RESUMO

The Brucella abortus ribosomal protein L7/L12 is an immunodominant antigen and an interesting candidate for the development of oral live vaccines against brucellosis. Here, a recombinant Lactococcus lactis strain producing L7/L12 under the control of nisin inducible promoter was orally administered to BALB/c mice. Significant levels of anti-L7/L12 specific IgA detected in feces revealed an induced local humoral immune response. However, serum analysis did not reveal any anti-L7/L12 antibodies suggesting the absence of a systemic response. Nevertheless, the vaccinated mice showed a partial protective immunity against B. abortus virulent strain (S2308) challenged by intraperitoneal inoculation.

Assuntos

Antígenos de Bactérias/imunologia , Vacina contra Brucelose/administração & dosagem , Vacina contra Brucelose/imunologia , Brucella abortus/imunologia , Lactococcus lactis/metabolismo , Proteínas Ribossômicas/imunologia , Administração Oral , Animais , Antígenos de Bactérias/genética , Vacina contra Brucelose/genética , Brucella abortus/genética , Brucelose/imunologia , Brucelose/prevenção & controle , Ensaio de Imunoadsorção Enzimática , Lactococcus lactis/genética , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Ribossômicas/genética