RESUMO
The effect of paraprobiotic Lacticaseibacillus casei 01 inactivated by ohmic heating (8 V/cm, 95 °C/7 min, 60 Hz) whey-grape juice drink at the postprandial glycemia was evaluated. In vitro hypoglycemic activity was assessed by the α-glucosidase and α-amylase inhibition, while in vivo activity was determined using 15 healthy subjects, which consumed bread + probiotic whey drink, bread + paraprobiotic whey drink, and bread alone as a control. The probiotic and paraprobiotic grape-flavored whey drinks showed similar α-glucosidase and α-amylase inhibition (51.2 vs 51.8% and 43.2 vs 44.2%, respectively). The consumption of both paraprobiotic and probiotic whey drinks increased the incremental glucose rate when compared to the control due to the presence of sugar in its composition, without changes in the other parameters evaluated (maximum glucose value, glucose incremental percentage, and peak blood glucose time), showing a reduced glycemic response. In addition, the consumption of the paraprobiotic drink maintained the maximum glucose increase similar to the control, while an increase in this parameter was observed after the consumption of the probiotic drink. Therefore, the paraprobiotic grape-flavored whey drink may be an effective alternative to replace the probiotic product in reducing the postprandial glycemia in healthy individuals.
Assuntos
Vitis , Adulto , Glicemia , Calefação , Humanos , Período Pós-Prandial , Soro do LeiteRESUMO
Wasps are able to synthesize toxic compounds known as venoms, which form a part of a mechanism to overcome prey and also to defend their colonies. Study of the compounds that constitute these substances is essential in order to understand how this defense mechanism evolved, since there is evidence that the venoms can vary both intra- and interspecifically. Some studies have used liquid and gas chromatography as a reliable technique to analyze these compounds. However, the use of Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) to analyze the variations in venom's chemical profile has been proposed recently. This study evaluated whether the FTIR-PAS technique is effective for assessing the role of environmental factors on intra- and interspecific differences in the venom of the wasps Polybia paulista Von Ihering and Polybia occidentalis Olivier by FTIR-PAS. The colonies were collected in three municipalities of Mato Grosso do Sul, Brazil, in different types of environments. The results showed that the venoms of P. paulista and P. occidentalis differed significantly in profile. In addition, the intraspecific differences in the venom's chemical profile of P. paulista are related to the type of environment where they nested, regardless of the geographical distance between the nests. The FTIR-PAS technique proved to be reliable and effective to evaluate the variations in the venom's chemical profile in social wasps.
Assuntos
Peçonhas/química , Vespas , Animais , Brasil , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
BACKGROUND: Previous studies have shown that acupuncture and electroacupuncture (EA) are effective in the treatment of patients with low back pain. However, there is little evidence to support the use of one intervention over the other. The aim of this study is to compare the effect of acupuncture and electroacupuncture in the treatment of pain and disability in patients with chronic nonspecific low back pain. METHODS/DESIGN: The study design is a randomized controlled trial. Patients with nonspecific chronic low back pain of more than three months duration are recruited at Rehabilitation Center of Taboao da Serra - SP (Brazil). After examination, sixty-six patients will be randomized into one of two groups: acupuncture group (AG) (n = 33) and electroacupuncture group (EG) (n = 33). Interventions will last one hour, and will happen twice a week for 6 weeks. The primary clinical outcomes will be pain intensity as measured and functional disability. SECONDARY OUTCOMES: quality of pain, quality of life. perception of the overall effect, depressive state, flexibility and kinesiophobia. All the outcomes will be assessed will be assessed at baseline, at treatment end, and three months after treatment end. Significance level will be determined at the 5 % level. Results of this trial will help clarify the value of acupuncture and electroacupuncture as a treatment for chronic low back pain and if they are different. DISCUSSION: Results of this trial will help clarify the value of acupuncture needling and electroacupuncture stimulation of specific points on the body as a treatment for chronic low back pain. TRIAL REGISTRATION: Clinicaltrials.gov: NCT02039037 . Register October 30, 2013.
Assuntos
Terapia por Acupuntura/métodos , Dor Crônica/terapia , Eletroacupuntura/métodos , Dor Lombar/terapia , Terapia por Acupuntura/efeitos adversos , Brasil , Dor Crônica/diagnóstico , Dor Crônica/fisiopatologia , Protocolos Clínicos , Avaliação da Deficiência , Eletroacupuntura/efeitos adversos , Humanos , Dor Lombar/diagnóstico , Dor Lombar/fisiopatologia , Medição da Dor , Qualidade de Vida , Projetos de Pesquisa , Inquéritos e Questionários , Fatores de Tempo , Resultado do TratamentoRESUMO
A field experiment to assess the effects of continuous nutrient addition on the macrobenthic community was carried out on an estuarine mudflat on the northeast coast of Brazil. The experiment began on 5 October 2005 and ended on 8 February 2006. Macrofauna was compared at approximately four-week intervals in triplicate plots with three levels (Control - C, Low Dose - LD and High Dose - HD) of weekly fertilizer additions for 17 weeks. Inorganic fertilizer (N-P-K) was applied on nine randomly defined quadrangular plots (4m(2) each). All measurements were calculated from species abundances. Multivariate analyses as well as the univariate indices (richness, abundance and Shannon-Wiener index) showed statistically significant differences between the enriched and control areas during the period of the experiment. The expected gradual response based on the succession model of Pearson and Rosenberg was not observed. The nutrient doses used were high enough to cause severe decreases in abundance, richness and evenness, and an increase in dominance.
