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1.
Sci Rep ; 14(1): 3795, 2024 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-38361036

RESUMO

This in vitro study evaluated the bacterial reduction provided by the EndoActivator (EA), Easy Clean (EC), passive ultrasonic irrigation (PUI), and XP-Endo Finisher. Eight-four mesial roots of mandibular first molars were instrumented, inoculated with Enterococcus faecalis, and divided into four groups (n. 20). Bacterial reduction in the main canals and dentinal tubules were respectively determined by MTT assays and Live/Dead BackLight technique through confocal laser scanning microscopy (CLSM) at 50, 100, and 150 µm in-depth (n. 10 per group). Statistical analyses were conducted following a significance level of 95% (P < 0.05). A significant statistical difference was just identified between XPF and EC in the main canals. In the dentinal tubules from the main root canals, at 100 and 150 µm in-depths, significant statistical differences were only observed between XPF and EC (P = 0.027) for the former and between XPF and EC (P = 0.011) and XPF and PUI (P = 0.021) for the latter. In the dentinal tubules from the isthmus, at 100 µm in-depth, statistically relevant differences did occur between XPF and EC (P = 0.038) and EC and EA (P = 0.029). At 150 µm in-depth, these differences were only significant by comparing XPF and PUI (P = 0.025) and XPF and EC (P = 0.036). Although no irrigation method could thoroughly disinfect the RCS, bacterial reduction indexes were generally better after using XPF.


Assuntos
Desinfecção , Preparo de Canal Radicular , Preparo de Canal Radicular/métodos , Desinfecção/métodos , Cavidade Pulpar , Irrigantes do Canal Radicular , Tratamento do Canal Radicular/métodos , Bactérias , Hipoclorito de Sódio
2.
J Bodyw Mov Ther ; 21(1): 109-116, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28167165

RESUMO

AIMS: To analyze and compare the electromyographic activity of the temporal (anterior portion) and masseter muscles among Karate-Do athletes before and after training, with and without the use of a mouthguard. METHODS: Twenty athletes (14 males and 6 females) with a mean age of 23.7 ± 7.5 years participated. They had surface electromyography recordings taken of their bilateral temporal and masseter muscles before and after training under the following conditions: no mouthguard, with a ready-made mouthguard, and with a custom-made mouthguard. Activity was examined at mandibular rest, while clenching, and at maximum voluntary contraction. The data were normalized using the mean maximum voluntary contraction. RESULTS: The right (p = 0.005) and left (p = 0.015) temporal muscles showed significantly lower electromyographic activity with a custom-made mouthguard compared with no mouthguard after training while clenching. The electromyographic activity of the temporal and masseter muscles did not show significant differences when tested at mandibular rest and while clenching before or after training with a custom-made mouthguard (p > 0.05). CONCLUSION: The use of a custom-made mouthguard preserved participants' electromyographic profiles before and after training; thus, they allow for stable muscle activity during the training of Karate-Do athletes.


Assuntos
Atletas , Artes Marciais/fisiologia , Músculos da Mastigação/fisiologia , Protetores Bucais , Contração Muscular/fisiologia , Adolescente , Adulto , Eletromiografia , Feminino , Humanos , Masculino , Músculo Masseter/fisiologia , Músculo Temporal/fisiologia , Adulto Jovem
3.
Dent Traumatol ; 32(4): 281-5, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26511663

RESUMO

BACKGROUND/AIM: Facial trauma in sports has been associated with temporomandibular disorders. Because of the intensity and duration of training needed for elite-level competitions, high-performance athletes can have two to five times more traumatic injuries than recreational athletes. This study aimed to investigate the prevalence of temporomandibular disorders in high-performance martial arts fighters and compare it with the prevalence in recreational athletes and non-athletes. MATERIAL AND METHODS: The Research Diagnostic Criteria for Temporomandibular Disorders was used to diagnose and classify professional karate-do practitioners (group I; n = 24), amateur karate-do practitioners (group II; n = 17), high-performance mixed martial arts fighters (group III; n = 13), and non-athletes (n = 28). The groups were compared with the chi-square test and tested for the difference between two proportions using a significance level of 5% (P < 0.05). RESULTS: The prevalence of temporomandibular disorders in groups I (54.2%; P = 0.003) and III (61.5%; P = 0.002) was significantly higher than in group IV (14.3%). The prevalence in group II was similar to that in group IV (P > 0.05). A diagnosis of arthralgia from disk displacement was made more frequently in groups I (45.8%; P = 0.013) and III (38.5%; P = 0.012) than in group IV (7.1%). The chronic pain associated with TMD was low intensity and low disability. CONCLUSIONS: While there was a high prevalence of temporomandibular disorders in the professional athletes in our study, the prevalence of the condition in recreational athletes was similar to that in individuals who did not practice martial arts.


Assuntos
Traumatismos Faciais , Artes Marciais , Transtornos da Articulação Temporomandibular/epidemiologia , Atletas , Humanos , Prevalência , Transtornos da Articulação Temporomandibular/etiologia
4.
Microb Pathog ; 51(6): 421-5, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21925586

RESUMO

The installation of metal devices leads to an increase in the salivary concentration of metal ions and in the growth of salivary Candida spp. However, the relationship between released metal ions and Candida virulence has not been previously examined. The objective of this study was to evaluate whether metal ions affect fungal virulence. We prepared culture media containing Ni(2+), Fe(3+), Cr(3+), Co(2+) or a mixture of these metal ions at concentrations similar to those released in saliva of orthodontic patients. Biofilms of Candida albicans SC5314 were grown for 72 h and their biomasses were determined. The supernatants were analyzed for secretory aspartyl protease (SAP) and hemolysin activities. To verify changes in virulence following treatment with metals, proteolytic and hemolytic activities were converted into specific activities. The results revealed that all ions, except Co(2+), caused increases in biofilm biomass. In addition, Ni(2+) caused an increase in SAP activity and Fe(3+) reduced hemolytic activity. However, the SAP and hemolysin activities in the presence of the mixture of ions did not differ from those of control. These results indicate that metal ions released during the degradation of orthodontic appliances can modulate virulence factors in C. albicans biofilms.


Assuntos
Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Candida albicans/efeitos dos fármacos , Candida albicans/fisiologia , Metais/metabolismo , Aparelhos Ortodônticos/microbiologia , Ácido Aspártico Proteases/metabolismo , Biomassa , Candida albicans/patogenicidade , Cátions/metabolismo , Meios de Cultura/química , Proteínas Hemolisinas/metabolismo , Humanos , Virulência
5.
Scand J Infect Dis ; 36(10): 758-61, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15513404

RESUMO

A case of non-invasive aspergillosis affecting the maxillary sinus is presented. Reference is made to its possible aetiology (spores introduced to the antrum at the time of a root canal overfilling with a zinc oxide paste). A review is presented and the management of the case is described.


Assuntos
Aspergilose/diagnóstico , Aspergillus fumigatus/isolamento & purificação , Sinusite Maxilar/diagnóstico , Tratamento do Canal Radicular/efeitos adversos , Adulto , Antifúngicos/uso terapêutico , Aspergilose/terapia , Terapia Combinada/métodos , Desbridamento/métodos , Feminino , Seguimentos , Humanos , Sinusite Maxilar/terapia , Medição de Risco , Tratamento do Canal Radicular/métodos , Índice de Gravidade de Doença , Resultado do Tratamento
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