RESUMO
This study evaluated the fumigant ethanedinitrile (EDN) against the cigarette beetle, Lasioderma serricorne, and phosphine-resistant and susceptible lesser grain borer, Rhyzopertha dominica, life stages under laboratory conditions. Eggs of both species were the most susceptible stage to EDN. EDN is, therefore, a promising alternative because eggs are generally tolerant to most common fumigants. Lasioderma serricorne eggs were the most susceptible with an LC50 estimated of 50.4 ppm, followed by adults, pupae and larvae with LC50 values of 160.2, 192.5, and 446.6 ppm, respectively, after 24-h exposure at 25°C. Eggs of phosphine-susceptible (LC50 = 11.2 ppm) and resistant (LC50 = 12.0 ppm) R. dominica strains were more susceptible to EDN than were adults of both strains, with LC50 values of 27.7 and 36.0 ppm, respectively. Lasioderma serricorne mixed life stage cultures were completely controlled at concentrations ≥2,000 ppm at 24 h. Fumigation with 600 ppm was enough to suppress adult emergence in the case of the phosphine-susceptible R. dominica strain (USDA), while an average of only 4.0 adults emerged from the phosphine-resistant R. dominica strain (Belle Glade) compared with 514.3 adults in the control. Lasioderma serricorne was more tolerant to EDN than both R. dominica strains. EDN caused 61.8 and 68.2 % inhibition of R. dominica (USDA) cytochrome c oxidase activity at concentrations of 0.0038 and 0.0076 mM in vitro, respectively, and it did not inhibit its activity in the case of an in vivo assay. These results suggest that cytochrome c oxidase may not be the main target for EDN toxicity.
Assuntos
Besouros , Inseticidas , Animais , Dominica , Fumigação , Inseticidas/toxicidade , NitrilasRESUMO
BACKGROUND: Rhyzopertha dominica is a serious pest of stored grains and many populations have resistance to the fumigant phosphine. Some populations contain beetles with a 'strong resistance' phenotype. Recent work found the LC50 values for two strong-resistant populations recently studied in North America, Belle Glade and Minneapolis were 100- and 595-fold higher, respectively, compared to LC50 of a lab-susceptible strain. Populations with 'weak-resistant' phenotypes had LC50 values 5- to 10-fold that of a susceptible strain. The work reported below aimed to determine the minimum phosphine concentrations and number of days of exposure needed to effectively control all life stages of representative weak- and strong-resistant strains, and then to recommend the treatment conditions needed to control strongly phosphine-resistant R. dominica in pest populations. RESULTS: A dose-mortality assay estimated that phosphine fumigation over 48 h using 730-870 ppm at 25° C would control adults of both strongly resistant R. dominica populations. Fumigations with mixed life stage cultures found 200 ppm killed all susceptible and weak-resistant beetles in 2 days, but the strong-resistant Minneapolis and Belle Glade strains had substantial survivors at 200 ppm. Furthermore, the Belle Glade strain had beetles that survived 1000 ppm in 2-day fumigations. The strong-resistant Belle Glade strain needed nearly 10 days at over 400 ppm to have acceptable levels of control. CONCLUSION: This study recommends protocols to manage strongly resistant R. dominica populations provided that a minimum phosphine concentration of 400 ppm be maintained at 25° C or higher for up to 10 days. © 2019 Society of Chemical Industry.
Assuntos
Besouros , Animais , Dominica , Resistência a Inseticidas , Inseticidas , América do Norte , FosfinasRESUMO
Phosphine is the most widely used fumigant for stored grain insect pests, and resistance to phosphine has evolved in several species worldwide. This study was designed to determine the presence of phosphine resistance in 34 populations of Rhyzopertha dominica (F.) collected from the United States and Canada. Adult R. dominica were sampled and subjected to a discriminatory dose toxicity assay of exposure to 20 ppm of phosphine for 20 h of exposure to distinguish a susceptible R. dominica adult by death from a resistant beetle that survives the treatment. All but two of the 34 geographic populations surveyed had some beetles that were resistant to phosphine, and the frequency of resistance varied from 97% in a population from Parlier, California to 0% in beetles from both Carnduff, Saskatchewan and Starbuck, Manitoba. Probit analyses of dose-mortality bioassays with beetles from a laboratory-susceptible strain and those from five of the populations sampled were used to calculate resistance ratio factors (RRs) based on the ratio of LC50 (estimate for the concentration to kill 50% of a test group) in the sampled population to the LC50 for the susceptible strain. The highest RR for the five resistant populations was nearly 596-fold in beetles from Belle Glade, Florida, whereas the lowest RR in that group was 9-fold in Wamego, Kansas. This study revealed that phosphine resistance in R. dominica is common across North America and some populations have levels of resistance that may pose challenges for continued use of phosphine for their management.
