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Fundamentally precision oncology illustrates the path in which molecular profiling of tumors can illuminate their biological behavior, diversity, and likely outcomes by identifying distinct genetic mutations, protein levels, and other biomarkers that underpin cancer progression. Next-generation sequencing became an indispensable diagnostic tool for diagnosis and treatment guidance in current clinical practice. Nowadays, tissue analysis benefits from further support through methods like comprehensive genomic profiling and liquid biopsies. However, precision medicine in the field of oncology presents specific hurdles, such as the cost-benefit balance and widespread accessibility, particularly in countries with low- and middle-income. A key issue is how to effectively extend next-generation sequencing to all cancer patients, thus empowering treatment decision-making. Concerns also extend to the quality and preservation of tissue samples, as well as the evaluation of health technologies. Moreover, as technology advances, novel next-generation sequencing assessments are being developed, including the study of Fragmentomics. Therefore, our objective was to delineate the primary uses of next-generation sequencing, discussing its' applications, limitations, and prospective paths forward in Oncology.

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Resumo O objetivo foi analisar e aprimorar a resposta da Atenção Primária à Saúde (APS) ao cuidado dos casos de violência doméstica contra a mulher (VDCM), desenvolvendo, implementando e avaliando uma intervenção. Pesquisa piloto de avaliação do antes e depois da implementação de uma intervenção, utilizando métodos mistos e realizada em três fases - linha de base, intervenção e avaliação - entre agosto/2017 e março/2019 em duas Unidades Básicas de Saúde (UBS) do Município de São Paulo. Apresentamos neste artigo o detalhamento e a avaliação da intervenção, realizada 6-12 meses após implementação. A intervenção foi desenvolvida com base nos achados da primeira fase e em consonância com a política de saúde que estabelece os Núcleos de Prevenção à Violência (NPV), consistindo em: elaboração de fluxo assistencial; capacitação geral para todos os trabalhadores e específica para o NPV; elaboração de material educativo e discussões mensais de caso durante 6 meses. A avaliação mostrou aceitabilidade entre os trabalhadores, aumentou a identificação e repertório para o cuidado dos casos de VDCM, fortalecendo o encaminhamento interno e à rede intersetorial. Identificamos obstáculos para plena implementação e sustentabilidade da intervenção.

Abstract The aim was to analyse and improve the Primary Health Care (PHC) response to domestic violence against women (DVAW) by developing, implementing and evaluating an intervention. A pilot study evaluating the before and after of intervention implementation, using mixed methods and carried out in three phases - formative, intervention and evaluation - between August 2017 and March 2019 in two Basic Health Units (UBS) in the city of São Paulo. In this paper, we present the details and evaluation of the intervention, carried out six to twelve months after its implementation. The intervention was developed based on the findings of the formative phase and in line with the health policy that establishes the Violence Prevention Nucleus (NPV) and consisted of stablishing a care pathway; general training for all workers and specific training for the NPV; drawing up educational material and monthly case discussions over 6 months. The evaluation showed acceptability among the workers, increased identification and repertoire for caring for cases of DVAW, strengthening internal referral and the intersectoral network. We identified obstacles to the full implementation and sustainability of the intervention.

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NTRK1, 2, and 3 fusions are important therapeutic targets for NSCLC patients, but their prevalence in South American admixed populations needs to be better explored. NTRK fusion detection in small biopsies is a challenge, and distinct methodologies are used, such as RNA-based next-generation sequencing (NGS), immunohistochemistry, and RNA-based nCounter. This study aimed to evaluate the frequency and concordance of positive samples for NTRK fusions using a custom nCounter assay in a real-world scenario of a single institution in Brazil. Out of 147 NSCLC patients, 12 (8.2%) cases depicted pan-NTRK positivity by IHC. Due to the absence of biological material, RNA-based NGS and/or nCounter could be performed in six of the 12 IHC-positive cases (50%). We found one case exhibiting an NTRK1 fusion and another an NTRK3 gene fusion by both RNA-based NGS and nCounter techniques. Both NTRK fusions were detected in patients diagnosed with lung adenocarcinoma, with no history of tobacco consumption. Moreover, no concomitant EGFR, KRAS, and ALK gene alterations were detected in NTRK-positive patients. The concordance rate between IHC and RNA-based NGS was 33.4%, and between immunohistochemistry and nCounter was 40%. Our findings indicate that NTRK fusions in Brazilian NSCLC patients are relatively rare (1.3%), and RNA-based nCounter methodology is a suitable approach for NRTK fusion identification in small biopsies.

