RESUMO
Polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) which relies on the amplification of a 439-bp portion of the hsp65 gene present in all mycobacteria, followed by two distinct digestions (with BstEII and HaeIII) of the PCR product, offers a rapid and easy alternative that allows identification of the species without the need for specialized equipment. Wild leprosy in the nine-banded armadillo (Dasypus novemcinctus) is characterized by the presence of multiple bacilli in internal organs such as lymph nodes, spleen and liver, as well as in nerves and skin. We could observe this in 9 out of 132 animals captured in Corrientes, Argentina, an area endemic for leprosy in humans. Mycobacterium leprae were recognized in those naturally infected animals through different techniques. Three samples of extracted DNA of the mycobacteria present in the spleen, liver and popliteal lymph node of a naturally infected animal during the Experimental Program in Armadillo (PEA) and three samples of human lepromas were processed by PRA. The patterns of the six samples analyzed were identical and were characteristic of M. leprae. These studies, made for the first time in Argentina, corroborate the initial discoveries in South America made by our investigative group on the detection of armadillos naturally infected with the Hansen bacillus.
Assuntos
Animais , Mycobacterium leprae/fisiologia , Mycobacterium leprae/genética , Mycobacterium leprae/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodosRESUMO
Los orígenes de la arteria intercostal superior apartir de la arteria axilar o bien de la arteria cervucal profunda (ACP) descriras por CHAVEAU & ARLONG (1905 por ciento) como anomalías, no fueron observados en nuestra experiencia. El desplazamiento de la arteria escapular dorsal hacia el 3º espacio intercostal y de la arteria cervical profunda hacia el 2º espacio intercostal descritos por este autor, fueron observados en los casos examinados. A su vez, el origen en común de la arteria cervical superficial y arteria torácica externa no fue observado. Si bien la ATE puede originarse de la arteria torácica interna como describe CHAVEAU & ARLOING, en nuestras diseccionesfue observado como colateral de la arteria torácica interna. El comportamiento de la arteria costocervical y arteria cervical profunda cuanto a su origen, a partir de un tronco común, Coincide con las variaciones descritas por GETTY, (1982). Las aseveraciones efectuadas por BOSSI et al., (1903) sobre el mayor porcentaje de variaciones en la arteria costocervical y arteria torácica externa, no coinciden con nuestras descripciones. Existe coincidencia con BOSSI et al. En cuanto al uso del término <
Assuntos
Animais , Equidae/anatomia & histologia , Tronco Braquiocefálico/anatomia & histologia , Artéria Axilar/anatomia & histologia , Artéria Torácica Interna/anatomia & histologia , Tronco Braquiocefálico/anormalidades , Artéria Vertebral/anatomia & histologiaRESUMO
A case of caecum nematodiasis is described in a guinea fowl (Numida melagris) from the Municipal Zoo, Presidencia Roque Saenz Peña (Chaco) Argentina. Nematodes obtained from the caecum were observed in optic microscopy. According to their morphometric characteristics and location in the definitive host, were identified as belonging to the family heterakidae, species Heterakis gallinarum, (Schrank, 1788) Maden, 1949
Assuntos
Animais , Nematoides/isolamento & purificação , Aves Domésticas/parasitologia , Argentina , Vetores de Doenças , Intestinos/parasitologia , Nematoides/anatomia & histologia , Nematoides/patogenicidade , Infecções por NematoidesRESUMO
A case of gastric nematodiasis is describes in a gineafowl (Numida meleagris) from the Municipal Zoo, Presidencia Roque Saenz Peña (Chaco) Argentina, nematodes obtained from the glandular stomach were observed in optic microscopy. According to their morphometric characteristies and location in the definitivo host, were identified as belonging to the family Acuariidae, subfamily Acuariinae, species Dispharynx nasuta Rudolphi, 1819