RESUMO
MicroRNAs are heterochronic molecules important during brain development, which could be altered by gestational diabetes mellitus (GDM). To explore these molecules in maternal serum, we performed an RT-qPCR analysis. Our results revealed the heterochronic character of some neural development-related microRNA in serum samples of pregnant women. In relation to the first trimester, higher levels of miR-183-5p, -200b-3p, and -125-5p in the second trimester, and higher levels of miR-137 in the third trimester, were found. Furthermore, an insult such as GDM led to higher levels of miR-183-5p, -200b-3p, -125-5p, and -1290 relative to the control in the first trimester, which might be related to changes in neurogenesis and cell proliferation. An in silico analysis suggested that increased microRNAs in the second trimester in the control contributed to cell proliferation and neuron differentiation and that the rise in miR-137 in the third trimester led to neuron maturation. In the diabetic, higher levels of the microRNAs in the first trimester suggested alterations in cell proliferation and neuron differentiation. In conclusion, we showed that fetal-related microRNAs can be detected in the serum of pregnant woman and exhibit temporary regulation during pregnancy and that microRNAs involved in cell proliferation and neuron differentiation are upregulated under GDM.
Assuntos
Sistema Nervoso Central/crescimento & desenvolvimento , Sistema Nervoso Central/metabolismo , Diabetes Gestacional/sangue , MicroRNAs/sangue , Adolescente , Adulto , Simulação por Computador , Feminino , Humanos , Gravidez , Primeiro Trimestre da Gravidez , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Adulto JovemRESUMO
In total, 189 consecutive women diagnosed with SLE were evaluated using the ACR 1990 criteria for fibromyalgia. Patients were classified into three subgroups. The fibromyalgia group (FM) included patients experiencing pain on palpation in at least 11 of the 18 tender points examined, as well as having a history of widespread pain for at least three months. Patients who were noted to have pain in fewer than four quadrants with less than 11 of 18 tender points were considered to have regional pain (RP). All patients who did not meet criteria for either FM or RP were classified as having no pain (NP). Measurement of SLE disease activity, sleep complaints, depression, fatigue severity and health status were performed. Only 18 of the SLE patients (9.5%) (95% CI 5.3-14%) fulfilled the ACR criteria for the classification of FM. Of the patients, 106 (56.1%) fulfilled criteria for RP and had a number of tender points of 5.4 +/- 3.4, and the rest of the patients (34.4%) had no tenderness at specific tender point sites. Age, body mass index, educational level and disease duration were comparable between the groups. FM and RP groups had different patterns of symptoms prevalence, with dysmenorrhea being more distinctive for FM. Sleep disturbances were more severe in the FM than in the RP group. Daytime complaints such as sleepiness, fatigue and depression were similar for RP and FM groups, but patients with FM reported more disability. Fibromyalgia is not common in Mexican patients with SLE and has a different pattern of symptoms in RP and NP patients. These data add evidence that ethnicity can play an important role in FM manifestations.
Assuntos
Fibromialgia/etnologia , Lúpus Eritematoso Sistêmico/etnologia , Adolescente , Adulto , Idoso , Índice de Massa Corporal , Avaliação da Deficiência , Feminino , Fibromialgia/classificação , Fibromialgia/complicações , Humanos , Lúpus Eritematoso Sistêmico/complicações , México/epidemiologia , Pessoa de Meia-Idade , Medição da Dor , Prevalência , Índice de Gravidade de Doença , Transtornos do Sono-Vigília/etiologiaRESUMO
We report two children with severe neurological complications after having been stung by a scorpion. Clinical and MRI findings suggested brain infarcts. The lesions seen were in pons in one child and the right hemisphere in the other. The latter also showed possible hyperemia in the infarcted area. No vascular occlusions were observed and we therefore think the brain infarcts were a consequence of the scorpion sting. The cause of the infarct may be hypotension, shock or depressed left ventricular function, all of which are frequent in severe poisoning by scorpion sting.
