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1.
Methods Mol Biol ; 2759: 25-41, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38285136

RESUMO

Companies dedicated to the large-scale propagation of plant species are known as biofactories or agricultural biotechnology companies. Globally, there are a large number of biofactories (large-scale production) or plant tissue culture laboratories (small-scale production) in charge of supplying commercial propagules of plants of economic interest. Each biofactory implements technological developments such as temporary immersion (TIS) systems that allow them to reduce costs. This chapter analyzes some of the biofactories established globally, the main plant species propagated, and whether or not they implement the use of TIS.


Assuntos
Agricultura , Imersão , Biotecnologia , Laboratórios , Reprodução
2.
Methods Mol Biol ; 2759: 45-52, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38285137

RESUMO

The cultivation of vanilla (Vanilla planifolia) is of economic interest because vanillin is extracted from the fruits of this species. Vanillin is a natural flavoring highly valued in the food market. However, there is a short supply of propagules available for establishing commercial plantations and good-quality plants with phytosanitary certification. Plant tissue culture represents a viable option to supply large amounts of healthy plants to vanilla producers. In addition, the use of temporary immersion systems will allow commercial scale-up and the establishment of biofactories dedicated to in vitro vanilla propagation. This chapter describes a large-scale micropropagation protocol for vanilla using temporary immersion bioreactors (TIB).


Assuntos
Benzaldeídos , Vanilla , Imersão , Reatores Biológicos , Frutas
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