RESUMO
Sodium valproate (VPA) is a classic anticonvulsive, a histone deacetylase inhibitor, and a chromatin remodeling inducer. When injected into specimens of Triatoma infestans, a vector of Chagas disease, VPA affects the chromatin supraorganization of chromocenter heterochromatin in only a few cells of the Malpighian tubules. To test whether this result was explained by the inaccessibility of all of the organ's cells to the drug, we investigated the nuclear phenotypes and global acetylation of lysine 9 in histone H3 (H3K9ac) in Malpighian tubules cultivated in vitro for 1-24 h in the presence of 0.05 mM-1 mM VPA. The present results revealed that the chromatin decondensation event in the chromocenter body, which was detected only under low VPA concentrations up to a 4-h treatment, was not frequent during organ culture, similar to the results for injected insects. Cultivation of T. infestans Malpighian tubules in vitro for 24 h revealed inadequate for cell preservation even in the absence of the drug. Immunofluorescence signals for H3K9ac following VPA treatment showed a slightly increased intensity in the euchromatin, but were never detected in the chromocenter bodies, except with great intensity at their periphery, where the 18S rDNA is located. In conclusion, when VPA affects the chromocenter heterochromatin in this animal cell model, it occurs through a pathway that excludes a classic global H3K9ac mark. Investigation of nonhistone proteins associated with histone methylation marks is still required to further explain the differential response of T. infestans chromatin to VPA.
Assuntos
Eucromatina/metabolismo , Inibidores de Histona Desacetilases/farmacologia , Triatoma/efeitos dos fármacos , Ácido Valproico/farmacologia , Acetilação/efeitos dos fármacos , Animais , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Cromatina/efeitos dos fármacos , Cromatina/metabolismo , Heterocromatina/metabolismo , Túbulos de Malpighi/citologia , Túbulos de Malpighi/efeitos dos fármacos , Triatoma/citologiaRESUMO
The nuclear phenotypes of Malpighian tubule cells in fifth instar nymphs of Triatoma infestans, one of the most important vectors of Chagas disease, were studied following sequential shocks at 0 degrees C, separated by intervals of 8 h and 24 h at 30 degrees C, under conditions of moderate fasting and full nourishment. The insects pertained to colonies reared in the laboratory and originated from domestic specimens collected in the Brazilian states of São Paulo (north) and Minas Gerais (south). Since nuclear phenotypes in this species are affected by single cold shocks, it was expected that these phenotypes could also be changed by sequential shocks. Nuclear phenotypes indicative of mechanisms of cell survival (nuclear fusion and heterochromatin decondensation) and cell death (apoptosis and necrosis) were observed concomitantly in all the conditions tested. Nuclear fusion and heterochromatin decondensation were not found relevant for the presumed acquisition of the cold-hardening response in T. infestans. The decreased frequency of apoptosis and necrosis following sequential cold shocks including under fasting conditions, indicated that tolerance to sequential cold shocks occurred in T. infestans of the mentioned origin.
Assuntos
Núcleo Celular/ultraestrutura , Temperatura Baixa , Insetos Vetores/ultraestrutura , Túbulos de Malpighi/ultraestrutura , Fenótipo , Triatoma/ultraestrutura , Animais , Morte Celular , Núcleo Celular/fisiologia , Sobrevivência Celular , Células Epiteliais , Resposta ao Choque Térmico , Insetos Vetores/fisiologia , Túbulos de Malpighi/fisiologia , Triatoma/fisiologiaRESUMO
The nuclear phenotypes of Malpighian tubule cells in fifth instar nymphs of Triatoma infestans, one of the most important vectors of Chagas disease, were studied following sequential shocks at 0ºC, separated by intervals of 8 h and 24 h at 30ºC, under conditions of moderate fasting and full nourishment. The insects pertained to colonies reared in the laboratory and originated from domestic specimens collected in the Brazilian states of Säo Paulo (north) and Minas Gerais (south). Since nuclear phenotypes in this species are affected by single cold shocks, it was expected that these phenotypes could also be changed by sequential shocks. Nuclear phenotypes indicative of mechanisms of cell survival (nuclear fusion and heterochromatin decondensation) and cell death (apoptosis and necrosis) were observed concomitantly in all the conditions tested. Nuclear fusion and heterochromatin decondensation were not found relevant for the presumed acquisition of the cold-hardening response in T. infestans. The decreased frequency of apoptosis and necrosis following sequential cold shocks including under fasting conditions, indicated that tolerance to sequential cold shocks occurred in T. infestans of the mentioned origin