Assuntos
Ecossistema , Poluentes Ambientais , Estuários , Fertilizantes , Animais , Brasil , Análise Multivariada , Clima TropicalRESUMO
The purpose of the study was to compare hip agonist-antagonist isometric strength ratios between females with patellofemoral pain (PFP) syndrome and pain-free control group. One hundred and twenty females between 15 and 40 years of age (control group: n = 60; PFP group: n = 60) participated in the study. Hip adductor, abductor, medial rotator, lateral rotator, flexor, and extensor isometric strength were measured using a hand-held dynamometer. Comparisons in the hip adductor/abductor and medial/lateral rotator and flexor/extensor strength ratios were made between groups using independent t-tests. Group comparisons also were made between the anteromedial hip complex (adductor, medial rotator, and flexor musculature) and posterolateral hip complex (abductor, lateral rotator, and extensor musculature). On average, the hip adductor/abductor isometric strength ratio in the PFP group was 23% higher when compared with the control group (p = 0.01). The anteromedial/posterolateral complex ratio also was significantly higher in the PFP group (average 8%; p = 0.04). No significant group differences were found for the medial/lateral rotator ratio and flexor/extensor strength ratios. The results of this study demonstrate that females with PFP have altered hip strength ratios when compared with asymptomatic controls. These strength imbalances may explain the tendency of females with PFP to demonstrate kinematic tendencies that increase loading on the patellofemoral joint (i.e., dynamic knee valgus).
Assuntos
Quadril/fisiopatologia , Força Muscular , Músculo Esquelético/fisiopatologia , Síndrome da Dor Patelofemoral/fisiopatologia , Adolescente , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Contração Isométrica , Adulto JovemRESUMO
The aim of the present study was to investigate the ecological quality of tropical estuaries on the northeastern coast of Brazil using the AMBI. Macrofauna classification based on ecological groups was reviewed using the Indicator Value (IndVal) coefficient. The results indicate that the ecosystems exhibit some level of disturbance. Most sites are situated between slightly-moderately disturbed boundaries due to the higher proportion of Nematoda (assigned here as Ecological Group I) and of Oligochaeta and Tubificidae (both classified as Ecological Group V). The AMBI proved efficient in evaluating environmental status, although the applicability of this index requires adjustments regarding some species in ecological groups. The present study also highlights the merits of the IndVal method for examining the assignments of species/taxa to an ecological group and demonstrates the validity of this coefficient is an assessment tool. Moreover, the complementary use of different methods is recommended for the assessment of ecosystem quality.
Assuntos
Organismos Aquáticos/classificação , Biodiversidade , Monitoramento Ambiental/métodos , Invertebrados/classificação , Animais , Organismos Aquáticos/crescimento & desenvolvimento , Brasil , Conservação dos Recursos Naturais , Ecossistema , Invertebrados/crescimento & desenvolvimentoRESUMO
The presence of a riparian forest is one of the main factors that act directly on the ecology of a fluvial system, and the relation of the forest and the lotic environments might have an important influence on the distribution of the macroinvertebrates. In this context, the benthic macroinvertebrate communities in four low-order streams in São Paulo (Brazil) were analysed, with the aim of assessing the state of recovery of the surrounding forest fragments. The benthic organisms were sampled in the winter, a period of low rainfall. Of the 6,331 specimens of macroinvertebrates collected, 124 taxa belonging to 48 families were identified. The results showed greater diversity in the Canchim Farm streams and greater abundance in the Lake Park streams. Cluster analysis showed that the stream Canchim distanced itself from the others, being considered reference.
Assuntos
Invertebrados/classificação , Árvores , Animais , Brasil , Conservação dos Recursos Naturais , Monitoramento Ambiental/métodos , Densidade Demográfica , Rios , Estações do AnoRESUMO
Molecular study of aerolysin and cytotonic enterotoxin genes by PCR and colony blot hybridization was performed in 117 strains of Aeromonas spp. isolated from different sources. Homogeneous distribution of these genes in A. hydrophila complex strains was observed. For A. caviae and A. sobria complex strains, aerolysin genes were more frequent than cytotonic enterotoxins genes. Of 64 A. caviae complex strains, only one (1.5%) amplified the 451 bp product for the aer gene, however, the same primers detected a 400 bp product in 50 (78%) strains. This product was sequenced and had two short regions with homology to several hemolysin genes. The genotype aer (+)/aerA(+)/hly (+)/ast (+)/alt (+) was detected in six A. hydrophila strains from food and environmental source. The most common genotype found in A. hydrophila strains was hly (+) (85%) and aerA(+) (78.7%), while in A. caviae complex strains was aerA(+) (32.8%). All A. veronii complex sobria strains were aer (+)/aerA(+). All A. caviae and A. hydrophila were positive when tested with aer probe using the colony blot test. Thirty-seven percent of A. hydrophila and 53% of A. caviae tested were positive for ast probe. Eighty-nine percent of samples were cytotoxic in Vero cells. Our data demonstrated that Aeromonas spp. can harbor and express virulence genes and reinforce the potential of Aeromonas as a human pathogen.
Assuntos
Aeromonas/isolamento & purificação , Aeromonas/patogenicidade , Toxinas Bacterianas/genética , Microbiologia Ambiental , Microbiologia de Alimentos , Infecções por Bactérias Gram-Negativas/microbiologia , Fatores de Virulência/genética , Brasil , Morte Celular , Genótipo , Hemólise , Humanos , Hibridização de Ácido Nucleico , Reação em Cadeia da PolimeraseRESUMO
Os autores fazem uma revisão bibliográfica sobre os tumores da Papila de Vater, sua classificação, apresentação clínica, diagnóstico e estadiamento, assim como as alternativas de procedimentos cirúrgicos