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1.
BMC Genomics ; 25(1): 726, 2024 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-39060982

RESUMO

BACKGROUND: A heterozygous-enriched region (HER) is a genomic region with high variability generated by factors such as balancing selection, introgression, and admixture processes. In this study, we evaluated the genomic background of HERs and the impact of different parameters (i.e., minimum number of SNPs in a HER, maximum distance between two consecutive SNPs, minimum length of a HER, maximum number of homozygous allowed in a HER) and scenarios [i.e., different SNP panel densities and whole-genome sequence (WGS)] on the detection of HERs. We also compared HERs characterized in Holstein cattle with those identified in Angus, Jersey, and Norwegian Red cattle using WGS data. RESULTS: The parameters used for the identification of HERs significantly impact their detection. The maximum distance between two consecutive SNPs did not impact HERs detection as the same average of HERs (269.31 ± 787.00) was observed across scenarios. However, the minimum number of markers, maximum homozygous markers allowed inside a HER, and the minimum length size impacted HERs detection. For the minimum length size, the 10 Kb scenario showed the highest average number of HERs (1,364.69 ± 1,483.64). The number of HERs decreased as the minimum number of markers increased (621.31 ± 1,271.83 to 6.08 ± 21.94), and an opposite pattern was observed for the maximum homozygous markers allowed inside a HER (54.47 ± 195.51 to 494.89 ± 1,169.35). Forty-five HER islands located in 23 chromosomes with high Tajima's D values and differential among the observed and estimated heterozygosity were detected in all evaluated scenarios, indicating their ability to potentially detect regions under balancing selection. In total, 3,440 markers and 28 genes previously related to fertility (e.g., TP63, ZSCAN23, NEK5, ARHGAP44), immunity (e.g., TP63, IGC, ARHGAP44), residual feed intake (e.g., MAYO9A), stress sensitivity (e.g., SERPINA6), and milk fat percentage (e.g., NOL4) were identified. When comparing HER islands among breeds, there were substantial overlaps between Holstein with Angus (95.3%), Jersey (94.3%), and Norwegian Red cattle (97.1%), indicating conserved HER across taurine breeds. CONCLUSIONS: The detection of HERs varied according to the parameters used, but some HERs were consistently identified across all scenarios. Heterozygous genotypes observed across generations and breeds appear to be conserved in HERs. The results presented could serve as a guide for defining HERs detection parameters and further investigating their biological roles in future studies.


Assuntos
Heterozigoto , Polimorfismo de Nucleotídeo Único , Sequenciamento Completo do Genoma , Animais , Bovinos/genética , Sequenciamento Completo do Genoma/métodos , Análise de Sequência com Séries de Oligonucleotídeos , Genoma , Genômica/métodos
2.
J Anim Breed Genet ; 2024 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-38807564

RESUMO

Temperament (docility) is a key breeding goal in the cattle industry due to its direct relationship with animal welfare, cattle handler's safety and animal productivity. Over the past six decades, numerous studies have reported heritability estimates for temperament-related traits in cattle populations ranging from low to high values. Therefore, the primary objective of this study was to perform a comprehensive systematic review with meta-analysis to obtain weighted estimates of heritability for temperament-related traits in worldwide cattle populations. After data editing and quality control, 106 studies were included in the systematic review, of which 29.2% and 70.8% reported estimates of heritability for temperament-related traits in dairy and beef cattle populations, respectively. Meta-analyses were performed for 95 heritability estimates using a random model approach. The weighted heritability estimates were as follow: (a) flight score at weaning = 0.23 (95% CI: 0.15-0.32); (b) flight speed at weaning = 0.30 (95% CI: 0.26-0.33); (c) joint analysis of flight speed and flight score at weaning = 0.27 (95% CI: 0.22-0.31); (d) flight speed at yearling = 0.26 (95% CI: 0.21-0.30); (e) joint analysis of flight speed at weaning and yearling = 0.27 (95% CI: 0.24-0.30); (f) movement score = 0.12 (95% CI: 0.08-0.15); (g) crush score at weaning = 0.21 (95% CI: 0.17-0.25); (h) pen score at weaning = 0.27 (95% CI: 0.19-0.34); (i) pen score at yearling = 0.20 (95% CI: 0.17-0.23); (j) joint analysis of pen score at weaning and yearling = 0.22 (95% CI: 0.18-0.26); (k) cow's aggressiveness at calving = 0.10 (95% CI: 0.01-0.19); (l) general temperament = 0.13 (95% CI: 0.06-0.19); (m) milking temperament = 0.16 (95% CI: 0.11-0.21); and (n) joint analysis of general and milking temperament = 0.14 (95% CI: 0.11-0.18). The heterogeneity index ranged from 0% to 77%, and the Q-test was significant (p < 0.05) for four single-trait meta-analyses. In conclusion, temperament is moderately heritable in beef cattle populations, and flight speed at weaning had the highest weighted heritability estimate. Moreover, between-study heterogeneity was low or moderate in beef cattle traits, suggesting reasonable standardization across studies. On the other hand, low-weighted heritability and high between-study heterogeneity were estimated for temperament-related traits in dairy cattle, suggesting that more studies are needed to better understand the genetic inheritance of temperament in dairy cattle populations.

