RESUMO
Abstract Length and weight relationships and descriptive statistics are reported for six shrimp species caught off the Madeira archipelago between 1983 and 2014 using bottom and floating traps from depths ranging from 50 to 1,300 m. The parameter b ranged between 2.36 for Plesionika ensis and 2.97 for Plesionika williamsi. All species showed a pattern of negative allometric growth. To the authors' knowledge all weight-length relationships presented herein are recorded for the first time from the Madeira area, and in the cases of Ligur ensiferus and Plesionika ensis are the first references worldwide.
Resumo As relações peso-comprimento e a estatística descritiva foram estimadas para seis espécies de camarões, capturados ao largo do arquipélago da Madeira, entre 1983 e 2014, usando armadilhas de fundo e alvoradas entre os 50 e 1.300 metros de profundidade. O parâmetro b variou entre 2,36 para Plesionika ensis e 2,97 para Plesionika williamsi. Todas as espécies evidenciaram um padrão de crescimento alométrico negativo. De acordo com o conhecimento dos autores, todas as relações peso-comprimento são aqui apresentadas pela primeira vez para a área da Madeira e no caso de Ligur ensiferus e Plesionika ensis são a primeira referência mundial.
Assuntos
Animais , Masculino , Feminino , Decápodes/fisiologia , Portugal , Peso Corporal , Oceano Atlântico , Pandalidae/fisiologia , Tamanho CorporalRESUMO
Abstract Analysis of abundance and population structure of Plesionika narval was performed on data concerning 5,255 specimens obtained from 62 fishing sets carried out off the Madeira archipelago (Northeastern Atlantic) between 2004 and 2008 in a depth range from 101 to 350 m. Abundance ranged from 0.01 to 19.74 specimens-per-trap and significant differences were found between seasons, probably as a result of an increment of population in the spring during the recruitment season. The analysis of size distribution revealed that the carapace length (CL) ranged from 2.45 to 28.61 mm and that mean female size consistently exceeded that of males. Differences in mean CL were statistically significant between depth strata and seasons. Of the specimens sampled, 57.00% were males, 41.88% females and 1.42% undetermined. Sex ratio also differed significantly between seasons according to depth strata, consolidating the hypothesis of the existence of seasonal migrations related with the reproductive cycle of this species. Ovigerous females showed larger sizes and occurred all year around and remain in shallow waters in winter, summer and autumn and move to deeper waters in spring. The highest frequency of ovigerous females was recorded in summer, between 151 and 200 m deep supporting the hypothesis that spawning of this species occurs in shallow waters, especially in late summer.
Resumo O estudo de abundância e dinâmica populacional de Plesionika narval foi efetuado com 5.255 espécimes provenientes de 62 lances de pesca realizados ao largo do arquipélago da Madeira (Atlântico Nordeste) de 2004 a 2008 entre 101 e 350 m de profundidade. A abundância oscilou entre 0,01 e 19,74 espécimes-por-armadilha com diferenças significativas entre estações provavelmente devido ao aumento da população na primavera durante o período de recrutamento. A análise da distribuição de tamanhos revelou que o comprimento da carapaça (CL) variou entre 2,45 e 28,61 mm e que o tamanho médio das fêmeas excedeu o dos machos. Verificaram-se diferenças significativas no CL médio entre estrato de profundidade e estação. Do total amostrado, 57,00% foram machos, 41,88% fêmeas e 1,42% indeterminados. A razão de sexos também diferiu significativamente entre estações de acordo com a profundidade, consolidando a hipótese da existência de migrações sazonais relacionadas com o ciclo reprodutivo desta espécie. As fêmeas ovadas apresentaram maiores tamanhos e ocorreram durante todo o ano permanecendo em águas pouco profundas no inverno, verão e outono e migrando para águas profundas na primavera. A maior frequência de fêmeas ovadas foi registada no verão, entre 151 e 200 m de profundidade reforçando a hipótese de que a desova desta espécie ocorre em águas menos profundas, especialmente no final do verão.
