RESUMO
The study aimed to evaluate the effect of powdered coconut water-based diluent (ACP-101c) associated with extra virgin coconut oil (CO) as an external cryoprotectant in the conservation of cryopreserved buck sperm. For cryopreservation, the ejaculates from four bucks were pooled and divided into three aliquots and diluted at 37°C for treatments T1 (ACP-101c + 2.5% egg yolk +7% glycerol), T2 (ACP-101c + 2.5% CO +7% glycerol), and T3 (ACP-101c + 5% CO +7% glycerol). Then, the samples were packaged and cooled at a rate of 1.07°C/min decrease. Upon reaching 4°C, the samples were stored in a refrigerator at 4°C for 30 minutes for stabilization. After this period, the straws were frozen in nitrogen vapor for 15 minutes and then immersed and stored in liquid nitrogen at -196°C. After thawing, the samples were evaluated for sperm kinetics, plasma membrane integrity, acrosomal integrity, membrane functionality, mitochondrial activity (MA), and sperm morphology. In this study, no statistically significant differences were observed between the three treatments regarding the kinetic parameters (p > 0.05; Table 1). However, in relation to the velocities, a reduction was observed beyond the expected. There were no statistically significant differences between the diluents T1, T2, and T3 for the three velocities (curvilinear velocity [VCL], linear velocity [VSL], average path velocity [VAP]). Furthermore, no statistically significant differences were observed (p > 0.05) among treatments regarding the evaluation of membrane integrity, the functional membrane, MA, and sperm morphology after thawing. In conclusion, the use of CO in concentrations of 2.5% and 5.0% is effective in maintaining goat sperm quality, presenting itself as an alternative diluent for international programs of artificial insemination and embryo transfers. [Table: see text].
Assuntos
Preservação do Sêmen , Animais , Óleo de Coco/farmacologia , Criopreservação , Crioprotetores/farmacologia , Glicerol/farmacologia , Cabras , Masculino , Nitrogênio/farmacologia , Motilidade dos Espermatozoides , EspermatozoidesRESUMO
tThe additives of animal origin including egg yolk, widely used for sperm preservation. In order tominimize the impact on the use of animal products, the objective of this study was to evaluate the effect oflyophilized extract of Aloe vera and ACP 102® in ram semen refrigerated at 4°C for 48h. Ejaculates from threeram were split into three aliquots and diluted in ACP®, ACP®/ALV and ALV. Motility and viability was evaluatedafter sperm dilution, 2h, 24h and 48h of storage at 4°C. Results showed that there was a reduction in total andprogressive motility of fresh as compared to other storage time. No differences were observed in viability of freshand 2h as well as there was no difference of semen at for 24h and 48h storage. In conclusion, study showed that theaddition of lyophilized Aloe vera extract was able to maintain semen viability similar to ACP.
Assuntos
Animais , Aloe , Ovinos/embriologia , Preservação do Sêmen/métodos , Liofilização/métodos , Liofilização/veterináriaRESUMO
The objective of this study was to evaluate reproductive parameters of Canindé and British Alpine goatsin the states of Piauí and Ceará by measuring scrotal circumference (CE) and seminal parameters of eightanimals (Canindé (4) and British Alpine (4). Four samples were collected from each animal with the aid of anartificial vagina, after which each ejaculate was evaluated for volume, sperm concentration, total andprogressive mass motility, percentage of motile spermatozoa and vigor. Data were expressed as mean andstandard deviation and analyzed using the statistical program Graphpad Prism®. Although the values ofprogressive motility, scrotal circumference and volume were significantly higher in the British Alpine breed, thetwo races of the present study presented values regarding total motility and vigor within the one recommendedby the CBRA. Thus, the semen of the two breeds can be used for artificial insemination, germplasm bankformation and genetic breeding.
Assuntos
Animais , Análise do Sêmen/veterinária , Fenômenos Reprodutivos Fisiológicos , RuminantesRESUMO
tThe additives of animal origin including egg yolk, widely used for sperm preservation. In order tominimize the impact on the use of animal products, the objective of this study was to evaluate the effect oflyophilized extract of Aloe vera and ACP 102® in ram semen refrigerated at 4°C for 48h. Ejaculates from threeram were split into three aliquots and diluted in ACP®, ACP®/ALV and ALV. Motility and viability was evaluatedafter sperm dilution, 2h, 24h and 48h of storage at 4°C. Results showed that there was a reduction in total andprogressive motility of fresh as compared to other storage time. No differences were observed in viability of freshand 2h as well as there was no difference of semen at for 24h and 48h storage. In conclusion, study showed that theaddition of lyophilized Aloe vera extract was able to maintain semen viability similar to ACP.(AU)
Assuntos
Animais , Ovinos/embriologia , Preservação do Sêmen/métodos , Aloe , Liofilização/métodos , Liofilização/veterináriaRESUMO
The objective of this study was to evaluate reproductive parameters of Canindé and British Alpine goatsin the states of Piauí and Ceará by measuring scrotal circumference (CE) and seminal parameters of eightanimals (Canindé (4) and British Alpine (4). Four samples were collected from each animal with the aid of anartificial vagina, after which each ejaculate was evaluated for volume, sperm concentration, total andprogressive mass motility, percentage of motile spermatozoa and vigor. Data were expressed as mean andstandard deviation and analyzed using the statistical program Graphpad Prism®. Although the values ofprogressive motility, scrotal circumference and volume were significantly higher in the British Alpine breed, thetwo races of the present study presented values regarding total motility and vigor within the one recommendedby the CBRA. Thus, the semen of the two breeds can be used for artificial insemination, germplasm bankformation and genetic breeding.(AU)