RESUMO
Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.
Assuntos
Biofilmes/efeitos dos fármacos , Quelantes/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Animais , Cálcio/análise , Bovinos , Quelantes/química , Cavidade Pulpar/química , Ácido Edético/química , Ácido Edético/farmacologia , Enterococcus faecalis/química , Concentração de Íons de Hidrogênio , Íons , Ferro/análise , Manganês/análise , Teste de Materiais , Irrigantes do Canal Radicular/química , Irrigantes do Canal Radicular/farmacologia , Hipoclorito de Sódio/química , Hipoclorito de Sódio/farmacologia , Espectrofotometria Atômica , Água/química , Zinco/análiseRESUMO
Abstract Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.
Assuntos
Animais , Bovinos , Quelantes/farmacologia , Enterococcus faecalis/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/microbiologia , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/química , Hipoclorito de Sódio/farmacologia , Hipoclorito de Sódio/química , Espectrofotometria Atômica , Teste de Materiais , Água/química , Quelantes/química , Cálcio/análise , Ácido Edético/farmacologia , Ácido Edético/química , Enterococcus faecalis/química , Cavidade Pulpar/química , Concentração de Íons de Hidrogênio , Íons , Ferro/análise , Manganês/análiseRESUMO
OBJECTIVES: The aim of the present study was to evaluate cytotoxic effects and cytokine production of calcium silicate-based sealers (EndoSeal, EndoSequence BC Sealer, and MTA Fillapex) using an in vitro root canal filling model and three-dimensional (3D) cell culture. AH Plus as a reference was compared to contemporary calcium silicate cements regarding cell viability and cytokine production. MATERIAL AND METHODS: Root canals of 30 human maxillary incisors were prepared using a single-file reciprocating technique. The samples were randomly distributed and canals filled with either AH Plus, EndoSeal, EndoSequence BC Sealer, and MTA Fillapex (n = 6). In the negative control group, the root canal remained unfilled. Sealers were placed into the canals along with a gutta-percha cone placed to working length. Balb/c 3T3 fibroblasts, cultured in a type I collagen 3D scaffold, were exposed to filling material and the respective root apex for 24 h. Cytocompatibility of the materials was evaluated using the methyl-thiazoldiphenyl-tetrazolium (MTT) assay. The production of IL-1ß, IL-6, and IL-8 was analyzed using enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance was performed, and when the F-ratios were significant, data were compared by Duncan's multiple-range test. The alpha-type error was set at 0.05. RESULTS: EndoSeal, Endosequence BC Sealer and AH Plus showed cell viability that was similar to the negative control group (P > 0.05), while MTA Fillapex sealer was cytotoxic (P < 0.05). Varying production of IL-1ß, IL-6, and IL-8 was detected in all samples. CONCLUSIONS: In an in vitro root canal filling model with 3D cell culture, AH Plus, EndoSeal, and EndoSequence BC Sealer were cytocompatible. CLINICAL RELEVANCE: These results may suggest that AH Plus, EndoSeal and EndoSequence BC Sealer may achieve better biological response when compared to MTA Fillapex.
Assuntos
Materiais Biocompatíveis/farmacologia , Compostos de Cálcio/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos/farmacologia , Compostos de Alumínio/farmacologia , Compostos de Alumínio/toxicidade , Materiais Biocompatíveis/toxicidade , Compostos de Cálcio/toxicidade , Fosfatos de Cálcio , Técnicas de Cultura de Células , Células Cultivadas , Citocinas/metabolismo , Combinação de Medicamentos , Resinas Epóxi , Guta-Percha/farmacologia , Guta-Percha/toxicidade , Humanos , Técnicas In Vitro , Incisivo , Teste de Materiais , Óxidos/farmacologia , Óxidos/toxicidade , Materiais Restauradores do Canal Radicular/toxicidade , Silicatos/toxicidade , Propriedades de SuperfícieRESUMO
INTRODUCTION: The purpose of this study was to evaluate the effect of 2 different temperatures (20°C and 37°C) on the cyclic fatigue life of rotary instruments and correlate the results with martensitic transformation temperatures. METHODS: Contemporary nickel-titanium rotary instruments (n = 20 each and tip size #25, including Hyflex CM [Coltene, Cuyahoga Falls, OH], TRUShape [Dentsply Tulsa Dental Specialties, Tulsa, OK], Vortex Blue [Dentsply Tulsa Dental Specialties], and ProTaper Universal [Dentsply Tulsa Dental Specialties]) were tested for cyclic fatigue at room temperature (20°C ± 1°C) and at body temperature (37°C ± 1°C). Instruments were rotated until fracture occurred in a simulated canal with an angle curvature of about 60° and a radius curvature of 3 mm; the center of the curvature was 4.5 mm from the instrument tip. The number of cycles to fracture was measured. Phase transformation temperatures for 2 instruments of each brand were analyzed by differential scanning calorimetry. Data were analyzed using the t test and 1-way analysis of variance with the significance level set at 0.05. RESULTS: For the tested size and at 20°C, Hyflex CM showed the highest resistance to fracture; no significant difference was found between TRUShape and Vortex Blue, whereas ProTaper Universal showed the lowest resistance to fracture. At 37°C, resistance to fatigue fracture was significantly reduced, up to 85%, for the tested instruments (P < .001); at that temperature, Hyflex CM and Vortex Blue had similar and higher fatigue resistance compared with TRUShape and ProTaper Universal. CONCLUSIONS: Under the conditions of this study, using a novel testing design, immersion in water at simulated body temperature was associated with a marked decrease in the fatigue life of all rotary instruments tested.