Assuntos
Animais , Masculino , Feminino , Pandalidae/fisiologia , Distribuição Animal , Portugal , Reprodução , Estações do Ano , Razão de Masculinidade , Oceano Atlântico , Dinâmica Populacional , Tamanho CorporalRESUMO
Analysis of abundance and population structure of Plesionika narval was performed on data concerning 5,255 specimens obtained from 62 fishing sets carried out off the Madeira archipelago (Northeastern Atlantic) between 2004 and 2008 in a depth range from 101 to 350 m. Abundance ranged from 0.01 to 19.74 specimens-per-trap and significant differences were found between seasons, probably as a result of an increment of population in the spring during the recruitment season. The analysis of size distribution revealed that the carapace length (CL) ranged from 2.45 to 28.61 mm and that mean female size consistently exceeded that of males. Differences in mean CL were statistically significant between depth strata and seasons. Of the specimens sampled, 57.00% were males, 41.88% females and 1.42% undetermined. Sex ratio also differed significantly between seasons according to depth strata, consolidating the hypothesis of the existence of seasonal migrations related with the reproductive cycle of this species. Ovigerous females showed larger sizes and occurred all year around and remain in shallow waters in winter, summer and autumn and move to deeper waters in spring. The highest frequency of ovigerous females was recorded in summer, between 151 and 200 m deep supporting the hypothesis that spawning of this species occurs in shallow waters, especially in late summer.
Assuntos
Distribuição Animal , Pandalidae/fisiologia , Animais , Oceano Atlântico , Tamanho Corporal , Feminino , Masculino , Dinâmica Populacional , Portugal , Reprodução , Estações do Ano , Razão de MasculinidadeRESUMO
Length and weight relationships and descriptive statistics are reported for six shrimp species caught off the Madeira archipelago between 1983 and 2014 using bottom and floating traps from depths ranging from 50 to 1,300 m. The parameter b ranged between 2.36 for Plesionika ensis and 2.97 for Plesionika williamsi. All species showed a pattern of negative allometric growth. To the authors' knowledge all weight-length relationships presented herein are recorded for the first time from the Madeira area, and in the cases of Ligur ensiferus and Plesionika ensis are the first references worldwide.
Assuntos
Decápodes/fisiologia , Animais , Oceano Atlântico , Tamanho Corporal , Peso Corporal , Feminino , Masculino , Pandalidae/fisiologia , PortugalRESUMO
Analysis of abundance and population structure of Plesionika narval was performed on data concerning 5,255 specimens obtained from 62 fishing sets carried out off the Madeira archipelago (Northeastern Atlantic) between 2004 and 2008 in a depth range from 101 to 350 m. Abundance ranged from 0.01 to 19.74 specimens-per-trap and significant differences were found between seasons, probably as a result of an increment of population in the spring during the recruitment season. The analysis of size distribution revealed that the carapace length (CL) ranged from 2.45 to 28.61 mm and that mean female size consistently exceeded that of males. Differences in mean CL were statistically significant between depth strata and seasons. Of the specimens sampled, 57.00% were males, 41.88% females and 1.42% undetermined. Sex ratio also differed significantly between seasons according to depth strata, consolidating the hypothesis of the existence of seasonal migrations related with the reproductive cycle of this species. Ovigerous females showed larger sizes and occurred all year around and remain in shallow waters in winter, summer and autumn and move to deeper waters in spring. The highest frequency of ovigerous females was recorded in summer, between 151 and 200 m deep supporting the hypothesis that spawning of this species occurs in shallow waters, especially in late summer.(AU)