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Abstract Background Kidney disease is a rare manifestation of ankylosing spondylitis (AS) and its pathological alterations remain poorly described. The aim of this study was to investigate the clinical presentation and pathological alterations on kidney biopsy of AS patients and review and discuss the current literature on the issue. Methods: We retrospectively studied the clinical presentation and kidney pathological alterations of 15 Caucasian AS patients submitted to kidney biopsy between October 1985 and March 2021. Results: Patients were predominantly male (66.7%) with median age at the time of kideney biopsy of 47 years [IQR 34 - 62]. Median serum creatinine at presentation was 1.3 mg/dL [IQR 0.9 - 3] and most patients also had either proteinuria (85.7%) and/or hematuria (42.8%). The most common indication for kidney biopsy was nephrotic syndrome (33.3%), followed by acute or rapidly progressive kidney injury (20%) and chronic kidney disease of unknown etiology (20%). Chronic interstitial nephritis (CIN) (n=3) and AA amyloidosis (n=3) were the most common diagnosis. Others included IgA nephropathy (IgAN) (n=2), focal segmental glomerulosclerosis (n=2), membranous nephropathy (n=1), and immune complex-mediated membranoproliferative glomerulonephritis (IC-MPGN)(n=1). Conclusions: We present one of the largest series of biopsy-proven kidney disease in Caucasian AS patients. We found a lower prevalence of IgAN than previously reported in Asian cohorts. We found a higher prevalence of CIN and a lower prevalence of AA amyloidosis than that described in previous series of Caucasian patients. We also present the first case of AS-associated IC-MPGN.

Resumo Antecedentes: A doença renal é uma manifestação rara de espondilite anquilosante (EA) e as suas alterações patológicas permanecem pouco descritas. O objetivo deste estudo foi investigar a apresentação clínica e alterações patológicas na biópsia renal de doentes com EA bem como rever e discutir a literatura atual sobre o assunto. Métodos: Estudamos retrospectivamente a apresentação clínica e alterações patológicas renais de 15 doentes caucasianos com EA submetidos a biópsia renal entre Outubro de 1985 e Março de 2021. Resultados: Os doentes eram predominantemente homens (66,7%) com idade mediana no momento da biópsia de 47 anos [IIQ 34 - 62]. A creatinina sérica mediana na apresentação foi de 1,3 mg/dL [IIQ 0,9 - 3] e a maioria dos pacientes apresentava também proteinúria (85,7%) e/ou hematúria (42,8%). A indicação mais comum para biópsia renal foi a síndrome nefrótica (33,3%), seguida de lesão renal aguda ou rapidamente progressiva (20%) e doença renal crónica de etiologia desconhecida (20%). A Nefrite intersticial crónica (NIC) (n=3) e a amiloidose AA (n=3) foram os diagnósticos mais comuns. Outros incluíram nefropatia por IgA (NIgA) (n=2), glomeruloesclerose segmentar focal (n=2), nefropatia membranosa (n=1) e glomerulonefrite membranoproliferativa mediada por imunocomplexos (GNMP-IC) (n=1). Conclusões: Apresentamos uma das maiores séries de doenças renais comprovadas por biópsia em doentes caucasianos com EA. Encontramos uma prevalência de NIgA menor do que a relatada anteriormente em coortes asiáticas. Encontramos uma maior prevalência de NIC e uma prevalência menor de amiloidose AA do que a descrita em séries anteriores de pacientes caucasianos. Também apresentamos o primeiro caso de GNMP-IC associada à EA.

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BACKGROUND: Kidney disease is a rare manifestation of ankylosing spondylitis (AS) and its pathological alterations remain poorly described. The aim of this study was to investigate the clinical presentation and pathological alterations on kidney biopsy of AS patients and review and discuss the current literature on the issue. METHODS: We retrospectively studied the clinical presentation and kidney pathological alterations of 15 Caucasian AS patients submitted to kidney biopsy between October 1985 and March 2021. RESULTS: Patients were predominantly male (66.7%) with median age at the time of kideney biopsy of 47 years [IQR 34 - 62]. Median serum creatinine at presentation was 1.3 mg/dL [IQR 0.9 - 3] and most patients also had either proteinuria (85.7%) and/or hematuria (42.8%). The most common indication for kidney biopsy was nephrotic syndrome (33.3%), followed by acute or rapidly progressive kidney injury (20%) and chronic kidney disease of unknown etiology (20%). Chronic interstitial nephritis (CIN) (n=3) and AA amyloidosis (n=3) were the most common diagnosis. Others included IgA nephropathy (IgAN) (n=2), focal segmental glomerulosclerosis (n=2), membranous nephropathy (n=1), and immune complex-mediated membranoproliferative glomerulonephritis (IC-MPGN)(n=1). CONCLUSIONS: We present one of the largest series of biopsy-proven kidney disease in Caucasian AS patients. We found a lower prevalence of IgAN than previously reported in Asian cohorts. We found a higher prevalence of CIN and a lower prevalence of AA amyloidosis than that described in previous series of Caucasian patients. We also present the first case of AS-associated IC-MPGN.

