RESUMO
BACKGROUND: Hematopoietic progenitor cell transplantation is considered a standard-of-care treatment for defined hematological and non-hematological conditions affecting bone marrow-derived cells. METHODS: Patients and potential donors are HLA typed for their HLA-A, -B, -C, -DRB1, and -DQB1 alleles. The best allogeneic donor is one for which each allele matches the patient at HLA-A, -B, -C, and -DRB1 (8/8). For patients with no related donor, the transplant physician will start a search for unrelated donors. The search is performed through a local registry and often includes the search for donors worldwide. The Argentinean HPC Donors Registry was established in 2003. Our National HPC Donor Registry has already typed more than 31,000 donors for HLA-A, -B, and -DR. RESULTS: We present the analysis of HLA frequencies and haplotypes estimates for the subset of our donor database that is additionally typed for HLA-C. We analyzed HLA data from 2657 donors. Antigen and haplotype frequencies were estimated through the use of expectation maximization. CONCLUSIONS: Our analysis showed for the first time the antigenic HLA frequency distribution from HPC donors in Argentina. Knowing haplotype frequencies in our population will help us to select potential donors for high-resolution typing for the patients.
Assuntos
Transplante de Células-Tronco Hematopoéticas , Doadores de Tecidos , Adulto , Argentina , Feminino , Frequência do Gene , Antígenos HLA-A/genética , Antígenos HLA-B/genética , Antígenos HLA-C , Haplótipos , Teste de Histocompatibilidade , Humanos , Masculino , Sistema de RegistrosRESUMO
OBJECTIVES: Recent work has shown that human bone marrow contains mesenchymal stem cells (MSCs). However, little is known about their presence in peripheral blood. Since these cells are potentially responsible for tissue repair after injury, their number should be increased during these situations. To demonstrate their number during these situations, we measured MSCs in the peripheral blood of healthy donors and burn patients. MATERIALS AND METHODS: Blood samples were obtained from 15 acute burn patients and 15 healthy donors. We performed flow cytometric analysis, using a large monoclonal antibody panel: CD44, CD45, CD14, DR, CD34, CD19, CD13, CD29, CD105, CD1a, CD90, CD38, CD25. MSC phenotype was considered positive for CD44, CD13, CD29, CD90, and CD105, and negative for the other monoclonals. The testing was performed on day 3 after injury. We correlated the results with the age, sex, and size and type of burns. RESULTS: Cells expressing the MSC phenotype were detected in the peripheral blood of both groups. Noteworthy, compared with samples from healthy donors (0.0078 +/- 0.0044), blood obtained from burn patients showed a higher MSC percentage (0.1643 +/- 0.115; P < .001). The percentage of MSCs correlated with the size and severity of the burn. Increased values were also observed among younger patients. CONCLUSIONS: MSCs have an important role in regenerative processes of human tissues. We found cells phenotypically identical to MSCs circulating in physiological number in normal subjects, but in significantly higher amounts during acute large burns. Therefore, they may represent a previously unrecognized circulatory component to the process of skin regeneration.
Assuntos
Células da Medula Óssea/fisiologia , Queimaduras/fisiopatologia , Mesoderma/fisiologia , Células-Tronco/fisiologia , Cicatrização , Adulto , Antígenos CD/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , RegeneraçãoRESUMO
INTRODUCTION: We sought to use human mesenchymal stem cells (HMSC) for skin and spinal cord repair in mice. MATERIALS AND METHODS: Human bone marrow obtained from a young healthy donor was used to separate and culture human mesenchymal stem cells (HMSC). Ten mice were included in each of four groups. A full-thickness skin defect was surgically performed on all mice in groups 1 and 2. A transverse complete medullar section was performed in groups 3 and 4. Groups 1 and 3 received HMSC IV infusion and local HMSC polymer implant. Groups 2 and 4 received only the IV HMSC infusion. Five control animals from each group went through the same lesions but they didn't receive treatment. RESULTS: After local administration of HMSC into the fibrin polymer combined with the IV infusion of HMSC, there was no immune rejection; all skin defects healed without scar or retraction at a median time of 14 days. Sixty percent of the animals treated with IV infusion and polymer with HMSC simultaneously had improved neurological activities, while all control mice with spinal cord injury experiments died or perpetuated their paralysis with worsening muscular atrophy and increasing propensity to skin damage. CONCLUSIONS: HMSC are not immunologically reactive and can trespass species defense barriers. Animals treated with these cells repaired injuries better than controls. In this way we propose that universal HMSC from donors can be cultured, expanded, and cryopreserved to be used in human organ or tissue regeneration.