O estudo de abundância e dinâmica populacional de Plesionika narval foi efetuado com 5.255 espécimes provenientes de 62 lances de pesca realizados ao largo do arquipélago da Madeira (Atlântico Nordeste) de 2004 a 2008 entre 101 e 350 m de profundidade. A abundância oscilou entre 0,01 e 19,74 espécimes-por-armadilha com diferenças significativas entre estações provavelmente devido ao aumento da população na primavera durante o período de recrutamento. A análise da distribuição de tamanhos revelou que o comprimento da carapaça (CL) variou entre 2,45 e 28,61 mm e que o tamanho médio das fêmeas excedeu o dos machos. Verificaram-se diferenças significativas no CL médio entre estrato de profundidade e estação. Do total amostrado, 57,00% foram machos, 41,88% fêmeas e 1,42% indeterminados. A razão de sexos também diferiu significativamente entre estações de acordo com a profundidade, consolidando a hipótese da existência de migrações sazonais relacionadas com o ciclo reprodutivo desta espécie. As fêmeas ovadas apresentaram maiores tamanhos e ocorreram durante todo o ano permanecendo em águas pouco profundas no inverno, verão e outono e migrando para águas profundas na primavera. A maior frequência de fêmeas ovadas foi registada no verão, entre 151 e 200 m de profundidade reforçando a hipótese de que a desova desta espécie ocorre em águas menos profundas, especialmente no final do verão.(AU)
Assuntos
Animais , Palaemonidae/anatomia & histologia , Palaemonidae/crescimento & desenvolvimento , Composição Corporal , Dinâmica PopulacionalRESUMO
Length and weight relationships and descriptive statistics are reported for six shrimp species caught off the Madeira archipelago between 1983 and 2014 using bottom and floating traps from depths ranging from 50 to 1,300 m. The parameter b ranged between 2.36 for Plesionika ensis and 2.97 for Plesionika williamsi. All species showed a pattern of negative allometric growth. To the authors knowledge all weight-length relationships presented herein are recorded for the first time from the Madeira area, and in the cases of Ligur ensiferus and Plesionika ensis are the first references worldwide.(AU)
As relações peso-comprimento e a estatística descritiva foram estimadas para seis espécies de camarões, capturados ao largo do arquipélago da Madeira, entre 1983 e 2014, usando armadilhas de fundo e alvoradas entre os 50 e 1.300 metros de profundidade. O parâmetro b variou entre 2,36 para Plesionika ensis e 2,97 para Plesionika williamsi. Todas as espécies evidenciaram um padrão de crescimento alométrico negativo. De acordo com o conhecimento dos autores, todas as relações peso-comprimento são aqui apresentadas pela primeira vez para a área da Madeira e no caso de Ligur ensiferus e Plesionika ensis são a primeira referência mundial.(AU)
Assuntos
Animais , Pandalidae/anatomia & histologia , Pandalidae/crescimento & desenvolvimento , Pesos e Medidas Corporais/veterináriaRESUMO
Abstract Several experimental surveys were carried out in the Northeastern Atlantic, Madeira archipelago from 1991 to 2008 to explore new fisheries resources. This study examined the selectivity of bottom and floating traps and the analysis of yield-per-recruit (YPR) and biomass-per-recruit (BPR) providing helpful insight to the management of the shrimp Plesionika narval. A total of 28,262 specimens were sampled and the analysis of length at first capture returned higher values when using floating traps indicating that these traps are more selective, exerting less pressure on the resource. The YPR and BPR analysis showed that the stock is under exploited for the studied area and suggests that the use of floating traps in the commercial fisheries of P. narval is recommended, which will allow a higher maximum allowable limit of exploitation and greater yield. The results suggest that P. narval has the potential to support a viable and sustainable fishery using floating traps.
Resumo Vários cruzeiros de investigação foram realizados no Atlântico Nordeste, arquipélago da Madeira entre 1991 e 2008 visando a exploração de novos recursos pesqueiros. O presente estudo contemplou a comparação da seletividade de pesca entre armadilhas de fundo e flutuantes e a análise de rendimento por recruta (YPR) e biomassa por recruta (BPR) facultando informação essencial para a gestão do camarão Plesionika narval. Um total de 28,262 especimens foram amostrados e a análise de comprimento de primeira captura retornou valores mais elevados aquando da utilização de armadilhas flutuantes, indicando que essas armadilhas são mais seletivas e exercem menos pressão sobre o recurso. A análise YPR e BPR mostrou que o estoque se encontra sob explorado para a área de estudo e recomenda a utilização de armadilhas flutuantes na pesca comercial de P. narval, que permitirão um limite máximo de exploração com rendimento superior. Os resultados sugerem que P. narval tem o potencial para suportar uma pescaria viável e sustentável mediante a utilização de armadilhas flutuantes.