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É difícil reconhecer o sexo forçado vivido nas relações sexuais no âmbito doméstico como violência. Há também uma imprecisão entre a violência, tal como no sexo forçado, e a desigualdade de gênero, como na aceitação do dever marital. Buscou-se compreender o que profissionais da Atenção Primária pensam sobre essas duas experiências, como interpretam relatos das mulheres e o que fazem sobre isso. Entrevistados, os profissionais dizem que sexo forçado ou sexo sem consentimento explícito são ambos violência, e assim devem ser nomeados. Agindo desse modo, eles pensam esclarecer suas pacientes acerca dos direitos das mulheres. No entanto, no dia a dia, nem todos o fazem e ninguém reconheceu ou nomeou a aceitação do dever marital como desigualdade de gênero. Conclui-se que, se a violência está presente como questão, sua distinção quanto à desigualdade de gênero ainda é um desafio.(AU)

Es difícil reconocer el sexo forzado vivido en las relaciones sexuales en el ámbito doméstico como violencia. Hay también una imprecisión entre la violencia, tal como en el sexo forzado, y la desigualdad de género, como en la aceptación del deber conyugal. Se buscó entender lo que los profesionales de la atención primaria piensan sobre esas dos experiencias, cómo interpretan los relatos de las mujeres y qué hacen sobre eso. Al ser entrevistados, los profesionales decían que el sexo forzado o el sexo sin consentimiento explícito son violencia y deben ser denominados como tal. Actuando así, ellos piensan aclarar a sus pacientes los derechos de las mujeres. Sin embargo, en el cotidiano no todos lo hacen y ninguno reconoció o nombró la aceptación del deber conyugal como desigualdad de género. Se concluyó que la violencia está presente como cuestión y que su distinción con relación a la igualdad de género todavía es un desafío.(AU)

Studies show how difficult it is to recognize what is experienced in sexual relationships within households. There is an inaccuracy between violence as in the forced sex, and gender inequality as in the acceptance of the marital duty. We aimed to understand what health care providers think about these two experiences, how they interpret women's reports and what they do about it. Interviewed, the professionals say that both forced sex and sex with no explicit consent are violence and so they should be named. By doing so, professionals intend to enlighten their patients about women's rights. However, in everyday life not everyone does and no one recognized or named marital duty as gender inequality. We conclude that if violence is present as an issue, its distinction in relation to gender inequality is still a challenge.(AU)

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O objetivo do estudo foi verificar a prevalência do transtorno de ansiedade e fatores sociodemográficos, comportamentais e aspectos de saúde associados em crianças de três a 10 anos de idade, durante a pandemia da COVID-19. Estudo de delineamento transversal, realizado na região do Triângulo Mineiro, estado de Minas Gerais, Brasil. Utilizou-se a Escala Spence de Ansiedade Infantil, versão para os pais, para avaliar os sintomas das principais perturbações de ansiedade em crianças. Para comparar proporções utilizou-se o teste Qui-quadrado, os fatores associados ao transtorno de ansiedade foram encontrados por Regressão de Poisson, com intervalo de confiança de 95%. Participaram n=778 pais de crianças de três a 10 anos de idade. A prevalência do transtorno de ansiedade nas crianças foi de 1,7%. Crianças de pais analfabetos e com ensino fundamental II incompleto apresentaram razão de prevalência (RP) = 4,24 [(IC95%: 1,03 ­ 17,46), p=0,045] maior de transtorno de ansiedade que às crianças de pais com nível superior completo a pós-graduação, enquanto as crianças de pais com ensino fundamental a superior incompleto apresentaram uma RP = 2,64 [(IC95%: 0,72 ­ 9,72), p=0,045] em comparação às crianças de pais com nível superior completo a pós- graduação. Crianças com alterações nos padrões alimentares apresentaram uma RP = 11,10 [(IC95%: 1,59 ­ 77,42), p = 0,015] maior de transtorno de ansiedade, em relação às crianças que não possuíam alterações nos padrões alimentares. Concluiu-se que as variáveis que associaram ao transtorno de ansiedade foram o nível de escolaridade dos pais e alterações dos padrões alimentares das crianças.

The objective of the study was to verify the prevalence of anxiety disorder and sociodemographic, behavioral factors and associated health aspects in 3-10-year-old children, during the COVID-19 pandemic. Cross-sectional study carried out in the Triângulo Mineiro, region of Minas Gerais state, Brazil. The Spence Child Anxiety Scale, version for parents, was used to assess the symptoms of the anxiety disorders in children. The Chi-square test was used to compare proportions, the factors associated with the anxiety disorder were found by Regression Poisson, with a 95% confidence interval. Participated in this study 778 parents of 3-10-year-old children. The prevalence of anxiety disorder in children was 1.7%. Children of illiterate parents and with incomplete primary education had a prevalence ratio (PR) = 4.24 [(IC95%:1.03 ­ 17.46), p=0.045] higher for anxiety disorder than children of parents with complete higher education to graduate school, while children of parents with incomplete primary or higher education had a PR = 2.64 [(IC95%:0.72 ­ 9.72), p=0.045] compared to children of parents with a complete higher education at postgraduate level. Children with changes in eating patterns had a PR = 11.10 [(IC95%:1.59 ­ 77.42), p = 0.015] higher for anxiety disorder, compared to children who did not have changes in eating patterns. The study concluded that the variables associated with anxiety disorder were parents' education level and changes in children's eating patterns.