3.
Transl Anim Sci ; 7(1): txad102, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37841322

RESUMO

The decision of premature culling cows directly impacts the profitability of dairy farms. A comprehensive characterization of the primary causes of culling reasons would greatly improve both management and selection objectives in dairy cattle breeding programs. Therefore, this study aimed to analyze the temporal frequencies of 34 culling reasons in Canadian Holstein cows. After data editing and quality control, records from 3,096,872 cows culled from 9,683 herds spread across Canada were used for the analyses covering the periods from 1996 to 2020. Reproductive issues were the main culling reason accounting for 23.02%, followed by milk production (20.82%), health (20.39%), conformation problems (13.69%), economic factors (13.10%), accidents (5.67%), age-related causes (1.67%), and workability (1.63%). Nearly fifty-eight percent of cows were culled after 47 months of age. The observed frequencies of culling due to economic factors were lower than expected from 1996 to 2014 and higher than expected between 2015 and 2020. Reproduction issues had the highest culling frequencies during fall (24.54%), winter (24.02%), and spring (22.51%), while health issues were the most frequent (22.51%) culling reason in the summer season. Health issues (25.50%) and milk production (27.71%) were the most frequent culling reasons in the provinces of Quebec and Ontario, respectively. Reproductive issues showed the highest frequency across climates based on the Köppen climate classification, except for Csb (Dry-summer subtropical or Mediterranean climate) and Bsk (Middle latitude steppe climate), which correspond to small regions in Canada, where production was the most frequent culling reason (29.42% and 21.56%, respectively). Reproductive and milk performance issues were the two main culling reasons in most ecozones, except in Boreal Shield and Atlantic Marine, where health issues had the highest frequencies (25.12 and 23.75%, respectively). These results will contribute to improving management practices and selective decisions to reduce involuntary culling of Holstein cows.

4.
Trop Anim Health Prod ; 54(5): 291, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094617

RESUMO

INTRODUCTION: The genetic groups of native donkeys in Brazil are characterized by adaptation to the local environment. However, the donkey population in the country is declining, mainly because of agricultural mechanization and transportation that has led to the abandonment and the consequent indiscriminate slaughter of these animals. There are three local genetic groups of distinct geographic and temporal formation. However, analyses of their origin, phylogenetic relationship, and population structure are scarce. Within this context, molecular markers such as the mitochondrial control region (D-loop) are useful for these analyses. MATERIAL AND METHODS: This study aimed to evaluate the variation and origin of maternal lineages of groups of naturalized donkeys in Brazil (Brazilian, Nordestino, and Pêga). RESULTS AND DISCUSSION: We detected five mitochondrial haplotypes with 19 polymorphic sites, two of them exclusively found in the Nordestino donkey; this group is in fact more distant from the others. Phylogenetic analysis indicates maternal contributions of two clades (Nubian and Somali) to the formation of the genetic groups of donkeys, a fact that explains the high diversity, structure, and distances of the groups, reported here for the first time. CONCLUSION : This analysis contributes production and conservation of native donkey breeds. It also gives clues about the formation of the Iberian breeds from which Brazilian donkeys originated.


