RESUMO

The S-specific pollen rejection response in Nicotiana depends on the interaction between S-RNase and a suite of SLF proteins. However, the biochemical pathway requires other essential proteins. One of them is the stigmatic protein NaStEP, which belongs to the Kunitz-type protease inhibitor family. Within the pollen tubes, NaStEP is a positive regulator of HT-B stability, likely inhibiting its degradation and, additionally, interacts with NaSIPP, a mitochondrial phosphate carrier. To gain a deeper understanding of the biochemical role of NaStEP in pollen rejection, we evaluated whether the activity of NaStEP as protease inhibitor is specific to a particular type of protease and whether it has the function of a voltage-dependent channel (VDC) blocker. Our findings indicate that, in vitro, NaStEP inhibits a subtilisin-like protease in an irreversible manner, but not other proteases, such as thermolysin and papain. Furthermore, we found that subtilisin processes the native NaStEP (24 kDa) into two lower molecular weight peptides of 21 and 14 kDa. Moreover, when we incubated NaStEP along with Xenopus leavis oocytes expressing the voltage-dependent potassium channel Kv 1.3, the current was blocked, indicating that NaStEP acts as a VDC blocker. These data allow us to propose NaStEP acts as a key molecule with two functions, one protecting HT-B from degradation by inhibiting a subtilisin-like protease and the second one by forming a complex with a mitochondrial VDC that could destabilize the mitochondria to trigger cell death, which would reinforce S-specific pollen rejection in Nicotiana.

Assuntos

RESUMO

Esta pesquisa foi realizada com o objetivo de se conhecer a variabilidade genética de 12 loci de microssatélites em galinhas crioulas Canela-Preta. Foram coletadas amostras de sangue de 118 galinhas crioulas Canela-Preta, provenientes de três municípios do estado do Piauí (Oeiras, Queimada Nova e Teresina). Após extração do DNA, foram utilizados marcadores para 12 loci de microssatélites: LEI0192, LEI0209, LEI0212, LEI0217, LEI0221, LEI0234, LEI0237, LEI0248, LEI0258, MCW0081, MCW0183 e MCW0213, que foram amplificados pela técnica de reação em cadeia da polimerase (PCR). Foram obtidos 408 alelos (somando os alelos dos 12 loci), com os fragmentos variando entre 50 e 460 pares de bases. O número de alelos variou de 15 (MCW0081) a 52 (LEI0212), com média de 31,5 alelos por locus. A média de heterozigosidade esperada e o conteúdo de informações polimórficas foram, respectivamente, 0,887 e 0,909. Foram observados desvios no equilíbrio de Hardy-Weinberg e valores positivos do índice de fixação com excesso de homozigotos. Os microssatélites utilizados mostraram-se polimórficos e podem ser usados para investigações genéticas em galinhas Canela-Preta. As galinhas dos plantéis avaliados apresentam grande variabilidade gênica, o que as qualifica como importante fonte de recursos genéticos e, consequentemente, faculta a utilização delas em programas de melhoramento genético animal.(AU)

The aim of this study was to analyze the genetic variability of twelve microsatellite loci in native Canela-Preta chickens. Blood samples were collected from 118 chickens of the breed from five properties in three cities (Oeiras, Queimada Nova and Teresina) of Piauí state. After the DNA extraction, markers were used for twelve microsatellite loci: LEI0192, LEI0209, LEI0212, LEI0217, LEI0221, LEI0234, LEI0237, LEI0248, LEI0258, MCW0081, MCW0183, and MCW0213 that were amplified by polymerase chain reaction technique (PCR). The results showed a total of 408 alleles (adding alleles from the 12 loci) with the fragments ranging between 50 and 460 base pairs, the number of alleles ranged from 15 (MCW0081) to 52 (LEI0212) with an average of 31,5 alleles per locus. The average expected heterozygosity and PIC were, respectively, 0.887 and 0.909. Deviations were observed in the Hardy-Weinberg equilibrium and positive values of the fixation index with excess of homozygotes. It is concluded that the used microsatellites are polymorphic and can, therefore, be used for genetic research in Canela-Preta chickens. The birds of the analyzed cores present great genetic variability, which qualifies them as an important source of genetic resources, which could be used for future animal breeding programs.(AU)

