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This study investigated whether the origin of sperm (epididymal vs. ejaculate) affects the cryopreservation efficiency in agouti (Dasyprocta leporina). Five sexually mature agoutis underwent electroejaculation, resulting in obtaining four semen samples. After 15 days, the same animals were euthanized, and through retrograde flushing, sperm samples were obtained from the epididymis tails. In both collection methods, samples were evaluated for sperm parameters (sperm concentration, motility, vigor, membrane integrity, osmotic response, and morphology). Then, samples were diluted in ACP 109c, added with 20% egg yolk, and a final concentration of 6% glycerol. Finally, the samples were packaged in 0.25 mL straws and frozen in liquid nitrogen. After one week, samples were thawed and evaluated in the same way as fresh samples, with the addition of membrane integrity analysis using fluorescent probes (C-FDA/PI) and computerized analysis (CASA). Immediately after obtaining the sperm, samples obtained directly from the epididymis presented higher values (P ≤ 0.05) than those obtained by electroejaculation concerning the parameters of volume, sperm concentration, and total number of sperm (1,398.25 ± 206.0 x106 and 184.5 ± 78.0 x106 sperm). On the other hand, in the classical evaluation of the other sperm parameters and the computerized analysis (CASA) after thawing, such as total motility, no statistical differences were observed between sperm from both origins (ejaculate: 16.7 ± 8.2% and epididymal: 24.8 ± 12.0%, P > 0.05). This demonstrates the possibility of direct application of the cryopreservation protocol for agouti (D. leporina) sperm obtained via the epididymis or ejaculate.

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The aim of this study was to evaluate the diagnosis of estrous in Canindé goats used infrared thermographic images. The Canindé goats used in experiment, belong to Production Center of Small Ruminants. The goats were subjected to estrus induction and monitored with regard to their behavior and blood levels of hormones. Temperature measurements were continuous during estrous signs and even disappearance of behavioral characteristics. Were evaluated different areas in females: vulva, perivulvar, dorse and venter regions, delimited by a quadrant that allowed the specification of assessed region. In addition to specific behavior of estrus in ruminants were observed in vulvar region perivulvar signals swelling and redness and parallel identified the temperature rise of region by thermographic images. Were observed in vulvar region perivulvar signals swelling and redness and parallel identified the temperature rise of that region by thermographic images in goats in estrus. There was a significant effect for all temperatures of the regions evaluated for the thermographic images and infrared thermometer, except for the temperature of the vulvar region. The temperatures measured using the thermographic camera submitted a difference in the data set, allowing measurements observed higher temperatures compared with the temperatures measured with an infrared thermometer. So, due the precision and speed of the method the thermographic images used for detecting estrus is applicable and important. In animal production, thermography has seen used in detection of metabolic disorders, diseases and infections. In addiction, is usedin understanding thermoregulation due to chenges in temperature and the impact of environmental conditions.(AU)

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The aim of this study was to evaluate the diagnosis of estrous in Canindé goats used infrared thermographic images. The Canindé goats used in experiment, belong to Production Center of Small Ruminants. The goats were subjected to estrus induction and monitored with regard to their behavior and blood levels of hormones. Temperature measurements were continuous during estrous signs and even disappearance of behavioral characteristics. Were evaluated different areas in females: vulva, perivulvar, dorse and venter regions, delimited by a quadrant that allowed the specification of assessed region. In addition to specific behavior of estrus in ruminants were observed in vulvar region perivulvar signals swelling and redness and parallel identified the temperature rise of region by thermographic images. Were observed in vulvar region perivulvar signals swelling and redness and parallel identified the temperature rise of that region by thermographic images in goats in estrus. There was a significant effect for all temperatures of the regions evaluated for the thermographic images and infrared thermometer, except for the temperature of the vulvar region. The temperatures measured using the thermographic camera submitted a difference in the data set, allowing measurements observed higher temperatures compared with the temperatures measured with an infrared thermometer. So, due the precision and speed of the method the thermographic images used for detecting estrus is applicable and important. In animal production, thermography has seen used in detection of metabolic disorders, diseases and infections. In addiction, is usedin understanding thermoregulation due to chenges in temperature and the impact of environmental conditions.

