RESUMO
BACKGROUND: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. PATIENTS AND METHODS: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. RESULTS: Sixty-six samples were obtained from 3 hospitals-42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P = .41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P = .22). A mix of subtypes was found in 4/21 (19%) children. CONCLUSION: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
Assuntos
Infecções por Vírus Epstein-Barr/virologia , Antígenos Nucleares do Vírus Epstein-Barr/genética , Herpesvirus Humano 4/isolamento & purificação , Doença de Hodgkin/virologia , Proteínas Virais/genética , Adolescente , Adulto , Criança , Pré-Escolar , Infecções por Vírus Epstein-Barr/patologia , Feminino , Genótipo , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/imunologia , Doença de Hodgkin/patologia , Humanos , Imuno-Histoquímica , Hibridização In Situ , Masculino , Estudos Retrospectivos , Adulto JovemRESUMO
La fusión espleno-gonadal es una malformación congénita muy rara que consiste en la fusión anormal entre el bazo y una de las gonadas o derivados del mesonefros y sucede aproximadamente a la sexta semana de vida intrauterina. Se ha observado en ambos sexos y en ambos lados, pero predomina por mucho en los varones y en el lado izquierdo. Existen dos tipos de fusión, la continua y la discontinua. Se presenta el caso de una niña de 13 años que ingresó con manifestaciones sugestivas de apendicitis, por lo que fue intervenida quirúrgicamente. El diagnóstico postoperatorio fue de torsión de quiste de ovario derecho; sin embargo el estudio histológico reveló la presencia de tejido gonadal y esplénico en el espécimen analizado