RESUMO
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.(AU)
Assuntos
Paratuberculose , Reação em Cadeia da Polimerase Multiplex , Mycobacterium avium subsp. paratuberculosis , DescontaminaçãoRESUMO
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.
Assuntos
Animais , Mycobacterium avium subsp. paratuberculosis/classificação , Mycobacterium avium subsp. paratuberculosis/isolamento & purificação , Paratuberculose/microbiologia , Doenças dos Ovinos/microbiologia , Argentina , Elementos de DNA Transponíveis , DNA Bacteriano/genética , Reação em Cadeia da Polimerase Multiplex , Mycobacterium avium subsp. paratuberculosis/genética , Polimorfismo de Fragmento de Restrição , Paratuberculose/diagnóstico , Ovinos , Doenças dos Ovinos/diagnósticoRESUMO
We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.