RESUMO
Extracts of Hibiscus sabdariffa L. (commonly called Rosselle or "Jamaica flower" in Mexico) have been shown to have antibiotic and antivirulence properties in several bacteria. Here, an organic extract of H. sabdariffa L. is shown to inhibit motility in Salmonella enterica serovars Typhi and Typhimurium. The compound responsible for this effect was purified and found to be the hibiscus acid. When tested, this compound also inhibited motility and reduced the secretion of both flagellin and type III secretion effectors. Purified hibiscus acid was not toxic in tissue-cultured eukaryotic cells, and it was able to reduce the invasion of Salmonella Typhimurium in epithelial cells. Initial steps to understand its mode of action showed it might affect membrane proton balance.
Assuntos
Antibacterianos/farmacologia , Citratos/farmacologia , Flagelos/fisiologia , Flores/química , Hibiscus/química , Extratos Vegetais/farmacologia , Salmonella enterica/efeitos dos fármacos , Flagelos/efeitos dos fármacosRESUMO
Background: Infections caused by Candida species and Staphylococcus aureus are associated with biofilm formation. C. albicans-S. aureus interactions are synergistic due to the significant increase in mixed biofilms and improved resistance to vancomycin of S. aureus. C. glabrata and S. aureus both are nosocomial pathogens that cause opportunistic infections in similar host niches. However, there is scarce information concerning the interaction between these last microorganisms. Results: The relationship between C. glabrata and S. aureus was evaluated by estimating the viability of both microorganisms in co-culture of planktonic cells and in single and mixed biofilms. An antagonistic behavior of S. aureus and their cell-free bacterial supernatant (CFBS) toward C. glabrata, both in planktonic form and in biofilms, was demonstrated. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM) images showed yeast cells surrounded by bacteria, alterations in intracytoplasmic membranes, and non-viable blastoconidia with intact cell walls. Concomitantly, S. aureus cells remained viable and unaltered. The antagonistic activity of S. aureus toward C. glabrata was not due to cell-to-cell contact but the presence of CFBS, which causes a significant decrement in yeast viability and the formation of numerous lipid droplets (LDs), reactive oxygen species (ROS) accumulation, as well as nuclear alterations, and DNA fragmentation indicating the induction of an apoptotic mechanism. Conclusion: Our results demonstrate that the S. aureus CFBS causes cell death in C. glabrata by an apoptotic mechanism.
RESUMO
Se presenta el caso de una paciente portadora de una aspergilliosis pulmonar. Dicha afección se implantó en una caverna detergida como consecuencia de una tuberculosis pulmonar. Luego de analizar las características generales del aspergillus, se describen sus distintas manifestaciones clínicas insistiendo en la forma cavitaria. Se señalan aspectos del tratamiento médico y quirúrgico, poniendo énfasis en la oportunidad y tipo de intervención a indicar (AU)
Assuntos
Adulto , Humanos , Feminino , Aspergilose/etiologia , Pneumopatias Fúngicas/etiologia , Tuberculose Pulmonar/complicaçõesRESUMO
Se presenta el caso de una paciente portadora de una aspergilliosis pulmonar. Dicha afección se implantó en una caverna detergida como consecuencia de una tuberculosis pulmonar. Luego de analizar las características generales del aspergillus, se describen sus distintas manifestaciones clínicas insistiendo en la forma cavitaria. Se señalan aspectos del tratamiento médico y quirúrgico, poniendo énfasis en la oportunidad y tipo de intervención a indicar