RESUMO
Physical exercise has been shown to be an important means of prevention and treatment against many diseases. One factor is always highlighted the intensity of the activity. This study used an adapted protocol of high intensity interval training. Were evaluated biomarkers lactate and glucose. This study was approved by the Ethics Committee for the Use of Animals from the State University of Ceará registered under number 2542310/2015. The animals were previously adapted for 10 days with increasing speed up to 0.8km / h. The stress tests were done in steps of 3 minutes of intense activity for 2 minutes of rest. After determining the maximum capacity of the animal was made the training consisted of a 2 minute activity at 80% of maximum, followed by 4 minutes of rest. Before and after each exercise period, blood samples were collected for lactate tests, samples for glucose testing were collected just before the activity and after completion of all speed tests. They were obtained as results the increase of lactate and glucose levels in the post exercise. The lactate values and ranged from 0.5 mmol / L at rest and increased to an average of 1.7 mmol / L in 2.0 km / h. Blood glucose values were 120 mg / dL to about 170 mg / dl after training. Thereby indicating that the protocol used took the animals to a stress level higher than the rest, possibly due to intense physical activity.
Assuntos
Animais , Ratos , Exercício Físico , Glucose/metabolismo , Ácido Láctico/biossíntese , Ácido Láctico/metabolismo , Ácido Láctico/sangueRESUMO
Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...]
Assuntos
Animais , Ratos , Doença de Alzheimer , Peptídeos beta-Amiloides , Degeneração Neural , Doenças do Sistema Nervoso , MemóriaRESUMO
Alzheimer's disease (AD) was established as the neurodegenerative dementia, which leads to progressive and irreversible cognitive decline, memory loss, attention and judgment. It is characterized by molecular injuries resulting from the accumulation of charged neuritic plaques of β-amyloid and neurofibrillary tangles causing oxidative damage and inflammatory. Thus, the aim of this study was to evaluate the learning and memory of rats seven days after infusion of β-amiloide1-42. The β-amiloide1-42 was prepared in saline protein (1ug / uL) and incubated at 37 ° C for three days to form the aggregate. The animals were anesthetized with the combination of ketamine (100 mg / kg) and xylazine (10 mg / kg) intraperitoneally, and fixed in a stereotaxic route. The aggregate was infused directly in the hippocampus (5UL bilaterally) in the coordinates, AP: -3.5 mm; LL: ± 2.0 mm; DV: -3.5. The learning and memory was assessed using the Morris water maze. The apparatus consists of a circular water tank (120 cm diameter and 60 cm high) with an escape platform (12.5 cm in diameter and 38 cm high) positioned invisible to the mice 2 cm below the water level. The tank was divided by imaginary lines (N, S, L, and O) in four quadrants (1, 2, 3 and 4) and identified by the same geometrical symbols, which remained the same throughout the study. The animals were placed facing the geometric shapes and the exhaust time measured during four consecutive days (training). In the fifth and final day it was taken to the escape platform and computed the total residence time in the target quadrant (test). The maximum time for each step was 60s. This study was approved by Ethics Committee for Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015. To analyze the learning we used the ANOVA two way with Bonferroni post-test. To verify differences in memory between the groups we used the Student t test. P <0.05 significance level was adopted[...](AU)
Assuntos
Animais , Ratos , Doença de Alzheimer , Peptídeos beta-Amiloides , Memória , Degeneração Neural , Doenças do Sistema NervosoRESUMO
Physical exercise has been shown to be an important means of prevention and treatment against many diseases. One factor is always highlighted the intensity of the activity. This study used an adapted protocol of high intensity interval training. Were evaluated biomarkers lactate and glucose. This study was approved by the Ethics Committee for the Use of Animals from the State University of Ceará registered under number 2542310/2015. The animals were previously adapted for 10 days with increasing speed up to 0.8km \ h. The stress tests were done in steps of 3 minutes of intense activity for 2 minutes of rest. After determining the maximum capacity of the animal was made the training consisted of a 2 minute activity at 80% of maximum, followed by 4 minutes of rest. Before and after each exercise period, blood samples were collected for lactate tests, samples for glucose testing were collected just before the activity and after completion of all speed tests. They were obtained as results the increase of lactate and glucose levels in the post exercise. The lactate values and ranged from 0.5 mmol / L at rest and increased to an average of 1.7 mmol / L in 2.0 km / h. Blood glucose values were 120 mg / dL to about 170 mg / dl after training. Thereby indicating that the protocol used took the animals to a stress level higher than the rest, possibly due to intense physical activity.(AU)