Assuntos
DNA Mitocondrial , Equidae , Animais , Brasil , DNA Mitocondrial/genética , Equidae/genética , Haplótipos , Filogenia
5.
BMC Genomics ; 23(1): 209, 2022 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-35291953

RESUMO

BACKGROUND: A decline in the level of genetic diversity in livestock can result in reduced response to selection, greater incidence of genetic defects, and inbreeding depression. In this context, various metrics have been proposed to assess the level of genetic diversity in selected populations. Therefore, the main goals of this study were to: 1) investigate the population structure of 16 cattle populations from 15 different pure breeds or composite populations, which have been selected for different breeds goals; and, 2) identify and compare runs of homozygosity (ROH) and heterozygosity-enriched regions (HER) based on different single nucleotide polymorphism (SNP) panels and whole-genome sequence data (WGS), followed by functional genomic analyses. RESULTS: A total of 24,187 ROH were found across all cattle populations, with 55% classified in the 2-4 Mb size group. Fourteen homozygosity islands were found in five populations, where four ROH islands located on BTA1, BTA5, BTA16, and BTA19 overlapped between the Brahman (BRM) and Gyr (GIR) breeds. A functional analysis of the genes found in these islands revealed candidate genes known to play a role in the melanogenesis, prolactin signaling, and calcium signaling pathways. The correlations between inbreeding metrics ranged from 0.02 to 0.95, where the methods based on homozygous genotypes (FHOM), uniting of gametes (FUNI), and genotype additive variance (FGRM) showed strong correlations among them. All methods yielded low to moderate correlations with the inbreeding coefficients based on runs of homozygosity (FROH). For the HER, 3576 runs and 26 islands, distributed across all autosomal chromosomes, were found in regions containing genes mainly related to the immune system, indicating potential balancing selection. Although the analyses with WGS did not enable detection of the same island patterns, it unraveled novel regions not captured when using SNP panel data. CONCLUSIONS: The cattle populations that showed the largest amount of ROH and HER were Senepol (SEN) and Montana (MON), respectively. Overlapping ROH islands were identified between GIR and BRM breeds, indicating a possible historical connection between the populations. The distribution and pattern of ROH and HER are population specific, indicating that different breeds have experienced divergent selection processes or different genetic processes.


Assuntos
Objetivos , Endogamia , Animais , Bovinos/genética , Genoma , Genótipo , Homozigoto
6.
Trop Anim Health Prod ; 54(2): 104, 2022 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-35165796

RESUMO

The flaxseed is a nutraceutical food used as a source of α-linolenic acid, which can bring benefits to the health of mammals. This study was carried out to examine the effect of flaxseed inclusion in the diets on the intake of nutrients, body weight, and blood parameters of Alpine goats. Twenty-one adult females with an initial average weight of 41.06 ± 1.84 kg were used in a completely randomized design, with four experimental treatments (0, 5, 10, and 15% of flaxseed in the total diet) and five replications per treatment. The intake of the dry matter presented a decreasing linear effect (P < 0.001), with a reduction of 53.5% between the control diet (0% of flaxseed) and 15% of flaxseed, but no effect was observed on weight gain (P > 0.05). Inclusion of flaxseed from 0 to 15% linearly decreased the intakes of organic matter, crude protein, non-fibrous carbohydrates, and neutral detergent fiber, but increased the ether extract intake (P < 0.001). Regarding plasma concentration traits, increasing the flaxseed levels from 0 to 15% had linear positive effect on LDL, VLDL, and triglycerides (P < 0.05), but no effect on cholesterol, HDL, glucose, creatinine, and urea (P > 0.05). The inclusion of flaxseed in the diet for 80 days changes the nutrients intake and blood parameters but shows no impact on body weight. However, further studies are required to determine the impact of flaxseed on the goat's health in the long term since there has been an increase in the concentration of triglycerides, LDL, and VLDL.