Assuntos

RESUMO

Esta pesquisa foi realizada com o objetivo de se conhecer a variabilidade genética de 12 loci de microssatélites em galinhas crioulas Canela-Preta. Foram coletadas amostras de sangue de 118 galinhas crioulas Canela-Preta, provenientes de três municípios do estado do Piauí (Oeiras, Queimada Nova e Teresina). Após extração do DNA, foram utilizados marcadores para 12 loci de microssatélites: LEI0192, LEI0209, LEI0212, LEI0217, LEI0221, LEI0234, LEI0237, LEI0248, LEI0258, MCW0081, MCW0183 e MCW0213, que foram amplificados pela técnica de reação em cadeia da polimerase (PCR). Foram obtidos 408 alelos (somando os alelos dos 12 loci), com os fragmentos variando entre 50 e 460 pares de bases. O número de alelos variou de 15 (MCW0081) a 52 (LEI0212), com média de 31,5 alelos por locus. A média de heterozigosidade esperada e o conteúdo de informações polimórficas foram, respectivamente, 0,887 e 0,909. Foram observados desvios no equilíbrio de Hardy-Weinberg e valores positivos do índice de fixação com excesso de homozigotos. Os microssatélites utilizados mostraram-se polimórficos e podem ser usados para investigações genéticas em galinhas Canela-Preta. As galinhas dos plantéis avaliados apresentam grande variabilidade gênica, o que as qualifica como importante fonte de recursos genéticos e, consequentemente, faculta a utilização delas em programas de melhoramento genético animal.(AU)

The aim of this study was to analyze the genetic variability of twelve microsatellite loci in native Canela-Preta chickens. Blood samples were collected from 118 chickens of the breed from five properties in three cities (Oeiras, Queimada Nova and Teresina) of Piauí state. After the DNA extraction, markers were used for twelve microsatellite loci: LEI0192, LEI0209, LEI0212, LEI0217, LEI0221, LEI0234, LEI0237, LEI0248, LEI0258, MCW0081, MCW0183, and MCW0213 that were amplified by polymerase chain reaction technique (PCR). The results showed a total of 408 alleles (adding alleles from the 12 loci) with the fragments ranging between 50 and 460 base pairs, the number of alleles ranged from 15 (MCW0081) to 52 (LEI0212) with an average of 31,5 alleles per locus. The average expected heterozygosity and PIC were, respectively, 0.887 and 0.909. Deviations were observed in the Hardy-Weinberg equilibrium and positive values of the fixation index with excess of homozygotes. It is concluded that the used microsatellites are polymorphic and can, therefore, be used for genetic research in Canela-Preta chickens. The birds of the analyzed cores present great genetic variability, which qualifies them as an important source of genetic resources, which could be used for future animal breeding programs.(AU)

Assuntos

RESUMO

Understanding how to improve decision makers' use of scientific information across their different scales of management is a core challenge for narrowing the gap between science and conservation practice. Here, we present a study conducted in collaboration with decision makers that aims to explore the functionality of the mechanisms for scientific input within the institutional setting of the National Protected Area Network of Peru. First, we analyzed institutional mechanisms to assess the scientific information recorded by decision makers. Second, we developed two workshops involving scientists, decision makers and social actors to identify barriers to evidence-based conservation practice. Third, we administered 482 questionnaires to stakeholders to explore social perceptions of the role of science and the willingness to collaborate in the governance of protected areas. The results revealed that (1) the institutional mechanisms did not effectively promote the compilation and application of scientific knowledge for conservation practice; (2) six important barriers hindered scientific input in management decisions; and (3) stakeholders showed positive perceptions about the involvement of scientists in protected areas and expressed their willingness to collaborate in conservation practice. This collaborative research helped to (1) identify gaps and opportunities that should be addressed for increasing the effectiveness of the institutional mechanisms and (2) support institutional changes integrating science-based strategies for strengthening scientific input in decision-making. These insights provide a useful contextual orientation for scholars and decision makers interested in conducting empirical research to connect scientific inputs with operational aspects of the management cycle in other institutional settings around the world.

