RESUMO
Physical activity results in oxidative stress, as evidenced by the increased production of reactive oxygen, nitrogen species, and inflammatory mediators. The management of these components is instrumental for antioxidant adaptation to exercise and post-exercise recovery. Therefore, the present report aims to study the antioxidant response to two types of exercise (a 2000 m run and a burpee test) in healthy volunteers after a long period of inactivity (1-2 months). Antioxidant enzyme activities and oxidative stress markers (protein carbonyls and malondialdehyde content) were measured in neutrophils, peripheral blood mononuclear cells, and plasma. These parameters were determined under basal conditions and immediately post-exercise. Compared to those in basal state, neutrophil superoxide dismutase (28.3 vs. 22.9 pkat/109 cells), glutathione peroxidase (147.5 vs. 120.1 nkat/109 cells), and catalase (106.3 vs. 57.9 k/109 cells) were activated significantly (p < 0.05) after the burpee test. Peripheral blood mononuclear cells exhibited only significant (p < 0.05) catalase activation (113.6 vs. 89.4 k/109 cells) after the burpee test. Other enzymes, such as glutathione reductase and myeloperoxidase, tended to increase post-exercise, although the differences from baseline were not significant. Finally, compared to basal conditions, the protein carbonyl (24.5 vs. 14.5 mmol/L) and malondialdehyde (39.6 vs. 18.3 mmol/L) contents increased significantly (p < 0.05) in neutrophils and in plasma (115.1 vs. 97.8 and 130.2 vs. 123.4 µmol/L, respectively) after the burpee test. In conclusion, high-intensity exercise seems to induce immediate oxidative stress in inactive individuals, and the acute antioxidant response was slightly greater after the burpee test than after the 2000 m run. Glutathione-dependent antioxidant systems are activated immediately as protective mechanisms.
RESUMO
Oxidative stress is associated with playing soccer. The objective of the present report was to study the influence of different polyphenolic antioxidant-rich beverages in five-a-side/futsal players. The study was performed with a no supplemented control group (CG) and two supplemented groups with an almond-based beverage (AB) and the same beverage fortified with Lippia citriodora extract (AB + LE). At day 22, participants played a friendly futsal game. Blood extractions were performed at the beginning of intervention (day 1), before and after match (day 22) to determine oxidative stress markers and antioxidant enzyme activities in plasma, neutrophils and peripheral blood mononuclear cells (PBMCs). Malondialdehyde increased significantly in controls after the match in neutrophils, PBMCs and plasma compared to pre-match. Protein carbonyls also increased after the match in plasma in CG. In addition, malondialdehyde levels in neutrophils were significantly lower in the supplemented groups compared to controls. Post-match samples showed significant increases in neutrophil antioxidant activities in CG. Supplemented groups displayed variable results regarding neutrophil antioxidant activities, with superoxide dismutase activity significantly lower than in controls. Finally, post-match myeloperoxidase activity increased significantly in controls compared to pre-match and supplemented groups. In conclusion, polyphenolic antioxidant and anti-inflammatory supplements could be instrumental for optimal recovery after high intensity futsal games.
Assuntos
Futebol , Humanos , Antioxidantes/metabolismo , Leucócitos Mononucleares/metabolismo , Malondialdeído , Estresse Oxidativo/fisiologia , Polifenóis/farmacologia , Futebol/fisiologiaRESUMO
The aim of the present report was to evaluate the inflammatory response to a 2000-m running test considering neutrophil myeloperoxidase as an inflammatory marker, and to verify if supplements rich in antioxidants could modulate Post-test antioxidant and anti-inflammatory responses. To this end, a 21-day homogenization period was carried out with three groups: a control group, a supplemented group taking an almond beverage enriched with vitamins C and E and a third group consuming the same beverage but enriched with Lippia citriodora extract. At the end of this period, participants performed a 2000-m run, and blood samples were obtained the day before and immediately after the running test. Plasma and neutrophils were isolated. As a result, plasma creatine kinase and myoglobin increased, indicating Post-test muscle damage. Plasma oxidative markers were increased in all groups, except in the group supplemented with the almond beverage. Neutrophil antioxidant enzymes were significantly increased only in the control group, suggesting an antioxidant effect of the supplements provided in the other groups. Myeloperoxidase activity was significantly increased after the test in the control group, while increased enzyme levels were detected in plasma of the supplement groups. Therefore, antioxidant consumption seems to favour myeloperoxidase release. The connection of this observation with post-exercise recovery will require further investigation.
RESUMO
Skinfold determinations from different body parts are occasionally used in prediction equations to calculate fatness. This is a critical parameter that conditions optimal sport performance. Reference values of skinfolds in top athletes do not exist. Therefore, the main objective of this study is to present skinfold reference values from a large population of athletes. Triceps, subscapular, suprailiac, abdominal, thigh and medial calf skinfolds were measured according to ISAK (International Society for Advancement of Kinanthropometry) norms in 2500 athletes. Six skinfold sums as well as partial sums from different body segments (upper, trunk and lower) were applied. Data show that the highest skinfold values were observed for thigh calf and abdominal, being the subscapular the lowest. Women presented higher skinfold values than men. Concerning partial sums, trunk and lower body segments presented the highest scores. Subjects practicing hockey, handball, indoor soccer, rowing, scuba diving and table tennis displayed the highest fat percents. In conclusion, fatty mass calculation in athletes should consider the six skinfold sum. In addition, body fat tends to be localized in the trunk and lower body segments in athletes and varies according to gender and sport discipline...
Las determinaciones de los pliegues subcutáneos de diferentes zonas corporales son utilizadas ocasionalmente en ecuaciones predictivas para calcular la masa grasa. Éste es un parámetro crítico que condiciona el óptimo rendimiento deportivo. No existen valores de referencia de pliegues subcutáneos en atletas de élite. Por tanto, el principal objetivo de este estudio es presentar valores de referencia de pliegues subcutáneos de una amplia población de atletas. Los pliegues tricipital, subescapular, suprailiaco, abdominal, muslo y pierna fueron medidos de acuerdo a la normativa ISAK (Sociedad Internacional para el Avance de la Cineantropometría) en 2500 atletas. La suma de los seis pliegues así como las sumas parciales de diferentes segmentos corporales (superior, troncal e inferior) fueron calculadas. Los datos muestran que los valores más altos de pliegues subcutáneos fueron observados en el muslo y zona abdominal, siendo el pliegue subescapular el menor. Las mujeres presentaron valores más altos de pliegues subcutáneos que los hombres. Respecto a las sumas parciales, los segmentos corporales troncal e inferior presentaron las mayores puntuaciones. Los practicantes de hockey, balonmano, fútbol sala, remo, submarinismo y tenis de mesa mostraron los mayores porcentajes de grasa. En conclusión, los cálculos de masa grasa en atletas deberían de considerar el sumatorio de los seis pliegues subcutáneos. Además, la grasa corporal tiende a estar localizada en los segmentos corporales troncal e inferior y varía según el género y la disciplina deportiva...