El objetivo del estudio fue verificar la prevalencia del trastorno de ansiedad y los factores sociodemográficos, conductuales y de salud asociados en niños de 3 a 10 años, durante la pandemia de COVID-19. Estudio transversal realizado en el Triângulo Mineiro, región del estado de Minas Gerais, Brasil. Se utilizó la Escala de Ansiedad Infantil de Spence, versión para padres, para evaluar los síntomas de los trastornos de ansiedad en los niños. Para la comparación de proporciones se utilizó la prueba de Chi- cuadrado, los factores asociados al trastorno de ansiedad fueron encontrados por Regresión de Poisson, con un intervalo de confianza del 95%. Participaron en este estudio 778 padres de niños de 3 a 10 años. La prevalencia del trastorno de ansiedad en niños fue del 1,7%. Los hijos de padres analfabetos y con educación primaria incompleta tuvieron una razón de prevalencia (RP) = 4,24 [(IC95%:1,03 ­ 17,46), p=0,045] mayor para el trastorno de ansiedad que los hijos de padres con educación superior completa a posgrado, mientras que los niños de padres con estudios primarios o superiores incompletos tuvo un PR = 2,64 [(IC95%:0,72 ­ 9,72), p=0,045] en comparación con los hijos de padres con estudios superiores completos a nivel de posgrado. Los niños con cambios en los patrones alimentarios tuvieron un PR = 11,10 [(IC95%:1,59 ­ 77,42), p = 0,015] mayor para el trastorno de ansiedad, en comparación con los niños que no tuvieron cambios en los patrones alimentarios. El estudio concluyó que las variables asociadas con el trastorno de ansiedad fueron el nivel educativo de los padres y los cambios en los patrones alimentarios de los niños.

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Resumo O trabalho em rede tem papel central na assistência a mulheres em situação de violência. Este estudo analisa as diferentes perspectivas desse trabalho para profissionais da Atenção Primária e profissionais de serviços especializados nas áreas de assistência social, assistência jurídica e segurança pública, na cidade de São Paulo, Brasil. Realizaram-se entrevistas semi-estruturadas com 16 profissionais dos serviços especializados e 46 da saúde. Os eixos para a análise temática foram: o que os profissionais sabem e pensam sobre os demais serviços; sua atuação a partir disso; e suas expectativas. Os dados revelaram conhecimento insuficiente sobre os distintos serviços, resultando em dificuldades comunicativas, bem como em encaminhamentos equivocados pautados em idealizações sobre como deveria atuar o outro serviço. Concluímos que cada setor é bastante autônomo e seus serviços partem de seu próprio campo de atuação para definir aquilo que seria melhor para a mulher. O conjunto funciona mais como uma trama de serviços do que como uma rede.

Abstract Networking plays a central role in assisting women in situations of violence. This study analyzes how different the work perspectives are for Primary Care professionals and specialized services professionals in the areas of social and law assistance, and public security in the city of São Paulo, Brazil. Semi-structured interviews were carried out with 16 professionals from specialized services and 46 from the health sector. The axes for a thematic analysis were: what professionals know and think about services other than their own; their performance based on that; and their expectations. The findings revealed insufficient knowledge of the different services, resulting in communication difficulties as well as wrong referrals to other services, based on how other services would ideally work. We concluded that each sector is autonomous and its services start from its own field of action to define what would be best for women. The set works more like a mesh of services than a network.

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Abstract Encapsulating peritoneal sclerosis is an uncommon but serious complication of peritoneal dialysis. In most cases, the symptoms appear after peritoneal dialysis withdrawal, which hampers its diagnosis. We present the case of a 44-years-old Caucasian male who had been on peritoneal dialysis for 6 years and 3 months and was switched to hemodialysis due to ultrafiltration failure. During his last months on peritoneal dialysis, he developed anorexia and asthenia, which were initially attributed to dialysis inadequacy. After hemodialysis induction, the patient developed abdominal pain, increased abdominal volume, obstipation alternating with diarrhea, and weight loss. Computed tomography showed de novo ascites. A diagnosis of early encapsulating peritoneal sclerosis was considered, and treatment was promptly initiated with nutritional support, oral prednisolone, and tamoxifen for one year. The patient progressed with resolution of the symptoms. One month after the end of the treatment, he underwent a successful kidney transplant and remain without any major intercurrences. A high level of clinical suspicion is crucial for the early diagnosis of encapsulating peritoneal sclerosis as the disease can be fatal in advanced stages. This case highlights that with early treatment, kidney transplantation can be successfully performed after an episode of encapsulating peritoneal sclerosis.