Assuntos

RESUMO

The most common reason for non-adherence to medication among older adults is forgetfulness. Contextual cues, such as daily routines, serve as implicit situational information that increases the retrieval process of the intended action. The main contribution is an overview of the process and the technical details of Ambient Computing displays we developed to help seniors use contextual cues to remember actions associated with medication intake (i.e. remember to take medications or remember having taken them earlier). Through a qualitative study, we obtained evidence about the potential of our technological approach to make seniors more responsible and independent for taking medications.

Las razones más comunes para que adultos mayores no se apeguen a la medicación es el olvido. Las rutinas de vida diaria sirven como pistas contextuales que mejoran el proceso cognitivo relacionado con recordar realizar una acción planeada. Nuestro propósito es presentar el proceso de desarrollo, así como detalles técnicos, de Sistemas de Cómputo Ambiental que proveen pistas contextuales al adulto mayor para ayudarle a recordar acciones de su medicación (e.g., recordar medicarse o recordar que se medicaron). Mediante un estudio cualitativo, obtuvimos evidencia del potencial de nuestra tecnología para que el adulto mayor sea más responsable e independiente para medicarse.

RESUMO

An inductively coupled plasma optical emission spectrometry quantification method for the determination of Al, Ca, Cr Cu, Fe, Mn, Mg, Ni, Zn, Ba, K, In and Co in lead zirconate-titanate (PZT) ceramics, modified with strontium and chromium, was developed. Total digestion of ceramics was achieved with a HNO(3), H(2)O(2) and HF (ac) mixture by using a microwave furnace. The sensitivity of the net signal intensity respect to radiofrequency power (P) and nebulizer argon flow (F(N)) variations was strongly dependent of the total excitation energy of line (TEE). For lines with TEE near metastable atoms and ions of argon, an increment of the sensitivities to P and F(N) variation was observed. At robust plasma conditions the matrix effect was reduced for all matrices and analytes considered. The precision of analysis ranged from 3 to 13%, whereas the analytes recoveries in the spiked samples varied, mostly, from 90 to 110%. The detection limits of studied elements were from 0.004 to 10 mg kg(-1).

Assuntos

RESUMO

Mango (Mangifera indica L.) stem bark aqueous extract (MSBE) is a new natural product with antioxidant, anti-inflammatory and immunomodulatory effects known by the brand name of its formulations as Vimang. Previously, the oral toxicity studies of the extract showed a low toxicity potential up to 2000 mg/kg. This work reports the results about teratogenic and genotoxicologic studies of MSBE. For embryotoxicity study, MSBE (20, 200, or 2000 mg/kg/day) was given to Sprague-Dawley rats by gavage on days 6-15 of gestation. For genotoxicity, MSBE was administered three times during 48 h to NMRI mice. Cyclophosphamide (50 mg/kg) was used as a positive control. No maternal or developmental toxicities were observed when the rats were killed on day 20th. The maternal body-weight gain was not affected. No dose-related effects were observed in implantations, fetal viability or external fetal development. Skeletal and visceral development was similar among fetuses from all groups. No genotoxicity was observed in bone marrow erythrocytes and liver cells after administration. MSBE appears to be neither embryotoxic nor genotoxic as measured by bone marrow cytogenetics in rodents.

Assuntos

RESUMO

D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax with cholesterol-lowering and antiplatelet effects. In order to further characterize the developmental toxicity during the treatment period from late gestation up to weaning of the offspring, pregnant females received 0 (control), 500, and 1,000 mg/kg/day D-003 daily by oral gavage beginning at day 15 of pregnancy and through gestation until day 21 postpartum. Maternal clinical signs, body weight, and food intake were measured at regular intervals during gestation and lactation. Live pups were weighed, sexed, and examined for developmental signs. One female and male of each litter were randomly selected to evaluate the reproductive potential. There were no spontaneous or dose-related maternal deaths during the course of this study. The general health and behavioral condition of offspring was good in all groups. No significant differences among groups were found in comparisons of litter size, survival through the weaning period, sex ratio, and male and female weights. This peri- and postnatal study conducted with D-003 in rats indicated that treatment of the dam during late gestation and lactation did not show adversely effects on reproductive performance or fetal development over two generations.