Assuntos
Animais , Masculino , Feminino , Conservação dos Recursos Naturais , Pandalidae , Pesqueiros , Portugal , Características de História de VidaRESUMO
Several experimental surveys were carried out in the Northeastern Atlantic, Madeira archipelago from 1991 to 2008 to explore new fisheries resources. This study examined the selectivity of bottom and floating traps and the analysis of yield-per-recruit (YPR) and biomass-per-recruit (BPR) providing helpful insight to the management of the shrimp Plesionika narval. A total of 28,262 specimens were sampled and the analysis of length at first capture returned higher values when using floating traps indicating that these traps are more selective, exerting less pressure on the resource. The YPR and BPR analysis showed that the stock is under exploited for the studied area and suggests that the use of floating traps in the commercial fisheries of P. narval is recommended, which will allow a higher maximum allowable limit of exploitation and greater yield. The results suggest that P. narval has the potential to support a viable and sustainable fishery using floating traps.
Assuntos
Conservação dos Recursos Naturais , Pesqueiros , Características de História de Vida , Pandalidae , Animais , Feminino , Masculino , PortugalRESUMO
The Finite Element Method is a well-known technique, being extensively applied in different areas. Studies using the Finite Element Method (FEM) are targeted to improve cardiac ablation procedures. For such simulations, the finite element meshes should consider the size and histological features of the target structures. However, it is possible to verify that some methods or tools used to generate meshes of human body structures are still limited, due to nondetailed models, nontrivial preprocessing, or mainly limitation in the use condition. In this paper, alternatives are demonstrated to solid modeling and automatic generation of highly refined tetrahedral meshes, with quality compatible with other studies focused on mesh generation. The innovations presented here are strategies to integrate Open Source Software (OSS). The chosen techniques and strategies are presented and discussed, considering cardiac structures as a first application context.
RESUMO
OBJECTIVES: The aim of this work is to provide the first report of a transfusion-acquired HIV-1 infection and to verify transmission from the donor to the recipients using phylogenetic analysis of HIV-1 DNA sequences in a Brazilian blood bank. BACKGROUND: Although haemovigilance procedures based on phylogenetic analysis of HIV have been reported in several countries, this type of study has yet to be conducted in Latin America. MATERIALS AND METHODS: Upon identifying a HIV-1-positive repeat blood donor by enzyme immunoassay (EIA) blood screening, all recipients of the donor's previous donation were identified and tested for HIV-1 by EIA, nucleic acid amplification test and HIV-1 DNA sequencing and phylogenetic analysis. RESULTS: One of the recipients tested positive for HIV-1. The phylogenetic analysis showed a high genetic similarity among the viruses, thus supporting the hypothesis of transmission from the donor to the recipient. CONCLUSIONS: Phylogenetic analysis of HIV-1 DNA sequences has been a decisive tool in verifying suspected transmission of the virus from blood donor to recipient in Brazil.
Assuntos
Bancos de Sangue , Doadores de Sangue , Segurança do Sangue , Transfusão de Sangue , Patógenos Transmitidos pelo Sangue , DNA Viral/genética , Infecções por HIV , HIV-1/genética , Filogenia , Adulto , Brasil , Feminino , Infecções por HIV/genética , Infecções por HIV/transmissão , HumanosRESUMO
The Jarisch-Herxheimer reaction (JHR) is a syndrome observed after antimicrobial treatment of some infectious diseases. The syndrome has clinical characteristics of an inflammatory reaction to antibiotic treatment. A prospective study of patients with a clinical and laboratory diagnosis of syphilis was conducted at a sexually transmitted diseases clinic in Rio de Janeiro, Brazil. Patients were treated with benzathine penicillin and observed for the JHR. A total of 115 patients were included in this study. Fifty-one patients (44%) had secondary syphilis; 37 (32%), primary; 26 (23%), latent; and one (1%), tertiary syphilis. Ten patients (9%) developed the JHR. All JHRs occurred in patients with secondary and latent syphilis. No patients experienced an allergic reaction to penicillin. The JHR occurred less frequently than in previous studies. It is important that health-care professionals recognize the clinical characteristics of the JHR so that it is not misinterpreted as an allergic reaction to penicillin.
