RESUMO
The effect of a cationic ionophore, monensin, on the replication of Mayaro virus in monkey kidney TC7 and Aedes albopictus cells has been studied. Treatment of these cells with 1 micromol/l monensin during infection did not affect the virus protein synthesis but inhibited severely the virus replication. Electron microscopy of the cells infected with Mayaro virus and treated with monensin revealed that the morphogenesis of Mayaro virus was impaired in TC7 but not in A. albopictus cells.
Assuntos
Alphavirus/efeitos dos fármacos , Monensin/farmacologia , Proteínas Virais/efeitos dos fármacos , Replicação Viral/efeitos dos fármacos , Aedes/citologia , Alphavirus/fisiologia , Alphavirus/ultraestrutura , Animais , Linhagem Celular , Células Clonais/microbiologia , Haplorrinos , Rim/citologia , Proteínas Virais/biossínteseRESUMO
Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.