Assuntos

RESUMO

D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax with cholesterol-lowering properties. D-003 given orally (500 and 1000 mg/kg/day) to female rats for 15 days prior to mating, through mating and gestation to day 21 of lactation and male rats for 4 weeks prior and during mating did not induce toxic effects on reproduction. There were no significant reductions in the number of animals that conceived, in the numbers of pups born to those that did conceive, in the numbers of pups that survived until weaning, and in their body weights at weaning. Drug-treated and control groups' offspring were comparable in growth, physical and behavioral development, spontaneous activity and reproductive performance. Pregnant New Zealand rabbits were given D-003 as oral doses of 500 and 1000 mg/kg/day on days 6 through 18 of gestation without any evidence of embryotoxicity or teratogenicity. The no-observed-effect dose in these two experimental studies was 1000 mg/kg/day. After assessment of the potential of high doses of D-003 to act on developing embryo and reproduction process, no evidence supports the conclusion that D-003 is a reproductive and developmental toxicant/teratogen.

Assuntos

RESUMO

D-003 is a mixture of long-chain fatty acids isolated and purified from sugar cane wax, the major component of which is 1-octacosanoic acid and which possesses effective antiplatelet, antithrombotic and cholesterol-lowering effects. D-003 was suspended in 1% acacia gum solution, and given daily by gavage to rats at dose levels of 5, 100 and 1000 mg/kg/day on days 6 through 15 of gestation. Cyclophosphamide, serving as a positive control, was given at the dose of 50 mg/kg/day on day 15 of gestation. Evidence of maternal or developmental toxicity was not observed in the groups treated with D-003. Maternal clinical signs of toxicity were not observed and the analysis of initial body weight and the body weight gain during the treatment period was comparable among the groups treated with D-003 and control. As expected, cyclophosphamide caused both embryotoxic and teratogenic effects in rats. Meanwhile, no adverse effects on reproductive performance, or on embryonic or fetal development, including visceral and skeletal examination, were seen in any of the groups administered D-003. It is concluded that D-003 administered up to 1000 mg/kg/day did not induce any evidence of developmental toxicity.

Assuntos

RESUMO

D-002 is a mixture of higher aliphatic alcohols, primarily isolated and purified from beeswax, that has moderate anti-inflammatory and effective anti-ulcer activity. In the present study the developmental toxicity in Sprague-Dawley rats and New Zealand White rabbits was evaluated. Doses of 100, 320 and 1000 mg kg(-1) were administered once daily by gavage to pregnant females on gestation days 6-15 (rats) or 6-18 (rabbits). Reproductive indices were determined and foetuses were examined for external, visceral and skeletal malformations. There was no indication of embryotoxicity, fetotoxicity or teratogenicity in any of the treated groups. It is concluded that D-002 is devoid of teratogenic or embryotoxic effects in these strains.

Assuntos

RESUMO

The effects of Policosanol, a newly developmented hypocholesterolemic drug administered during the perinatal and postnatal periods, were studied in Sprague-Dawley rats. This compound was administered orally to female rats at dose levels of 0 (control), 5, 50, and 500 mg/kg/day, from day 15 of pregnancy to day 21 after parturition. The animals were allowed to deliver and their offspring were examined for postnatal growth and development. No signs of toxic effects related to the test material were observed in the dams F0 during pregnancy and lactation. No adverse effects were observed on the postnatal growth, behaviours, or reproductive ability of pups F1. The physical and sensorial development of pups F2 was also normal. These results confirm that Policosanol does not affect the reproductive performance or fetal/neonatal development.

