RESUMO
Parasitologists from different medical institutions in Havana City carried out the external validation of ENZYMEBA, a diagnostic procedure of intestinal amebiasis developed at the "Pedro Kourí" Institute of Tropical Medicine. To this end, serial faeces specimens from 212 individuals were collected and observed on the microscope (reference test). The ENZYMEBA immunoassay (validation test) was also made. On comparing ENZYMEBA with the microscopic examination, satisfactory indexes of sensitivity and specificity were found. No cross-reactions were detected in faeces specimens, where found other parasites were present, too. Taking into account that only one faeces specimen per patient is enough for the diagnosis of intestinal amebiasis with ENZYMEBA, this procedure may be useful in studies of therapeutical efficacy and prevalence.
Assuntos
Anticorpos Antiprotozoários/análise , Entamoeba histolytica/isolamento & purificação , Fezes/parasitologia , Técnicas Imunoenzimáticas , Animais , Cuba , Estudos de Avaliação como Assunto , Humanos , Reprodutibilidade dos TestesRESUMO
Golden hamster (Mesocricetus auratus) were infected with cysticerci of Taenia solium. Groups of three were bled and killed weekly up to 15 weeks recording the number of implanted adults in the small intestine. A longitudinal study on the antibody response against adult and larvae Ags was carried out, as well as against Ags of H. nana. By ELISA, antibodies against larvae and adult Ags of T. solium were detected since the first week, showing a peak at 3 and 5 weeks respectively. At 14 weeks antibodies levels were very low, which is the time when the parasite is eliminated. The response against Ags of H. nana was very low and disappeared at 4 weeks. By western blot we found that the infected hamsters can distinguish specific stage Ags both in the adult and larvae of T. solium. After 12 weeks only the adult was recognized, the Ags of H. nana were recognize in western blot only during the first week.