Assuntos
Besouros/efeitos dos fármacos , Resistência a Inseticidas , Inseticidas/farmacologia , Fosfinas/farmacologia , Animais , Canadá , Besouros/crescimento & desenvolvimento , Besouros/fisiologia , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Larva/fisiologia , Estados UnidosRESUMO
Grain stored in bins is initially a relatively homogenous resource patch for stored-product insects, but over time, spatial pattern in insect distribution can form, due in part to insect movement patterns. However, the factors that influence stored-product insect movement patterns in grain are not well-understood. This research focused on the movement of the lesser grain borer, Rhyzopertha dominica (F.), within a simulated wheat grain mass (vertical monolayer of wheat) and the identification of factors that contribute to overall and upward movement (age since adult emergence from an infested kernel [1, 7, and 14 d], sex, strain, and different levels of environment quality). We also used the model selection approach to select the most relevant factors and determine the relationships among them. Three-week-old adults tended to stay closer to the surface compared with 1- or 2-wk-old insects. Also, females tended to be more active and to explore a larger area compared with males. Explored area and daily displacement were also significantly strain-dependent, and increasing grain infestation level decreased daily displacement and explored area. Variation in movement pattern is likely to influence the formation of spatial pattern and affect probability to disperse. Understanding movement behavior within a grain bin is crucial to designing better strategies to implement and interpret monitoring programs and to target control tactics.
Assuntos
Besouros/fisiologia , Herbivoria , Animais , Tomada de Decisões , Grão Comestível , Comportamento Alimentar , Feminino , Longevidade , Masculino , Movimento , Orientação , TriticumRESUMO
Stored product beetles that are resistant to the fumigant pesticide phosphine (hydrogen phosphide) gas have been reported for more than 40 years in many places worldwide. Traditionally, determination of phosphine resistance in stored product beetles is based on a discriminating dose bioassay that can take up to two weeks to evaluate. We developed a diagnostic cleaved amplified polymorphic sequence method, CAPS, to detect individuals with alleles for strong resistance to phosphine in populations of the red flour beetle, Tribolium castaneum, and the lesser grain borer, Rhyzopertha dominica, according to a single nucleotide mutation in the dihydrolipoamide dehydrogenase (DLD) gene. We initially isolated and sequenced the DLD genes from susceptible and strongly resistant populations of both species. The corresponding amino acid sequences were then deduced. A single amino acid mutation in DLD in populations of T. castaneum and R. dominica with strong resistance was identified as P45S in T. castaneum and P49S in R. dominica, both collected from northern Oklahoma, USA. PCR products containing these mutations were digested by the restriction enzymes MboI and BstNI, which revealed presence or absence, respectively of the resistant (R) allele and allowed inference of genotypes with that allele. Seven populations of T. castaneum from Kansas were subjected to discriminating dose bioassays for the weak and strong resistance phenotypes. Application of CAPS to these seven populations confirmed the R allele was in high frequency in the strongly resistant populations, and was absent or at a lower frequency in populations with weak resistance, which suggests that these populations with a low frequency of the R allele have the potential for selection of the strong resistance phenotype. CAPS markers for strong phosphine resistance will help to detect and confirm resistant beetles and can facilitate resistance management actions against a given pest population.
Assuntos
Besouros/efeitos dos fármacos , Marcadores Genéticos , Resistência a Inseticidas/genética , Fosfinas/farmacologia , Tribolium/efeitos dos fármacos , Alelos , Sequência de Aminoácidos , Animais , Besouros/genética , DNA Complementar , Genótipo , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Tribolium/genética , Estados UnidosRESUMO
The lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), a serious pest of stored cereal grains, is widely distributed and has been collected in different habitats in North America, such as those from agricultural and nonagricultural settings. Our objective was to study the dispersal distances and direction of dispersal by R. dominica after external marking using fluorescent powder, releasing marked beetles, and recapturing adults using pheromone traps in distinctively different ecological habitats, wooded sites and open grasslands, for 2 consecutive yr. The recapture rate of marked beetles ranged from 6 to 26% in both sites and was generally higher in the wooded site than the open field site for both years. There was a significant difference in dispersal distances between wooded and open sites. Mean dispersal distances in the wooded site ranged from 337 to 375 m, whereas in the open site, they varied from 261 to 333 m. Trap captures for both marked and feral beetles were related to the ambient temperature such that increase in trap captures occurred with increasing temperature. Significant differences were observed for directional movement of R. dominica in both sites and indicated that most beetles dispersed in the northwest direction. Correlation analyses showed that the relationship between numbers of marked-released-recaptured beetles significantly decreased with increasing trap distances. Understanding dispersal distances and directions provide insight to flight behavior of R. dominica and to the relationship between ecologically diverse breeding habitats. Knowledge of R. dominica habitat ecology outside of grain storage facilities may be useful in designing suitable management tactics to minimize the onset of infestations in grain storages.