Assuntos

RESUMO

The hypocholesterolaemic drug policosanol was administered to Sprague-Dawley rats of both sexes throughout three successive generations at concentrations of 0, 5, 50 and 500 mg/kg bw/day by gavage. For each generation two litters were reared until they were at least 3 weeks old. No clinical signs which could be related to the administration of the test substance were observed in the F0, F1b and F2b parents. There were no differences among groups in the number of animals that conceived, the number of pups born live or dead, the rate of male to female pups, the number of pups that survived until weaning and the pups' body weights through the lactancy. The following test showed no treatment-related effects on F3b offspring: righting on a surface, air righting, corneal, pirmal and pain reflexes, auditory startle and visual placing. The results of the present study did not demonstrate any deleterious effects on the fertility, reproductive performance or development of rats administered policosanol at levels of up to 500 mg/kg bw/day over three successive generations.

Assuntos

RESUMO

The prevalence of risk factors for coronary atherosclerosis were studied in two population samples, Northeast (Posadas, n = 498) and South (Viedma, C. Rivadavia and Cipolletti, n = 652) of 20 years and older, males and females. The diet in the Northeast (n = 102) contained more monounsaturated acids and polyunsaturated acids than the one in the South (n = 62), 9.5 +/- 4.1 vs. 8.1 +/- 3.5% TCV (Total Caloric Value) (P < 0.02) and 8.1 +/- 4.1 vs. 6.2 +/- 3.0% TCV (P < 0.001) respectively. The P/S relationship was greater in the Northeast, 1.02 +/- 0.44 vs. 0.85 +/- 0.50 (P < 0.001). Total cholesterol (TC) in the Northeast was less than in the South, in males 176 +/- 41 vs. 213 +/- 43 mg/dl (P < 0.001); CLDL (LDL cholesterol) 109 +/- 37 vs. 141 +/- 41 mg/dl (P < 0.001). The most frequent risk factors in the South vs. Northeast (males) were: TC > or = 240 mg/dl, 26.7% vs. 9.5% (P < 0.001); LDL-C > or = 160 mg/dl, 30.3% vs. 10.9% (P < 0.001); Cig > or = 10/d (equal or more than 10 cigarettes per day), 30.0% vs. 16.4% (P < 0.001). The hypertension prevalence (HTA, 160/95), in males, was higher in the Northeast than in the South, 23.7% vs. 11.5% (P < 0.001). BMI > 27 Kg/m2 was higher in the women of Northeast than in the South, 38.4% vs. 24.2% (P < 0.001). In the males of the Northeast, the combination Cig > or = 10/d and HTA, 4.1 vs 0.9% was more common; in the South Cig > or = 10/d and LDL-C > or = 160 mg/d, 8.2% vs. 1.8% (P < 0.001) was more common. The differences in the prevalence of the risk factors between the population samples indicate the need to plan the prevention of coronary atherosclerosis locally.

Assuntos

RESUMO

The present studies evaluated the teratogenic potential and reproductive toxicity of Policosanol, a new hypocholesterolemic drug. Policosanol was administered by oral gavage to Sprague-Dawley rats and New Zealand White rabbits during the period of organogenesis at dosages up to 500 and 1,000 mg/kg/day, respectively. There was no evidence of teratogenicity or any other embryonal toxicity. In a fertility and reproductive study female rats were treated with Policosanol by oral gavage at dosages up to 500 mg/kg/day, 2 weeks prior to mating and throughout mating and pregnancy to day 21 of lactation. Males were given treatment 60 days before and during mating. Reproductive parameters of mothers were normal. There was no evidence that treatment affected the survival, postnatal growth, or behavior of the offspring. No maternal treatment-related adverse side effects occurred in these studies. It is concluded that Policosanol was not teratogenic in either rats or rabbits, nor did it induce reproductive toxicity in rats.

Assuntos

RESUMO

The prevalence of risk factors for coronary atherosclerosis were studied in two population samples, Northeast (Posadas, n = 498) and South (Viedma, C. Rivadavia and Cipolletti, n = 652) of 20 years and older, males and females. The diet in the Northeast (n = 102) contained more monounsaturated acids and polyunsaturated acids than the one in the South (n = 62), 9.5 +/- 4.1 vs. 8.1 +/- 3.5

