RESUMO
Plasma free amino acids were measured in the fasting state and 3 and 4 hours postprandially in children after 9 days consumption of diets in which lupin provided all of 6.4-6.7% protein kcal, either with supplementation of 2% (grams/gram protein) DL-methionine (L & Met, n = 9) or with an isonitrogenous amount of urea (L & U, n = 10). Fasting concentration of total amino acids (TAA) and of essential amino acids (TEAA) and the TEAA:TAA molar ratio did not differ between diets. Fasting Met concentration (15 +/- 4 mumol/liter) and the Met:TEAA ratio (0.021 +/- 0.005) were markedly depressed after 9 days of L & U. Supplementation with Met caused an expected increase of Met concentration (25 +/- 6 mumol/liter) and the Met:TEAA molar ratio (0.039 +/- 0.007) and a profound decrease of Thr concentration (119 +/- 28 to 77 +/- 22 mumol/liter) and Thr:TEAA (0.165 +/- 0.027 to 0.124 +/- 0.028). Postprandially after L ", Met and Met: TEAA did not change from their low fast ing values. After L rMet, Met and Met:TEAA increased significantly relative to fasting values. Threonine concentration increased but the Thr:TEAA ratio decreased significantly (0.124 +/- 0.028 to 0.111 +/- 0.027). These studies confirm Met as the first-limiting amino acid in lupin protein and suggest that Thr becomes limiting when Met is provided in adequate amounts.
Assuntos
Aminoácidos/sangue , Fabaceae , Metionina/farmacologia , Proteínas de Vegetais Comestíveis/metabolismo , Plantas Medicinais , Aminoácidos Essenciais/sangue , Alimentos Fortificados , Humanos , Lactente , Peru , Proteínas de Vegetais Comestíveis/normas , Ureia/farmacologiaRESUMO
Free catecholamine (epinephrine + norepinephrine) excretions of normal male infants 2.1-3.2, 4.5-10.6, and 12.5-18.5 months of age, respectively, and of infants and children with marasmus or marasmic kwashiorkor were measured on three consecutive days after admission and after partial rehabilitation. In normal infants, particularly the older ones, the first day's excretion was higher than that of the next two days, probably in response to the stress of the procedures. There was an increase with age, but on a surface area basis, the differences were not significant, the three age groups excreting 15.1 plus or minus 10.2, 23.8 plus or minus 20.9, and 24.7 plus or minus 14.3 mug/m-2/day, respectively. Excretions of marasmic infants on admission were not significantly different from those of the control children. Higher mean values were due to elevated excretions of infants with severe infection. After partial rehabilitation, excretions were similar to those of control subjects. In children with marasmic kwashiorkor excretions were no different from those of control infants, except in severely infected children. Admission values revealed more day-to-day variation than recovery values or than those of normal and marasmic infants, in whom subject to subject variation was more marked.