RESUMO
PURPOSE: To track the regeneration process of lateral gastrocnemius due to a muscle laceration in rats, and to treatment with plateletrich plasma (PRP). METHODS: Ultrasound (40 MHz) images were used for measuring pennation angle (PA), muscle thickness (MT) and mean pixel intensity, along with claudication scores, of treated (PRPG) and non-treated (NTG) groups of rats. RESULTS: NTG showed a PA increase for the non-injured leg (p<0.05) and a tendency of MT to increase, whereas for PRPG there were no differences. There was a progressive reduction of the claudication score for the PRPG group throughout the entire period, with an immediate difference after seven days (p<0.05), whereas the NTG had a significant reduction only at day 28 (p<0.05). CONCLUSION: It was observed a compensatory hypertrophic response due to the overload condition imposed to healthy leg for NTG that did not occur in PRPG, suggesting an accelerated repair process of the injured leg due to treatment, anticipating its use.
Assuntos
Lacerações/terapia , Músculo Esquelético/fisiologia , Músculo Esquelético/fisiopatologia , Plasma Rico em Plaquetas , Regeneração/fisiologia , Animais , Feminino , Seguimentos , Claudicação Intermitente/terapia , Microscopia Intravital/métodos , Lacerações/diagnóstico por imagem , Microscopia Acústica/métodos , Modelos Animais , Músculo Esquelético/diagnóstico por imagem , Distribuição Aleatória , Ratos WistarRESUMO
PURPOSE:To track the regeneration process of lateral gastrocnemius due to a muscle laceration in rats, and to treatment with plateletrich plasma (PRP).METHODS:Ultrasound (40 MHz) images were used for measuring pennation angle (PA), muscle thickness (MT) and mean pixel intensity, along with claudication scores, of treated (PRPG) and non-treated (NTG) groups of rats.RESULTS:NTG showed a PA increase for the non-injured leg (p<0.05) and a tendency of MT to increase, whereas for PRPG there were no differences. There was a progressive reduction of the claudication score for the PRPG group throughout the entire period, with an immediate difference after seven days (p<0.05), whereas the NTG had a significant reduction only at day 28 (p<0.05).CONCLUSION:It was observed a compensatory hypertrophic response due to the overload condition imposed to healthy leg for NTG that did not occur in PRPG, suggesting an accelerated repair process of the injured leg due to treatment, anticipating its use.(AU)
Assuntos
Animais , Ratos , Microscopia com Lâmpada de Fenda/veterinária , Lesões dos Tecidos Moles/cirurgia , Lesões dos Tecidos Moles/terapia , Lesões dos Tecidos Moles/veterinária , Plasma Rico em Plaquetas , Músculo Esquelético/lesões , Ultrassonografia/veterináriaRESUMO
PURPOSE: To track the regeneration process of lateral gastrocnemius due to a muscle laceration in rats, and to treatment with plateletrich plasma (PRP). METHODS: Ultrasound (40 MHz) images were used for measuring pennation angle (PA), muscle thickness (MT) and mean pixel intensity, along with claudication scores, of treated (PRPG) and non-treated (NTG) groups of rats. RESULTS: NTG showed a PA increase for the non-injured leg (p<0.05) and a tendency of MT to increase, whereas for PRPG there were no differences. There was a progressive reduction of the claudication score for the PRPG group throughout the entire period, with an immediate difference after seven days (p<0.05), whereas the NTG had a significant reduction only at day 28 (p<0.05). CONCLUSION: It was observed a compensatory hypertrophic response due to the overload condition imposed to healthy leg for NTG that did not occur in PRPG, suggesting an accelerated repair process of the injured leg due to treatment, anticipating its use.
