RESUMO
Female rats were fed a normal or hypoproteic diet during the phases of gestation and lactation. The male offspring of these rats were grown to adulthood and used to study the effects of maternal protein malnutrition on progeny. The adult male rats were pretreated with either saline or LPS and subjected to behavioral tests 2 and 6 h after administration. Tumor necrosis factor (TNF-α), corticosterone and body temperature were the parameters used for assessment. Two hours after LPS administration, sickness behavior was developed in all the animals, regardless of maternal protein malnutrition. After 6 h of LPS administration, sickness behavior was more pronounced in the rats that had been subjected to maternal protein malnutrition. Only the rats with maternal protein malnutrition expressed an increase in the plasma levels of TNF-α and corticosterone. Maternal protein malnutrition prolongs sickness behaviors in offspring.
Assuntos
Comportamento de Doença , Complicações na Gravidez/fisiopatologia , Efeitos Tardios da Exposição Pré-Natal , Deficiência de Proteína/fisiopatologia , Animais , Corticosterona/sangue , Endotoxemia/sangue , Endotoxemia/psicologia , Feminino , Febre/etiologia , Lactação , Lipopolissacarídeos/toxicidade , Masculino , Gravidez , Ratos , Ratos Wistar , Comportamento Social , Natação , Fator de Necrose Tumoral alfa/sangueRESUMO
The objective of this study was to investigate the effect of exposure to maternal consumption of a hyperenergetic, highly palatable diet, known as the cafeteria diet, during the gestation period on the development and behavior of offspring. For this, we used pregnant female mice that were fed a normal or a cafeteria diet during the gestation period. The evaluation of maternal behavior in lactating dams was performed from the second to the eighth day postpartum (PND 2-8). Weight gain, feed intake, and energy intake were recorded during the gestation period. In the offspring, reflex parameters and physical development were evaluated during the lactation period and when they reached adolescence. Behavioral performance was evaluated in light-dark, open-field, and play behavior tests. In addition, biochemical parameters of the dams and the adolescent offspring were evaluated. The cafeteria diet during gestation altered maternal behavior and the onset of physical and neurodevelopmental landmarks and had an impact on emotional and play behavior in adolescent offspring. In conclusion, our results demonstrate that exposure to maternal consumption of a cafeteria diet during the gestation period can program developmental and behavioral courses in the offspring.
Assuntos
Dieta , Comportamento Exploratório/fisiologia , Idade Gestacional , Comportamento Materno/fisiologia , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Adaptação Fisiológica , Animais , Animais Recém-Nascidos , Peso Corporal/fisiologia , Ingestão de Energia/fisiologia , Comportamento Alimentar , Feminino , Masculino , Fenômenos Fisiológicos da Nutrição Materna , Camundongos , Jogos e Brinquedos , GravidezRESUMO
Dipyrone (metamizole), a powerful drug, is widely used as an analgesic and antipyretic; however, the safety of its use during lactation and the potential impact on the offspring are not well established. This study aimed to determine the effect of maternal dipyrone treatment during lactation on offspring development and emotional behavior and on the dam's maternal behavior. Hence, on postnatal day (PND) 2, drinking water only or drinking water containing dipyrone at doses of 100, 300, and 500mg/kg/day, were offered to lactating mothers up to PND9. Thereafter, all mice were provided regular drinking water. On PND2, all litters were culled to 8 pups (4 males and 4 females). Maternal behavior was evaluated at PND3, 6, 9, and 12, and at PND7 we evaluated locomotor activity in the open field. Reflex parameters and physical development of offspring were evaluated during lactation. At PND7, analysis of ultrasonic vocalization (USV) was performed. When the animals reached adolescence, we evaluated their performance in the open field, elevated plus maze (EPM), and marble burying. Our data demonstrated that maternal dipyrone treatment during lactation not only altered maternal behavior and the onset of physical and neurodevelopmental landmarks but also had an impact on anxiety-like behavior in offspring.
Assuntos
Anti-Inflamatórios não Esteroides/toxicidade , Ansiedade/induzido quimicamente , Dipirona/toxicidade , Comportamento Materno/efeitos dos fármacos , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/fisiopatologia , Fatores Etários , Animais , Animais Recém-Nascidos , Deficiências do Desenvolvimento/etiologia , Relação Dose-Resposta a Droga , Comportamento de Ingestão de Líquido/efeitos dos fármacos , Comportamento Exploratório/efeitos dos fármacos , Feminino , Humanos , Lactação/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Gravidez , Vocalização Animal/efeitos dos fármacosRESUMO
OBJECTIVE: The aim of this study was to evaluate the effects of protein malnutrition during pregnancy on maternal behavior, on the early behavior in pups by ultrasonic vocalizations (USVs) emission, and on the behavior of offspring in adulthood in an elevated T-maze. METHODS: Pregnant female rats were fed a normal protein-powdered diet (22% casein; control) or a low-protein (hypoproteic) diet (6% casein; protein restriction) during the first 2 weeks of pregnancy. On the fifth postpartum day (PND5), the number of USV was rated. On PND7, maternal behavior was assessed. Male offspring in adulthood were evaluated for behavioral performance in an elevated T-maze. RESULTS: Our results demonstrated that a hypoproteic diet during early pregnancy increased the maternal behavior, increased the number of USV by pups, and reduced the inhibitory avoidance responses in an elevated T-maze during adulthood. In addition, there was a reduction in weight gain of rats during pregnancy and of offspring during lactation. CONCLUSION: In conclusion, the data found in our study suggest that the increase in USV emitted by pups due to hypoproteic diet during pregnancy accentuated maternal behavior. In addition, an increase in maternal care promoted the reduction in anxiety-like behavior in adult male offspring.