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Dasyprocta spp. (agouti) include wild rodents with highlighted ecological and economic importance, and are considered experimental models for endangered hystricognath rodents. Of late, development of techniques to conserve their genetic material as well as the formation of biobanks is increasing. In this context, this review describes the main advances in the knowledge of the reproductive morphophysiological specificities of agouti as well as the development and improvement of assisted reproductive techniques aimed at conservation, multiplication, and exploitation of their reproductive potential under captivity.

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Background: Ingestion of foreign bodies is one of the most common cases seen among puppies of dogs in the medical clinics of small animals which may be related to their curious behavior. The diagnosis is based on the patients history, clinical signs, and complementary diagnostic imaging tests. The present case report describes the use of complementary imaging tests, showing a partial gastric obstruction caused by cajarana (Spondias cytherea Sonn) in a puppy.Case: At the Veterinary Hospital of the Federal Rural University of the Semi-Arid Region, a 4-month-old male Yorkshire dog was treated. The guardian reported having witnessed the ingestion of a cajarana stone by the animal. During the physical examination, the animal was active, with good nutritional status, normocorated mucosae, and a moderate degree of dehydration, besides a slight pain on palpation in the epigastric region. Hematological examinations included blood counts, blood urea, creatinine, and alkaline phosphatase. A moderate degree of polycythemia was observed, which may be explained by the dehydration caused by the episodes of vomiting. The patient had frequent episodes of vomiting after the reported event and was then referred to the diagnostic imaging sector. During the sonographic examination, a concave echogenic interface associated with a posterior acoustic shadowing was observed in the region of the stomach. The animal was sent for simple radiographs and positive contrast based on barium sulfate due to the suspicion of the presence of a foreign body. On simple radiography, in the left laterolateral view, a little radiopaque structure of circular format with poor definition was observed in the pyloric region. After the simple radiography, approximately 10 mL of barium sulfate contrast was administered orally followed by a new left lateral radiographic imaging.[...](AU)

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Dasyprocta spp. (agouti) include wild rodents with highlighted ecological and economic importance, and are considered experimental models for endangered hystricognath rodents. Of late, development of techniques to conserve their genetic material as well as the formation of biobanks is increasing. In this context, this review describes the main advances in the knowledge of the reproductive morphophysiological specificities of agouti as well as the development and improvement of assisted reproductive techniques aimed at conservation, multiplication, and exploitation of their reproductive potential under captivity.(AU)

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Background: Ingestion of foreign bodies is one of the most common cases seen among puppies of dogs in the medical clinics of small animals which may be related to their curious behavior. The diagnosis is based on the patient’s history, clinical signs, and complementary diagnostic imaging tests. The present case report describes the use of complementary imaging tests, showing a partial gastric obstruction caused by cajarana (Spondias cytherea Sonn) in a puppy.Case: At the Veterinary Hospital of the Federal Rural University of the Semi-Arid Region, a 4-month-old male Yorkshire dog was treated. The guardian reported having witnessed the ingestion of a cajarana stone by the animal. During the physical examination, the animal was active, with good nutritional status, normocorated mucosae, and a moderate degree of dehydration, besides a slight pain on palpation in the epigastric region. Hematological examinations included blood counts, blood urea, creatinine, and alkaline phosphatase. A moderate degree of polycythemia was observed, which may be explained by the dehydration caused by the episodes of vomiting. The patient had frequent episodes of vomiting after the reported event and was then referred to the diagnostic imaging sector. During the sonographic examination, a concave echogenic interface associated with a posterior acoustic shadowing was observed in the region of the stomach. The animal was sent for simple radiographs and positive contrast based on barium sulfate due to the suspicion of the presence of a foreign body. On simple radiography, in the left laterolateral view, a little radiopaque structure of circular format with poor definition was observed in the pyloric region. After the simple radiography, approximately 10 mL of barium sulfate contrast was administered orally followed by a new left lateral radiographic imaging.[...]