Resumo A esclerose peritoneal encapsulante é uma complicação incomum, mas grave, da diálise peritoneal. Na maioria dos casos, os sintomas aparecem após a suspensão da diálise peritoneal, o que dificulta seu diagnóstico. Apresentamos o caso de um homem caucasiano de 44 anos de idade que esteve em diálise peritoneal por 6 anos e 3 meses e foi transferido para hemodiálise devido a falha de ultrafiltração. Durante seus últimos meses em diálise peritoneal, ele desenvolveu anorexia e astenia, que foram inicialmente atribuídas à inadequação da diálise. Após a indução de hemodiálise, o paciente desenvolveu dor abdominal, aumento do volume abdominal, obstipação alternada com diarreia, e perda de peso. A tomografia computadorizada mostrou ascite de novo. Foi considerado um diagnóstico de esclerose peritoneal encapsulante precoce, e o tratamento foi prontamente iniciado com suporte nutricional, prednisolona oral e tamoxifeno por um ano. O paciente progrediu com resolução dos sintomas. Um mês após o término do tratamento, ele foi submetido a um transplante renal bem-sucedido e permanece sem maiores intercorrências. Um alto nível de suspeita clínica é crucial para o diagnóstico precoce da esclerose peritoneal encapsulante, uma vez que a doença pode ser fatal em estágios avançados. Este caso destaca que, com tratamento precoce, o transplante renal pode ser realizado com sucesso após um episódio de esclerose peritoneal encapsulante.

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Encapsulating peritoneal sclerosis is an uncommon but serious complication of peritoneal dialysis. In most cases, the symptoms appear after peritoneal dialysis withdrawal, which hampers its diagnosis. We present the case of a 44-years-old Caucasian male who had been on peritoneal dialysis for 6 years and 3 months and was switched to hemodialysis due to ultrafiltration failure. During his last months on peritoneal dialysis, he developed anorexia and asthenia, which were initially attributed to dialysis inadequacy. After hemodialysis induction, the patient developed abdominal pain, increased abdominal volume, obstipation alternating with diarrhea, and weight loss. Computed tomography showed de novo ascites. A diagnosis of early encapsulating peritoneal sclerosis was considered, and treatment was promptly initiated with nutritional support, oral prednisolone, and tamoxifen for one year. The patient progressed with resolution of the symptoms. One month after the end of the treatment, he underwent a successful kidney transplant and remain without any major intercurrences. A high level of clinical suspicion is crucial for the early diagnosis of encapsulating peritoneal sclerosis as the disease can be fatal in advanced stages. This case highlights that with early treatment, kidney transplantation can be successfully performed after an episode of encapsulating peritoneal sclerosis.

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Object recognition memory (ORM) serves to distinguish familiar items from novel ones. Reconsolidation is the process by which active memories are updated. The hippocampus is engaged in ORM reconsolidation through a mechanism involving induction of long-term potentiation (LTP). The transcription factor Zif268 is essential for hippocampal LTP maintenance and has been frequently associated with memory processes. However, its possible involvement in ORM reconsolidation has not been determined conclusively. Using Zif268 antisense oligonucleotides in combination with behavioural, biochemical and electrophysiological tools in rats, we found that hippocampal Zif268 is necessary to update ORM through reconsolidation but not to retrieve it or keep it stored. Our results also suggest that knocking down hippocampal Zif268 during ORM reconsolidation deletes the active recognition memory trace.

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O objetivo do estudo foi avaliar as alterações, após um programa de exercícios aquáticos, dos parâmetros antropométricos e de qualidade de vida relacionada à saúde (QVRS) em crianças com sobrepeso ou obesidade. Trata-se de um ensaio clínico não-randomizado e controlado realizado na cidade de Uberaba, Minas Gerais com participação de 33 crianças com média de idade de 9,10 ± 1,00 anos, de ambos os sexos. As crianças foram alocadas em grupo controle (GC, n = 21) e grupo experimental (GE, n = 12). O GE realizou 21 semanas de exercícios aquáticos de caráter aeróbio recreativo, 54 sessões de 60 minutos cada. No baseline e pós-intervenção, foram avaliados massa corporal, estatura, IMC e QVRS por meio do Questionário Pediátrico de Qualidade de Vida (Pedsql versão 4.0). Observou-se efeito significativo do tempo no aspecto emocional da QVRS relatada pelos filhos (p = 0,009), houve redução dos escores do GE e aumento nos escores do GC. Na dimensão atividade escolar houve tendência para interação tempo*grupo (p = 0,059), em que observamos redução dos escores no GE e aumento no GC após 21 semanas. Em relação à antropometria e QVRS relatada pelos pais, não foram observadas diferenças significativas em ambos os grupos. Em conclusão, houve alterações significativas na dimensão emocional relatado pelas crianças, porém, a intervenção não alterou significativamente a antropometria e demais dimensões da QVRS de crianças com sobrepeso ou obesidade

