RESUMO
Background: Mastitis is one of the diseases that cause damage to dairy cattle, and coagulase positive Staphylococcus (CPS),are its main etiological agents. The production of biofilms gives these microorganisms a physiological advantage, being animportant virulence factor, as it confers their resistance to the action of disinfectants used in hygiene procedures. Therefore,the objective of this study was to evaluate the capacity of CPS, isolated from milk samples from cases of bovine subclinicalmastitis in the Alagoas dairy basin, biofilm production and its sensitivity to disinfectant agents used before and after dipping.Materials, Methods & Results: In the Alagoas Milk Basin Region, 10 properties were selected from the municipalities ofBatalha, Cacimbinhas, Major Isidoro and Minador do Negrão, which used a mechanical milking system and supplied milk toa dairy under federal inspection. The California Mastitis Test (CMT) was performed on 1155 cows. The animals diagnosedwith subclinical mastitis were classified as: mild (+); moderate (++) and severe (+++). CMT positive samples were collectedfrom 2+, totaling 891 samples. After cleaning the papillary ostium, 2 to 5 mL of milk was collected from each fourth breastbefore milking began. Subsequently, 0.5 µL of each sample was streak plated by exhaustion on 5% Sheep Blood Agar andincubated at 37°C, with readings at 24 and 48 h. The macroscopic and microscopic characteristics of the colonies, catalaseand coagulase test were evaluated. Biofilm formation was determined by the microplate adhesion test. The efficacy of thesanitizers was evaluated with the active ingredients: lactic acid (2%), allantoin (0.05%), iodine (0.5%), chlorhexidine (2.0%),chlorine (2.5%), at times 15, 30 and 60 seconds. It was observed that 91.2% of the samples were able to form biofilm; Of these,22.2%, 29.6% and 48.2% were strong, moderate and weak producers, respectively. The sensitivity profile of chlorhexidine...(AU)
Assuntos
Leite/microbiologia , Biofilmes , Staphylococcus/imunologia , Mastite Bovina , Virulência , DesinfetantesRESUMO
Background: Mastitis is one of the diseases that cause damage to dairy cattle, and coagulase positive Staphylococcus (CPS),are its main etiological agents. The production of biofilms gives these microorganisms a physiological advantage, being animportant virulence factor, as it confers their resistance to the action of disinfectants used in hygiene procedures. Therefore,the objective of this study was to evaluate the capacity of CPS, isolated from milk samples from cases of bovine subclinicalmastitis in the Alagoas dairy basin, biofilm production and its sensitivity to disinfectant agents used before and after dipping.Materials, Methods & Results: In the Alagoas Milk Basin Region, 10 properties were selected from the municipalities ofBatalha, Cacimbinhas, Major Isidoro and Minador do Negrão, which used a mechanical milking system and supplied milk toa dairy under federal inspection. The California Mastitis Test (CMT) was performed on 1155 cows. The animals diagnosedwith subclinical mastitis were classified as: mild (+); moderate (++) and severe (+++). CMT positive samples were collectedfrom 2+, totaling 891 samples. After cleaning the papillary ostium, 2 to 5 mL of milk was collected from each fourth breastbefore milking began. Subsequently, 0.5 µL of each sample was streak plated by exhaustion on 5% Sheep Blood Agar andincubated at 37°C, with readings at 24 and 48 h. The macroscopic and microscopic characteristics of the colonies, catalaseand coagulase test were evaluated. Biofilm formation was determined by the microplate adhesion test. The efficacy of thesanitizers was evaluated with the active ingredients: lactic acid (2%), allantoin (0.05%), iodine (0.5%), chlorhexidine (2.0%),chlorine (2.5%), at times 15, 30 and 60 seconds. It was observed that 91.2% of the samples were able to form biofilm; Of these,22.2%, 29.6% and 48.2% were strong, moderate and weak producers, respectively. The sensitivity profile of chlorhexidine...
Assuntos
Biofilmes , Leite/microbiologia , Mastite Bovina , Staphylococcus/imunologia , Virulência , DesinfetantesRESUMO
Foodborne diseases represent a global public health issue and dairy products are closely related to it, since the quality of the milk produced in several Brazilian regions is unsatisfactory due to the presence of microorganisms in it. Listeria monocytogenes is a serious problem linked to food safety and, when it comes to milk, it represents a potential danger because it can withstand food storage temperatures, among other characteristics. The significant participation of Alagoas State in this the dairy production sector and the importance of producing safe food led to the aim of the present study, namely: investigating Listeria monocytogenes in expansion tank milk in Alagoas State counties. Milk samples were collected from tanks in 30 milk-processing unit suppliers. Next, they were taken to the Meat and Milk Inspection Laboratory (LICAL - UFRPE). The ISO 11290-1: 1996 / Amd.1: 2004 method, with adaptations, was used in the analyses. Bluish colonies, with or without halo formation, were identified according to their morpho-tintorial and biochemical characteristics. Listeria monocytogenes were detected in 20% of the samples (6/30), and such rate represents a public health risk. Thus, monitoring this microorganism and mastitis in the herd, as well as pre- and post-dipping, equipment and storage tank sanitation, and milk collection through refrigerated trucks at the appropriate time is a relevant procedure to prevent contamination and to assure safe food provision to the population.
Assuntos
Doenças Transmitidas por Alimentos/prevenção & controle , Leite/microbiologia , Listeria monocytogenes/crescimento & desenvolvimento , Microbiologia de Alimentos , Brasil , Indústria de Laticínios , Saúde PúblicaRESUMO
Foodborne diseases represent a global public health issue and dairy products are closely related to it, since the quality of the milk produced in several Brazilian regions is unsatisfactory due to the presence of microorganisms in it. Listeria monocytogenes is a serious problem linked to food safety and, when it comes to milk, it represents a potential danger because it can withstand food storage temperatures, among other characteristics. The significant participation of Alagoas State in this the dairy production sector and the importance of producing safe food led to the aim of the present study, namely: investigating Listeria monocytogenes in expansion tank milk in Alagoas State counties. Milk samples were collected from tanks in 30 milk-processing unit suppliers. Next, they were taken to the Meat and Milk Inspection Laboratory (LICAL - UFRPE). The ISO 11290-1: 1996 / Amd.1: 2004 method, with adaptations, was used in the analyses. Bluish colonies, with or without halo formation, were identified according to their morpho-tintorial and biochemical characteristics. Listeria monocytogenes were detected in 20% of the samples (6/30), and such rate represents a public health risk. Thus, monitoring this microorganism and mastitis in the herd, as well as pre- and post-dipping, equipment and storage tank sanitation, and milk collection through refrigerated trucks at the appropriate time is a relevant procedure to prevent contamination and to assure safe food provision to the population.(AU)