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IUBMB Life ; 54(1): 19-25, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12387571

RESUMO

Current literature concerning smooth muscle blood vessels has shown versican as the main proteoglycan (PG) component of the matrix. To show whether smooth muscle matrix has the same PG distribution when present in organs, other than the blood vessels, the inner circular smooth muscle layer of the small intestine was obtained by dissection as a highly purified tissue and analyzed by biochemical and cytochemical methods. The smooth muscle layer PGs were extracted from dog small intestine with 4 M guanidine-HCl in the presence of proteinase inhibitors, purified by charge equilibrium, isolated by equilibrium CsCl density gradients, and analyzed in terms of anion exchange, size, and glycosaminoglycan (GAG) distribution. Proteoheparan sulfate itself represented 91.5% of the PGs present in this tissue. The remainder was proteodermatan sulfate. Cytochemical analyses using the cationic dye cuprolinic blue associated with enzymatic treatments with chondroitinases ABC and heparitinase III showed the arrangement and identification of PGs in basal lamina and intramuscular connective tissues. The PGs in the basal lamina were proteoheparan sulfate, and those associated with collagen fibrils in the endomysium and perimysium were rich in dermatan sulfate. In contrast to the blood vessels, inner circular muscle smooth tissue in intestine has, as the main PG, perlecan.


Assuntos
Dermatan Sulfato/análogos & derivados , Matriz Extracelular/química , Intestino Delgado/química , Músculo Liso/química , Proteoglicanas/análise , Animais , Centrifugação com Gradiente de Concentração , Cromatografia por Troca Iônica , Dermatan Sulfato/análise , Cães , Eletroforese em Gel de Ágar , Proteoglicanas de Heparan Sulfato/análise , Coloração e Rotulagem
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