The aim of this study was to evaluate the changes in anthropometric parameters and health-related quality of life (HRQoL), post aquatic program intervention among overweight and obese children. This non-ran-domized controlled clinical trial was performed in Uberaba, Minas Gerais with 33 children, mean age of 9.10 ± 1.00 years, of both sex. Children were allocated in control group (CG; n = 21) and experimental group (EG; n = 12). The EG performed 21 weeks of the aerobic recreative aquatic exercise, 54 sessions each 60 minutes. At baseline and post intervention, body mass, height, BMI and the HRQoL by Pediatric Quality of life Inventory (Pedsql version 4.0) were assessed. A significant time effect in the emotional aspect to HRQL reported by children (p = 0.009) was observed, that is, there was a score reduction in EG and a score increase in CG. There was tendency to time*group interaction effect (p = 0.059) for the school activities dimension, with a score reduction in EG and score increase in CG after 21 weeks. No significant change was observed in the anthropometry or HRQL reported by parents in both groups. In conclusion, there was significant change in emotional dimension reported by children, however, the intervention did not significantly change the anthropometry and other HRQoL dimensions in overweight or obesity of children

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Abstract The aim of this study was to evaluate the effects of dual task multimodal physical training (MPT) on the cognitive functions and muscle strength in older adults with AD. Participants were 19 subjects with AD in the mild and moderate stages, divided into training group (TG) and control group (CG). The TG performed dual task MPT for 12 weeks. Subjects were evaluated at the pre- and post-intervention moments. The Mini Mental State Examination (MMSE), Clock Drawing Test (CDT) and Frontal Assessment Battery (FAB) were used to assess cognition. For muscle strength, the Chair Lift and Sit Test (CLST) and Manual Grasp Force (MGF) were used. The Wilcoxon test was used to analyze pre and post intragroup moments. The TG showed a significant improvement in FAB and CLST (p≤0.05) and a tendency to improve the MMSE score (p≤0.08). The CG showed significant improvement in CLST (p≤0.05). Dual task MPT improves the frontal cognitive functions and lower limb muscle strength of older adults with AD.

Resumo Objetivou-se avaliar os efeitos do treinamento físico multimodal (TFM) com dupla tarefa nas funções cognitivas e força muscular de idosos com DA. Participaram 19 indivíduos com DA no estágio leve e moderado, divididos em grupo treinamento (GT) e grupo controle (GC). O GT realizou TFM com dupla tarefa por 12 semanas. Os idosos foram avaliados no momento pré e pós-intervenção. Para avaliação da cognição foram utilizados o Mini Exame do Estado Mental (MEEM), Teste do Desenho do Relógio (TDR) e Bateria de Avaliação Frontal (BAF). Para a força muscular o Teste de Levantar e Sentar da Cadeira (TLSC) e Força de Preensão Manual (FPM). O teste de Wilcoxon foi utilizado para analisar os momentos pré e pós-intragrupos. O GT apresentou melhora significativa na BAF e TLSC (p≤0,05) e tendência de melhora no escore do MEEM (p≤0,08). O GC apresentou melhora significativa no TLSC (p≤0,05). O TFM com dupla tarefa melhorou as funções cognitivas frontais e a força muscular de membros inferiores de idosos com DA.

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Objetivo: compreender a percepção de integrantes do Rotary Clube sobre o consumo de bebidas alcoólicas. Métodos: Estudo fenomenológico em Maurice Merleau-Ponty; realizado em duas unidades do Rotary Clube no interior da Bahia, Brasil, nos meses de setembro a dezembro de 2014, com 12 participantes. As descrições vivenciais foram produzidas mediante Grupo Focal e submetidas à técnica analítica da ambiguidade. Resultados: Os participantes revelaram: reconhecer o álcool como uma droga apenas se consumido de forma excessiva, cotidiana e que cause danos à saúde; que o consumo da substância encontra-se naturalizado, ao ponto de passar despercebido diante de vários contextos sociais; e que a mídia tem contribuído para essa naturalização. Conclusão: Trata-se de uma situação preocupante no contexto da saúde, uma vez que situa as pessoas que consomem bebidas alcoólicas de forma “sociável” à margem das políticas de prevenção e enfrentamento, o que torna necessário fomentar debates públicos sobre a temática.

