RESUMO
White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD50 of the Mx-H strain was a dilution dose of 10-7.5 ; the mortality titre was 109.2 LD50 per gram. In shrimp injected with 102.5 to 106.5 LD50 , no significant virulence differences were evident. Using mortality data, the four WSSV strains grouped into three virulence levels. The Mx-F strain (intermediate virulence) and the Mx-C strain (high virulence) showed more genetic differences than those observed between the Mx-G (low-virulence) and Mx-H (high-virulence) strains, in ORF94 and ORF125. The application of high-viral-load inocula proved useful in determining the different virulence phenotypes of the WSSV strains from the Eastern Pacific.
Assuntos
Genes Virais/genética , Genótipo , Penaeidae/virologia , Virulência , Vírus da Síndrome da Mancha Branca 1/genética , Vírus da Síndrome da Mancha Branca 1/patogenicidade , Animais , Sequência de Bases , México , Fases de Leitura AbertaRESUMO
To determine the agent responsible for the massive mortalities of the Pacific oyster Crassostrea gigas in northwest Mexico, 30 oysters were sampled after a severe mortality event in 2006 along the Sonoran coast. Histological analyses revealed the presence of a protozoan and Ray's fluid thioglycollate medium (RFTM) assays showed the presence of Perkinsus sp., identified as P. marinus from the DNA sequence of the internal transcribed spacer (ITS) of the ribosomal RNA (rRNA) gene complex. PCR analyses for Marteilia refringens, M. sydneyi, and Haplosporidium costale were negative. P. marinus presence in the Pacific oyster may be responsible for massive mortalities of the oyster, along with other environmental factors in the Gulf of California.
Assuntos
Alveolados/genética , Alveolados/isolamento & purificação , Crassostrea/parasitologia , Animais , DNA Espaçador Ribossômico , Interações Hospedeiro-ParasitaRESUMO
For the shrimp farming industry of Mexico, disease outbreaks caused by white spot syndrome virus (WSSV) are relatively recent. Efforts to control the virus are assisted by monitoring for its prevalence in aquaculture systems, but few attempts have been made to search for it in carriers from coastal waters. To search for WSSV carriers in the Gulf of California, we made surveys off the coast of Sinaloa, Mexico, in March 2001, November 2001, and September 2003 using polymerase chain reaction (PCR) assays and histopathology. WSSV-positive shrimp were detected only in November 2001, after hurricane Julliete. This suggested possible dispersal of WSSV to the marine environment from infected shrimp farms.