Assuntos
Linho , Ração Animal/análise , Animais , Dieta/veterinária , Digestão , Ingestão de Energia , Feminino , Cabras , Nutrientes
7.
J Anim Breed Genet ; 139(4): 476-487, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35218589

RESUMO

Copy number variations (CNV) are an important source of genetic variation. CNV has been increasingly studied and frequently associated with diseases and productive traits in livestock animals. However, CNV-based genome-wide association studies (GWAS) in Santa Inês sheep, one of the principal sheep breeds in Brazil, have not yet been reported. Thus, the aim of this study was to investigate the association between CNV and growth, efficiency and carcass traits in sheep. The Illumina OvineSNP50 BeadChip array was used to detect CNV in 491 Santa Inês individuals. Then, CNV-based GWAS was performed with a linear mixed model approach considering a genomic relationship matrix, for ten traits: (1) growth: body weight at three (W3) and six (W6) months of age; (2) efficiency: residual feed intake (RFI) and feed efficiency (FE) and (3) carcass: external carcass length (ECL), leg length (LL), carcass yield (CY), commercial cuts weight (CCW), loin eye area (LEA) and subcutaneous fat thickness (SFT). We identified 1,167 autosomal CNV in 438 sheep, with 294 non-redundant CNV, ranging from 21.8 to 861.9 kb, merged into 216 distinct copy number variation regions (CNVRs). One significant CNV segment (pFDR -value<0.05) in OAR3 was associated with CY, while another significant CNV in OAR6 was associated with RFI. Additionally, another 5 CNV segments were considered relevant for investigation in the future studies. The significant segments overlapped 4 QTLs and spanned 8 genes, including the SPAST,TGFA and ADGRL3 genes, involved in cell differentiation and energy metabolism. Therefore, the results of the present study increase knowledge about CNV in sheep, their possible impacts on productive traits, and provide information for future investigations, being especially useful for those interested in structural variations in the sheep genome.


Assuntos
Variações do Número de Cópias de DNA , Estudo de Associação Genômica Ampla , Animais , Estudo de Associação Genômica Ampla/veterinária , Fenótipo , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas , Ovinos/genética
8.
Anim Biotechnol ; 33(2): 201-213, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32633608

RESUMO

Myostatin and MyoD family genes play vital roles in myogenesis and this study aimed to identify association of variants in MyoD1, MyoG, MyF5, MyF6, and MSTN genes with meat traits in Santa Inês sheep. A dataset with 44 variants and records of seven meat traits in 192 lambs (pH0, pH24, a*, b*, L*, tenderness assessed by shear force, and water-holding capacity) was used. Single-locus and haplotype association analyses were performed, and the significance threshold was established according to Bonferroni's method. Single-locus analysis revealed two associations at a Bonferroni level, where the variant c.935-185C > G in MyoD1 had an additive effect (-4.31 ± 1.08 N) on tenderness, while the variant c.464 + 185G > A in MyoG had an additive effect (-2.86 ± 0.64) on a*. Additionally, the haplotype replacement GT>AC in MSTN was associated with pH0 (1.26 ± 0.31), pH24 (1.07 ± 0.27), a* (-1.40 ± 0.51), and tenderness (3.83 ± 1.22 N), while the replacement GT > AG in MyoD1 was associated with pH0 (1.43 ± 0.26), pH24 (1.25 ± 0.22), b* (-1.06 ± 0.39), and tenderness (-4.13 ± 1.16 N). Our results have demonstrated that some variants in MyoG, MyF6, MyoD1, and MSTN can be associated with physicochemical meat traits in Santa Inês sheep.


Assuntos
Carne , Miostatina , Animais , Desenvolvimento Muscular , Miostatina/genética , Fenótipo , Ovinos/genética , Carneiro Doméstico/genética
9.
Rev. bras. saúde prod. anim ; 23: e202200032022, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1388037

RESUMO

This study proposes to investigate the influence of a vitamin-amino acid supplement on the weight of colonies of uruçu stingless bees (Melipona scutellaris). The experiment was carried out with 24 colonies and three treatments, which consisted of a solution of different proportions of supplement (0, 3, and 5 mL) diluted in syrup (water and sugar). Although this supplement is effective and indicated for other species of domestic animals, analysis of variance with repeated measures over time did not reveal a significant effect (P > 0.05) of its dose on the weight of the hives, showing that the supply of the vitamin-amino acid supplement does not meet the nutritional requirements of the colony. The use of this product did not have a positive effect on the development of the uruçu bee colonies, so it should not be employed as a major source of amino acids and vitamins in the diet of bees. Beekeepers are suggested to provide uruçu bees with an abundant diversity of plants so that they have access to different types of pollen as a source of nutrients.(AU)