Assuntos
Animais , Fenótipo , Triatoma , Núcleo Celular , Temperatura Baixa , Insetos Vetores , Túbulos de Malpighi , Triatoma , Núcleo Celular , Sobrevivência Celular , Morte Celular , Resposta ao Choque Térmico , Células Epiteliais , Insetos Vetores , Túbulos de MalpighiRESUMO
The survival and molting incidence in Triatoma infestans, a vector of Chagas disease, were investigated following sequential shocks at 0 degrees C in fifth instar nymphs under moderate fasting and full nutritional conditions. The shocks were separated by intervals of 8 h and 24 h at 30 degrees C. The results indicated that in terms of insect survival, T. infestans is tolerant to a single cold shock at 0 degrees C even for 12 h, or to sequential cold shocks, regardless of the nutritional state of the specimens. In terms of molting rate, fasting enhanced the tolerance to sequential cold shocks, but did not exceed the tolerance acquired by fully-nourished specimens, except when cold shocks were separated by an 8 h interval at 30 degreesC. The protective action elicited by fasting was assumed to be additive to that induced by a single mild cold shock or sequential cold shocks. The cold-tolerance response of T. infestans may have favoured its survival in areas of South America with low temperatures, even considering that this species is predominantly associated with human habitats.
Assuntos
Temperatura Baixa , Muda/fisiologia , Triatoma/fisiologia , Animais , Ninfa/fisiologiaRESUMO
The survival and molting incidence in Triatoma infestans, a vector of Chagas disease, were investigated following sequential shocks at 0ºC in fifth instar nymphs under moderate fasting and full nutritional conditions. The shocks were separated by intervals of 8 h and 24 h at 30ºC. The results indicated that in terms of insect survival, T. infestans is tolerant to a single cold shock at 0ºC even for 12 h, or to sequential cold shocks, regardless of the nutritional state of the specimens. In terms of molting rate, fasting enhanced the tolerance to sequential cold shocks, but did not exceed the tolerance acquired by fully-nourished specimens, except when cold shocks were separated by an 8 h interval at 30ºC. The protective action elicited by fasting was assumed to be additive to that induced by a single mild cold shock or sequential cold shocks. The cold-tolerance response of T. infestans may have favoured its survival in areas of South America with low temperatures, even considering that this species is predominantly associated with human habitats
Assuntos
Animais , Triatoma , Temperatura Baixa , Muda , Taxa de Sobrevida , NinfaRESUMO
The nuclear phenotypes of Malpighian tubule epithelial cells of 5th instar male nymphs of the blood-sucking insect Panstrongylus megistus were studied immediately after a short (1 h) cold shock at 0o.C, and 10 and 30 days later. The objective was to compare the responses to a cold shock with those known to occur after hyperthermia in order to provide insight into the cellular effect of cold in this species. Nuclei which usually exhibited a conspicuous Y chromosome chromocenter were the most frequent phenotype in control and treated specimens. Phenotypes in which the heterochromatin was unravelled, or in which there was nuclear fusion or cell death were more abundant in the shocked specimens. Most of the changes detected have also been found in heat-shocked nymphs, except for nuclear fusion which generates giant nuclei and which appeared to be less effective or necessary than that elicited after heat shock. Since other studies showed that a short cold shock does not affect the survival of more than 14 percent of 5th instar nymphs of P. megistus with domestic habit and can induce tolerance to a prolonged cold shock, heat shock proteins proteins are probably the best candidates for effective protection of the cells and the insects from drastic damage caused by low temperature shocks
Assuntos
Animais , Masculino , Panstrongylus/genética , Temperatura Baixa/efeitos adversos , Resposta ao Choque Térmico/genética , Panstrongylus/fisiologia , Fenótipo , Túbulos de Malpighi/citologiaRESUMO