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Dasyprocta spp. (agouti) include wild rodents with highlighted ecological and economic importance, and are considered experimental models for endangered hystricognath rodents. Of late, development of techniques to conserve their genetic material as well as the formation of biobanks is increasing. In this context, this review describes the main advances in the knowledge of the reproductive morphophysiological specificities of agouti as well as the development and improvement of assisted reproductive techniques aimed at conservation, multiplication, and exploitation of their reproductive potential under captivity.

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In collared peccaries, the development of artificial insemination (AI) is scarce, requiring search for alternative methods for the evaluation of sperm fertilizing ability. Thus, the aims of this study were to estimate the binding capability of collared peccaries sperm, using swine oocytes and the egg perivitelline membrane, and to evaluate the prognostic value of sperm parameters on the in vitro interactions among sperm and heterologous substrates. Eleven ejaculates were collected by eletroejaculation and evaluated for viability and morphology by light microscopy, for functionality by hypo-osmotic swelling test, for plasma membrane integrity by epifluorescence microscopy, and for sperm motility by computerized analysis. Subsequently, for analysis of the in vitro interactions, sperm samples were cultured in an incubation medium with swine oocytes and egg perivitelline membrane for 18 h and 20 min, respectively, at 38.5 °C and humidified atmosphere. The sperm-oocyte interaction rate was 100% with sperm penetrating 19.8+ 5.5% of oocytes. The average values of bound sperm and penetrated sperm per oocyte were 39.4 + 4.6 and 2.5 + 0.7, respectively. Already for perivitelline membrane binding assay, all samples presented sperm bound (100%) with average of 140.6 ± 19.4 bound sperm (range 33.9-308.7). Moreover, positive correlations were observed for the number of sperm bound to swine oocytes and osmotic response (r = 68.5%; P = 0.02), membrane integrity (r = 65.1%; P = 0.03), and straightness (r = 66.5%; (P = 0.03), as weel as for the number of sperm bound to egg perivitelline membrane and sperm viability (r = 74.0%; P = 0.01), total motility (r = 63.6%; P = 0.04), and linearity (r = 70.5%; P = 0.02). Finally, a negative correlation among slow (r = -80.5%; P = 0.01) and static (r = -84.3%; P = 0.01) sperm with the egg perivitelline membrane was observed. In conclusion, swine oocytes and perivitelline membrane can be used as indicators for the functional evaluation of the binding capability of sperm derived from collared peccaries. These tests could be incorporated into the routine of semen technologies.

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The aim of the current study was to compare sperm quality characteristics of the collared peccary (Pecari tajacu) following freezing in extenders supplemented with whole egg yolk and different concentrations of low-density lipoproteins (LDL). Semen from 11 adult males was obtained by electroejaculation and evaluated for sperm motility, vigor, morphology as well as membrane integrity analyzed by the hypo-osmotic swelling (HOS) test and a fluorescent staining. Moreover, the semen was diluted in a Tris-based extender containing 20% egg yolk (control group) or 5, 10 or 20% LDL (treatment groups). The semen samples were frozen in liquid nitrogen and thawed in a water bath for 60s at 37°C. The treatments did not affect (p>0.05) sperm vigor, morphology or membrane integrity analyzed by the HOS test. However, post-thaw sperm motility was significantly higher (p<0.05) in the extender supplemented with 20% LDL (36.4 ± 5.3%) compared with the egg yolk extender and extender supplemented with 10% LDL. Furthermore, the percentage of membrane-intact frozen-thawed spermatozoa analyzed by the fluorescent staining was significantly higher (p<0.05) in the extender supplemented with 20% LDL (27.4 ± 6.5%) than in the other groups. In conclusion, 20% LDL can be used to substitute the whole egg yolk as a cryoprotective additive for freezing semen of the collared peccary.