O objetivo do presente estudo foi verificar a influência do suplemento aminoácido vitamínico no peso de colônias de abelhas sem ferrão Uruçu (Melipona scutellaris). O experimento foi realizado com 24 colônias e três tratamentos, consistindo na oferta de uma solução de xarope (água e açúcar) diluído com diferentes proporções de 0, 3 e 5 mL de suplemento. Embora este suplemento seja eficaz e indicado para outras espécies animais de domésticos, a análise de variância com medida repetida no tempo não apresentou efeito significativo (P> 0,05) do nível desse suplemento sobre o peso das colmeias, mostrando que a oferta do suplemento aminoácido vitamínico, não supre a necessidade nutricional para a colônia. Conclui-se que o uso desse produto não surtiu efeito positivo no desenvolvimento de colônias de abelhas Uruçu (Melipona scutellaris), indicando que não deveria ser utilizado como fonte majoritária de aminoácido e de vitaminas na alimentação das abelhas. Sugere-se que os meliponicultores proporcionem às abelhas Uruçu (Melipona scutellaris) uma abundante diversidade de plantas a fim de que tenham acesso a diferentes tipos de pólen como fonte de nutrientes.(AU)


Assuntos
Animais , Abelhas/fisiologia , Alimentos Fortificados/efeitos adversos , Vitaminas , Aminoácidos , Fenômenos Fisiológicos da Nutrição Animal
12.
J Dairy Res ; 88(1): 16-22, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33593451

RESUMO

Our objective was to evaluate the genetic merit of Holstein cattle population in southern Brazil in response to variations in the regional temperature by analyzing the genotype by environment interaction using reaction norms. Fat yield (FY) and protein yield (PY) data of 67 360 primiparous cows were obtained from the database of the Paraná Holstein Breeders Association, Brazil (APCBRH). The regional average annual temperature was used as the environmental variable. A random regression model was adopted applying mixed models with Restricted Maximum Likelihood (REML) algorithm using WOMBAT software. The genetic merit of the 15 most representative bulls, depending on the temperature gradient, was evaluated. Heritability ranged from 0.21 to 0.27 for FY and from 0.14 to 0.20 for PY. The genetic correlation observed among the environmental gradients proved to be higher than 0.80 for both traits. Slight reranking of bulls for both traits was detected, demonstrating that non-relevant genotype by environment interaction for FY and PY were observed. Consequently, no inclusion of the temperature effect in the model of genetic evaluation in southern Brazilian Holstein breed is required.


Assuntos
Cruzamento , Bovinos/genética , Interação Gene-Ambiente , Genótipo , Leite/química , Animais , Brasil , Bovinos/fisiologia , Indústria de Laticínios , Gorduras/análise , Feminino , Masculino , Proteínas do Leite/análise , Fenótipo , Característica Quantitativa Herdável , Temperatura
13.
Trop Anim Health Prod ; 53(1): 145, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33511501

RESUMO

Beta-casein is a milk protein that has two variants: A1 and A2. Some individuals have difficulties digesting beta-casein A1, which can cause gastrointestinal disorders. A2 milk has emerged as an alternative. This milk only contains beta-casein A2 and is obtained from females carrying the A2A2 genotype of the gene. In cattle, allele and genotype frequencies vary according to breed and marker-assisted selection is performed to obtain A2A2 animals and the consequent production of A2 milk that is easier to digest. This study aimed to evaluate the alleles of beta-casein in buffaloes. A total of 657 buffaloes of four different breeds were genotyped and all animals carried the A2A2 genotype, i.e., allele A1 does not exist in the buffalo species. Thus, all milk products of buffaloes are naturally A2. This result adds value to products derived from buffalo milk.


Assuntos
Búfalos , Caseínas , Animais , Búfalos/genética , Caseínas/genética , Bovinos/genética , Feminino , Genótipo , Leite , Proteínas do Leite
14.
Anim Biosci ; 34(4): 499-505, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32777892