A descriptive, entomological and seroepidemiological study on Chagas disease was conducted in a place of recent occupation on the outskirts of Cochabamba, Bolivia: Avaroa/Primer de Mayo (population: 3.000), where the socio-economic level is low no control measures have been made available. Methods - The immunofluorescent antibody test (IFAT) was used for IgG and IgM anti-Trypanosoma cruzi antibodies in filter paper bloodspot eluates from 128 subject age, gender, birthplace, occupation, duration of residence and building materials used in their houses were recorded. Vectors were captured both in domestic and peridomestic environments. Results - Seropositive, 12,5 per cent (16/128): females, 15.1 per cent (11/73); males, 9.1 per cent (5/55). Average time of residence: 6.1 years for the whole population sample and 7.4 years for the seropositive subjects. Most houses had abobe walls (76.7 per cent, n = 30), galvanized iron rooves (86.7 per cent) and earthen floors (53.4 per cent) 80 per cent of the walls had crevices. One hundred forty seven specimens of Triatoma infestans were captured, of which 104 (70.7 per cent) were domestic, and 1 peridomestic Triatoma sordida. Precipitin host identification: birds, 67.5 per cent; humans, 27.8 per cent; rodents, 11.9 per cent; dogs, 8.7 per cent; cats, 1.6 per cent. House infestation and density indices were 53.3 and 493.0 respectively. We found 21 (14.3 per cent) specimens of T. infestans infected with trypanosomes, 18 (85.7 per cent) of which domestic environments. Discussion - The elements for the vector transmission of Chagas disease are present in Avaroa/Primer de Mayo and the ancient custom of keeping guinea pigs indoors adds to the risk of human infection. In neighboring Cochabamba, due to sub-standard quality control, contaminated blood transfusions are not infrequent, which further aggravates the spread of Chagas disease. Prompt action to check the transmission of this infection, involving additionally the congenital and transfusional modes of acquisition, is required.
Assuntos
Humanos , Feminino , Gatos , Cães , Animais , Criança , Pré-Escolar , Lactente , Pessoa de Meia-Idade , Adulto , Adolescente , Doença de Chagas/epidemiologia , Roedores , Triatoma/parasitologia , Trypanosoma cruzi/imunologia , População Urbana , Bolívia/epidemiologia , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Anticorpos Antiprotozoários/sangue , Estudos Soroepidemiológicos , Doença de Chagas/diagnóstico , Doença de Chagas/transmissão , Técnica Indireta de Fluorescência para Anticorpo , Vetores de DoençasRESUMO
Survival and molting incidence were studied after heat (40ºC) and cold (0ºC) shocks in specimens of Panstrongylus megistus with the aim of establishing its response to temperature stress under laboratory rearing conditions and to understand occasional changes in the biological characteristics of specimens captured in nature. The response to the thermal shocks was found to vary as a function of the temperature an duration of the shock, developmental phase and sex of the specimens, and in certain cases, the insect habit and nourishment conditions. P. megistus specimens were found to be less resistant to the heat shock assay than Triatoma infestans, another reduviid species. The short cold shock affected survival of P. megistus more than did the heat shock, survival of fully-nourished specimens being preferencial. The response of adults to the short cold shock was affected by sex, males being generally less resistant. The insect sylvatic habit was found to seldom affected the thermal shock response established for specimens with domestic habit. A decrease in molting frequency and sometimes a slowdown of the molting rate were found after the short heat and cold shocks, possibly promoted by changes in hormonal balance, and differing from patterns reported for T. infestans. The results indicate that no generalization should be made for different reduviid species in terms of the effects of temperature shocks.
Assuntos
Animais , Panstrongylus/crescimento & desenvolvimento , Temperatura Baixa , Temperatura Alta , Resposta ao Choque Térmico/fisiologia , SobrevivênciaRESUMO
Estuda o comportamento de triatomíneos no interior de ecótopos artificiais. Construiu-se um galinheiro experimental com paredes internas formadas por blocos de barros desmontáveis, abringando aves como fonte alimentar. O galinheiro foi subdividido em quatro áreas, duas destas separadas por telas e tijolos furados, sendo a menor o local de soltura dos triatomíneos e a maior o galinheiro propriamente dito. Dessa maneira buscou-se a coleta de informaçoes a respeito do deslocamento sobrevida e locais de abrigo de ninfas do 4§ estágio de Triatoma brasiliensis, na certeza de que os mesmos iriam se acomodar nas proximidades da fonte alimentar. As paredes do galinheiro foram desmontadas e pesquisadas mensalmente durante 6 meses e as ninfas encontradas, marcadas com diferentes cores de acordo com o mes da leitura e a posiçäo das mesmas nas áreas. Os resultados alcançados mostraram que 88,5 por cento das ninfas estavam engorgitadas e foram encontradas próximas ao local de repouso (poleiro) de fonte alimentar. Após a primeira alimentaçäo, näo abandonavam as proximidades da fonte. Os resultados alcançados apresentaram interesse epidemiológico e também interesse na área de controle dos vetores da doença de Chagas, tendo em vista as possíveis implicaçoes quanto aos procedimentos na aplicaçäo do inseticida.
