RESUMO

Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, Y, and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional (13)C NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving (13)C, (1)H, and (31)P nuclei.

Assuntos

Cápsulas Bacterianas/química , Neisseria meningitidis/química , Ressonância Magnética Nuclear Biomolecular/métodos , Polissacarídeos Bacterianos/química , Cápsulas Bacterianas/imunologia , Configuração de Carboidratos , Isótopos de Carbono , Modelos Lineares , Estrutura Molecular , Neisseria meningitidis/imunologia , Isótopos de Fósforo , Polissacarídeos Bacterianos/imunologia , Prótons , Reprodutibilidade dos Testes