelo organizativo de asistencia quirúrgica, que permite tratar a pacientes bien seleccionados en forma efectiva, segura y eficiente, sin necesidad de contar con una cama de internación. La Colecistectomía laparoscópica es considerada actualmente el tratamiento de elección de la litiasis vesicular sintomática. El uso de la laparoscopía reduce la estadía hospitalaria y el tiempo de recuperación postoperatoria. Objetivo: evaluar la factibilidad, seguridad y beneficios de la realización de Colecistectomías Laparoscópica en Pacientes en una Unidad de Cirugía Ambulatoria. Material y Métodos: el registro de los pacientes durante el peroperatorio de Colecistectomías Laparoscópicas desde Enero del 2012 a Diciembre del 2014, en la Unidad de Cirugía Ambulatoria del Policlínico del Docente. Resultados: Del total de las cirugías, 623 fueron Colecistectomías Laparoscópicas de las cuales 519 se realizaron en forma programadas e ingresaron por U.C.A. y 104 por guardia. El tiempo promedio de permanencia en el área es de 8,25 hs. El índice de ambulatorización es del 83.3%. Conclusión: La realización de la Colecistectomía Laparoscópica en una modalidad ambulatoria en pacientes adecuadamente seleccionados, constituye una opción interesante para aumentar el número de colecistectomías en nuestro medio y modifica positivamente la percepción del paciente sobre su enfermedad y acelera su recuperación.

Introduction: Ambulatory Surgery is a surgical care organizationalmodel that allows selected patients to be treated effectively, safely and efficiently, without taken a bed of admission. Laparoscopic cholecystectomy is now considered the Gold Standard treatmentfor symptomatic gallstones. The laparoscopy surgery reduceshospital stay and postoperative time recovery.Objective: To evaluate the feasibility, safety and benefits ofperforming laparoscopic cholecystectomy in patients in a DaySurgery Unit (D.S.U). Material and Methods: the registration of patients during theperioperative Laparoscopic cholecystectomy from January 2012to December 2014, in the Day Surgery Unit from the Policinico delDocente.Results: Of all the surgeries, Laparoscopic cholecystectomy were623 of which 519 were performed as scheduled and entered byD.S.U. and 104 were admitted at the Urgencys Appartment. The average time spent in the area is 8.25 pm. Ambulatory index is83.3%. Conclusion: The realization of laparoscopic cholecystectomy inan outpatient based in properly selected patients, is an interestingmodel to increase the number of cholecystectomies in ourenvironment and positivel y change the perception of the patientabout his illness and recovery accelerates option.

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