Assuntos
Temperatura Corporal , Desenho de Equipamento , Teste de Materiais , Preparo de Canal Radicular/instrumentação , Rotação , Análise de Variância , Varredura Diferencial de Calorimetria , Ligas Dentárias/química , Falha de Equipamento , Humanos , Níquel/química , Estresse Mecânico , Propriedades de Superfície , Temperatura , Titânio/química , Torque , Torção Mecânica , Temperatura de TransiçãoRESUMO
INTRODUCTION: Periapical inflammation is often responsible for distinct maxillary sinus (MS) changes. This retrospective, cross-sectional study evaluated the association between the clinical characteristics of periapical lesions (presence, size, and distance) in maxillary posterior teeth and the presence of sinus abnormalities by evaluating cone-beam computed tomographic (CBCT) images obtained from an archived collection. Apart from sex, no other patient information was available. METHODS: The study sample was composed of CBCT images of 143 MSs of patients with at least 1 maxillary posterior tooth with a periapical lesion and 178 MSs of patients without periapical radiolucent lesions. Sinus abnormalities were classified as mucosal thickening, sinus polyp, antral pseudocyst, nonspecific opacification, periostitis, and antral calcification; periapical radiolucent areas were classified using the CBCT periapical index, and the distance between the periapical lesion edge and the MS floor was measured. Data were analyzed using chi-square tests at a level of significance set at α = 0.05. RESULTS: Most sinus abnormalities were associated with at least 1 maxillary posterior tooth with a periapical lesion (P > .05). The most frequent sinus abnormality in the presence of a periapical lesion was mucosal thickening. All teeth with a CBCT periapical index score of 5 were associated with sinus abnormalities. The highest frequency of abnormalities was found when the radiolucent area was subjacent to the sinus floor. CONCLUSIONS: Maxillary posterior teeth with periapical radiolucent lesions had the highest frequency of sinus abnormalities. The size of a periapical lesion was not associated with the frequency of sinus abnormalities. A close spatial relationship between periapical lesions and sinuses resulted most frequently in sinus abnormalities.
Assuntos
Seio Maxilar/diagnóstico por imagem , Seio Maxilar/patologia , Periodontite Periapical/diagnóstico por imagem , Periodontite Periapical/patologia , Adulto , Tomografia Computadorizada de Feixe Cônico , Estudos Transversais , Feminino , Humanos , Masculino , Sinusite Maxilar/diagnóstico por imagem , Sinusite Maxilar/patologia , Estudos RetrospectivosRESUMO
OBJECTIVE: Comparison of physical and mechanical properties of one conventional and a new NiTi wire, which had received an additional thermomechanical treatment. METHODS: Specimens of both conventional (NiTi) and the new type of wire, called M-Wire (MW), were subjected to tensile and three-point bending tests, Vickers microhardness measurements, and to rotating-bending fatigue tests at a strain-controlled level of 6%. Fracture surfaces were observed by scanning electron microscopy and the non-deformed microstructures by transmission electron microscopy. RESULTS: The thermomechanical treatment applied to produce the M-Wire apparently increased the tensile strength and Vickers microhardness of the material, but its apparent Young modulus was smaller than that of conventionally treated NiTi. The three-point bending tests showed a higher flexibility for MW which also exhibited a significantly higher number of cycles to failure. SIGNIFICANCE: M-Wire presented mechanical properties that can render endodontic instruments more flexible and fatigue resistant than those made with conventionally processed NiTi wires.
Assuntos
Endodontia/instrumentação , Teste de Materiais , Níquel , Titânio , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Resistência à TraçãoRESUMO
INTRODUCTION: This in vitro study aimed to compare the antibacterial effect of a new irrigant, QMiX, with that of conventional irrigation solutions in the presence or absence of dentin powder. METHODS: Dentin powder was prepared from bovine incisors and sterilized. The following irrigants were tested against Enterococcus faecalis (ATCC 29212): 6% sodium hypochlorite (NaOCl), 1% NaOCl, QMiX, 2% chlorhexidine gluconate (CHX), and 17% EDTA. Sterilized saline solution was used as negative control. Survival of bacteria exposed to the irrigants in the presence or absence of dentin was monitored under planktonic conditions. Colony-forming units were counted, and log-transformed numbers were analyzed by using Kruskal-Wallis and Mann-Whitney tests. P values less than .05 were considered significant. RESULTS: In the absence of dentin, after 10 seconds of contact with the bacterial suspension, 6% NaOCl showed the lowest bacterial count; the difference to the negative control was significant. After 30 seconds, 6% NaOCl displayed 0 colony-forming units per milliliter, whereas 1% NaOCl and QMiX showed reduced number of colonies in comparison with the negative control. After 1 minute, both concentrations of NaOCl presented no bacterial growth and QMiX reduced the number of colonies, but EDTA and CHX had bacterial counts similar to the negative control. Dentin had a significant inhibitory effect on 6% NaOCl (10 seconds), 1% NaOCl (10 seconds and 1 minute), and QMiX (10 seconds and 1 minute). After 6 hours, both concentrations of NaOCl, QMiX, and CHX killed all bacteria, regardless of the presence of dentin. CONCLUSIONS: Six percent NaOCl was the most effective irrigant against E. faecalis. Saline and EDTA had no measurable antibacterial effect. Dentin delayed the antibacterial activity of NaOCl and QMiX but did not completely prevent their action.