RESUMO

ABSTRACT Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME and downstream of the constitutive arc gene cluster, and this situation may have an impact on its activation. Different relative expression behaviours are proven here for arcRACME and the arcACME operon compared to the constitutive ones. We also show that the artificially expressed recombinant ArcRACME protein binds to the promoter region of the arcACME operon; this mechanism can be related to a positive feedback model, which may be responsible for increased anaerobic survival of the USA300 clone during infection-related processes.

Assuntos

Humanos , Óperon/genética , Arginina/genética , Staphylococcus aureus/genética , Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Arginina/metabolismo , Staphylococcus aureus/metabolismo , Regulação Bacteriana da Expressão Gênica/genética , Sequências Repetitivas Dispersas/genética , Genes Bacterianos/genética

RESUMO

Staphylococcus aureus pandemic clone USA300 has, in addition to its constitutive arginine catabolism (arc) gene cluster, an arginine catabolism mobile element (ACME) carrying another such cluster, which gives this clone advantages in colonisation and infection. Gene arcR, which encodes an oxygen-sensitive transcriptional regulator, is inside ACME and downstream of the constitutive arc gene cluster, and this situation may have an impact on its activation. Different relative expression behaviours are proven here for arcRACME and the arcACME operon compared to the constitutive ones. We also show that the artificially expressed recombinant ArcRACME protein binds to the promoter region of the arcACME operon; this mechanism can be related to a positive feedback model, which may be responsible for increased anaerobic survival of the USA300 clone during infection-related processes.

Assuntos

Arginina/genética , Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Óperon/genética , Staphylococcus aureus/genética , Arginina/metabolismo , Regulação Bacteriana da Expressão Gênica/genética , Genes Bacterianos/genética , Humanos , Sequências Repetitivas Dispersas/genética , Staphylococcus aureus/metabolismo