Assuntos
Antibacterianos/administração & dosagem , Antibacterianos/efeitos adversos , Inflamação/induzido quimicamente , Penicilina G Benzatina/administração & dosagem , Penicilina G Benzatina/efeitos adversos , Sífilis/tratamento farmacológico , Adolescente , Adulto , Brasil , Feminino , Humanos , Masculino , Estudos Prospectivos , Adulto JovemRESUMO
Vaccine approaches to infectious diseases are widely applied and appreciated. Amongst them, vectors based on recombinant viruses have shown great promise and play an important role in the development of new vaccines. Many viruses have been investigated for their ability to express proteins from foreign pathogens and induce specific immunological responses against these antigens in vivo. Generally, gene-based vaccines can stimulate potent humoral and cellular immune responses and viral vectors might be an effective strategy for both the delivery of antigen-encoding genes and the facilitation and enhancement of antigen presentation. In order to be utilized as a vaccine carrier, the ideal viral vector should be safe and enable efficient presentation of required pathogen-specific antigens to the immune system. It should also exhibit low intrinsic immunogenicity to allow for its re-administration in order to boost relevant specific immune responses. Furthermore, the vector system must meet criteria that enable its production on a large-scale basis. Several viral vaccine vectors have thus emerged to date, all of them having relative advantages and limits depending on the proposed application, and thus far none of them have proven to be ideal vaccine carriers. In this review we describe the potential, as well as some of the foreseeable obstacles associated with viral vaccine vectors and their use in preventive medicine.
Assuntos
Vetores Genéticos/imunologia , Vacinas de DNA/imunologia , Vacinas Virais/imunologia , Viroses/prevenção & controle , Adenoviridae/imunologia , Alphavirus/imunologia , Herpesviridae/imunologia , Humanos , Poliovirus/imunologia , Poxviridae/imunologia , Recombinação Genética , Vacinas Virais/genética , Viroses/genética , Viroses/imunologiaRESUMO
Vaccine approaches to infectious diseases are widely applied and appreciated. Amongst them, vectors based on recombinant viruses have shown great promise and play an important role in the development of new vaccines. Many viruses have been investigated for their ability to express proteins from foreign pathogens and induce specific immunological responses against these antigens in vivo. Generally, gene-based vaccines can stimulate potent humoral and cellular immune responses and viral vectors might be an effective strategy for both the delivery of antigen-encoding genes and the facilitation and enhancement of antigen presentation. In order to be utilized as a vaccine carrier, the ideal viral vector should be safe and enable efficient presentation of required pathogen-specific antigens to the immune system. It should also exhibit low intrinsic immunogenicity to allow for its re-administration in order to boost relevant specific immune responses. Furthermore, the vector system must meet criteria that enable its production on a large-scale basis. Several viral vaccine vectors have thus emerged to date, all of them having relative advantages and limits depending on the proposed application, and thus far none of them have proven to be ideal vaccine carriers. In this review we describe the potential, as well as some of the foreseeable obstacles associated with viral vaccine vectors and their use in preventive medicine.
