RESUMO
The morphology and growth of the filamentous fungi are influenced by different factors as the culture conditions and the type of fermentative process. The production and secretion of metabolites by these organisms present a direct relationship with their morphology. The organization of the microtubules and actin in the cytoskeleton is determinant for both the fungal growth and morphology. In this context, this study aimed to analyze the expression of the ß-tubulin, F-actin, and glucan synthase in the A. niger mycelia obtained from submerged fermentation and biofilm fermentation through qPCR, as well as the analysis of the nucleus distribution in the hypha. Herein, we showed that ß-tubulin and the F-actin gene were more expressed in the biofilm condition, while the glucan synthase was in the submerged condition. No significant difference was observed in the nucleus distribution between the mycelia obtained from both the fermentative processes. In conclusion, the different morphologies observed for the mycelia from submerged fermentation and biofilm fermentation might be influenced by the differential modulation of genes that codify cytoskeleton proteins, which seems to be potentially regulated by mechanosensing during fungal contact with solid supports.
Assuntos
Actinas , Aspergillus niger , Biofilmes , Regulação Fúngica da Expressão Gênica , Micélio , Tubulina (Proteína) , Actinas/genética , Actinas/metabolismo , Aspergillus niger/genética , Aspergillus niger/metabolismo , Fermentação , Micélio/genética , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismoRESUMO
Foi identificado um gene denominado trdA9 em Aspergillus nidulans, o qual confere resistência aos fungicidas triadimefon e imazil. Este gne foi mapeado no grupo de ligaçäo VII, provavelmente alelo ao imaA4, e apresentou um carater dominante em diploide heterozigoto. Embora o grau de resistência mostrado pela linhagem mutante seja relativamente baixo, sugeriu-se que ele possa ser usado para desenvolver um sistema de transformaçäo. Além disto, foi mostrado que o triadimefon näo induz mutaçäo gênica no sistema metionina de AQ. nidulans