RESUMO

OBJECTIVE: The objective of this study was to evaluate the genetic behavior of a population of Holstein cattle in response to the variation of environmental temperature by analyzing the effects of genotype by environment interaction (GEI) through reaction norms for the somatic cell score (SCS). METHODS: Data was collected for 67,206 primiparous cows from the database of the Paraná Holstein Breeders Association in Brazil, with the aim of evaluating the temperature effect, considered as an environmental variable, distinguished under six gradients, with the variation range found being 17°C to 19.5°C, over the region. A reaction norm model was adopted utilizing the fourth order under the Legendre polynomials, using the mixed models of analysis by the restricted maximum likelihood method by the WOMBAT software. Additionally, the genetic behavior of the 15 most representative bulls was assessed, in response to the changes in the temperature gradient. RESULTS: A mean score of 2.66 and a heritability variation from 0.17 to 0.23 was found in the regional temperature increase. The correlation between the environmental gradients proved to be higher than 0.80. Distinctive genetic behaviors were observed according to the increase in regional temperature, with an observed increase of up to 0.258 in the breeding values of some animals, as well as a reduction in the breeding of up to 0.793, with occasional reclassifications being observed as the temperature increased. CONCLUSION: Non-relevant GEI for SCS were observed in Holstein cattle herds of southern Brazil. Thus, the inclusion of the temperature effect in the model of genetic evaluation of SCS for the southern Brazilian Holstein breed is not required.

15.
Sci. agric. ; 78(3): e20190216, 2021. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-27076

RESUMO

Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one haplotype in LEP were evaluated in haplotype association analysis. The single-locus analysis revealed 23 suggestive additive effects (p < 0.05), but no additive effect was found at the Bonferroni threshold. Haplotype association analysis revealed 19 additive effects, of which ten were at the Bonferroni threshold (p < 0.0074). In IGF1 gene, haplotype replacements were associated with ADG 20.51(7.37), CH 4.09(1.21), WH 3.52(1.20), BL 3.94(1.19), TG 3.88(1.30), TW 1.13(0.36), and LG 3.40(1.08); while in the LEP gene the haplotype replacement was associated with BW100 1.83(0.51), BD −2.51(0.56), and CFS −0.24(0.06). Therefore, there are haplotypes in IGF1 and LEP genes associated with body traits in Santa Inês sheep, which can be useful in marker-assisted selection.(AU)


Assuntos
Animais , Ovinos/genética , Composição Corporal , Haplótipos , Ovinos/crescimento & desenvolvimento , Hormônio do Crescimento/genética
16.
Sci. agric ; 78(3): e20190216, 2021. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1497940

RESUMO

Growth hormone (GH), insulin-like growth factor 1 (IGF1), and leptin (LEP) can be candidate genes for association studies because they play vital roles in the metabolism process. Thus, this study aimed to identify variants in these genes associated with body traits in Santa Inês sheep. The following were recorded: body weight at 100 (BW100) and 240 days (BW240), average daily gain (ADG), withers (WH) and croup (CH) heights, body length (BL), thoracic (TG) and leg (LG) girths, thoracic (TW) and croup (CW) widths, body depth (BD), rib eye area (REA), fat thickness (FT), and carcass finishing score (CFS). Single-locus association analysis was performed with 11 variants in IGF1, 18 in LEP, and 16 in GH. Moreover, two haplotypes in IGF1 and one haplotype in LEP were evaluated in haplotype association analysis. The single-locus analysis revealed 23 suggestive additive effects (p < 0.05), but no additive effect was found at the Bonferroni threshold. Haplotype association analysis revealed 19 additive effects, of which ten were at the Bonferroni threshold (p < 0.0074). In IGF1 gene, haplotype replacements were associated with ADG 20.51(7.37), CH 4.09(1.21), WH 3.52(1.20), BL 3.94(1.19), TG 3.88(1.30), TW 1.13(0.36), and LG 3.40(1.08); while in the LEP gene the haplotype replacement was associated with BW100 1.83(0.51), BD −2.51(0.56), and CFS −0.24(0.06). Therefore, there are haplotypes in IGF1 and LEP genes associated with body traits in Santa Inês sheep, which can be useful in marker-assisted selection.


Assuntos
Animais , Composição Corporal , Haplótipos , Hormônio do Crescimento/genética , Ovinos/crescimento & desenvolvimento , Ovinos/genética
17.
PLoS One ; 15(11): e0242822, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33227032

RESUMO

The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot. Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement. The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW). The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits. The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets. There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan. Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.


