RESUMO
Recent studies found folic acid is associated with lower blood lead (Pb) levels, and folate deficient children are more susceptible to the negative cognitive effects of Pb. This study evaluated the protective effects of folate supplementation against Pb exposure in rat pups and the mechanisms of protection. A total of 72 rats were used. Thirty were administered Pb only; 30, Pb and folic acid at the same time; and 12, only physiological saline. Protective effects of folic acid were examined at 14, 21, and 28 days after treatment. Lower blood Pb levels were found in all of the samples collected from the rats treated with folic acid. Downregulation of Bc1-2 expression and upregulation of Bax expression were observed in the neurons of folic acid-treated rats. Significantly more hematoxylin and eosin stained neurons were found in the folic acid treatment group. Nuclear enrichment and neuron apoptosis were observed by electron microscopy in the Pb-treated group. In conclusion, this study demonstrated that folic acid supplementation might offer efficient protective effects against Pb poisoning in rat pups, which was associated with less neuron damage and lower blood levels of Pb.
Assuntos
Sistema Nervoso Central/efeitos dos fármacos , Ácido Fólico/uso terapêutico , Chumbo/toxicidade , Animais , Poluentes Ambientais/toxicidade , Síndromes Neurotóxicas/tratamento farmacológico , Síndromes Neurotóxicas/prevenção & controle , Ratos , Ratos Sprague-DawleyRESUMO
The gene for the nucleocapsid (N) protein of canine distemper virus was cloned into the pMD-18T vector, and positive recombinant plasmids were obtained by enzyme digestion and sequencing. After digestion by both EcoRI and KpnI, the plasmid was directionally cloned into the eukaryotic expression vector pcDNA; the positive clone pcDNA-N was screened by electrophoresis and then transfected into COS-7 cells. Immunofluorescence analysis results showed that the canine distemper virus N protein was expressed in the cytoplasm of transfected COS-7 cells. After emulsification in Freund's adjuvant, the recombinant plasmid pcDNA-N was injected into the abdominal cavity of 8-week-old BABL/c mice, with the pcDNA original vector used as a negative control. Mice were immunized 3 times every 2 weeks. The blood of immunized mice was drawn 2 weeks after completing the immunizations to measure titer levels. The antibody titer in the pcDNA-N test was 10(1.62 ± 0.164), while in the control group this value was 10(0.52 ± 0.56), indicating that specific humoral immunity was induced in canine distemper virus nucleocapsid protein-immunized mice.
Assuntos
Vírus da Cinomose Canina/genética , Vírus da Cinomose Canina/imunologia , Animais , Anticorpos Antivirais/sangue , Clonagem Molecular , Cães , Feminino , Imunização/veterinária , Camundongos , Camundongos Endogâmicos BALB C , Proteínas do Nucleocapsídeo/genética , Vacinas Virais/imunologiaRESUMO
We optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in China. We selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 Sumatran tiger microsatellite loci using agarose gel electrophoresis. Next, the set of optimized genetic markers was used to analyze the genetic variation in an outbred population of orange tabby cats in China by simple-tandem repeat scanning. Thirty-one loci rich in polymorphisms were selected and the highest allele number in a single locus was 8. Analysis of the orange tabby cat population illustrated that the average observed number of alleles, mean effective allele number, mean Shannon's information index, mean expected heterozygosity, and observed heterozygosity were 3.8387, 2.4027, 0.9787, 0.5565, and 0.5528, respectively. The 31 microsatellite markers used were polymorphic and suitable for analyzing the genetic structure of cats. The population of orange tabby cats was confirmed to be a well-outbred stock.
Assuntos
Gatos/genética , Loci Gênicos , Genética Populacional , Repetições de Microssatélites , Alelos , Animais , China , Marcadores Genéticos , Heterozigoto , Filogeografia , Polimorfismo Genético , Análise de Sequência de DNARESUMO
This study aimed to prepare monoclonal antibody of feline calicivirus (FCV) and identify its basic biological characteristics. Saturated ammonium sulfate precipitation, combined differential centrifugation, and cesium chloride density gradient centrifugation were used for the purification of FCV. The purified FCV was used as antigen to immunize BALB/c mice. The hybridoma lines of anti-FCV monoclonal antibodies were established using cell fusion and hybridoma screening techniques. The subtypes of the monoclonal antibody were identified. The results showed that 3 strains of hybridoma cell lines stably secreted anti-FCV monoclonal antibody; they were named as D8, E5, and H4. The D8 and E5 were IgM subtype antibodies, and H4 was IgG2b subtype antibody. The monoclonal antibody obtained shared no cross-reactivity with feline parvovirus, canine parvovirus, and canine distemper virus. According to the different recognition sites of 2 monoclonal antibodies E5 and H4 to the FCV, they were used to coat microtiter plates and prepare 2 enzyme-labeled secondary antibodies to establish double-antibody sandwich enzyme-linked immunosorbent assay detecting method.