Assuntos
Animais , Triatoma , Comportamento Animal , Comportamento Alimentar , Doença de ChagasRESUMO
Em continuaçäo a estudo anterior, relativo à prevalência por Trypanosoma cruzi dos triatomíneos capturados na regiäo administrativa de Campinas, SP, os autores apresentam dados do período de 1982-1986, acrescentando informaçöes sobre respastos sangüíneos realizados por 7.785 exemplares. Para tanto, foram utilizados os anti-soros: ave, marsupial, roedor e humano, através dos quais constataram o ecletismo alimentar de Panstrongylus megistus, espécie predominante na regiäo, cujas formas aladas säo encontradas com freqüência, infectadas por T. cruzi, nas casas habitadas. Desses, 14,78 por cento reagiram frente ao anti-soro humano. Com Rhodnius neglectus foi observada situaçäo assemelhada, mas com números menos expressivos. Em relaçäo ao Triatoma sordida, näo foi constatada infecçäo natural e tampouco sinais de ingestäo de sangue humano. Foi constatado acentuado aumento de T. arthurneivai nas casas, fruto da provável modificaçäo ocorrida no ambiente natural. No de t. arthuneivai nas casas, frutos da provável modificaçäo ocorrida no ambiente natural. No período, foi encontrado Microtiatoma borbai, detectado pela primeira vez no Estado de Säo Paulo. Ressaltam também a importância da "investigaçäo de foco" nas áreas em fase de vigilância
Assuntos
Humanos , Animais , Trypanosoma cruzi/isolamento & purificação , Triatominae/parasitologia , Insetos Vetores/parasitologia , Reservatórios de Doenças , Vigilância da População , Brasil/epidemiologia , Prevalência , Triatominae/fisiologia , Insetos Vetores/fisiologia , Ninfa/fisiologia , Ninfa/parasitologia , Comportamento Alimentar , Doença de Chagas/epidemiologia , Doença de Chagas/parasitologia , Reservatórios de Doenças/estatística & dados numéricosRESUMO
A sobrevivência e a ocorrência de mudas em espécies de Triatoma infestans foram estudadas num período de 30 dias após choques de temperatura. Foi demonstrado que choques hipertérmico e hipotérmico interferem nesses processos, na dependência da temperatura do choque, tempo de sua duraçäo, fase de desenvolvimento e sexo dos espécimes. Dentre as situaçöes experimentais utilizadas, o choque a 0§C por 12 h pareceu produzir a açäo mais deletéria, porém choques a 40§C e 0§
Assuntos
Animais , Masculino , Feminino , Temperatura , Triatoma/crescimento & desenvolvimento , Larva/crescimento & desenvolvimentoRESUMO
In paralel with several other epidemiologic and entomologic data of 19 Municipalities of Espírito Santo State, Brazil, the feeding pattern of 222 Triatoma vitticeps is studied through precipitin tests. Very high levels of natural infection with Trypanosoma cruzi are observed in adult insects, in contrast with the abscence or minimum degrees of infection among nymphs and human individuals. The precipitin tests showed the contact of the insects with multiple blood sources, chiefly human and birds, followed by rodents and marsupials. The data suggest that T. vitticeps in spite of being highly antropophilic, become infected by T. cruzi in sylvatic ambient and occasionally invade houses. The species doesn't seem to be - at least until now - a good vector in the domestic cycle of Chagas' disease. Several factors seem to be involved in this conclusion, mainly the low density of the insect in the houses, its hardness to coloniza them, its slowness concerning to suction and defecation and possibly its low susceptibility to different T. cruzi strains.