Assuntos
Infarto Encefálico/etiologia , Picadas de Escorpião/complicações , Escorpiões , Animais , Antivenenos/uso terapêutico , Edema Encefálico/etiologia , Criança , Feminino , Humanos , Hiperemia/etiologia , Angiografia por Ressonância Magnética , Imageamento por Ressonância Magnética , Masculino , Ponte/irrigação sanguínea , Venenos de Escorpião/efeitos adversos , Venenos de Escorpião/antagonistas & inibidores , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVE: To assess objective and subjective evidence of sleep disorders in patients with systemic lupus erythematosus (SLE) and to examine correlations between parameters of lupus activity, depression, and sleep disturbances. METHODS: Fourteen SLE patients and 11 normal control subjects of similar age underwent all-night polysomnography on 3 consecutive nights. The patients and controls were also evaluated for daytime sleepiness by the Multiple Sleep Latency Test and completed a sleep disorders questionnaire and the Beck Depression Inventory. RESULTS: The polysomnographic data showed that sleep in SLE patients was characterized by respiratory and movement disorders. These intrinsic primary sleep disorders are related to the symptom of restless, poor sleep at night. Lupus patients were more sleepy during the day, and their sleepiness was related to sleep fragmentation, with more arousals and stage transitions than the control group. Disease activity was associated with decreases in sleep efficiency and delta sleep and with increases in sleep fragmentation. Depression was not correlated with the activity of the disease. CONCLUSION: There is an enhanced presence of sleep disorders in patients with SLE. The most frequent primary sleep disorders are respiratory and movement disorders.
Assuntos
Lúpus Eritematoso Sistêmico/complicações , Transtornos do Sono-Vigília/etiologia , Transtornos do Sono-Vigília/psicologia , Adulto , Depressão/complicações , Feminino , Humanos , Lúpus Eritematoso Sistêmico/fisiopatologia , Pessoa de Meia-Idade , Pacientes AmbulatoriaisRESUMO
Evidence in support for the concept of the so-called 'siesta culture' is not well developed and has, to date, relied largely on qualitative anthropological data. Presumably such cultures are characterized by a strong tendency for daytime naps and daytime sleepiness, phenomena which may partially represent the effects of geographic, climatic or light conditions and/or cultural influences. In this study we surveyed the nocturnal sleep habits and daytime sleep tendencies of 577 Mexican college students residing in Mexico City (19 degrees N latitude). Results indicated a number of parallels between the reported sleep habits of these students and those reported from other cultures at latitudes far to the north (North America, Europe), such as longer sleep at the weekends, an association between snoring and daytime sleepiness and a lack of relationship between nocturnal sleep duration and the reported tendency to nap. There was some suggestion that these Mexican students may actually nap less when compared to other college student populations. Taken together, these results call into question what is meant by the concept of a 'siesta culture', at least in this urban, educated, upper social economic scale (SES) population, and suggest that future studies in equatorial regions be undertaken to further appreciate the role of climate, photoperiod and/or culture in the tendency for humans to nap during the day.
Assuntos
Cultura , Sono/fisiologia , Estudantes/psicologia , População Urbana , Adolescente , Adulto , Fatores Etários , Estudos de Coortes , Distúrbios do Sono por Sonolência Excessiva/diagnóstico , Feminino , Humanos , Masculino , México , Sono/efeitos dos fármacos , Ronco/diagnóstico , Inquéritos e Questionários , Fatores de Tempo , VigíliaRESUMO
The purpose of this study was to describe the factor structure of the Sleep-Wake Activity Inventory (SWAI) in a Mexican population. In a sample of 722 Mexican college students, we replicated five of the six factors originally described in the SWAI. Retained factors included: excessive daytime sleepiness (similarity coefficient of 0.735), psychic distress (0.609), social desirability (0.638), individual's ability to relax (0.864), and nocturnal sleep (0.660). These results confirm the factor structure and extend the possible utility of the SWAI in a siesta culture.