Assuntos
Ração Animal , Quitosana/química , Óleo de Sementes de Algodão/química , Manipulação de Alimentos , Animais , Composição Corporal/efeitos dos fármacos , Peso Corporal , Ácidos Graxos Insaturados/química , Humanos , Carne/normas , Carne Vermelha , Ovinos/crescimento & desenvolvimento , Carneiro Doméstico
19.
Rev. bras. zootec ; 49: e20190052, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1443470

RESUMO

The present investigation aimed to evaluate the population structure and inbreeding of Holstein herds in southern Brazil. To carry out the analysis, the Associação Paranaense de Criadores de Bovinos da Raça Holandesa (APCBRH) in Brazil provided the data, which consisted of a pedigree file of 206,796 animals born between 1970 and 2014. Results regarding the following parameters were determined: pedigree integrity, effective number of founders, effective number of ancestors, generation interval, inbreeding coefficient, realized effective population size, and average relatedness coefficient. POPREP and ENDOG v.4.5 software packages were employed to estimate these parameters. Based on the data set, the mean generation interval was found to be 6.3 years, and the average inbreeding coefficient, related to inbred animals, was 4.99%. Furthermore, the realized effective population size varied throughout the time period, ranging from 22 to 114, whereas the rate of inbreeding in this same period showed a decreasing trend towards the later years in the period until 2014. Upon evaluation, average relatedness coefficient was estimated to be 0.71%. Moreover, the effective number of founders and ancestors were estimated as 418 and 400 animals, respectively. According to the level of inbreeding observed, it could be noticed that genetic diversity remains elevated, which will be important to the genetic progress in the Holstein breeding program in Southern Brazil.(AU)


Assuntos
Animais , Bovinos/fisiologia , Endogamia/métodos , Brasil
20.
R. bras. Saúde Prod. Anim. ; 20: e0252019, June 6, 2019. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-19811

RESUMO

The present study endeavors to determine the impact of the inclusion of licuri tart in the diet of finishing lambs, Santa Inês mestizos, by evaluating the economic parameters. For this purpose, a total of 44 lambs, including both uncastrated as well as vaccinated and wormed lambs, with an average age of six months and a mean body weight of 21.2 kg ± 2.7 kg, were employed. Initially, the lambs were fed on the diet which consisted of 40% Tifton-85 hay and 60% of a concentrated mixture, mainly composed of ground corn grain, soybean meal, mineral premix, being further followed by the inclusion of the licuri cake at four different levels (a) 0.0, (b) 8.0,(c) 16.0, and (d) 24% with respect to the dry matter. Principally, the soybean meal and milled corn were replaced by the licuri cake with these levels, thereby constituting four treatments and 11 replicates (11 lambs per treatment) in a completely randomized design. Further, for subjecting the lambs to the above mentioned four different levels of treatment, the lambs were confined for 70 days. The analysis of various economic parameters revealed that upon including the licuri cake up to the level of 24%, the operation cost decreases, which in turn results in higher profitability. However, its cost at the time of use will eventually determine its use for feeding lambs, because of the possible price variations of the ingredients used to formulate the diets along with the model of production of the rural property.(AU)


Objetivou-se determinar o impacto da inclusão datorta de licuri na dieta de cordeiros em terminação, mestiços de Santa Inês, com base na analise econômica.Utilizou-se 44 cordeiros, não-castrados, vacinados e vermifugados, com média de seis meses de idade e peso corporal médio de 21,2 kg ± 2,7 Kg. Os cordeiros foram alimentados com 40% feno de Tifton-85 e 60% de uma mistura concentrada composta por grão de milho moído, farelo de soja, premix mineral e inclusão de torta de licuri nos níveis 0; 8; 16 e 24% com base na matéria seca em substituição ao farelo de soja egrão de milho moído, com esses níveis constituindo os tratamentos, utilizando-se 11 cordeiros por tratamento em delineamento inteiramente casualizado. Os animais foram confinados por 70 dias. O menor custo operacional e consequente maior lucratividade quando se incluiu a torta de licuri até o nível de 24%. No entanto, a decisão de seu emprego na alimentação de cordeiros vai depender de seu custo no momento da utilização, considerando as possíveis variações de preços dos ingredientes utilizados para formulação das dietas e modelode produção da propriedade rural.(AU)


Assuntos
Animais , Ovinos/metabolismo , Arecaceae , Ração Animal , Aumento de Peso , Dieta/veterinária
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