RESUMO

To genetically characterize dengue 2 (DEN-2) viruses in Oaxaca, Mexico, the C protein, and a portion of the prM protein genes of 8 isolates from the 2001 DEN epidemic were sequenced. The sequences were compared to those of prototype DEN-2 viruses from various parts of the world. Phylogenetic analysis suggested that the 2001 isolates of DEN-2 were of the American/Asian genotype and were most similar to the Jamaica and Venezuelan isolates MARA3, LARD1996 and LARD1910. Molecular analyses confirmed the origin of the isolates. This study indicates that DEN-2 strains of American/Asian genotype probably from Southeast Asian are circulating in Oaxaca.

Assuntos

Vírus da Dengue/genética , Animais , Linhagem Celular , Dengue/epidemiologia , Dengue/virologia , Vírus da Dengue/isolamento & purificação , Genótipo , Humanos , México/epidemiologia , Filogenia , RNA Viral/isolamento & purificação , Homologia de Sequência de Aminoácidos , Proteínas Virais/genética