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We verify the efficiency of a protocol for estrus synchronization in captive female collared peccaries (Pecaricari tajacu) using the prostaglandin analog D-cloprostenol. Five adult female collared peccaries received an intramuscular administration of 60 µg D-cloprostenol, which procedure was repeated after a 9-day interval. For 10 days after second the D-cloprostenol administration, females were monitored for changes in external genitalia, ovarian ultrasonography, vaginal cytology and reproductive hormonal dosage. As a result, four females synchronized their estrous at 9.5 ± 0.5 days after the second administration of the prostaglandin analog. Such females showed external signs of estrus, including vulvar opening, hyperemic vaginal mucosa, and vaginal mucus, concomitant with an increase in the proportion of superficial cells (52.2 ± 9.9%) verified through vaginal cytology. An estrogen peak of 22.7 ± 3.4 pg/ml was detected by hormonal dosage, and the presence of anechoic follicles measuring 0.29 ± 0.05 × 0.32 ± 0.07 mm were detected in the ovary by ultrasonography. Given these findings, we suggest that D-cloprostenol may be effective for use in estrus synchronization in collared peccaries.

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Background: The development of semen cryopreservation protocols for collared peccaries (Pecari tajacu) would contribute to its preservation and multiplication. Nowadays, studies on this subject report the use of Tris and powdered coconutwater (ACP®-116c) as extenders. However, such studies are limited to the post-thawing immediate evaluation of samples.The knowledge on the sperm survival after thawing would be important in order to schedule the moment and the vial forartificial insemination. Therefore, the aim of the present research was to verify the effect of Tris and ACP extenders on thesperm survival of frozen-thawed semen, centrifuged or not, derived from collared peccaries.Materials, Methods & Results: Semen from 12 adult males collared peccaries were obtained by electroejaculation. Ejaculateswere immediately evaluated for sperm motility, vigor, viability, functional membrane integrity and morphology. Sampleswere diluted in Tris or ACP, both plus 10% egg yolk and 3% glycerol. Then, they were packaged into 0.25 mL straws, andstored in liquid nitrogen. After one week, samples were thawed at 38ºC/1 min and divided into two aliquots: one immediately diluted in the same extender, and the other submitted to centrifugation and then re-diluted. A thermorresistance testwas conducted including assessments at 0, 15, 30, 45, and 60 min as described for fresh semen. After analyzing the dataimmediately after thawing, we verify that coconut water provided a better preservation of sperm motility (41.3 ± 6.9%)and vigor (2.9 ± 0.2) than Tris, 33.1 ± 5.9% motile sperm with vigor 2.5 ± 0.2 (P < 0.05). During thermorresistance test...

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Background: The development of semen cryopreservation protocols for collared peccaries (Pecari tajacu) would contribute to its preservation and multiplication. Nowadays, studies on this subject report the use of Tris and powdered coconutwater (ACP®-116c) as extenders. However, such studies are limited to the post-thawing immediate evaluation of samples.The knowledge on the sperm survival after thawing would be important in order to schedule the moment and the vial forartificial insemination. Therefore, the aim of the present research was to verify the effect of Tris and ACP extenders on thesperm survival of frozen-thawed semen, centrifuged or not, derived from collared peccaries.Materials, Methods & Results: Semen from 12 adult males collared peccaries were obtained by electroejaculation. Ejaculateswere immediately evaluated for sperm motility, vigor, viability, functional membrane integrity and morphology. Sampleswere diluted in Tris or ACP, both plus 10% egg yolk and 3% glycerol. Then, they were packaged into 0.25 mL straws, andstored in liquid nitrogen. After one week, samples were thawed at 38ºC/1 min and divided into two aliquots: one immediately diluted in the same extender, and the other submitted to centrifugation and then re-diluted. A thermorresistance testwas conducted including assessments at 0, 15, 30, 45, and 60 min as described for fresh semen. After analyzing the dataimmediately after thawing, we verify that coconut water provided a better preservation of sperm motility (41.3 ± 6.9%)and vigor (2.9 ± 0.2) than Tris, 33.1 ± 5.9% motile sperm with vigor 2.5 ± 0.2 (P < 0.05). During thermorresistance test...(AU)

