RESUMO
The subcutaneous (s.c.) vaccination of DBA/2 mice with 4 weekly doses of 3 x 10(7) living metacyclic forms of T. cruzi, Y strain, obtained from culture in axenic medium and treated for 24 h with actinomycin-D (50 micrograms/10(7) parasites), a drug that promotes an irreversible blockade of the parasite replication, do not induce any detectable degree of humoral and cellular immunosuppression as assessed by a) the production of anti-SRBC antibodies, b) the permanence of delayed cutaneous reaction to T. cruzi antigen, to PPD and DNCB and c) the degree of blastogenic transformation of spleen lymphocytes in the presence of the specific antigen.
Assuntos
Dactinomicina/farmacologia , Tolerância Imunológica/imunologia , Trypanosoma cruzi/imunologia , Vacinação , Animais , Formação de Anticorpos , Antígenos de Protozoários/imunologia , Feminino , Técnica de Placa Hemolítica , Hipersensibilidade Tardia , Imunidade Celular , Ativação Linfocitária/imunologia , Camundongos , Camundongos Endogâmicos DBARESUMO
The main steps of a sensitive and quantitative technique for the simultaneous determination, in the same microplate, of antigen, antibody and specific immune complexes has been described. The assay involved principles of the double sandwich technique used in the "Enzyme-Linked Immunosorbent Assay" (ELISA). The technique proved to be useful for the estimation of antigen, antibody and specific immune complexes in sera of patients with human chronic hepato-intestinal schistosomiasis.
Assuntos
Anticorpos/análise , Complexo Antígeno-Anticorpo/análise , Antígenos de Helmintos/análise , Schistosoma mansoni/imunologia , Esquistossomose mansoni/imunologia , Adolescente , Adulto , Criança , Feminino , Humanos , Técnicas Imunoenzimáticas , MasculinoRESUMO
The pattern of surface density and distribution of Langerhans Cells (LC) in the Swiss Webster (SW) albino mouse is reported. LC were demonstrated using ATP-Pb and their densities evaluated in the skin of anterior and posterior foot-pads, tail, abdomen, back and ear. Cells were counted using a light microscope with a drawing tube by projecting 0.01 mm2 areas at 150X magnification. The distribution and density of LC were quite different in the various regions of SW mouse skin.