TCV (Total Caloric Value) (P < 0.02) and 8.1 +/- 4.1 vs. 6.2 +/- 3.0

TCV (P < 0.001) respectively. The P/S relationship was greater in the Northeast, 1.02 +/- 0.44 vs. 0.85 +/- 0.50 (P < 0.001). Total cholesterol (TC) in the Northeast was less than in the South, in males 176 +/- 41 vs. 213 +/- 43 mg/dl (P < 0.001); CLDL (LDL cholesterol) 109 +/- 37 vs. 141 +/- 41 mg/dl (P < 0.001). The most frequent risk factors in the South vs. Northeast (males) were: TC > or = 240 mg/dl, 26.7

vs. 9.5

(P < 0.001); LDL-C > or = 160 mg/dl, 30.3

vs. 10.9

(P < 0.001); Cig > or = 10/d (equal or more than 10 cigarettes per day), 30.0

vs. 16.4

(P < 0.001). The hypertension prevalence (HTA, 160/95), in males, was higher in the Northeast than in the South, 23.7

vs. 11.5

(P < 0.001). BMI > 27 Kg/m2 was higher in the women of Northeast than in the South, 38.4

vs. 24.2

(P < 0.001). In the males of the Northeast, the combination Cig > or = 10/d and HTA, 4.1 vs 0.9

was more common; in the South Cig > or = 10/d and LDL-C > or = 160 mg/d, 8.2

vs. 1.8

(P < 0.001) was more common. The differences in the prevalence of the risk factors between the population samples indicate the need to plan the prevention of coronary atherosclerosis locally.

RESUMO

Se realizó un estudio clínico, cardiológico, bioquímico y nutricional para determinar factores de riesgo. Se compararon individuos de un área rural, Comi-Có (N=92), en su mayoría descendientes de Mapuches, con un grupo urbano de Viedma (N=94). Se analizó la carne ovina, caprina y equina de la zona rural. La brasa intramuscular equina fue 1,16g//, con 34,5// de AG poliinsaturados (linolénico 11,9//). La dieta rural fue diferente de la urbana (p < 0,001): calorías totales 3420 ñ 1027 vs 2110 ñ 654 Kcal, hidratos carbono 40 ñ 8 vs 55 ñ 10//, lípidos 33 ñ 5 vs 24 ñ 8//, proteínas 28 ñ 9 vs 20 ñ 6//, colesterol 1021 ñ 380 vs 385 ñ 156 mg/d. Los rurales tenían tendencia a la patología biliar. Los varones de Comi-Có vs Viedma tenían C-HDL 53 ñ 13 vs 47 ñ 9 mg/dl (p < 0,05) y no se observaron diferencias en las medidas para CT, CLDL, TG, Apo B y Acido Urico. La frecuencia de: CT ò 240 mg/dl, CLDL, ò 160 mg/dl y cigarrillos ò 10/día pareció ser menor en los individuos rurales. En varones de Videma vs Como-Có con un factor principal se halló 45// vs 24// (p < 0,05). La proporción con dos factores fue baja. No se hallaron individuos con tres factores. Puede concluirse para estas muestras : 1) La dieta rica en carnes rojas del área rural patagónica no aumentó los niveles lipídicos en sangre; la frecuencia de los factores e indicadores de riesgo en la muestra rural no fue mayor que en la urbana. 2) El estilo de vida y factores étnicos podrían condicionar el efecto de la dieta sobre los lípidos sanguíneos (AU)

Assuntos

RESUMO

Se realizó un estudio clínico, cardiológico, bioquímico y nutricional para determinar factores de riesgo. Se compararon individuos de un área rural, Comi-Có (N=92), en su mayoría descendientes de Mapuches, con un grupo urbano de Viedma (N=94). Se analizó la carne ovina, caprina y equina de la zona rural. La brasa intramuscular equina fue 1,16g//, con 34,5// de AG poliinsaturados (linolénico 11,9//). La dieta rural fue diferente de la urbana (p < 0,001): calorías totales 3420 ñ 1027 vs 2110 ñ 654 Kcal, hidratos carbono 40 ñ 8 vs 55 ñ 10//, lípidos 33 ñ 5 vs 24 ñ 8//, proteínas 28 ñ 9 vs 20 ñ 6//, colesterol 1021 ñ 380 vs 385 ñ 156 mg/d. Los rurales tenían tendencia a la patología biliar. Los varones de Comi-Có vs Viedma tenían C-HDL 53 ñ 13 vs 47 ñ 9 mg/dl (p < 0,05) y no se observaron diferencias en las medidas para CT, CLDL, TG, Apo B y Acido Urico. La frecuencia de: CT ò 240 mg/dl, CLDL, ò 160 mg/dl y cigarrillos ò 10/día pareció ser menor en los individuos rurales. En varones de Videma vs Como-Có con un factor principal se halló 45// vs 24// (p < 0,05). La proporción con dos factores fue baja. No se hallaron individuos con tres factores. Puede concluirse para estas muestras : 1) La dieta rica en carnes rojas del área rural patagónica no aumentó los niveles lipídicos en sangre; la frecuencia de los factores e indicadores de riesgo en la muestra rural no fue mayor que en la urbana. 2) El estilo de vida y factores étnicos podrían condicionar el efecto de la dieta sobre los lípidos sanguíneos

