RESUMO
Cell transplantation offers a promising approach in many neurological disorders. Neural stem (NS) cells are potential candidates for cell therapy. The ability to track the grafted cells in the host tissue will refine this therapy. Superparamagnetic iron oxide nanoparticles (SPION) have been suggested as a feasible method, but there is no consensus about its safety. Here we investigated the feasibility of label NS cells with SPION and track by MRI after transplantation into mouse striatum with SPION cells and its therapeutic effects by grafting the cells into mouse striatum. We demonstrated that SPION-labeled NS cells display normal patterns of cellular processes including proliferation, migration, differentiation and neurosphere formation. Transmission electron microscopy reveals SPION in the cytoplasm of the cells, which was confirmed by microanalysis. Neurons and astrocytes generated from SPION-labeled NS cells were able to carry nanoparticles after 7 days under differentiation. SPION-labeled NS cells transplanted into striatum of mice were detected by magnetic resonance imaging (MRI) and microscopy 51 days later. In agreement with others reports, we demonstrated that NS cells are able to incorporate SPION in vitro without altering the stemness, and can survive and be tracked by MRI after they have been grafted into mice striatum.
Assuntos
Rastreamento de Células/métodos , Nanopartículas de Magnetita/química , Células-Tronco Neurais/fisiologia , Animais , Diferenciação Celular , Sobrevivência Celular , Células Cultivadas , Compostos Férricos/metabolismo , Ferro/metabolismo , Imageamento por Ressonância Magnética/métodos , Camundongos , Microscopia Eletrônica de Transmissão/métodos , Células-Tronco Neurais/citologia , Neurônios/fisiologiaRESUMO
Aproximadamente 75 por ciento de los divertículos duodenales yuxtapapilares (DDY) se localizan a 2 cm o menos del ámpula de Vater y se asocian con una elevada incidencia de cálculos vesiculares del colédoco, y a cuadros de pancreatitis recurrente. En un estudio ambipectivo y descriptivo que se realizó en un periodo de 8 años encontramos que de 680 colangiopancreatografías endoscópicas (CPE), 33 pacientes (4.8 por ciento) tiene DDY, la variedad más común fue localizado al borde de la papila en 15 pacientes (45.4 por ciento). El diagnóstico clínico de referencia más frecuente fue ictericia en 19 casos (57.5 por ciento) y cuadros sugestivos de pancreatitis en 11 casos (33.3 por ciento). El hallazgo endoscópico fue la colédocolitiasis en 14 casos (52.5 por ciento) y dilatación del conducto pancreático en 7 (21.2 por ciento). La primera se trato por vía endoscópica satisfactoriamente en 10 casos (30.3 por ciento)