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Em ejaculados provenientes de 28 catetos, verificou-se a existência de relações entre a concentração espermática determinada por meio da câmara de Neubauer e a tramitância observada por espectrofotometria, utilizando comprimentos de onda variando de 530 a 590nm. Os ejaculados apresentaram uma concentração média de 283,9±30,8x106 espermatozoides mL-1, com variação de 30 a 640x106 espermatozoides mL-1. Os valores para tramitância variaram entre 36,9 a 96,3, nos diferentes comprimentos de onda. Não foram detectadas relações significativas entre os dois métodos (P>0,05). Dessa forma, não se recomenda a espectrofotometria para os procedimentos de rotina na determinação da concentração espermática em catetos.

In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of 283.9±30.8x106sperm m-1, varying from 30 to 640x106sperm mL-1. Values for tramitance varied from 36.9 to 96.3, under different wavelengths. No significant relationship was verified between two methods (P>0.05). Thus, the spectrophotometer is not recommended for routine procedures of sperm concentration measurement in collared peccaries.

RESUMO

Em ejaculados provenientes de 28 catetos, verificou-se a existência de relações entre a concentração espermática determinada por meio da câmara de Neubauer e a tramitância observada por espectrofotometria, utilizando comprimentos de onda variando de 530 a 590nm. Os ejaculados apresentaram uma concentração média de 283,9±30,8x106 espermatozoides mL-1, com variação de 30 a 640x106 espermatozoides mL-1. Os valores para tramitância variaram entre 36,9 a 96,3, nos diferentes comprimentos de onda. Não foram detectadas relações significativas entre os dois métodos (P>0,05). Dessa forma, não se recomenda a espectrofotometria para os procedimentos de rotina na determinação da concentração espermática em catetos.(AU)

In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of 283.9±30.8x106sperm m-1, varying from 30 to 640x106sperm mL-1. Values for tramitance varied from 36.9 to 96.3, under different wavelengths. No significant relationship was verified between two methods (P>0.05). Thus, the spectrophotometer is not recommended for routine procedures of sperm concentration measurement in collared peccaries.(AU)

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In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P < 0.05). Centrifugation promoted an increase in head and tail defects, thus reducing the percentage of viable sperm (P < 0.05). No other parameter assessed for both methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.

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Background: Transmissible venereal tumor (TVT) is a neoplasm which primarily affects the genital mucosa of canines. However, this tumor has been described in several extragenital areas. Although there are no major difficulties to confirm a TVT when present in the genitalia, it can be confused with several conditions, either neoplastic or non-neoplastic, when located beyond the external reproductive organs. The involvement of multiple extragenital locations is poorly reported, which may compromise the tissues involved and hinder the establishment of a diagnostic conclusion. The study aimed to describe a case of multiple and extragenital TVT in dogs. Case: A two year old mongrel dog had a history of swollen nose and ocular abnormalities. The patient underwent physical examination followed by request of nose and eye cytology. The animal was euthanized and sent for autopsy; the material was intended for cytological diagnosis. It was found an increase in volume in the middle third of the nasal region. In the oral cavity, there was a tumor in the caudal third of the hard palate. Neoformations were also detected in the third eyelid. The nasal and eyelid cytological examination was compatible with TVT plasmacytoid pattern. During the necropsy, it was also detected tumors in the nasal cavity, occupying the space of turbinal bones Proliferation occurred even in the palatine bone and pharynx. In the visual system, the tumors did not involve other attachments or eyeball chambers There were no anatomical lesions In other areas. The cytopathology of all tumors presented diagnosis similar to the initial microscopic findings, thus characterizing a framework of multiple extragenital plasmacytoid TVT. Discussion: A study found that the nasal cavity and skin tissue were the locations most affected by the presentation of extragenital TVT. So, there was a similarity to the present case in relation to the shape of the nasal TVT; however, the occurrence in other sites such as the oral cavity and third eyelid is uncommon, although found in the patient reported. The extragenital location may appear regardless to genital lesions. In the examined animal, the placement of the TVT to extragenital areas such as nasal and ocular adnexa was justified by the fact that these regions correspond to anatomical sites exposed to direct physical contact for the acquisition of neoplastic cells, possibly because of the pre­mating behavior of the male toward the bitch that housed the tumor. As an intranasal neoplasia progresses, some signs, such as the swelling of the hard palate, are presented. Thus, in the current study, the proliferation showed in the oral cavity and the pharynx was regarded as an extension of local mechanical nasal cancer. Recent surveys have ranked the TVT as standard plasmocitoyd, linphocitoyd or mixed. It has been hypothesized that all TVTs start as linphocitoyd, become mixed and then plasmocytoid. The latter condition usually suggests the tumor presence for more than eight weeks, often being seen in non genitals. Accordingly, in the present case there was an agreement with the usual standard morfocelular and the extragenital aspect observed. This morphology suggested that the evolution of TVT showed up late. Although TVT is essentially related to the external reproductive organs, one should be alert to its exclusively extragenital presentation. This peculiar location can lead to a delayed diagnosis because of the similarity with affections of another nature.