Assuntos

RESUMO

To determine risk factors, a clinical, cardiologic, biochemical and nutritional study was carried out. A sample of the rural population of Comi-Co (N = 92), which included descendants of Mapuche indians, was compared with a sample of the urban population of Viedma (N = 94). Sheep, goat and horse meat from the rural district was analyzed. In horse meat, intramuscular fat was 1.16 g%, with 34.5% of polyunsaturated fatty acids including linolenic (18:3 w3) 11.9%, linoleic (18:2 w6) 21.0%. The diet intake of the rural population was very different from that of the urban population (p < 0.001). It contained 3420 +/- 1027 vs 2110 +/- 654 kcal, carbohydrates 40 +/- 8 vs 55 +/- 10%, lipids 33 +/- 5 vs 24 +/- 8%, proteins 28 +/- 6 vs 20 +/- 6%, cholesterol 1021 +/- 380 vs 385 +/- 156 mg/day. The rural population showed a tendency to suffer cholelithiasis. Comi-Co vs Videma males had a CHDL 53 +/- 13 vs 47 +/- 9 mg/dl (p < 0.05) and no significant differences were found between both groups when it came to blood glucose, uric acid, triglycerides and Apo B. The frequency of individuals with CT > or = 240 mg/dl, CLDL > or = 160 mg/dl, cigarettes > or = 10/day seems to be less in the rural district. In Viedma vs Comi-Co males, with any one primary factor there was a ratio of 45% vs 24% (p < 0.05). The proportion of males with two risk factors was low. No individuals with three risk factors were found.(ABSTRACT TRUNCATED AT 250 WORDS)

Assuntos

RESUMO

To determine risk factors, a clinical, cardiologic, biochemical and nutritional study was carried out. A sample of the rural population of Comi-Co (N = 92), which included descendants of Mapuche indians, was compared with a sample of the urban population of Viedma (N = 94). Sheep, goat and horse meat from the rural district was analyzed. In horse meat, intramuscular fat was 1.16 g

, with 34.5

of polyunsaturated fatty acids including linolenic (18:3 w3) 11.9

, linoleic (18:2 w6) 21.0

. The diet intake of the rural population was very different from that of the urban population (p < 0.001). It contained 3420 +/- 1027 vs 2110 +/- 654 kcal, carbohydrates 40 +/- 8 vs 55 +/- 10

, lipids 33 +/- 5 vs 24 +/- 8

, proteins 28 +/- 6 vs 20 +/- 6

, cholesterol 1021 +/- 380 vs 385 +/- 156 mg/day. The rural population showed a tendency to suffer cholelithiasis. Comi-Co vs Videma males had a CHDL 53 +/- 13 vs 47 +/- 9 mg/dl (p < 0.05) and no significant differences were found between both groups when it came to blood glucose, uric acid, triglycerides and Apo B. The frequency of individuals with CT > or = 240 mg/dl, CLDL > or = 160 mg/dl, cigarettes > or = 10/day seems to be less in the rural district. In Viedma vs Comi-Co males, with any one primary factor there was a ratio of 45

vs 24

(p < 0.05). The proportion of males with two risk factors was low. No individuals with three risk factors were found.(ABSTRACT TRUNCATED AT 250 WORDS)