Assuntos
Humanos , Vetores Genéticos/imunologia , Vacinas de DNA/imunologia , Vacinas Virais/imunologia , Viroses/prevenção & controle , Adenoviridae/imunologia , Alphavirus/imunologia , Herpesviridae/imunologia , Poliovirus/imunologia , Poxviridae/imunologia , Recombinação Genética , Vacinas Virais/genética , Viroses/genética , Viroses/imunologiaRESUMO
Gp43, the major 43-kDa antigenic glycoprotein of Paracoccidioides brasiliensis, or its 15-amino acid inner peptide (P10), induces a T-CD4(+), Th1 cellular immune response which protects BALB/c mice from intratracheal infection by virulent yeast forms. We investigated whether DNA vaccination using the gp43 gene could elicit protective immunity against P. brasiliensis. Animals immunised intramuscularly (i.m.) or intradermally (i.d.) with plasmid DNA containing the gp43 gene induced a specific, long lasting humoral and cellular immune response. A mixed Th1/Th2 cellular immune response in DNA-immunized mice was modulated in vivo by IFN-gamma and was protective in BALB/c mice. A significant decrease in the lung colony forming units (CFUs) and reduced, or no dissemination to the spleen and liver of immunised mice were observed.
Assuntos
Antígenos de Fungos/imunologia , Vacinas Fúngicas/imunologia , Paracoccidioides/imunologia , Vacinas de DNA/imunologia , Animais , Antígenos de Fungos/genética , Células COS , Feminino , Imunização , Interferon gama/fisiologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos BALB C , Paracoccidioidomicose/patologia , Paracoccidioidomicose/prevenção & controleRESUMO
An antigen of apparent molecular mass of 30 kDa, termed p30, was purified from Leishmania (L.) chagasi amastigotes after separation of parasite extracts by sodium dodecyl sulfate-polyacrylamide gel eletroctrophoresis followed by electroelution. The use of the purified antigen in lymphocyte cultures from BALB/c mice previously immunised with L. (L.) chagasi amastigotes led to high levels of proliferation. Animal immunisation with p30 plus complete Freund's adjuvant either by subcutaneous or intraperitoneal route led to comparable antigenic stimulation. Similar stimulation indices induced by p30 were also obtained when animals were immunised with Corynebacterium parvum as adjuvant by the intraperitoneal route. Detection of IL-2 and IFN-gamma in the supernatants from lymphocytes stimulated by p30 and inhibition of the production of these lymphokines in the presence of anti-CD4 strongly indicated the involvement of the Th1 subset in the responses elicited by p30 antigen. Immunisation of BALB/c mice with p30 provided partial protection against challenge with L. (L.) chagasi amastigotes, indicating a protective role for p30 and that Th1 can be related to accquired resistance to visceral leishmaniasis in a murine model. Further characterisation studies were performed by the use of a monoclonal antibody directed to a cysteine proteinase of 30 kDa from L. (L.) amazonensis amastigotes. Despite the cross-reactivity presented by p30 from both Leishmania species, the p30 from L. (L.) chagasi amastigotes lacks proteolytic activity.
Assuntos
Antígenos de Protozoários/isolamento & purificação , Interações Hospedeiro-Parasita , Leishmania/química , Animais , Antígenos de Protozoários/química , Western Blotting , Eletroforese em Gel de Poliacrilamida , Feminino , Leishmania/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Peso Molecular , Células Th1/parasitologiaRESUMO
Lymphoproliferative responses to an antigen from Leishmania amazonensis amastigotes with an apparent molecular mass of 30 kDa, termed p30, were evaluated with BALB/c mice. The p30 antigen was purified after separation of parasite extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroelution. Lymphoproliferative responses to p30 were obtained by subcutaneous immunization of animals with L. amazonensis amastigote extracts, and maximal stimulation indices were observed at an antigen concentration of 5 microg/ml. Induction of lymphoproliferation by p30 is stage specific, and no differences in the responses to this antigen between mice susceptible and resistant to L. amazonensis were detected. The predominant T cells characterized in the lymphocyte cultures were CD4+. Lymphokine analysis of the supernatants from these cultures indicated that Th1 is the subset involved in the lymphoproliferative responses to the antigen. BALB/c mice immunized with p30 and challenged with L. amazonensis amastigotes showed a very low level of infection, indicating a protective role for p30 and a correlation between Th1 and protection. Further biochemical characterization studies showed that this antigen presents cysteine proteinase activity.