Objective: to understand the perception of Rotary Club members on alcohol consumption. Methods: A phenomenological study in Maurice Merleau-Ponty; conducted in two units of the Rotary Club in Bahia, Brazil, from September to December 2014, with 12 participants. The experiential descriptions were produced by Focus Group and submitted to analytical technique of Ambiguity. Results: The participants revealed: recognize alcohol as a drug only if consumed excessively, everyday and causing damage to health; the consumption of the substance is naturalized to the point to go unnoticed on various social contexts; and that the media has contributed to this naturalization. Conclusion: This is a worrying situation in the context of health as it places the people who consume alcohol in a “sociable” the margins of prevention and coping policies, which makes it necessary to promote public debate on the subject.

Objetivo: comprender la percepción de los miembros del Club Rotary sobre el consumo de alcohol. Métodos: Estudio fenomenológico em Maurice Merleau-Ponty; realizado en dos unidades del Rotary Club em Bahia, Brasil, de septiembre a diciembre de 2014, con 12 participantes. Las descripciones experimentales fueron producidos por el grupo de enfoque y sometidos a la técnica analítica de ambigüedad. Resultados: Los participantes revelaron: reconocer el alcohol como una droga sólo si se consume en exceso, todos los días y causando daños a la salud; el consumo de la sustancia se ha naturalizado hasta el punto de pasar desapercebidos en diversos contextos sociales; y que los medios han contribuido a esta naturalización. Conclusión: Esta es una situación preocupante en el contexto de la salud ya que coloca las personas que consumen alcohol em un “sociables” los márgenes de prevención y políticas de adaptación, lo que hace necesario promover el debate público sobre el tema.

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In this work the development and validation of analytical methodology for determination of copper in sugarcane spirit samples is carried out. The digital image based (DIB) method was applied along with spot test from the colorimetric reaction employing the RGB color model. For the determination of copper concentration, it was used the cuprizone - a bidentate organic reagent - which forms with copper a blue chelate in an alkaline medium. A linear calibration curve over the concentration range from 0.75 to 5.00mgL-1 (r2=0.9988) was obtained and limits of detection and quantification of 0.078mgL-1 and 0.26mgL-1 were acquired, respectively. For the accuracy studies, recovery percentages ranged from 98 to 104% were obtained. The comparison of cooper concentration results in sugar cane spirits using the DIB method and Flame Atomic Absorption Spectrometry as reference method showed no significant differences between both methods, which were performed using the paired t-test in 95% of confidence level. Thus, the spot test method associated with DIB allows the use of devices as digital cameras and smartphones to evaluate colorimetric reaction with low waste generation, practicality, quickness, accuracy, precision, high portability and low-cost.

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Depression is extremely harmful to modern society. Despite its complex spectrum of symptoms, previous studies have mostly focused on the monaminergic system in search of pharmacological targets. However, other neurotransmitter systems have also been linked to the pathophysiology of depression. In this study, we provide evidence for a role of the cholinergic system in depressive-like behavior of female mice. We evaluated mice knockdown for the vesicular acetylcholine transporter (VAChT KD mice), which have been previously shown to exhibit reduced cholinergic transmission. Animals were subjected to the tail suspension and marble burying tests, classical paradigms to assess depressive-like behaviors and to screen for novel antidepressant drugs. In addition, brain levels of serotonin and dopamine were measured by high performance liquid chromatography. We found that female homozygous VAChT KD mice spent less time immobile during tail suspension and buried less marbles, indicating a less depressive phenotype. These differences in behavior were reverted by central, but not peripheral, acetylcholinesterase inhibition. Moreover, female homozygous VAChT KD mice exhibited higher levels of dopamine and serotonin in the striatum, and increased dopamine in the hippocampus. Our study thus shows a connection between depressive-like behaviors and the cholinergic system, and that the latter interacts with the monoaminergic system.

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Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.

A micropropagação é uma alternativa para a produção de plantas de orquídeas em larga escala. Entretanto, este processo apresenta perdas durante a fase de aclimatização. O emprego de prolina exógena na cultura de tecidos vegetais é uma alternativa para reduzir o estresse das plantas na fase de aclimatização. O objetivo da presente pesquisa foi verificar o crescimento de orquídeas em diferentes sistemas de micropropagação com prolina adicionada no meio de cultura. Plantas de Cattleya walkeriana foram obtidas a partir da germinação de sementes em meio de cultura. Sementes foram germinadas em meio MS, adicionado de 20 g. L-1 de sacarose, solidificado com 6 g. L-1 de ágar e pH ajustado para 5,8. Após 5 meses, plântulas com 1 cm de comprimento foram inoculadas nos frascos de cultivo de acordo com os tratamentos, os quais foram compostos por dois sistemas de micropropagação (convencional e ventilação natural) em combinação com prolina (0, 1 e 2 g L-1). O experimento foi conduzido em esquema inteiramente casualizado, constando de um fatorial 2x3, totalizando 6 tratamentos com 5 repetições. O sistema de ventilação natural com o uso de prolina (1 g L-1) promoveu o maior acúmulo de massa seca e melhor controle da perda de água das plantas.