Assuntos
Animais , Feminino , Regeneração/fisiologia , Músculo Esquelético/fisiologia , Músculo Esquelético/fisiopatologia , Lacerações/terapia , Plasma Rico em Plaquetas , Distribuição Aleatória , Seguimentos , Ratos Wistar , Músculo Esquelético/diagnóstico por imagem , Lacerações/diagnóstico por imagem , Modelos Animais , Microscopia Acústica/métodos , Microscopia Intravital/métodos , Claudicação Intermitente/terapiaRESUMO
This study used ultrasound biomicroscopy (UBM) to quantify the pennation angle (PA) and muscle thickness (MT) of rat skeletal muscle and evaluated the reliability and reproducibility of the method by statistical analysis, determining the coefficient of variation (CV), intraclass correlation coefficient (ICC) and typical error of measurement. A UBM system with a center frequency of 40 MHz was used to acquire images of the right lateral gastrocnemius of ten male Wistar rats on two different days and with two ankle positions (90° or 150°). Two independent measurements of the PA and MT were randomly performed in each of three picture frames. The analysis resulted in CVs of 10.47% and 4.81% for the PA and the MT, respectively, for the ankle at 90° and 9.24% and 5.98% for the ankle at 150°. Additionally, the ICC values ranged from 0.75 to 0.92 for the PA and 0.57 to 0.99 for the MT. Statistically significant differences between the ankle positions were observed for the PA (pâ=â0.00013). The reliability of the PA and MT measurements for the rat right lateral gastrocnemius, determined from the ultrasound biomicroscopy images, was high (>0.90) for the methodology proposed. This finding indicates the potential of ultrasound biomicroscopy for quantitative muscle characterization and the longitudinal examination of tissue adaptation to different conditions of use, disease and rehabilitation.
Assuntos
Microscopia Acústica/métodos , Músculo Esquelético , Animais , Masculino , Microscopia Acústica/instrumentação , Ratos , Ratos WistarRESUMO
Há, atualmente, na literatura, poucas pesquisas de confiabilidade e validação da quantificação de parâmetros de arquitetura muscular por ultrassonografia (US), relatando informações importantes como o erro típico da medida (ETM) e o coeficiente de correlação intraclasse (ICC). O objetivo do estudo foi aplicar a técnica de US para quantificar as medidas de comprimento da fibra (CF) e ângulo de penação (AP) dos músculos gastrocnêmios e determinar a confiabilidade e a reprodutibilidade dessas medidas. Duas imagens de cada gastrocnêmio de 19 indivíduos jovens e saudáveis foram adquiridas com um equipamento de ultrassom modo-B (7,5MHz), em duas visitas. CF e AP foram medidos cinco vezes nas duas imagens de cada indivíduo, totalizando vinte medidas de cada parâmetro para cada participante. A confiabilidade do total das 760 medidas foi determinada através do coeficiente de variação (CV), ICC e ETM. O CV do CF variou de 1,67 a 4,44 por cento e do AP de 3,8 a 11,62 por cento. Todos os ICC se encontram no intervalo de grande confiabilidade, com exceção do valor referente à medida interdias do AP do gastrocnêmio medial (0,67). Os dois valores mais altos de ETM (3,1º e 3,7º) correspondem aos dois valores inferiores do ICC (0,67 e 0,83), referentes às medidas repetidas interdias dos gastrocnêmios. As medidas de CF dos gastrocnêmios medial e lateral apresentaram ETM de 2,17mm e 2,25mm, respectivamente. Os resultados obtidos demonstraram que a metodologia utilizada para medição de CF e AP realizada em imagens de ultrassom apresentou alta confiabilidade.
There are few studies in the literature concerning the reliability and validity of the quantification of muscle architecture parameters using ultrasound that also report important information such as typical error of measurement (TEM) and intraclass correlation coefficient (ICC). The aim of this study was to use ultrasound imaging to quantify fiber length (FL) and pennation angle (PA) of the gastrocnemius muscle and to determine the reliability and reproducibility of these measurements. Two images of each gastrocnemius of 19 young healthy subjects were acquired using ultrasound equipment (7.5 MHz), in two visits. FL and PA were measured five times on each image, for a total of twenty measurements of each parameter. The overall reliability of the 760 measurements was determined through coefficients of variation (CV), ICC and TEM. The CV of FL ranged from 1.67 to 4.44 percent and from 3.8 to 11.62 percent for the PA. All ICC were within the high reliability range, with the exception of the between-visits PA value of the medial gastrocnemius (0.67). The two highest TEM values (3.1º and 3.7º) correspond to the two lowest ICC values (0.67 and 0.83), referring to repeated between-visit measurements of the medial and lateral gastrocnemius. FL measurements of the medial and lateral gastrocnemius had a TEM of 2.17 and 2.25 respectively. The results showed that ultrasound-based measurement of FL and PA exhibits high reliability.