Assuntos
Mesoderma/citologia , Pele/lesões , Traumatismos da Medula Espinal/terapia , Transplante de Células-Tronco , Células-Tronco/citologia , Animais , Técnicas de Cultura de Células/métodos , Humanos , Camundongos , Transplante HeterólogoAssuntos
Doença Enxerto-Hospedeiro/imunologia , Transplante de Células-Tronco Hematopoéticas , Linfócitos T/imunologia , Doença Aguda , Adulto , Feminino , Doença Enxerto-Hospedeiro/diagnóstico , Humanos , Imunidade Celular , Leucemia Mielogênica Crônica BCR-ABL Positiva/imunologia , Leucemia Mielogênica Crônica BCR-ABL Positiva/terapia , Leucemia Mieloide/imunologia , Leucemia Mieloide/terapia , Linfoma/imunologia , Linfoma/terapia , Linfoma não Hodgkin/imunologia , Linfoma não Hodgkin/terapia , Masculino , Pessoa de Meia-Idade , Mieloma Múltiplo/imunologia , Mieloma Múltiplo/terapia , Estudos Prospectivos , Transplante AutólogoAssuntos
Antígenos CD/sangue , Transplante de Medula Óssea/imunologia , Doença Enxerto-Hospedeiro/diagnóstico , Doença Enxerto-Hospedeiro/imunologia , Subpopulações de Linfócitos/imunologia , Anemia Aplástica/terapia , Subpopulações de Linfócitos B/imunologia , Citometria de Fluxo/métodos , Humanos , Imunofenotipagem/métodos , Leucemia/terapia , Doadores Vivos , Núcleo Familiar , Valor Preditivo dos Testes , Subpopulações de Linfócitos T/imunologia , Transplante HomólogoAssuntos
Anemia Aplástica/terapia , Transplante de Medula Óssea/imunologia , Transplante de Células-Tronco Hematopoéticas , Leucemia/terapia , Linfoma não Hodgkin/terapia , Mieloma Múltiplo/terapia , Linfócitos T/imunologia , Adulto , Anemia Aplástica/imunologia , Ciclosporina/uso terapêutico , Doença Enxerto-Hospedeiro/prevenção & controle , Humanos , Imunofenotipagem , Imunossupressores/uso terapêutico , Leucemia/imunologia , Linfoma não Hodgkin/imunologia , Metotrexato/uso terapêutico , Pessoa de Meia-Idade , Mieloma Múltiplo/imunologia , Transplante Autólogo , Transplante HomólogoRESUMO
Se estudiaron antígenos de histocompatibilidad, sistema HLA-loci ABC y DR en una muestra de 30 pacientes alcohólicos. El criterio de inclusión de los pacientes fue muy riguroso, y referido a la presencia de signos y síntomas del síndrome de abstinencia. Los valores encontrados se compararon con los determinados en la población general. Se encontró un aumento de los antígenos B-40 y DR4, y una disminución del DR3 en la población alcohólica. Se determinó riesgo relativo, fracción etiológica y fracción preventiva para desarrollar el síndrome de abstinencia en sujetos alcohólicos que presentaban los citados antígenos
Assuntos
Adulto , Pessoa de Meia-Idade , Humanos , Alcoolismo/genética , Antígenos HLA/genética , Frequência do Gene , Antígenos de Grupos Sanguíneos/genética , Fígado/patologiaRESUMO
Se estudiaron antígenos de histocompatibilidad, sistema HLA-loci ABC y DR en una muestra de 30 pacientes alcohólicos. El criterio de inclusión de los pacientes fue muy riguroso, y referido a la presencia de signos y síntomas del síndrome de abstinencia. Los valores encontrados se compararon con los determinados en la población general. Se encontró un aumento de los antígenos B-40 y DR4, y una disminución del DR3 en la población alcohólica. Se determinó riesgo relativo, fracción etiológica y fracción preventiva para desarrollar el síndrome de abstinencia en sujetos alcohólicos que presentaban los citados antígenos (AU)