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A biodiversidade vem sofrendo grande diminuição nas últimas décadas, apesar do incremento de algumas iniciativas, que visam o uso sustentável da diversidade biológica. A criação de bancos de germoplasma, os quais abrigam coleções-base para a conservação de ampla variabilidade genética vegetal e animal, surge como alternativa para manutenção da biodiversidade. O presente trabalho apresenta dados acerca da legislação vigente que regulariza a conservação e uso de recursos genéticos, bem como os aspectos técnicos relativos a formação de coleções de gametas e embriões, particularmente, visando a preservação de espécies silvestres no Brasil.

Biodiversity has suffered great loss in recent decades, despite the increased number of initiatives that aims the sustainable use of biological diversity. The creation of germplasm banks, which are collections that conserves the broad genetic variability of plants and animals, is an alternative to maintaining biodiversity. This paper presents data about the legislation that regulates the use and conservation of genetic resources, as well as the technical aspects of the formation of collections of gametes and embryos, particularly in the preservation of wild species in Brazil.

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A biodiversidade vem sofrendo grande diminuição nas últimas décadas, apesar do incremento de algumas iniciativas, que visam o uso sustentável da diversidade biológica. A criação de bancos de germoplasma, os quais abrigam coleções-base para a conservação de ampla variabilidade genética vegetal e animal, surge como alternativa para manutenção da biodiversidade. O presente trabalho apresenta dados acerca da legislação vigente que regulariza a conservação e uso de recursos genéticos, bem como os aspectos técnicos relativos a formação de coleções de gametas e embriões, particularmente, visando a preservação de espécies silvestres no Brasil.(AU)

Biodiversity has suffered great loss in recent decades, despite the increased number of initiatives that aims the sustainable use of biological diversity. The creation of germplasm banks, which are collections that conserves the broad genetic variability of plants and animals, is an alternative to maintaining biodiversity. This paper presents data about the legislation that regulates the use and conservation of genetic resources, as well as the technical aspects of the formation of collections of gametes and embryos, particularly in the preservation of wild species in Brazil.(AU)

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Visceral leishmaniasis (VL) is a major public health challenge in Brazil, especially in states where it is endemic. The aim of this study was to investigate the relationship of sand fly population density with environmental variables (temperature, rainfall and relative humidity) in urban areas of the city of Mossoró, Rio Grande do Norte, Northeastern Brazil. Sand flies were captured with Center Disease Control (CDC) traps installed monthly in the intra and peridomicile of three houses. Data analysis was based on the chi-square test and linear regression. A total of 7,347 sand flies were captured, being 93.85% Lutzomyia longipalpis and 6.15% Lutzomyia evandroi. Sand flies were more commonly found in the peridomicile and there was no difference between the number of males and females. The variables rainy season as well as relative humidity and rainfall, alone or together, did not have an effect on sand fly population density. However, high temperatures had a negative effect. The study of the behavior of sand flies in specific units of endemic areas can provide input to public health authorities for planning appropriate VL vector control measures.

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