RESUMO
The animals were divided into three groups G1, G2, G3. Semen collected was diluted in Tris-yolk baseand packaged in 0.25 ml straws, a dose of 100 x 106 sperm. Semen samples were distributed in differentequilibrium time protocols. The group 1 (1h), group 2 (2 h), group 3 (3 h). Mean values for motility were19.37%, 16.87% and 16.25% respectively for G1, G2 and G3. To analyze (TTR) which consisted of placing asemen , and incubated at 37°C for a period of 120 minutes. The force sperm motility and sperm were evaluatedby TTR in different time: 0, 60, 120, 180 minutes. The indices obtained from motility and sperm vigor wereevaluated by PROC GLM (SAS, 2001) and proceeded to the 5% Tukey test. This study demonstrated effect theequilibration period, on the viability of goat semen after the freeze-thaw process, in which the semen exposed toless cooling time has a higher sperm viability.(AU)
Assuntos
Animais , Preservação do Sêmen/veterinária , Análise do Sêmen/veterinária , RuminantesRESUMO
In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.(AU)
Assuntos
Animais , Feminino , Recuperação de Oócitos/estatística & dados numéricos , Recuperação de Oócitos/veterinária , Ovinos/embriologia , MatadourosRESUMO
Which was used sixteen samples of adult goats semen collected using an artificial vagina weresubjected to cryopreservation in order to verify the influence of equilibration time at a temperature of 5 °C onthe freezability of goat semen. Were distributed in the different protocols equilibrium time: 1h equilibration time(G1); 2 h equilibration time (G2); 3h equilibration time (G3).After this process the samples were placed inliquid nitrogen vapor , and maintained for 20 min . The end was made the freezing process in cryogenics tank .Thawing was performed in a water bath at 37°C for 30 seconds. The averages for motility were 19.375 %,16.875 % and 16.250 % respectively for G1, G2 and G3. This study demonstrated effect of equilibration period ,on the viability of goat semen after the process of freeze-thaw, where the group of smaller G1 exposure time wasless deleterious effects.(AU)
Assuntos
Animais , Masculino , Criopreservação/métodos , Criopreservação/veterinária , Ruminantes/fisiologia , Comportamento Reprodutivo/fisiologiaRESUMO
Which was used sixteen samples of adult goats semen collected using an artificial vagina weresubjected to cryopreservation in order to verify the influence of equilibration time at a temperature of 5 °C onthe freezability of goat semen. Were distributed in the different protocols equilibrium time: 1h equilibration time(G1); 2 h equilibration time (G2); 3h equilibration time (G3).After this process the samples were placed inliquid nitrogen vapor , and maintained for 20 min . The end was made the freezing process in cryogenics tank .Thawing was performed in a water bath at 37°C for 30 seconds. The averages for motility were 19.375 %,16.875 % and 16.250 % respectively for G1, G2 and G3. This study demonstrated effect of equilibration period ,on the viability of goat semen after the process of freeze-thaw, where the group of smaller G1 exposure time wasless deleterious effects.
Assuntos
Masculino , Animais , Comportamento Reprodutivo/fisiologia , Criopreservação/métodos , Criopreservação/veterinária , Ruminantes/fisiologiaRESUMO
The animals were divided into three groups G1, G2, G3. Semen collected was diluted in Tris-yolk baseand packaged in 0.25 ml straws, a dose of 100 x 106 sperm. Semen samples were distributed in differentequilibrium time protocols. The group 1 (1h), group 2 (2 h), group 3 (3 h). Mean values for motility were19.37%, 16.87% and 16.25% respectively for G1, G2 and G3. To analyze (TTR) which consisted of placing asemen , and incubated at 37°C for a period of 120 minutes. The force sperm motility and sperm were evaluatedby TTR in different time: 0, 60, 120, 180 minutes. The indices obtained from motility and sperm vigor wereevaluated by PROC GLM (SAS, 2001) and proceeded to the 5% Tukey test. This study demonstrated effect theequilibration period, on the viability of goat semen after the freeze-thaw process, in which the semen exposed toless cooling time has a higher sperm viability.
Assuntos
Animais , Análise do Sêmen/veterinária , Preservação do Sêmen/veterinária , RuminantesRESUMO
In order to evaluate the recovery rate of local abattoirs oocyte the metropolitan region of Teresina-PI.Ovaries were used 72 36 pubescent sheep without defined breed (SRD) .The collected ovaries were transportedto the laboratory in thermos physiological saline (0.9% NaCl) heated at 35 ° C plus 30 ug / ml sulphategentamicin in a maximum of two (2) hours, the follicular aspiration needle attached syringe was subsequentlyperformed, the aspirated pellet was deposited on heated Petri dishes previously in hot plate to 37 ° C for theidentification and classification of oocitárias structures. 52 were recovered oocyte cumulus cells and the ewerecovery rate was 1.4. Therefore the low recovery rate can be the result of the female body condition, foodsupply, breeding age and age of the animal.