RESUMO
Antibiotic bacterial resistant is a huge concern worldwide and probiotics offer an alternative to mitigate it. This study explores Cystobasidium benthicum LR192 as possible probiotic through microbiological and immunological analyses in mouse model. C. benthicum LR192 was isolated from lichens in a hyperarid environment in Baja California Sur, Mexico. First, microbiological analysis was assessed using 1 × 105 CFU/mL in YM broth: resistance to 1% of bile salts and pH of 2, 3 and 5 (control). Then, yeast capacity to adhere onto the intestinal mucosa and safety to mouse splenocytes were tested. Finally, immunological parameters (phagocytic ability, respiratory burst and myeloperoxidase activities, nitric oxide and IgG production) and immune-associated gene expression (IL-1ß, IL-6 and INF-γ) were determined in daily supplemented mice with the yeast (1 × 108 CFU) at days 10 and 15. The results indicate that C. benthicum LR192 has medium resistance to bile salts and low pH, can adhere to the intestine and did not cause cytotoxicity in splenocytes. Immune parameters and immune-related gene expression indicated immunomodulation at day 10 and 15, specially in leucocytes challenged with Escherichia coli. In conclusion, C. benthicum LR192 showed safe potential probiotic properties, but further studies should be performed to confirm it as a probiotic prospect for humans.
Assuntos
Probióticos , Saccharomyces cerevisiae , Humanos , Camundongos , Animais , México , Ácidos e Sais Biliares , Escherichia coliRESUMO
Se presenta el caso de un paciente varón de 80 años de edad con carcinoma Epidermoide de la glándula parótida izquierda infiltrante que recibió tratamiento quirúrgico. Se describe el acto quirúrgico con un resultado final satisfactorio(AU)
It was presented the case of an 80 year- old- male patient with epidermal carcinoma of the infiltrating left parotid gland, who received surgical treatment. The surgical procedure was described with a satisfactory final result(EU)
Assuntos
Humanos , Masculino , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/etiologia , Carcinoma de Células Escamosas/cirurgia , Glândula Parótida/cirurgiaRESUMO
PURPOSE: To evaluate the efficacy of FDG-PET in the definition of tumour lung node lesions and to compare the diagnostic validity of CT and FDGPET in the staging of patients with non-small cell lung cancer (NSCLC). MATERIAL AND METHODS: Patients with clinical suspicion of potentially resectable NSCLC (n = 108) were studied by standard procedures in our setting, including fibrobronchoscopy, transthoracic fine-needle aspiration, thoracoabdominal CT and FDG-PET. PET images were analysed by researchers blinded to results of other imaging modalities. Definitive tumour diagnosis was by histopathological study in patients who underwent surgery and by specific imaging methods and biopsy, when available, in patients who did not. Diagnostic accuracy was evaluated by comparing CT/PET results with the definitive diagnosis. RESULTS: In 13% of patients, no FDG-PET findings were observed and the histological study was negative for tumour. In 22% of patients, FDG-PET detected metastatic disease (M0 by CT). For mediastinal involvement, global diagnostic accuracy was 0.90 with FDG-PET and 0.59 with CT. False positive FDG-PET findings were produced by inflammatory conditions and false negative findings by the small size or proximity of lymph nodes to primary tumour. Mediastinal staging by CT and FDG-PET was correct in 56% and 87% of patients, respectively. CT indicated mediastinal invasion in 17% of patients with no FDG-PET finding of mediastinal involvement. Conversely, mediastinal spread was undetected by CT in 14% of patients with FDG-PET findings of mediastinal involvement. CONCLUSIONS: Although complementary, the functional method (FDG-PET) is significantly superior to the structural method (CT) for detection of mediastinal tumour disease.
Assuntos
Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Fluordesoxiglucose F18 , Neoplasias Pulmonares/diagnóstico , Tomografia por Emissão de Pósitrons , Compostos Radiofarmacêuticos , Tomografia Computadorizada por Raios X , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de NeoplasiasRESUMO
La microalbuminuria es un rasgo de la enfermedad renal incipientes, que tiene valor pronóstico y puede reflejar alteraciones sistémicas del endotelio vascular. Su determinación requiere tecnología fuera del alcance de muchos hospitales en nuestro medio. Se describe un método para medir la excreción urinaria de albúmina con los recursos propios de un laboratorio de hospital general. Pre y posergometría en 17 voluntarios sanos, se determinó la excreción urinaria de albúmina por electroforesis en placa de acetato de celulosa, con elución posterior y medición densitométrica óptica en espectrofotómetro. El método detectó niveles de albuminuria aún por debajo de 20 mgc/min. Hubo una relación directa entre la carga de ejercicio realizado y la excreción urinaria de albúmina. Se propone el método como una alternativa para la detección de microalbuminuria con fines asistenciales y de investigación clínica