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O objetivo desse estudo foi avaliar a resistência de união - RU ao cisalhamento de um compósito fluido auto aderente em quatro substratos utilizados na técnica indireta, com uma semana e um ano de armazenamento em água destilada. Quarenta e oito amostras (5 mm largura / 15 mm comprimento / 2 mm espessura) foram preparadas em laboratório protético: 12 amostras do(a) cerômero Adoro/AD, cerâmica/leucita Empress Esthetic/EE, cerâmica/zircônia ZirCAD/ZI (Ivoclar Vivadent), mais a liga Fit Cast SB/ME (Talladium do Brasil), sendo as amostras divididas em dois grupos para cada período de tempo (uma semana e um ano). Dois compósitos fluidos (Controle / FF-Filtek Z350XT Flow / 3M ESPE e o auto aderente / DF-Dyad Flow / Kerr) foram unidos aos quatro substratos. Quatro matrizes Tygon foram posicionadas sobre as amostras, as quais foram preenchidas com os compósitos FF e DF (fotopolimerização 20s). As matrizes foram removidas para expor os corpos de prova (12 por grupo) e as amostras foram armazenadas em água destilada/ 37±2 °C por uma semana e um ano. Após estes períodos, cada amostra foi conectada a uma máquina de ensaios para realização dos ensaios de RU a uma velocidade de 1,0 mm/min, até a falha. Os resultados foram analisados por ANOVA e teste de Tukey (p<0,05). Nos ensaios de uma semana, os substratos leucita e metal apresentaram as maiores médias de RU para ambos os compósitos. Nos ensaios de um ano, os substratos não apresentaram diferença estatística nas médias de RU para ambos os compósitos, com exceção da zircônia que apresentou resultado nulo. Os valores médios de RU, no entanto, foram significativamente menores quando comparados com os resultados dos ensaios de uma semana.(AU)

The aim of this study was to evaluate the shear bond strength (SBS) of a self-adhering flowable composite on four substrates used in indirect technique, for a week and one year of storage in distilled water. Forty eight samples (5 mm wide / 15 mm length / 2 mm thick) were prepared at dental prosthetic laboratory: 12 samples of the ceromer Adoro/AD, leucite ceramic IPS Empress Esthetic/EE, zirconia ceramic ZirCAD/ZI (Ivoclar Vivadent) and the metal ceramic alloy Fit Cast SB/ME (Talladium do Brasil). Samples of each substrate were divided into two groups for each time (a week and one year). Two flowable composites (Control/FF-Filtek Z350 XT Flow/3M ESPE, and the self-adhering/DF-Dyad Flow/Kerr) were bonded to four substrates. Four Tygon tubing were positioned over each sample, which were filled in with the composites FF and DF, and visible light-cured for 20s. The tubings were removed to expose the specimens (12 per group) in format of cylinders, and the samples were stored in distilled water at 37 ± 2°C for one week and one year. After these periods, each sample was attached to testing machine and the specimens were submitted to the shear bond strength test at speed of 1.0mm/min, until failure. The results were analyzed by ANOVA and Tukey test (p<0.05). To one week tests, the substrates leucite and metal showed the highest SBS means, to both composites. For the one-year experiments, the substrates did not show differences in the SBS averages for both composites, except for zirconia that showed null result. However, the SBS averages were lower when compared to one week tests.(AU)

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Inputting information to the brain through direct electrical microstimulation must consider how underlying neural networks encode information. One unexplored possibility is that a single electrode delivering temporally coded stimuli, mimicking an asynchronous serial communication port to the brain, can trigger the emergence of different brain states. This work used a discriminative fear-conditioning paradigm in rodents in which 2 temporally coded microstimulation patterns were targeted at the amygdaloid complex. Each stimulus was a binary-coded "word" made up of 10 ms bins, with 1's representing a single pulse stimulus: A-1001111001 and B-1110000111. During 3 consecutive retention tests (i.e., day-word: 1-B; 2-A, and 3-B), only binary-coded words previously paired with a foot-electroshock elicited proper aversive behavior. To determine the neural substrates recruited by the different stimulation patterns, c-Fos expression was evaluated 90 min after the last retention test. Animals conditioned to word-B, after stimulation with word-B, demonstrated increased hypothalamic c-Fos staining. Animals conditioned to word-A, however, showed increased prefrontal c-Fos labeling. In addition, prefrontal-cortex and hypothalamic c-Fos staining for, respectively, word-B- and word-A-conditioned animals, was not different than that of an unpaired control group. Our results suggest that, depending on the valence acquired from previous learning, temporally coded microstimulation activates distinct neural networks and associated behavior.

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