Assuntos
Humanos , Animais , Triatoma/parasitologia , Cardiomiopatia Chagásica/patologia , Doença de Chagas/parasitologia , Reduviidae , HemípterosRESUMO
Säo apresentados os resultados obtidos na investigaçäo entomo-epidemiológica realizada no estudo do primeiro caso humano autóctone de tripanossomíase americana no Estado do Acre (Brasil). A investigaçäo demonstrou ausência de domiciliaçäo triatomínea, ficando descartada totalmente a possibilidade de transmissäo congênita ou transfusional. Näo foi possível verificar se a transmissäo foi metaxênica através da invasäo domiciliar de barbeiro silvestre, ou se por via digestiva através de alimentos contaminados. Foram isoladas duas cepas de Trypanosoma cruzi, uma do caso humano chamada Acre-Humana (AH) e outra de Rhodnius robustus coletados em palmeiras uricuri (Attalea sp) nas proximidades da casa, chamada Acre-Silvestre (AS). Ficou comprovada a existência de correlaçäo entre os caracteres morfobiológicos e patogênicos das duas amostras estudadas: mostraram-se patogênicas para camundongos, infectando 100% dos animais, quer com formas metacíclicas de triatomíneos, quer com formas sanguícolas de doadores em fase aguda. A infecçäo dos camundongos nas duas amostras é grave com curto período pré-patente, parasitemia elevada e taxa de letalidade alta. Em ambas as cepas, em fase aguda, säo abundantes ninhos de amastigotas, principalmente no coraçäo e fígado. As amostras AH e AS conferem aos animais que sobrevivem boa resitência contra infecçöes pela amostra Y. Cultivaram-se facilmente em meios líquidos e semi-sólidos e infectaram experimentalmente seis espécies de triatomíneos. Os resultados comprovam mais uma vez a presença de focos naturais desta parasitose na regiäo.
Assuntos
Camundongos , Animais , Rhodnius/parasitologia , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificação , Doença de Chagas/transmissão , Reservatórios de Doenças , Trypanosoma cruzi/patogenicidade , Brasil , Vetores de DoençasRESUMO
Relata-se a pesquisa da tripanossomíase, na sua feição enzoótica doméstica, em áreas onde a transmissão pelo Triatoma infestans encontra-se interrompida há, pelo menos 6 (seis) anos. O encontro de cães positivos, embora com baixa prevalência (0,3%), foi associado à presença local de populações de Panstrongylus megistus e de marsupiais Didelphis, com franca tendência à domiciliação . O levantamento desse quadro enzoótico da zoonose, revelou-se assim como elemento útil na vigilância epidemiológica. (AUs
Assuntos
Ecologia , Habitação , TripanossomíaseRESUMO
A entrega à Superintendência de Controle de Endemias por um morador do Município do Espírito Santo do Pinhal, Estado de São Paulo (Brasil), de um inseto suspeito de barbeiro, encontrado na casa (quarto), sua correta identificação e exame, P. megistus infectado por Trypanosoma tipo cruzi, desencadeou uma operação de investigação de foco, realizada através de operações de campo e laboratório. Esses trabalhos evidenciaram a presença da enzootia chagásica naquela localidade, bem como, a possibilidade de T. cruzi chegar aos seus moradores. Tal não ocorrendo, entre outros fatores, em decorrência das atividades de controle em andamento. Foi destacado o valor da denúncia (notificação) feita pelo morador e relatadas atividades ligadas à investigação de foco.(AUs)
Assuntos
Triatoma/microbiologia , Triatoma/parasitologia , HabitaçãoRESUMO
Foram observados 53 exemplares do T. sórdida que chegaram à fase alada, oriundos de 110 ovos: os machos eram em número de 22 e as fêmeas 31. Os tempos médios de duração da fase de ninfa não foram estatísticamente diferentes entre os sexos, sendo 174,6 e 170,6 dias respectivamente para machos e fêmeas com 503 dias para estas e 284 para os machos. O período de oviposição correspondeu a 86,42% do tempo de fase alada das fêmeas, com a postura tendo a maior intensidade na primeira metade desta fase. Cada fêmea pôs em média 570,8 ovos, variando a postura de 0,68 a 1,97 ovos por dia. (AUs)