RESUMO

Short peptide tags genetically fused to recombinant proteins have been widely used to facilitate detection or purification without the need to develop specific procedures. In general, an ideal affinity tag would allow the efficient purification of tagged proteins in high yield, without affecting its function. Here, we describe the purification steps to purify a recombinant polyhistidine-tagged glucosyltransferase from Centella asiatica using immobilized metal affinity chromatography.

Assuntos

Cromatografia de Afinidade/métodos , Glucosiltransferases/isolamento & purificação , Metais , Proteínas Recombinantes de Fusão/isolamento & purificação , Glucosiltransferases/genética , Histidina/genética , Metais/química , Proteínas Recombinantes de Fusão/genética